Guangzhou Institute of Respiratory Disease

Guangzhou, China

Guangzhou Institute of Respiratory Disease

Guangzhou, China
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Patent
Guangzhou University and Guangzhou Institute Of Respiratory Disease | Date: 2017-09-06

Provided are a method for extracting high-purity asarinin and use thereof, which belongs to the field of natural medicines. The method comprises: putting coarse asarum powder in a supercritical CO_(2) extraction device; under the conditions that an extraction kettle has a pressure of 30-40 Mpa and a temperature of 45-70C, separating kettles I and II have a pressure of 5-10 Mpa and a temperature of 30-50C and the flow rate of CO_(2) is 35-60 L/h, extracting for 60 to 240 minutes to obtain asarum total volatile oil; standing the asarum total volatile oil for 24-72 hours at a low temperature, and filtering under reduced pressure to obtain an asarinin coarse product; re-crystallizing the asarinin coarse product in absolute ethanol twice to obtain an asarinin pure product. In addition, it is found for the first time that asarinin has a good cough-relieving activity and thus can be used in preparing medicines for treating various types of coughs. Firstly disclosed is a brand-new preparation process for extracting asarinin by a supercritical CO_(2) extraction method, and this method has the advantages of simple process, no solvent residue, high product purity, etc.


Patent
Guangzhou University, Guangzhou Institute Of Respiratory Disease and State Key Laboratory Of Respiratory Disease | Date: 2017-03-22

An application of Fructus schisandrae total polysaccharides in the preparation of medicine or nutraceuticals used for treating coughing, and a pharmaceutical or nutraceutical composition containing Fructus schisandrae total polysaccharides. Experiments demonstrate that Fructus schisandrae total polysaccharides can be used for preparing antitussive and cough relief medicine and medicine to eliminate inflammation of the airways.


Patent
The First Affiliated Hospital Of Guangzhou Medical and Guangzhou Institute Of Respiratory Disease | Date: 2017-02-01

The present Invention discloses an Asarum total polysaccharide extract with antitussive activity, an extraction method thereon and an application in the preparation of a medicine for preventing and treating coughs. The present invention proves that the Asarum total polysaccharide extract has a significant effect of suppressing cough for the first time through a large number of pharmacodynamic tests, has the therapeutic effect approximate to codeine, has the effect of prolonging the cough latent period better than codeine, and also finds that the total Asarum polysaccharide extract has the effects of reducing the cough sensitivity and suppressing the airway inflammation for the first time, has excellent preventive and therapeutic effects on various types of coughs, and can be used for preparing the medicines for preventing and treating cough-related diseases.


Xiao R.,Guangzhou Panyu Central Hospital | Chen R.,Guangzhou Institute of Respiratory Disease
Molecular Medicine Reports | Year: 2017

Neutrophil gelatinase-associated lipocalin (NGAL) is a 25-kDa protein of the lipocalin superfamily and its presence was initially observed in activated neutrophils. It has previously been demonstrated that the expression of NGAL is markedly increased in stimulated epithelia, and is important in the innate immunological response to various pathophysiological conditions, including infection, cancer, inflammation and kidney injury. The present study constructed a ventilator-associated lung injury model in mice. NGAL mRNA and protein expression levels in lung tissue were detected using reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. In addition, NGAL protein levels in bronchoalveolar lavage fluid and serum were measured via western blotting. The results of the present study suggested that NGAL expression increased under all mechanical ventilation treatments. The increase was most prominent in the high peak inflation pressure and high-volume mechanical ventilation groups, where there was the greatest extent of lung injury. In addition, NGAL expression increased in a time-dependent manner under high-volume mechanical ventilation, consistent with the degree of lung injury. These findings suggested that NGAL may serve as a potential novel biomarker in ventilator-associated lung injury.


Quanjer P.H.,Erasmus Medical Center | Stanojevic S.,University College London | Stanojevic S.,Hospital for Sick Children | Cole T.J.,University College London | And 8 more authors.
European Respiratory Journal | Year: 2012

The aim of the Task Force was to derive continuous prediction equations and their lower limits of normal for spirometric indices, which are applicable globally. Over 160,000 data points from 72 centres in 33 countries were shared with the European Respiratory Society Global Lung Function Initiative. Eliminating data that could not be used (mostly missing ethnic group, some outliers) left 97,759 records of healthy nonsmokers (55.3% females) aged 2.5-95 yrs. Lung function data were collated and prediction equations derived using the LMS method, which allows simultaneous modelling of the mean (mu), the coefficient of variation (sigma) and skewness (lambda) of a distribution family. After discarding 23,572 records, mostly because they could not be combined with other ethnic or geographic groups, reference equations were derived for healthy individuals aged 3-95 yrs for Caucasians (n=57,395), African-Americans (n=3,545), and North (n=4,992) and South East Asians (n=8,255). Forced expiratory value in 1 s (FEV1) and forced vital capacity (FVC) between ethnic groups differed proportionally from that in Caucasians, such that FEV1/FVC remained virtually independent of ethnic group. For individuals not represented by these four groups, or of mixed ethnic origins, a composite equation taken as the average of the above equations is provided to facilitate interpretation until a more appropriate solution is developed. Spirometric prediction equations for the 3-95-age range are now available that include appropriate age-dependent lower limits of normal. They can be applied globally to different ethnic groups. Additional data from the Indian subcontinent and Arabic, Polynesian and Latin American countries, as well as Africa will further improve these equations in the future. Copyright©ERS 2012.


Caoa C.,The Systematic Review Unit | Zhub Z.-H.,Sun Yat Sen University | Yana T.D.,University of Sydney | Wang Q.,Fudan University | And 10 more authors.
European Journal of Cardio-thoracic Surgery | Year: 2013

Objectives: Comparative long-term survival and oncological outcomes for patients with non-small-cell lung cancer (NSCLC) who undergo video-assisted thoracic surgery (VATS) or conventional open lobectomy remain uncertain. We conducted a multi-institutional propensity-matched study to stratify potential differences in these outcomes. Methods: We established a multi-institutional registry for 4312 patients with NSCLC who underwent lobectomy between 2001 and 2008 from eight institutions in the People's Republic of China. Age, gender, histological type and tumour staging were entered into a nonparsimonious multivariable logistic regression model to assess long-term survival outcomes. The predicted probability derived from the logistic equation was used as the propensity score for each individual. Based on similar propensity scores, we matched 1458 of the 1700 patients who underwent VATS lobectomy with 1458 of the 2612 patients who underwent open lobectomy and compared their long-term survival outcomes. Results: The mean age of the 2916 matched patients was 59 (standard deviation = 11) years. After propensity-matching, VATS and open lobectomy patients were similar in regards to important prognostic variables. Three prognostic factors were independently associated with improved survival in the multivariate analysis: age < 60 (P < 0.001), female gender (P = 0.013) and pathological staging (P < 0.001). Patients who underwent VATS vs open lobectomy had similar long-term survival (P = 0.07). Conclusions: The current propensity score analysis suggests that well-matched patients with NSCLC who underwent standardized VATS lobectomy had similar long-term survival outcomes when compared with those who underwent open lobectomy. © The Author 2013. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.


De Miranda N.F.C.C.,Karolinska University Hospital | Georgiou K.,Karolinska University Hospital | Chen L.,CAS Beijing Institute of Genomics | Wu C.,Karolinska University Hospital | And 10 more authors.
Blood | Year: 2014

Next-generation sequencing studies on diffuse large B-cell lymphomas (DLBCLs) have revealed novel targets of genetic aberrations but also high intercohort heterogeneity. Previous studies have suggested that the prevalence of disease subgroups and cytogenetic profiles differ between Western and Asian patients. To characterize the coding genome of Chinese DLBCL, we performed whole-exome sequencing of DNA derived from 31 tumors and respective peripheral blood samples. The mutation prevalence of B2M, CD70, DTX1, LYN, TMSB4X, and UBE2A was investigated in an additional 105 tumor samples. We discovered 11 novel targets of recurrent mutations in DLBCL that included functionally relevant genes such as LYN and TMSB4X. Additional genes were found mutated at high frequency (≥10%) in the Chinese cohort including DTX1, which was the most prevalent mutation target in the Notch pathway. We furthermore demonstrated that mutations in DTX1 impair its function as a negative regulator of Notch. Novel and previous unappreciated targets of somatic mutations in DLBCL identified in this study support the existence of additional/alternative tumorigenic pathways in these tumors. The observed differences with previous reports might be explained by the genetic heterogeneity of DLBCL, the germline genetic makeup of Chinese individuals, and/or exposure to distinct etiological agents. © 2014 by The American Society of Hematology.


Patent
Guangzhou University and Guangzhou Institute Of Respiratory Disease | Date: 2015-03-25

The present invention discloses an Asarum total polysaccharide extract with antitussive activity, an extraction method thereof and an application in the preparation of a medicine for preventing and treating coughs. The present invention proves that the Asarum total polysaccharide extract has a significant effect of suppressing cough for the first time through a large number of pharmacodynamic tests, has the therapeutic effect approximate to codeine, has the effect of prolonging the cough latent period better than codeine, and also finds that the total Asarum polysaccharide extract has the effects of reducing the cough sensitivity and suppressing the airway inflammation for the first time, has excellent preventive and therapeutic effects on various types of coughs, and can be used for preparing the medicines for preventing and treating cough-related diseases.


Zhou W.,Chinese Academy of Sciences | Hu W.,Chinese Academy of Sciences | Hu W.,Guangzhou Institute of Respiratory Disease
Future Medicinal Chemistry | Year: 2013

Alzheimer's disease (AD) is complicated and difficult to fully understand, it might need multiple drug-discovery strategies to combat the disease. Regardless of the cause of AD, neuronal death in the brain plays a key role in AD progression and is directly linked to neuroinflammation. Thus, the regulation of neuroinflammatory processes might be a practical strategy for the treatment of AD. This review highlights the development of anti-neuroinflammatory agents that have shown promise in vitro or in vivo by attenuating microglial activation or cognitive decline. The agents are categorized based on the related signaling pathways, including the receptor for advanced glycation end products, p38 MAPKs, NF-κB and peroxisome proliferator-activated receptor γ and inhibitors against microglial activation lacking clear mechanisms. These anti-neuroinflammatory agents support the concept and represent important chemical probes for the development of anti-neuroinflammatory drugs for the treatment of AD. © 2013 Future Science Ltd.


Li H.,Guangzhou Institute of Respiratory Disease
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2013

To study the different expressions of glypican-3 in lung squamous cell carcinoma and adenocarcinoma and explore the association of glypican-3 with the prognosis of the patients. Glypican-3 expression was detected immunohistochemically in the tumor tissues and adjacent tissues from 48 cases of lung squamous cell carcinoma and adenocarcinoma. Kaplan-Meier method and log-rank test were used for survival analysis of the patients. Glypican-3 expression was detected in the tumor tissues in 29.2% (14/48) of the cases, but not in the adjacent tissues. Of the 22 patients with lung squamous cell carcinoma, 12 (54.5%) showed positive glypican-3 expression in the tumor tissue, a rate significantly higher than that in patients with lung adenocarcinoma [7.7% (2/26), P<0.01]. In all the glypican-3-positive cases, the tumor tissues showed stronger glypican-3 expression in cases with lymph node metastasis or poor tumor differentiation. Kaplan-Meier survival analysis did not indicate a significant correlation of glypican-3 expression with the prognosis of the lung cancer patients. Patients with lung squamous cell carcinoma have higher glypican-3 expressions in the tumor tissues than those with lung adenocarcinoma, suggesting the value of glypican-3 protein as a potential marker to detect lung squamous cell carcinoma.

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