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Chen G.,Guangzhou Panyu Central Hospital | Ren C.,Nankai University | Wang L.,Nankai University | Xu B.,Hong Kong University of Science and Technology | And 2 more authors.
Chinese Journal of Chemistry | Year: 2012

Enzyme-responsive hydrogels have great potential in applications of controlled drug release, tissue engineering, etc. In this study, we reported on a supramolecular hydrogel that showed responses to two enzymes, phosphatase which was used to form the hydrogels and esterase which could trigger gel-sol phase transitions. The gelation process and visco-elasticity property of the resulting gel, morphology of the nanostructures in hydrogel, and peptide conformation in the self-assembled nanostructure were characterized by rheology, transmission electron microscope (TEM), and circular dichroism (CD), respectively. Potential application of the enzyme-responsive hydrogel in drug release was also demonstrated in this study. Though only one potential application of drug release was proved in this study, the responsive hydrogel system in this study might have potentials for the applications in fields of cell culture, controlled-drug release, etc. Copyright © 2012 SIOC, CAS, Shanghai & WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source

Hong Y.,Guangzhou Panyu Central Hospital | Yu S.-T.,Sun Yat Sen University | Cai Q.,Sun Yat Sen University | Liang F.-Y.,Sun Yat Sen University | And 2 more authors.
European Archives of Oto-Rhino-Laryngology | Year: 2016

The aim of this study was to evaluate the safety, feasibility, effectiveness, and cosmesis of a gasless endoscopic-assisted thyroidectomy via the anterior chest in patients with Graves’ disease. We retrospectively reviewed 38 patients with Graves’ disease treated with thyroidectomy from November 2007 to June 2015. We analyzed clinical characteristics of patients, type of operation, operative indications, operative duration, length of postoperative hospital stay, and postoperative complications. The thyroidectomies were classified as total thyroidectomy (n = 12) or near-total thyroidectomy with a remnant of <1 g (n = 26). Surgical indications were recurrence after antithyroid drugs (ATDs) and unwillingness to undergo radioiodine therapy (n = 27), local compressive symptoms (n = 2), adverse drug reactions to ATDs (n = 5), and patient’s preference (n = 4). Mean resection weight was 71.7 ± 16.2 g (range 44–109 g), mean operative duration 87.7 ± 17.3 min (range 66–136 min), intraoperative blood loss 70.6 ± 11.3 mL (range 43–92 mL), and drainage was 42.0 ± 8.5 mL (range 20–62 mL). Temporary postoperative recurrent laryngeal nerve palsy and temporary hypoparathyroidism occurred in 3 cases (7.89 %) each. Mean hospital stay was 2.5 ± 0.3 days (range 2–4 days). There was no recurrence of hyperthyroidism over the follow-up period of for 68.1 ± 5.6 months (range 6–89 months). All patients were satisfied with their cosmetic results. Gasless endoscopic-assisted thyroidectomy via the anterior chest approach for Graves’ disease is a safe, feasible, and effective and provides an excellent cosmetic outcome procedure. It is a valid option in appropriately selected patients. © 2016 Springer-Verlag Berlin Heidelberg Source

Kong N.,Southern Medical University | Lu X.,Southern Medical University | Li B.,Guangzhou Panyu Central Hospital
BMC Molecular Biology | Year: 2014

Background: Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells. Results: Rat RGC-5 cells were cultured in plates and HO was added to induce oxidative stress. TUNEL assay and qRT-PCR showed HO induced apoptosis and up-regulated miR-100 in a dose-dependent manner in RGC-5 cells. Conversely, lentiviral-mediated miR-100 down-regulation protected HO induced apoptosis, promoted neurite growth and activated AKT/ERK and TrkB pathways through phosphorylation. Luciferase assay confirmed that IGF1R was directly regulated by miR-100 in RGC-5 cells, and siRNA-mediated IGF1R knockdown activated AKT protein through phosphorylation, down-regulated miR-100, therefore exerted a protective effect on RGC-5 apoptosis. Conclusion: Down-regulating miR-100 is an effective method to protect HO induced apoptosis in RGC-5 cells, possible associated with IGF1R regulation. © Kong et al. Source

Chen H.,Guangzhou Panyu Central Hospital | Chen H.,Jinan University | Jiang J.,Peking University | Gao J.,University of Hong Kong | And 7 more authors.
Journal of Computer Assisted Tomography | Year: 2014

OBJECTIVE: The objective of this study was to compare the accuracy of calculating the primary tumor volumes using a gradient-based method and fixed threshold methods on the standardized uptake value (SUV) maps and the net influx of FDG (Ki) maps from positron emission tomography-computed tomography (PET-CT) images. MATERIALS AND METHODS: Newly diagnosed patients with head and neck cancer were recruited, and dynamic PET-CT scan and T2-weighted magnetic resonance imaging were performed. The maps of Ki and SUV were calculated from PET-CT images. The tumor volumes were calculated using a gradient-based method and a fixed threshold method at 40% of maximal SUV or maximal Ki. Four kinds of volumes, VOLKi-Gra (from the Ki maps using the gradient-based method), VOLKi-40% (from the Ki maps using the threshold of 40% maximal Ki), VOLSUV-Gra (from the SUV maps using the gradient-based method), and VOLSUV-40% (from the SUV maps using the threshold of 40% maximal SUV), were acquired and compared with VOLMRI (the volumes acquired on T2-weighted images) using the Pearson correlation, paired t test, and similarity analysis. RESULTS: Eighteen patients were studied, of which 4 had poorly defined tumors (PDT). The positron emission tomography-derived volumes were as follows: VOLSUV-40%, 2.1 to 41.2 cm (mean [SD], 12.3 [10.6]); VOLSUV-Gra, 2.2 to 28.1 cm (mean [SD], 13.2 [8.4]); VOLKi-Gra, 2.4 to 17.0 cm (mean [SD], 9.5 [4.6]); and VOLKi-40%, 2.7 to 20.3 cm (mean [SD], 12.0 [6.0]). The VOLMRI ranged from 2.9 to 18.1 cm (mean [SD], 9.1 [3.9]). The VOLKi-Gra significantly correlated with VOLMRI with the highest correlation coefficient (PDT included, R = 0.673, P = 0.002; PDT excluded, R = 0.841, P < 0.001) and presented no difference from VOLMRI (P = 0.672 or 0.561, respectively, PDT included and excluded). The difference between VOLKi-Gra and VOLMRI was also the smallest. CONCLUSIONS: The tumor volumes delineated on the Ki maps using the gradient-based method are more accurate than those on the SUV maps and using the fixed threshold methods. © 2014 by Lippincott Williams & Wilkins. Source

Chen G.,Guangzhou Panyu Central Hospital | Hua Y.,Southern Medical University | Ou C.,Southern Medical University | Zhang X.,Nankai University | And 4 more authors.
Chemical Communications | Year: 2015

We report the design and synthesis of a light-up probe of DBT-2(EEGK-maleimide), which can serve as a unique probe for selectively detecting protein thiols in various environments, including aqueous solutions, bacteria and live cells. © 2015 The Royal Society of Chemistry. Source

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