Jian J.,Guilin Medical University |
Xuan F.,Guangxi Medical University |
Qin F.,Guangxi Medical University |
Huang R.,Guangxi Medical University
Drug Design, Development and Therapy | Year: 2015
This study aimed to determine the effects of Bauhinia championii flavone (BCF) on myocardial ischemia/reperfusion injury (MI/RI) in rats and to explore potential mechanisms. The MI/RI model in rats was established by ligating the left anterior descending coronary artery for 30 minutes, then reperfusing for 3 hours. BCF at 20 mg/kg was given 20 minutes prior to ischemia via sublingual intravenous injection, with 24 μg/kg phosphoinositide 3-kinase inhibitor (PI3K; wortmannin) as a control. The creatine kinase-MB and nitric oxide content were assessed by colorimetry. The levels of mitochondrial permeability transition pores and tumor necrosis factor alpha were determined by an enzyme-linked immunosorbent assay. Cardiomyocyte apoptosis was detected by the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Additionally, the expression of PI3K, endothelial nitric oxide synthase, caspase-3, and Beclin1 was analyzed by fluorescence quantitative polymerase chain reaction and Western blotting, respectively. Akt and microtubule-associated protein 1 light chain 3-II protein levels were also evaluated. Pretreatment with BCF significantly decreased the levels of creatine kinase-MB, tumor necrosis factor alpha, and mitochondrial permeability transition pores, but increased the nitric oxide content. Furthermore, BCF inhibited apoptosis, downregulated caspase-3, Beclin1, and microtubule-associated protein 1 light chain 3-II, upregulated PI3K, and increased the protein levels of phosphorylated Akt and endothelial nitric oxide synthase. However, all of the previously mentioned effects of BCF were blocked when BCF was coadministered with wortmannin. In conclusion, these observations indicated that BCF has cardioprotective effects against MI/RI by reducing cell apoptosis and excessive autophagy, which might be related to the activation of the PI3K/Akt signaling pathway. © 2015 Jian et al.
Cheng J.,Guilin Medical University |
Cheng J.,Tsinghua University |
Du J.,Guilin Medical University
CrystEngComm | Year: 2012
A simple and low-cost method to synthesize Ge nanoparticles/graphene (Ge NPs/GR) nanocomposites under mild conditions was developed. The reduction of sugarcane bagasse (SB) derived graphene oxide nanosheets was accompanied by generation of Ge NPs in one step. The resulting nanocomposites exhibit high specific capacity and superior capacity retention of 90% after 15 cycles.
Zou D.,Guilin Medical University |
Xie A.,Guangxi Medical University
Current Drug Metabolism | Year: 2013
Polyphenols are the most abundant antioxidants. Polyphenols are known to non-covalent interact with plasma proteins in blood through hydrophobic or hydrophilic interactions. It was found that the effect of polyphenol-plasma protein interaction (PpPI) on the bioavailability of polyphenols is not equivocal. Because the conclusion of individual reports are contradictory to each other; therefore, it is very difficult to give a univocal comment on the influence of PpPI on antioxidant property of polyphenols. The influence of PpPI on the antioxidant activity of polyphenols is decided by the antioxidant assay, the structure characteristics of polyphenols, as well as the proteins. This mini review mainly focused on the influence of PpPI on the antioxidant properties of polyphenols. © 2013 Bentham Science Publishers.
Liu C.-M.,Johns Hopkins University |
Wang R.-Y.,Johns Hopkins University |
Wang R.-Y.,Guilin Medical University |
Saijilafu,Johns Hopkins University |
And 3 more authors.
Genes and Development | Year: 2013
Regulated gene expression determines the intrinsic ability of neurons to extend axons, and loss of such ability is the major reason for the failed axon regeneration in the mature mammalian CNS. MicroRNAs and histone modifications are key epigenetic regulators of gene expression, but their roles in mammalian axon regeneration are not well explored. Here we report microRNA-138 (miR-138) as a novel suppressor of axon regeneration and show that SIRT1, the NAD-dependent histone deacetylase, is the functional target of miR-138. Importantly, we provide the first evidence that miR-138 and SIRT1 regulate mammalian axon regeneration in vivo. Moreover, we found that SIRT1 also acts as a transcriptional repressor to suppress the expression of miR-138 in adult sensory neurons in response to peripheral nerve injury. Therefore, miR-138 and SIRT1 form a mutual negative feedback regulatory loop, which provides a novel mechanism for controlling intrinsic axon regeneration ability. © 2013 by Cold Spring Harbor Laboratory Press.
Wang J.,Guilin Medical University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2011
To investigate the effect of curcumol on the proliferation, apoptosis and the expression NF-κB in nasopharyngeal carcinoma cell line CNE-2. CNE-2 cells were treated with curcumol at different concentration(12.5, 25, 50, 100 mg/L) and the control group; the effect of proliferation was detected by MTT method; the apoptosis was analyzed by hoechst 33342 flourescence staning and flow cytometry; the expression of NF-κB was detected with western blotting. After treated with curcumol, CNE-2 cell's proliferation was significantly reduced (P<0.01) and its apoptosis was increased as the curcumol concentration rising, the apoptosis of 100 mg/L curcumol group even can reach to 45.5% and it was a significantly difference compared with control group (P<0.01); the expression of NF-κB was down regulated as raising the curcumol concentration, there was a significantly difference compared with control group (P<0.01). Curcumol is capable of significantly inhibiting proliferation and inducing apoptosis of CNE-2' cells in vitro, the mechanism of curcumol anti-tumor may be related to the down regulated of the NF-κB protein level.
Li R.,Guilin Medical University
Pancreas | Year: 2015
OBJECTIVE: The adult pancreatic duct system accommodates endocrine cells that have the potential to produce insulin. Here we report the characterization and distribution of insulin-immunoreactive cells and endocrine cells within the ductal units of adult human pancreas. METHODS: Sequential pancreas sections from 12 nondiabetic adults were stained with biomarkers of ductal epithelial cells (cytokeratin 19), acinar cells (amylase), endocrine cells (chromogranin A; neuron-specific enolase), islet hormones (insulin, glucagon, somatostatin, pancreatic polypeptide), cell proliferation (Ki-67), and neogenesis (CD29). RESULTS: The number of islet hormone–immunoreactive cells increased from large ducts to the terminal branches. The insulin-producing cells outnumbered endocrine cells reactive for glucagon, somatostatin, or pancreatic polypeptide. The proportions of insulin-immunoreactive count compared with local islets (100% as a baseline) were 1.5% for the main ducts, 7.2% for interlobular ducts, 24.8% for intralobular ducts, 67.9% for intercalated ducts, and 348.9% for centroacinar cells. Both Ki-67– and CD29-labeled cells were predominantly localized in the terminal branches around the islets. The terminal branches also showed cells coexpressing islet hormones and cytokeratin 19. CONCLUSIONS: The adult human pancreatic ducts showed islet hormone–producing cells. The insulin-reactive cells predominantly localized in terminal branches where they may retain potential capability for β-cell neogenesis. Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.
Chen J.,Guilin Medical University |
Sun L.,Guilin Medical University
Hormone and Metabolic Research | Year: 2012
Formononetin is one of the main active components of red clover plants, and considered as a phytoestrogen. Its pharmacological effects in vivo may be either estrogenic or anti-estrogenic, mainly depending upon the estrogen levels. Our recent studies suggested that formononetin inactivated IGF1/IGF1R-PI3K/Akt pathways and decreased cyclin D1 mRNA and protein expression in human breast cancer cells in vitro and in vivo. In the present study, we further investigated the molecular mechanisms involved in the induced apoptosis effect of formononetin on breast cancer cells. Our results suggested that formononetin inhibited the proliferation of ER-positive MCF-7 cells and T47D cells. In contrast, formononetin could not inhibit the cell of growth of ER-negative breast cancer cells such as MDA-MB-435 S cells. We further found that formononetin activated MAPK signaling pathway in a dose-dependent manner, which resulted in the increased ratio of Bax/Bcl-2, and induced apoptosis on MCF-7 cells. However, when MCF-7 cells were pretreated with p38MAPK inhibitor SB203580 before formononetin, apoptosis induced by formononetin was significantly attenuated. Thus, we conclude that the induced apoptosis effect of formononetin on human breast cancer cells were related to Ras-p38MAPK pathway. Considering that red clover plants are widely used clinically, our results provide the foundation for future development of formononetin for treatment of ER-positive breast cancer. © Georg Thieme Verlag KG · Stuttgart.
Li Z.,Guilin Medical University
Molecular Biology Reports | Year: 2013
Recurrent or metastatic osteosarcomas remain a challenging malignancy to treat. Therefore, development and testing of novel therapeutic strategies to target these patients are needed. Adoptive cellular therapy strategies are being evaluated intensively as a novel therapeutic strategy for cancer. Unlike αβ T cells requiring antigen processing and MHC-restricted peptide displayed by antigen-presenting cells, γδ T cells exhibit the potent MHC-unrestricted lytic activity against various tumors in vitro and in vivo. The recent considerable success of γδ T cell-based immunotherapy in lung metastasis of renal cell carcinoma warrants further efforts to apply this treatment to other cancers including osteosarcoma, especially recurrent and metastatic osteosarcomas. In this review, we summarize the available evidence on γδ T cell-based immunotherapy for osteosarcoma that has been achieved to date. More importantly, we discuss potential strategies of the combination of expanded γδ T cells and bisphosphonates, and modification and expansion of αβ TCR modified γδ T cells for improving its efficacy for the treatment of osteosarcoma. © 2012 Springer Science+Business Media Dordrecht.
Tan Q.-G.,CAS Kunming Institute of Botany |
Tan Q.-G.,Guilin Medical University |
Luo X.-D.,CAS Kunming Institute of Botany
Chemical Reviews | Year: 2011
Some of the important naturally occurring limonoids from Meliaceae discovered in the last six decades, from 1942 to June 30, 2010, and their various bioactivities, are discussed. The cedrelone-class limonoids are characterized as the 5,6-enol-7-one derivatives and Burke et al. presented the chemical correlation of anthothecol with hirtin. Under mildly acid conditions, the first stage of the ring-opening of havanensin gives a 15-hydroxy-14- carbonium ion, which then either undergoes Wagner-Meerwein rearrangement or loses a proton to give a 15-ketone enolate and involves participation of the oxygenated function at C-7. The photooxidation of salannin and nimbin by UV light in the presence of oxygen led to more polar unstable intermediates that rearranged on silica gel to two final products in which the furan ring had been oxidized to isomeric hydroxybutenolides.
Qiu X.Q.,Guilin Medical University
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2011
To investigate the association between single nucleotide polymorphisms (SNPs) in cytokine IL-6, IL-10 genes and HBV-related hepatocellular carcinoma (HCC). A hospital-based case-control study was conducted in 381 cases with HBV-related HCC, 340 HBsAg carriers and 359 non-tumor controls. Genotypes of -572 site of IL-6 gene and -819, -592 sites of IL-10 gene were determined by real-time polymorphism chain reaction. Unconditional logistic regression was used to estimate the odds ratios (ORs) and 95 confidence intervals (CIs). For the G/C alleles of -572 loci on IL-6 gene, there were significant differences between the three groups (P < 0.05). Compared with CC genotype, GG genotype increased the risk of HBV infection (OR = 2.171, 95%CI: 1.068 - 4.415), but did not seem to be associated with HCC. For the alleles of -819 and -592 site of IL-10 gene, there were significant differences between the three groups (P < 0.05). Compared with CC genotype, TT genotype increased the risks of both HCC (OR = 2.791, 95%CI: 1.326 - 5.874), and HCC in HBsAg carriers (OR = 3.522, 95%CI: 1.707 - 7.266). When compared with CC genotype on -592 site, the AA genotype reduced the risk of both HCC (OR = 0.389, 95%CI: 0.173 - 0.875), and HCC in HBsAg carriers (OR = 0.336, 95%CI: 0.154 - 0.734). The SNPs in -572 site of IL-6 gene might be associated with the risk of HBV infection. The SNPs in -819 site of IL-10 gene increased the risk of HCC, but -592 site of IL-10 gene decreased the risk of HCC.