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Guo J.W.,Guangzhou Hospital of Traditional Chinese Medicine
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To research the effects of Panax notoginseng saponins (PNS) on angiotensin-converting enzymes 2 ( ACE2) and tumor necrosis factor-alpha (TNF-alpha) in rats with post-myocardial infarction ventricular remodeling. Models of acute myocardial infarction (AMI) were produced by ligation of left anterior descending coronary artery, 24 hours after operation the rats were randomly divided into control and experiment groups, then respectively administrated with NS, fosinopril and low, middle and high dosage of PNS for four consecutive weeks. To observe effects of PNS on malondialdehyde (MDA), nitric oxide (NO), glutathione peroxidase (GSH-Px), ACE2 and TNF-alpha in rats with post-myocardial infarction ventricular remodeling. Compared with NS group, MDA significantly decreased, the activity of GSH-Px significantly increased (P < 0.05 or P < 0.01), NO of the high-dose PNS group decreased (P < 0.05), Compared with the NS group, ACE2 increased and TNF-a significantly decreased in low-dose PNS group, middle and high-dose groups (P < 0.05). PNS can stimulate ACE2 to inhibit the expression of TNF-alpha and enhance the antioxidance. PNS can reduce pathological injury of cardiac myocytes in myocardial ischemia and cardiac muscle, which can improve ventricular remodeling. Source


Ye X.,Guangzhou University of Chinese Medicine | Lu D.,Guangzhou University of Chinese Medicine | Chen X.,Guangzhou University of Chinese Medicine | Li S.,Guangzhou University of Chinese Medicine | And 2 more authors.
Journal of Pain and Symptom Management | Year: 2016

Context Shuangbai San is a Chinese herb preparation used externally to treat pain. There have been few randomized controlled trials addressing the safety and usefulness of Shuangbai San, such as its effect on pain relief and quality of life (QOL) improvement. Objectives This study was conducted to evaluate the effect of Shuangbai San on relieving pain and improving QOL in primary liver cancer patients with cancer pain. Methods A total of 134 primary liver cancer patients with mild pain (numerical rating scale [NRS] ≤ 3), either locally in the liver or in the upper abdomen, were enrolled and randomly allocated to the group receiving Shuangbai San or the control group (receiving placebo). The primary outcome measures were the NRS score and QOL scales, including the QOL scale for patients with liver cancer, version 2.0 and the European Organization for Research and Treatment of Cancer QOL Questionnaire-C30. The secondary outcome measures included the Karnofsky Performance Status score, blood indicators, and liver and kidney function before and after treatment. Results The NRS scores decreased more significantly in the Shuangbai San group than in the placebo group (P < 0.05) at the corresponding time points. The changes in the scores for the physical function, psychological function, and symptoms/adverse effects domains of the QOL scale for patients with liver cancer, version 2.0 and the physical, emotional, and cognitive domains of the European Organization for Research and Treatment of Cancer QOL Questionnaire-C30 were significantly greater in the Shuangbai San group than in the placebo group (P < 0.05). The changes in the scores for the other domains were not significantly different (P > 0.05). Conclusion The use of Shuangbai San can relieve mild pain in liver cancer patients and improve their QOL. © 2016 American Academy of Hospice and Palliative Medicine. Source


Huang D.,Guangzhou Hospital of Traditional Chinese Medicine | Liu M.,Guangzhou Institute of Traditional Chinese Medicine | Yan X.,Guangzhou Institute of Traditional Chinese Medicine
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To observe the effects of hippocampal Aβ42 deposition on the expression of inflammatory cytokines and phosphorylated MAPK signal molecules as well as the intervention of AD by total glucosides of paeony(TGP). Method: 12 week-old female SD rats were stereotactic injected one-time with a fibrillar Aβ42 positioning hippocampus to replicate AD pathology model and interfered with TGP. The expression of inflammatory cytokines and phosphorylated MAPK pathway signaling molecules were observed by immunohistochemistry (SABC), and SABC images were analyzed by image analysis software. Result: Compared with the control group, the IL-1β, IL-6 and p-p38, p-JNK, p-MEK3/6 positive stained areas of AD pathology model group increased and their staining intensity decreased (the protein expression quantity inversely proportional to the staining intensity), while the IL-1β, IL-6 and p-p38, p-JNK, p-MEK3/6 positive stained areas of the treatment groups decreased and their staining intensity increased compared with AD pathology model group. Conclusion: Aβ42 deposition in hippocampus can induce the brain inflammation and the over-expression of IL-1β, IL-6 and p-p38, p-JNK, p-MEK3/6. Inhibiting the over-expression of inflammatory cytokines and phosphorylated MAPK signaling molecules may be a major antagonistic mechanism of TGP against AD. Source


Huang D.,Guangzhou Hospital of Traditional Chinese Medicine | Xiao H.,Guangzhou Medical College | Qiu F.,Guangzhou Medical College | Nie J.,Southern Medical University
Molecular Medicine Reports | Year: 2013

Tyrosine phosphorylated proteins govern a host of cell functions, such as growth, division, adhesion and motility. We previously identified a group of Nck Src homology 2 (SH2) domain-binding proteins by combining the GST-Nck1-SH2 pull-down method with two-dimensional electrophoresis (2-DE) in hepatocellular carcinoma (HCC) tissues. In the present study, different methods and conditions for key procedures of GST-Nck1-SH2 pull-down and 2-DE were investigated and optimized. High-resolution results were obtained using the following conditions: a total amount of 100 μl GST-Nck1-SH2 fusion proteins/10 mg liver proteins to execute the pull-down procedure; 7 M urea and 2 M thiourea as lysis buffer; ultra-filtration depletion of interferential materials. Moreover, we performed a negative control experiment using GST-4T3 during the pull-down procedure, and further demonstrated that the proteins obtained using the aforementioned method interacted with Nck in a tyrosine phosphorylation-dependent manner. The optimized method offers a rapid, efficient alternative for the high-quantity screening of tyrosine-phosphorylated protein expression and solubility, which in turn facilitates future studies on tyrosine-phosphorylated proteins. Copyright © 2013 Spandidos Publications Ltd. Source


Zeng Y.-T.,Guangdong Academy of Medical science | Jiang J.-M.,Sun Yat Sen University | Lao H.-Y.,Guangdong Academy of Medical science | Guo J.-W.,Guangzhou Hospital of Traditional Chinese Medicine | And 2 more authors.
Molecular Medicine Reports | Year: 2015

Cancer is one of the most eminent diseases of modern times and numerous natural products derived from medicinal plants have been identified as potential sources of antitumor drugs. A successful anticancer drug must target or inhibit tumor cells whilst causing minimal damage to healthy cells. The present study aimed to investigate the antitumor efficacy of ethyl acetate extract, and other isolated compounds from Artemisia indica, on MCF-7, BHY, Miapaca-2, Colo-205 and A-549 cell lines. The apoptotic activity of the compounds was studied using flow cytometry. The different cancer cell lines were treated with the ethyl acetate extract and varying concentrations of compounds (denoted a-g) isolated from the A. indica. The cytotoxicity was evaluated by MTT assay and the apoptotic properties of the compounds and the extract were assessed using flow cytometry. In MCF-7 cells, the effect on mitochondrial membrane potential loss (λαm) induced by compounds b and d was also studied. Bioassay-guided fractionation of the ethyl acetate extract from the shoot and root parts of A. indica led to the identification of the compounds a-g as: 5-hydroxy-3,7,4'-trimethoxyflavone; ludartin; maackiain; lupeol; cis-matricaria ester; trans-matricaria ester; and 6-methoxy-7,8-methylenedioxy coumarin, respectively. All the compounds exhibited mild to potent inhibition of cell proliferation in all the cell lines, with the half maximal inhibitory concentration values ranging from 25.18-88.12 μM. Ludartin and lupeol were observed to have the most potent inhibitory effects. Based on the initially identified antiproliferative effects, these two compounds were evaluated for their effects on cell cycle phase distribution, DNA damage and their effects on mitochondrial membrane potential loss (λαm). The two compounds induced DNA damage and mitochondrial membrane potential loss in MCF-7 cells. The results of the current study suggest that lupeol and ludartin, isolated from A. indica, produce anticancer effects by inducing DNA damage and a reduction of mitochondrial membrane potential, and may be used as potent anticancer agents, subsequent to further study. Source

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