Zhang X.,Forensic Science Institute of Guangzhou |
Sun L.,Forensic Science Institute of Guangzhou |
Liu J.,Forensic Science Institute of Guangzhou |
Xu S.,Forensic Science Institute of Guangzhou
Chinese Journal of Forensic Medicine | Year: 2011
Objective To establish a solid phase extraction (SPE)/UPLC method for the determination of morphine, amphetamines and ketamine in whole blood simultaneously. Methods Morphine, MA, MDMA, MDA, ketamine were extracted from whole blood using Agilent SCX 3cc (60mg) extraction cartridges and detected by UPLC-PDA. Qualitative and quantitative analysis was obtained by retention time and UV - spectrum. Results The recoveries for morphine, MA, MDMA, MDA, ketamine were 81.4% ± 2.51%, 88.2% ±2.48%, 91.8% ± 2.03%, 93.8% ± 1.46%, 74.8% ± 2.27% respectively, The correlation coefficient of linear calibration curve was over 0.999 within concentration range 0.08 ~ 100μg/mL, 0.4 ~ 100 μg/mL, 0.2 ~75μg/mL, 0.3 ~75μg/mL, 0.4 ~ 100μg/mL respectively. The limits of qualification were 30pg, 200pg, 80pg, 100pg, 200pg respectively. Conclusion The method was simple, rapid and accurate for qualitative and quantitative analysis of morphine, amphetamines, ketamine in whole blood simultaneously.