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Lei J.,Jinan University | Lei J.,Guangzhou Trauer Biotechnology Co. | Liu X.-Z.,Guangzhou Trauer Biotechnology Co. | Chen D.-C.,Guangzhou Trauer Biotechnology Co. | And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Collagen has been shown to promote the restoration of damaged tissue wound and to accelerate wound healing. OBJECTIVE: To observe the effect of collagen gel dressing on defect healing of diabetic skin and its role to promote capillary regeneration. METHODS: Forty of 160 Sprague-Dawley rats were used as normal controls, and the other 120 rats were used to make diabetic models with intraperitoneal injection of streptozotocin. After 9 weeks, 120 model rats were randomly divided into model group, positive control group and experimental group. Then full-thickness skin wounds were made on the back of all rats. The normal group and model group were externally treated with vaseline. Experimental group was externally treated with collagen gel dressing, and recombinant human epidermal growth factor gel was used in the positive control group. Finally, the healing area of wounds and newborn capillaries were observed. RESULTS AND CONCLUSION: At 1 week of administration, animals in the normal control group showed faster wound healing, but the wound healing rates in the other groups were changed insignificantly; at 2 weeks of administration, the healing rate of the normal control group was fastest, and the nonhealing area in the model group was larger than that in the positive control and experimental groups; at 3 weeks of administration, the nonhealing area of the normal control and experimental groups was smaller than that of the model and positive control groups; at 4 weeks of administration, the nonhealing area of the normal control and positive control groups was smaller than that of the model and experimental groups. In addition, wound healing was better in the experimental group than the positive control group at 2 and 3 weeks of administration; while the number of capillaries in the experimental group was more than that of the other three groups at 1, 2, 3 weeks of administration. These results suggest that collagen gel dressings can promote healing of diabetic wounds and improve healing quality. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research All Rights Reserved.

Chi Y.-Y.,Guangdong Medical Collagen Engineering and Technology Research Center | Chi Y.-Y.,Guangzhou Trauer Biotechnology Co. | Le Y.-J.,Guangdong Medical Collagen Engineering and Technology Research Center | Le Y.-J.,Guangzhou Trauer Biotechnology Co. | And 5 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Collagen sponges are applied for hemostatic use, wound healing, and residual cavity filling, which have great values in clinical application and scientific research. OBJECTIVE: To investigate the biological properties, biocompatibility and biodegradability of collagen sponges in vivo. METHODS: The spatial structure, pore diameter and porosity of collagen sponges were characterized by scanning electron microscopy. Transmission electron microscopy was used to observe the conformation of collagen sponges. The secondary structure and thermal denaturation temperature of collagen sponges were analyzed by circular dichroism spectrum. Collagen sponges were implanted intramuscularly into the spinal cord of New Zealand rabbits to observe the degradation and absorption and histological changes in vivo. RESULTS AND CONCLUSION: Collagen sponges had porous structure with varying pore sizes ranging 40-150 μm, the mean pore size of 100 μm, the thickness wall of 1 μm, and a porosity of approximately 95.8%. Collagen sponges had a typical porous structure and periodic light and dark zones. The solution of collagen sponges had a weak positive band near 220 nm and an intense negative band near 206 nm, which indicated a classic triple helix. And the secondary structure and thermal stability of collagen sponges were similar to that of liquid collagen. Collagen sponges began to degrade at 4 weeks, and remained 20% at 12 weeks. These sponges had been associated with foreign body response and inflammation within 2 weeks after implantation. With wound healing, inflammatory reactions gradually reduced and disappeared. During the implantation and degradation of sponges, no significant fibrous capsule formed and no tissue necrosis occurred at implantation site, indicating that collagen sponges have good performance in bioactivity, biocompatibility and degradation. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All Rights Reserved.

Tong G.,Guangzhou Trauer Biotechnology Company
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi | Year: 2012

This paper presents a 3mm fracture model of radial bone of rabbits using operation method. The bone defect was treated with collagen sponge with different pH and different content. After the operation, the body temperature, blood routine measurement, serum antibody, X-ray examination and histological observation in each group were examined to evaluate and study the curative effect and safety of collagen sponge. Collagen sponge had a good result of safety, but there was slightly change in blood routine, serum antibody, and histological observation, etc, with the pH changing and different content of collagen. The results showed that there was no obvious influence of safety to tissues after treatment of the collagen sponge at different pH implanted into bone defect. Collagen sponge at lower pH could promote the healing of bone defect partly, while the safety of collagen sponge with lower content was better.

Zhao P.,Guangzhou Trauer Biotechnology Co. | Lei J.,Guangzhou Trauer Biotechnology Co. | Chen D.-c.,Guangzhou Trauer Biotechnology Co. | Huang D.-j.,Guangzhou Trauer Biotechnology Co.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Collagen is a kind of natural material, which has a low immune, good cell compatibility and tissue compatibility. It can promote the generation of vascular and plays an extremely important role in cell differentiation, tissue repair and the normal nutrition to organs. OBJECTIVE: To evaluate the repair function of collagen spray on mechanical damaged nasal mucosa in rabbits. METHODS: Nasal mucosa mechanical damage model was prepared. Collagen sprays were given to the treatment group in the left nose, and the left nose of model group was treated with the same amount of saline. The repair situation and pathomorphology of the wounded tissue were observed. RESULTS AND CONCLUSION: Collagen spray had no excitant. The goblet cell count and submucosal glands in the treatment group were slightly decreased than the model group. The rate of submucosal edema in the treatment group was significantly different from that in the model group (P < 0.01). Under light microscope, there was a decrease of fresh repaired epithelial goblet cells, the submucosal glands were small and well-regulated, and its secretion was unobstructed; there were no obvious edema, and the glands were dense; none of the above could be seen in the control group. The collagen spray had a clear repair function on mucosal injury, the infiltrating phenomenon of inflammatory cells was reduced in the mucosal lamina propria, the performance of diffuse submucosal edema was not obvious, and the glandular secretion was in good condition.

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