Guangzhou Institute for Drug Control

Guangzhou, China

Guangzhou Institute for Drug Control

Guangzhou, China

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Peng Y.,Sun Yat Sen University | Chen X.,Sun Yat Sen University | Liu Q.,Southern Medical University | Zhang X.,Sun Yat Sen University | And 12 more authors.
Leukemia | Year: 2015

Refractory chronic graft-versus-host disease (cGVHD) is a significant complication resulting from allogeneic hematopoietic stem cell transplantation (HSCT). Mesenchymal stromal cells (MSCs) have shown promise for treating refractory cGVHD, but the favorable effects of MSCs therapy in cGVHD are complex and not fully understood. In this prospective clinical study, 20 of 23 cGVHD patients had a complete response or partial response in a 12-month follow-up study. The most marked improvements in cGVHD symptoms were observed in the skin, oral mucosa and liver. Clinical improvement was accompanied by a significantly increased number of interleukin (IL)-10-producing CD5+ B cells. Importantly, CD5+ B cells from cGVHD patients showed increased IL-10 expression after MSCs treatment, which was associated with reduced inflammatory cytokine production by T cells. Mechanistically, MSCs could promote the survival and proliferation of CD5+ regulatory B cells (Bregs), and indoleamine 2, 3-dioxygenase partially participates in the MSC-mediated effects on Breg cells. Thus, CD5+ Breg cells may have an important role in the process of MSC-induced amelioration of refractory cGVHD and may provide new clues to reveal novel mechanisms of action for MSCs. © 2015 Macmillan Publishers Limited. All rights reserved.


Yan X.-H.,Guangzhou Institute for Drug Control | Liu H.-L.,CAS Shanghai Institute of Materia Medica | Huang H.,CAS South China Sea Institute of Oceanology | Li X.-B.,CAS South China Sea Institute of Oceanology | Guo Y.-W.,CAS Shanghai Institute of Materia Medica
Journal of Natural Products | Year: 2011

Eight new marine steroids, characterized by either the presence of an aromatic ring or a cross-conjugated dienone system in ring A, were isolated from the Hainan soft coral Dendronephthya studeri Ridley. Their structures were elucidated on the basis of detailed spectroscopic analysis and by comparison of their NMR data with those reported in the literature. © 2010 The American Chemical Society and American Society of Pharmacognosy.


Fan J.-P.,Nanchang University | Cao J.,Nanchang University | Zhang X.-H.,Nanchang University | Huang J.-Z.,Guangzhou Institute for Drug Control | And 6 more authors.
Food Chemistry | Year: 2012

Ionic liquid (IL) based ultrasonic assisted extraction (ILUAE) was developed for the effective extraction of puerarin from Radix Puerariae Lobatae (RPL). The ILUAE parameters including the type of ILs, IL concentration, RPL amount, ultrasonic power and time were optimized by single-factor experiment and response surface methodology. Under the optimized experimental conditions, the best results were obtained using RPL amount 0.43 g in 10 mL 1.06 mol L -1 1-butyl-3-methylimidazolium bromide aqueous solution, ultrasonic time 27.43 min and ultrasonic power 480 W. Scanning electron microscope images of RPL samples were obtained to provide visual evidence of the sonication effect. Compared with the conventional ultrasonic assisted extraction and refluent extraction, the proposed ILUAE offered shorter extraction time and remarkable higher efficiencies due to the higher penetration ability and solubility of IL and the cavitation phenomenon produced in the solvent by the passage of an ultrasonic wave, which further supported the suitability of the proposed approach. © 2012 Elsevier Ltd. All rights reserved.


Fan J.-P.,Nanchang University | Zhang L.,Nanchang University | Zhang X.-H.,Nanchang University | Huang J.-Z.,Guangzhou Institute for Drug Control | And 4 more authors.
Analytical and Bioanalytical Chemistry | Year: 2012

In this work, molecularly imprinted solid-phase extraction (MISPE) has been used to selectively enrich, purify, or remove synephrine from Aurantii Fructus Immaturus. To this end, a molecularly imprinted polymer (MIP) was prepared by self-assembly from the template synephrine, the functional monomer methacrylic acid, and the crosslinker ethylene glycol dimethacrylate in 1:4:20 molar ratio. Subsequent molecular interrogation of the MIP binding sites revealed preferential structural selectivity for synephrine relative to other structurally related naturally occurring compounds (i.e. octopamine and tyramine ). This selectivity was subsequently exploited to achieve substantial sample clean-up of extracts of crude Aurantii Fructus Immaturus and Aurantii Fructus Immaturus stir-baked with bran. The purity of synephrine in the extracts after MISPE represented approximately 24.21-fold enrichment of the synephrine in the untreated extracts of Aurantii Fructus Immaturus stir-baked with bran. High recoveries (85-90%) from the samples proved that the method was valid for selective enrichment, purification, or removal of synephrine from Aurantii Fructus Immaturus. © 2011 Springer-Verlag.


Hu Y.M.,Chinese University of Hong Kong | Su G.H.,Guangzhou Institute for Drug Control | Sze S.C.-W.,Chinese University of Hong Kong | Ye W.,China Pharmaceutical University | Tong Y.,Chinese University of Hong Kong
Biomedical Chromatography | Year: 2010

A simple method based on liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI-MS) was developed for the quality assessment of Cortex Phellodendri (CP), which was mainly derived from two species of Phellodendron chinense Schneid and Phellodendron amurense Rupr. Total 41 compounds, including 14 phenols, 24 alkaloids and three liminoidal triterpenes were identified or tentatively characterized from the 75% methanol extract of CP samples by online ESI-MS n fragmentation and UV spectra analysis. Among them, two phenols and six alkaloids were simultaneously quantified using HPLC-DAD method. The validated HPLC-DAD method showed a good linearity, precision, repeatability and accuracy for the quantification of eight marker compounds. Furthermore, the plausible fragmentation pathway of the representative compounds were proposed in the present study. The differences of the chemical constituents content and the comprehensive HPLC profiles between the two CP species using LC-DAD-ESI-MS method are reported for the first time, indicating that the CP drugs from different resources should be used separately in the clinic. Copyright © 2009 John Wiley & Sons, Ltd.


Qi P.,South China University of Technology | Qi P.,Guangzhou Institute for Drug Control | Lin Z.,Guangzhou Institute for Drug Control | Li J.,Guangzhou Institute for Drug Control | And 4 more authors.
Food Chemistry | Year: 2014

In this work, a simple, rapid and sensitive analytical method for the determination of rhodamine B in chili-containing foodstuffs is described. The dye is extracted from samples with methanol and analysed without further cleanup procedure by high-performance liquid chromatography (HPLC) coupled to fluorescence detection (FLD). The influence of matrix fluorescent compounds (capsaicin and dihydrocapsaicin) on the analysis was overcome by the optimisation of mobile-phase composition. The limit of determination (LOD) and limit of quantification (LOQ) were 3.7 and 10 μg/kg, respectively. Validation data show a good repeatability and within-lab reproducibility with relative standard deviations <10%. The overall recoveries are in the range of 98-103% in chili powder and in the range of 87-100% in chili oil depending on the concentration of rhodamine B in foodstuffs. This method is suitable for the routine analysis of rhodamine B due to its sensitivity, simplicity, reasonable time and cost. © 2014 Elsevier Ltd. All rights reserved.


Yan X.H.,Guangzhou Institute for Drug Control
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To study the contents of cadmium in Cortex Ilicis Rotundae and provide evidence for establishing the detection limit of cadmium in its quality standard. ICP-MS was used to determine the contents of cadmium in 18 batches of Ilex L. The contents of cadmium in 10 batches of Cortex Ilex Rotunda ranged from 3.4 to 10.4 mg/kg,and the contents of cadmium in Ilex asprella and Ilex pubescents were more than 0.3 mg/kg. The contents of cadmium of Cortex Ilicis Rotundae, Ilex asprella and Ilex pubescents have particular characterise, and this study provides a basic for the analysis of cadmium in Cortex Ilicis Rotundace and other plants of this genus.


Bi F.-J.,Guangzhou Institute for Drug Control | Lin T.,Guangzhou Institute for Drug Control
Chinese Traditional and Herbal Drugs | Year: 2016

Objective: To establish HPLC method for the simultaneous determination of protopine, palmatine hydrochloride, berberine hydrochloride, dehydrocorydaline, tetrahydropalmatine, tetrahydroberberine, and corydaline in Cuyanhusuo Granule. Methods: The analysis was performed on Ultimate AQ-C18 column (250 mm×4.6 mm, 5 μm) by gradient elution of acetonitrile-0.1% phosphoric acid (adjust to pH 6.0 with triethylamine) (10:90). The UV detection wavelength was set at 280 nm and the flow rate was 1.0 mL/min. Results: The linear ranges of protopine, palmatine hydrochloride, berberine hydrochloride, dehydrocorydaline, tetrahydropalmatine, tetrahydroberberine, and corydaline were 6.8-119.0 (r=0.9999), 24.38-426.65 (r=0.9999), 8.88-155.40 (r=0.9999), 77.66-1359.05 (r=0.9999), 41.4-724.5 (r=0.9999), 6.70-117.25 (r=0.9999), and 25.50-446.25 ng (r=0.9999). The average recoveries (n=6) were 98.2% (RSD=2.0%), 99.6% (RSD=2.8%), 100.2% (RSD=1.3%), 99.0% (RSD=2.2%), 100.8% (RSD=2.6%), 98.7% (RSD=2.5%), and 97.7% (RSD=2.2%), respectively. Conclusion: This method is simple and rapid, and can be used for the quality control of Cuyanhusuo Granule with satisfactory separation and repeatability. © 2016, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.


Hou J.,South China Agricultural University | Hou J.,Guangzhou Institute for Drug Control | Yang X.,South China Agricultural University | Zeng Z.,South China Agricultural University | And 4 more authors.
Journal of Antimicrobial Chemotherapy | Year: 2013

Objectives: To investigate the occurrence of plasmid-mediated fosfomycin resistance genes among Escherichia coli from food animals in China. Methods: A total of 892 E. coli isolates collected from individual pigs (n=368), chickens (n=196), ducks (n=261), geese (n=35), pigeons (n=20) and partridges (n=12) in Guangdong Province during 2002-08 were screened for the presence of fosA3, fosA and fosC2 by PCR amplification and sequencing. The clonal relationship of fosA3-positive isolates, plasmid content and other associated resistance genes were also characterized. Results: Twelve (1.3%) E. coli isolates showed resistance to fosfomycin and 10 (1.1%) isolates (4 from pigs, 2 from chickens, 2 from ducks, 1 from a goose and 1 from a pigeon) were positive for fosA3. None of the E. coli isolates was positive for fosA or fosC2. All of the isolates carrying fosA3 were CTX-M producers, and three of them carried rmtB. Most of the fosA3-harbouring isolates were found to be clonally unrelated. The fosA3 genes were flanked by IS26. Two fosA3 genes co-localized with rmtB and blaCTX-M-65 on indistinguishable F33:A-:B- plasmids that carried three addiction systems (pemI/pemK, hok/mok/sok and srnB). Four, one and one fosA3 genes were found to be associated with IncN (ST8 type), IncI1 and F2:A-:B- plasmids, respectively. Conclusions: We discovered that fosA3 is always associated with blaCTX-M, which facilitates its quick dispersal. The emergence of fosA3 in food animals could impact on human medicine by the potential transfer of resistance through the food chain. © The Author 2012. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.


Jin G.-Y.,Guangzhou Institute for Drug Control | Li H.,Guangzhou Institute for Drug Control | Xu W.-B.,Guangzhou Institute for Drug Control
Journal of Pharmaceutical Analysis | Year: 2012

Glassy carbon electrode, which is used to electrochemically determine the content of buformin, is modified with an electropolymerized film of p-aminobenzoic acid in pH 7.0 acetate buffer solution (ABS). The polymer showed an excellent electrocatalytic activity for the reduction of buformin. In pH 7.0 ABS, the cathodic peak current increased linearly over three concentration intervals of buformin, and the detection limit (S/N=3) was 2.0×10 -9 g/mL. The method was successfully applied to directly determine buformin in tablets with standard addition recoveries of 95.8-102.5%. The proposed method is simple, cheap and highly efficient. © 2012 Xi'an Jiaotong University.

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