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Zhao L.-N.,Sun Yat Sen University | Han W.-Y.,Sun Yat Sen University | Lu L.,Guangzhou Municipal Twelfth Peoples Hospital | Yu T.,Sun Yat Sen University | And 5 more authors.
Medical Science Monitor | Year: 2014

Background: Autoimmune pancreatitis (AIP) is a distinct type of pancreatitis associated with a presumed autoimmune mechanism. The aim of this study was to analyze the clinical features and expressions of forkhead box P3 (Foxp3) and interleukin-17 (IL-17) in type 1 AIP in China and to identify factors for diferentiation of AIP from non-AIP chronic pancreatitis (CP).Material/Methods: We retrospectively reviewed pancreatic specimens with diagnosis of type 1 AIP and non-AIP CP at Sun Yat-Sen Memorial Hospital in China from January 2000 to December 2013. The clinical symptoms, serological data, imaging findings, histopathology, and immunohistochemical findings of Foxp3 and IL-17 in the 2 groups were analyzed.Results: Twenty-nine patients with type 1 AIP and 20 patients with non-AIP CP were enrolled. Obstructive jaundice was more common in type 1 AIP than in non-AIP CP (62.1% vs. 30.0%, P=0.042). The difuse or segmental enlargement of the pancreas was more frequent in type 1 AIP than in non-AIP CP (72.4% vs. 40.0%, P=0.038). Histopathology of type 1 AIP presented dense lymphoplasmacytic infltration, “snowstorm-like” fbrosis and abundant immunoglobulin (Ig) G4+ cells. Foxp3+ cells were more frequently observed in type 1 AIP than in non-AIP CP. IL-17+ cell infltration was similar between the 2 groups. Furthermore, a positive correlation was found between Foxp3+ and IgG4+ cell counts in the pancreas of patients with type 1 AIP.Conclusions: Type 1 AIP has distinctive symptoms, image, and pathological characteristics, which could be used for difer-entiation from non-AIP CP. Foxp3+ cells might be helpful to distinguish type 1 AIP from non-AIP CP. © Med Sci Monit, 2014.

Chen L.-Z.,Guangzhou Municipal Twelfth Peoples Hospital | Chen J.-Y.,Guangzhou Municipal Twelfth Peoples Hospital | Wu J.-M.,Guangzhou Municipal Twelfth Peoples Hospital | Yu W.,Guangzhou Municipal Twelfth Peoples Hospital | Zhan Y.,Guangzhou Municipal Twelfth Peoples Hospital
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: Previous studies have shown that bone marrow mesenchymal stem cells (BMSCs) can differentiate into vascular endothelial cells, and then further differentiate into new blood capillary, resulting in forming new collateral branch and in improving blood supply in local site, which can provide new ideas for treatment of ischemic lower extremity disorders. OBJECTIVE: To observe clinical effects of autologous BMSCs transplantation on the treatment of severe diabetic foot. METHODS: One patient with type 2 diabetes and severe diabetic foot ulcers was included in this research. BMSCs were isolated and expanded from 50 mL bone marrow aspirates through lower-extremity artery CT vascular reconstruction, which suggested lower-extremity angiosclerosis, stenosis and occlusion. Mononuclear cells were harvested by density gradient centrifugation, and BMSCs were isolated and purified using adherent culture method, and then infused into patients via intravenous infusion following microbiological detection. At 3 and 6 months, 7 and 30 days following transplantation, clinical symptoms such as pain, cold sensation, intermittent claudication, ankle-brachial index (ABI), ulcer area and gangrene were observed. Improvement and blood flow in the lower extremity and artery lateral branch reconstruction were measured by color Doppler flow imaging and CT scan. RESULTS AND CONCLUSION: At 3 months following transplantation, pain and cold sensation significantly relieved and numbness was improved. At 6 months after transplantation, pain relief was achieved, ABI significantly increased, foot ulcers healed completely, lower extremity was maintained, and foot function in walking was well preserved. New vessels formation was visible and blood flow was significantly improved by color Doppler flow imaging and CT scan. No heart/live/lung/kidney function injury was observed during the transplantation. The patient was followed up for 14 months, no transplantation- relatedcomplications were observed. Autologous BMSCs transplantation provides a new way for the therapy of diabetic foot.

Yu W.,Guangzhou Municipal Twelfth Peoples Hospital
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2011

To analyze the changes in CD8(low) T lymphocyte subsets in patients with occupational chronic lead poisoning. Flow cytometric analysis was used to count the numbers of CD8+ cells. 23 patients with occupational chronic lead poisoning and 20 controls were examined. Compared with control group (8.21% ± 3.02%), the CD8(low) T lymphocyte (12.98% ± 5.62%) were significantly increased in patients with occupational chronic lead poisoning. Although the ratio of CD+ T lymphocyte is normal, the CD8 level is significantly decreased. The increase of CD8(low) T lymphocyte may be an important phenomenon of immuno-injury induced by lead. CD8(low) T lymphocyte could be an new direction for research of lead immuno-toxicity.

Liu W.W.,Guangzhou Municipal Twelfth Peoples Hospital
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2011

To evaluate the potential role of hepatocyte growth factor (HGF) combined with bone marrow mesenchymal stem cells (BMSC) autograft for the treatment of silicosis. Bone marrow (100 ml) was aspirated from a severe silicosis patient. BMSCs isolated, purified and cultured in vitro. When BMSC came to 70% confluence at passage 3, the culture medium was added liposomes (lipo2000) and plasmid-HGF (p-HGF) and cultured for 2 d. HGF-MSCSs (5 × 10(7) cells) were resuspended in 50 ml 0.9% sodium chloride (NS) and infused Intravenous drip at 3 consecutive times (once a week). Clinical follow-up were performed before and after treatment: (1) pulmonary high-kV X-ray, chest CT examination; (2) pulmonary function test; (3) determination of serum ceruloplasmin. The symptoms such as coughing, chest tightness disappeared at 12 months after treatment. Pulmonary function tests showed significant changes after treatment: forced vital capacity (FVC) increased from 64.6% to 81.0%, forced expiratory volume in one second (FEV(1.0)) increased from 68.7% to 90.1%, 1 second rate (FEV(1.0)/FVC%) reduced from 111.6% to 107.1%, the maximum mid-expiratory flow (FEF(25%∼75%) decreased from 100.2% to 94.6%, forced expiratory vital capacity 75% of the moment bit of gas flow (MEF(75%)) increased from 99.2% to 113.5%, forced expiratory vital capacity 50% of the moment bit of gas flow (MEF(50%)) increased from 125.3% to 130.2%, forced expiratory vital capacity 25% of the moment bit of gas flow (MEF(25%)) reduced from 86.9% to 71.7%; serum ceruloplasmin levels decreased from 690 mg/L to 180.6 mg/L; lung high-kV X-ray at 1st review showed that diffuse lung nodules had been absorbed and getting smaller than before treatment; chest CT showed that the distribution and number of small nodules at double lung fields decreased than before treatment. HGF combined with BMSC transplantation may have some potential role for the treatment of silicosis patients.

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