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Xu F.-T.,Gannan Medical University | Li H.-M.,Guangxi Medical University | Yin Q.-S.,Guangzhou General Hospital of PLA | Liu D.-L.,Southern Medical University | And 3 more authors.
Cellular Physiology and Biochemistry | Year: 2014

Background: The main complication of autologous free fat tissue transplantation is fat resorption and calcification due to the ischemic necrosis of fat. The promotion of transplant neovascularization soon after autologous free fat grafts may reduce these outcomes. In adulthood, stromal cell-derived factor-1 (SDF-1) and its membrane receptor C-X-C chemokine receptor type 4 (CXCR4) are involved in the homing and migration of multiple stem cell types, neovascularization, and cell proliferation. We hypothesized that CXCR4 may improve the long-term survival of free fat tissue transplants by recruiting endothelial progenitor cells (EPCs) and may therefore improve graft revascularization. In this study, we aimed to determine the effect of human breast adipose-derived stem cells (HBASCs) transfected with the CXCR4 gene on the survival rate of human autologous free fat transplants in nude mice. Methods: Human breast adipose-derived stem cells (HBASCs) were expanded ex vivo for 3 passages, labeled with green fluorescent protein (GFP) and transfected with CXCR4 or left untransfected. Autologous fat tissues were mixed with the GFP-labeled, CXCR4-transfected HBASCs (group A), GFP-labeled HBASCs (group B), the known vascularization-promoting agent VEGF (group C), or medium (group D) and then injected subcutaneously into 32 nude mice at 4 spots in a random fashion. Six months later, the transplanted tissue volume and histology were evaluated, and neo-vascularization was quantified by counting the capillaries. CXCR4 and SDF-1α mRNA expression in the transplants was determined using real-time quantitative PCR analysis (qPCR). Results: The data revealed that the control (group D) transplant volume survival was 28.3 ± 4.5%. Mixing CXCR4-transfected (group A) and untransfected (group B) HBASCs significantly increased transplant volume survival (79.5 ± 8.3% and 67.2 ± 5.9%, respectively), whereas VEGF-transfected HBASCs (group C) were less effective (41.2 ± 5.1%). Histological analysis revealed that both types of HBASCs-treated transplants consisted predominantly of adipose tissue, unlike the control transplants, and also presented significantly less fat necrosis and fibrosis. The CXCR4-transfected HBASCs-treated transplants had a significantly higher capillary density than did the other transplants and showed GFP and CD31 double-positive cells (i.e., ASCs-derived endothelial cells). The mRNA expression of CXCR4 and SDF-1α was much higher in the CXCR4-transfected HBASCs transplants than in the other three transplants. Conclusions: Our data demonstrated that HBASCs can enhance the survival and quality of transplanted free fat tissues. Moreover, CXCR4 transfection of these HBASCs could augment this effect. Stimulation of angiogenesis and decreased fat cell apoptosis due to the recruitment of endothelial progenitor cells (EPCs) and an increase in graft revascularization are potential mechanisms underlying the improved long-term survival of free fat transplants following CXCR4-transfected HBASCs treatment. © 2015 S. Karger AG, Basel.


Xu F.-T.,Gannan Medical University | Li H.-M.,Guangxi Medical University | Yin Q.-S.,Guangzhou General Hospital of PLA | Liang Z.-J.,Guangxi Medical University | And 5 more authors.
American Journal of Translational Research | Year: 2015

To investigate whether activated autologous platelet-rich plasma (PRP) can promote proliferation and osteogenic differentiation of human adipose-derived stem cells (hASCs) in vitro. hASCs were isolated from lipo-aspirates, and characterized by specific cell markers and multilineage differentiation capacity after culturing to the 3 rd passage. PRP was collected and activated from human peripheral blood of the same patient. Cultured hASCs were treated with normal osteogenic inductive media alone (group A, control) or osteogenic inductive media plus 5%, 10%, 20%, 40%PRP (group B, C, D, E, respectively). Cell proliferation was assessed by CCK-8 assay. mRNA expression of osteogenic marker genes including alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN) and core binding factor alpha 1 (Cbfa1) were determined by Real-Time Quantitative PCR Analysis (qPCR). Data revealed that different concentrations of activated autologous PRP significantly promoted hASCs growth in the proliferation phase compared to the without PRP group and resulted in a dose-response relationship. At 7-d and 14-d time point of the osteogenic induced stage, ALP activity in PRP groups gradually increased with the increasing of concentrations of PRP and showed that dose-response relationship. At 21-d time point of the osteogenic induced stage, PRP groups make much more mineralization and mRNA relative expression of ALP, OPN, OCN and Cbfa1 than that without PRP groups and show that dose-response relationship. This study indicated that different concentrations of activated autologous PRP can promote cell proliferation at earlier stage and promote osteogenic differentiation at later stage of hASCs in vitro. Moreover, it displayed a dose-dependent effect of activated autologous PRP on cell proliferation and osteogenic differentiation of hASCs in vitro. © 2015, E-Century Publishing Corporation. All right reserved.


PubMed | Guangzhou General Hospital Of Pla, Community Health Service Center, Shanghai Medical Science & Technology Information Center, Fudan University and 3 more.
Type: Journal Article | Journal: BMC geriatrics | Year: 2016

Both multimorbidity and activities of daily living (ADL) disability and instrument activities of daily living (IADL) disability are common among elderly individuals. ADL/IADL disability may reduce individuals capacities for independent living and quality of life. This study aimed to examine the association between multimorbidity and ADL/IADL disability.A multi-stage cluster sample of 2058 residents aged 80 or older was investigated in Shanghai, China. Multimorbidity was defined as the simultaneous presence of two or more chronic diseases with ten common chronic conditions under consideration. Subjects who responded that they need partial or full assistance to any ADL/IADL items were defined as having ADL/IADL disability. We examined the association of multimorbidity with ADL/IADL disability, adjusted for socio-demographic characteristics by using logistic regression.Of respondents, 23.23% had ADL disability, 37.90% had IADL disability, and 49.17% had multimorbidity. After adjusted socio-demographic characteristics, a graded association was showed between ADL disability and the quantity of chronic conditions: odds ratio (OR) for 1 condition, 1.53(95% confidence interval [CI], 1.04-2.24); OR for 2 conditions, 2.06(95% CI, 1.43-2.96); OR for 3 conditions, 3.23(95% CI, 2.14-4.86); OR for 4 or more conditions, 5.61(95% CI, 3.26-9.66). Similar associations were also observed between the quantity of chronic conditions and IADL disability.The quantity of chronic conditions had relatively strong association with both ADL and IADL disability. Initiating prevention of additional chronic conditions and interventions on clusters of diseases may decrease the potential risk of ADL/IADL disability. Additionally, more attention should been given to the older low-income women living with relatives/non-relatives with multimorbidity.


Chen L.,Chinese PLA General Hospital | Li L.,Guangzhou General Hospital of PLA | Wang Y.,Fuzhou General Hospital of Nanjing Command | Li P.,Chinese PLA General Hospital | And 4 more authors.
Cancer Causes and Control | Year: 2013

Purpose: C-peptide, a hormone secreted by the pancreas, is a marker for insulin production and hyperinsulinemia. Epidemiological studies have suggested an association between circulating C-peptide level and colorectal neoplasia risk; however, the results were not always consistent. Herein, we conducted a systematic review and meta-analysis study to evaluate the association between circulating C-peptide level and the colorectal neoplasia risk. Methods: The PubMed database was searched for the eligibility studies updated to May 2013, which prospectively evaluated the association between circulating C-peptide level and colorectal neoplasia risk. The summary estimates and 95 % confidential intervals (95 % CIs) for those with the highest quantile C-peptide level in contrast to the lowest quantile were estimated with the random-effects model. Heterogeneity between the studies was assessed with the Q test and the I 2 statistic. Potential publication bias was evaluated with the Egger's test. Results: We identified 9 nested case-control studies that have recruited a total of 3,109 cases and 4,285 controls met the criteria. From the meta-analysis, we found that subjects with high circulating C-peptide were associated with a 37 % increased colorectal neoplasia risk [pooled odds ratios (OR) 1.37, 95 % CI 1.09-1.72] under the random-effects model. In the stratification studies, we found the association was more prominent in the men (pooled OR 2.34, 95 % CI 1.36-4.04) compared to women (pooled OR 1.41, 95 % CI 0.89-2.25). Significant association between circulating C-peptide level and colon cancer risk was found (pooled OR 1.72, 95 % CI 1.26-2.36), but not for rectal cancer (pooled OR 1.14, 95 % CI 0.75-1.73). No significant publication bias was found for any meta-analysis study. Conclusion: In conclusion, the results of the meta-analysis studies suggested that higher circulating C-peptide could be a predictive factor for higher colorectal neoplasia susceptibility. © 2013 Springer Science+Business Media Dordrecht.


Du Z.,Southern Medical University | Du Z.,Key Laboratory for Organ Failure Research | Zeng Q.,Southern Medical University | Zeng Q.,Key Laboratory for Organ Failure Research | And 8 more authors.
Experimental and Clinical Cardiology | Year: 2014

Background/Objective: Metabolomics studies have demonstrated that the serum and urine metabolic profiles of patients with chronic heart failure are different from those of patients with normal heart function. However, it is difficult to eliminate irrelevant factors, such as metabolic diseases, diet, activity status and other factors that can affect patient metabolic profiles. Therefore, we used a rat model with coronary artery ligation to mimic the pathogenesis of ischemic heart failure and subsequent chromatography/mass spectroscopy (GC/MS) analysis to determine whether the observed differences in serum metabolic profiles were caused by heart failure. Methods: Fifty-one male SD rats (SPF) were randomized into the sham group and the surgery group. The rat model of chronic ischemic heart failure was prepared by coronary artery ligation. Venous blood from the inferior vena cava was collected after ligation for 4 weeks and the plasma was extracted for serum metabolomics analysis. Results: PCA score plot data revealed significant metabolic differences between the severe heart failure group and the control group, but no significant differences were observed between the slight heart failure group and the control group. A stable and reliable OPLS-DA model was created and used to filter out 44 types of metabolite differences. The levels of free fatty acids, glucose, and lactate were significantly elevated in rats with severe heart failure. Conclusions: The serum metabolic profiles of rats with severe heart failure were markedly different from those of sham rats. A total of 44 metabolites exhibited differences between the severe heart failure and control groups.


Du Z.,Southern Medical University | Du Z.,Key Laboratory for Organ Failure Research | Shen A.,Southern Medical University | Huang Y.,Southern Medical University | And 15 more authors.
PLoS ONE | Year: 2014

Objective: Elevated myocardial energy expenditure (MEE) is related with reduced left ventricular ejection fraction, and has also been documented as an independent predictor of cardiovascular mortality. However, the serum small-molecule metabolite profiles and pathophysiological mechanisms of elevated MEE in heart failure (HF) are still lacking. Herein, we used 1H-NMR-based metabolomics analysis to screen for potential biomarkers of MEE in HF. Methods: A total of 61 subjects were enrolled, including 46 patients with heart failure and 15 age-matched controls. Venous serum samples were collected from subjects after an 8-hour fast. An INOVA 600 MHz nuclear magnetic resonance spectrometer with Carr-Purcell-Melboom-Gill (CPMG) pulse sequence was employed for the metabolomics analysis and MEE was calculated using colored Doppler echocardiography. Metabolomics data were processed using orthogonal signal correction and regression analysis was performed using the partial least squares method. Results: The mean MEE levels of HF patients and controls were 139.61±58.18 cal/min and 61.09±23.54 cal/min, respectively. Serum metabolomics varied with MEE changed, and 3-hydroxybutyrate, acetone and succinate were significantly elevated with the increasing MEE. Importantly, these three metabolites were independent of administration of angiotensin converting enzyme inhibitor, β-receptor blockers, diuretics and statins (P>0.05). Conclusions: These results suggested that in patients with heart failure, MEE elevation was associated with significant changes in serum metabolomics profiles, especially the concentration of 3-hydroxybutyrate, acetone and succinate. These compounds could be used as potential serum biomarkers to study myocardial energy mechanism in HF patients. © 2014 Du et al.


PubMed | Guangzhou General Hospital of PLA and Southern Medical University
Type: Journal Article | Journal: PloS one | Year: 2014

Elevated myocardial energy expenditure (MEE) is related with reduced left ventricular ejection fraction, and has also been documented as an independent predictor of cardiovascular mortality. However, the serum small-molecule metabolite profiles and pathophysiological mechanisms of elevated MEE in heart failure (HF) are still lacking. Herein, we used 1H-NMR-based metabolomics analysis to screen for potential biomarkers of MEE in HF.A total of 61 subjects were enrolled, including 46 patients with heart failure and 15 age-matched controls. Venous serum samples were collected from subjects after an 8-hour fast. An INOVA 600 MHz nuclear magnetic resonance spectrometer with Carr-Purcell-Melboom-Gill (CPMG) pulse sequence was employed for the metabolomics analysis and MEE was calculated using colored Doppler echocardiography. Metabolomics data were processed using orthogonal signal correction and regression analysis was performed using the partial least squares method.The mean MEE levels of HF patients and controls were 139.6158.18 cal/min and 61.0923.54 cal/min, respectively. Serum metabolomics varied with MEE changed, and 3-hydroxybutyrate, acetone and succinate were significantly elevated with the increasing MEE. Importantly, these three metabolites were independent of administration of angiotensin converting enzyme inhibitor, -receptor blockers, diuretics and statins (P>0.05).These results suggested that in patients with heart failure, MEE elevation was associated with significant changes in serum metabolomics profiles, especially the concentration of 3-hydroxybutyrate, acetone and succinate. These compounds could be used as potential serum biomarkers to study myocardial energy mechanism in HF patients.


Wang F.,Guangzhou General Hospital of PLA | Wang F.,Dongguan Peoples Hospital | Zhang L.,Guangzhou General Hospital of PLA | Cao H.-X.,Guangzhou General Hospital of PLA | And 3 more authors.
Chinese Journal of Radiology (China) | Year: 2012

Objective: To analyze the MR imaging features of epileptogenic focal cortical dysplasia (FCD)and to optimize the scanning protocols by correlating MRI appearance with pathological findings. Methods: MRI findings and the relative scanning protocols in 36 patients with surgically and pathologically proved 40 lesions of FCD were retrospectively analyzed. According to Palmini classification system, all 40 lesions were pathologically categorized as FCD type I (including FCD I A and FCD I B) and FCD type II (including FCD II A and FCD II B). The distribution of cerebral or dual lesions accompanied hippocampal sclerosis were observed. Differences of the distribution of cerebral in FCD type I and FCD type II were compared by using Fisher exact probabilities. MR scans in all patients consisted of routine and optimized protocols. Axial FSE T2WI, axial SE T1WI and axial FLAIR were recognized as routine scanning protocols, while adding oblique coronal FSE T2WI and FLAIR were recognized as optimization scanning protocols. Both routine and optimization scanning protocols were performed in all patients. The conspicuity of main findings of FCD on different imaging plane and sequences of each protocol were assessed. The detection of cerebral or dual lesion and the accompanied hippocampal sclerosis were compared between the routine protocol and the optimized protocol by using McNemar test. Results: Forty lesions were found in 36 cases with FCD, 29 had temporal lobe lesion (72.5%), 9 had frontal lobe lesion (22.5%) and 2 had parietal lobe lesion (5.0%). According to Palmini classification system, 29 lesions in 27 patients (72.5%) were FCD type I, 11 lesions in 10 patients (27.5%) were FCD type II lesions. There were 25 temporal lobe lesions in FCD type I, while 4 in FCD type II. There were statistically significant differences between FCD type I group and FCD type II group in the distribution of cerebral (P = 0.002). Fourteen cases were found to have hippocampal sclerosis simultaneously, with 13 cases found in FCD type I patients and 1 case in type II patients. The detection rate of temporal lobe lesion was 65.5% (19/29) and 44.8% (13/29) respectively on optimized protocol and routine protocol. There was statistically significant difference (χ2 = 4.167, P = 0.031). The detection rate of hippocampal sclerosis was 85.7% (12/14) and 42.9% (6/14) respectively on optimized protocol and routine protocol respectively. There was statistically significant difference (χ2 = 4.167, P = 0.031). The detection rate of frontal lobe lesion showed no statistically significant difference between optimized protocols and routine protocols (χ2 = 0.304, P = 1.000). Conclusions: FCD was frequently involved the temporal lobe, followed by the frontal lobe. FCD type I lesion was frequently found in the temporal lobe, with a higher incidence of concomitant hippocampal sclerosis. The optimized whole temporal lobe scanning with imaging plane perpendicular to the hippocampus long axis was a highly desired scanning protocol specifically for FCD, which is helpful for the detection of the FCD lesions.


Lin J.-R.,Guangzhou General Hospital of PLA | Zhang W.-M.,Guangzhou General Hospital of PLA | Wang Z.-C.,Guangzhou General Hospital of PLA
Chinese Journal of Cancer | Year: 2010

Dedifferentiated chondrosarcoma (DDCS) is a rare but highly malignant primary bone neoplasm, which is resistant to radiotherapy and chemotherapy. There remains uncertainly as to the best treatment of this disease and how to improve its prognosis. In this paper we reported a case of DDCS and reviewed the related literatures in order to provide references to throw a light on the histogenesis, diagnosis and therapy of this disease.


PubMed | Guangzhou General Hospital of PLA
Type: Journal Article | Journal: Zhongguo fei ai za zhi = Chinese journal of lung cancer | Year: 2010

Chemotherapy is a main method for patients with advanced non-small cell lung cancer (NSCLC). NSCLC is usually a drug-resistant neoplasm. Innate or acquired drug-resis-tance contributes to the chief cause for bad effect in the treatment of patients with NSCLC. To search for a new anti-cancer drug becomes a goal of clinical oncologists. The aim of the present study is to evaluate the curative effect and side reactions of IRESSA in the treatment of patients with advanced refractory NSCLC.The curative investigation was carried out after 100-day oral IRESSA by a dosage of 250mg/d in patients with advanced refractory NSCLC. The patients had ever experienced at least one regimen of chemotherapy.Totally 33 patients enrolled in this study and all were stage IV. There were 25 males and 8 females. All enrolled patients except one patient who died of severe adverse side reaction completed treatment by IRESSA. Thirty-two cases were evaluated. Complete response was obtained in 1 patient (3.1%). Partial response was seen in 11 patients (34.4%). The overall effective rate was 37.5% (12/32). The disease-control rate was 65.6% (21/32). Time to progression was 5.7 months. Overall survival time was 3.3 to 25.9 months (median survival time was 9.6 months). One-year survival rate was 28.1% (9/32). Two-year survival rate was 6.3% (2/32). The longest survivor lived for 25.9 months. The curative effect was correlated with the pathological type, in sequence of alveolar cell carcinoma, adenocarcinoma and squamous cell carcinoma. Almost all the adverse reactions were acceptable. The main adverse reactions included rash, itching of skin, arthralgia, diarrhea, anorexia, nausea, vomiting, dizziness, headache, chest distress and abdominal pain. No patients showed abnormal in liver or kidney function. No electrocardiogram abnormality was found. One patient who had chronic pulmonary fibrosis before died of respiratory failure due to severe interstitial pneumonia.IRESSA takes better effect on the advanced drug-resistant patients with NSCLC. So IRESSA may be accepted as third line in the treatment of advanced NSCLC and as first line in the treatment of patients with bad constitution who have no opportinities for operation, irradiation therapy or chemotherapy.

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