Guangzhou Eighth Peoples Hospital
Guangzhou Eighth Peoples Hospital
Jiao Y.-M.,Capital Medical University |
Weng W.-J.,Capital Medical University |
Gao Q.-S.,Academy of Military Medical science |
Zhu W.-J.,Chinese Academy of Sciences |
And 5 more authors.
Antiviral Research | Year: 2015
Abstract This study investigated whether treatment with IFN-α and ribavirin (RBV) reduces 2LTR circular HIV DNA in addition to the total and integrated HIV DNA. Two groups of patients were enrolled. Group 1 comprised HIV/HCV co-infected patients who were treated with highly active antiretroviral therapy (HAART), IFN-α and RBV for 48 weeks. After the 48 weeks of treatment, IFN-α and RBV treatment was discontinued and HAART was continued. Group 2 comprised HIV-infected patients who were treated with HAART. Real-time polymerase chain reaction (RT-PCR) was used to quantify the levels of HIV-1 DNA. We found that compared with Group 2 patients, Group 1 patients exhibited an obvious decrease in the CD4 cell count and the total DNA, 2LTR circular DNA, and integrated HIV DNA after 48 weeks of treatment. After the discontinuation of IFN-α and RBV treatment in Group 1 patients, the levels of HIV DNA recovered. Therefore, we concluded that treatment with IFN-α and ribavirin (RBV) reduces 2LTR circular HIV DNA. © 2015 Elsevier B.V. All rights reserved.
Long X.-R.,Sun Yat Sen University |
Zhang Y.-J.,Sun Yat Sen University |
Zhang M.-Y.,Sun Yat Sen University |
Chen K.,Guangzhou Eighth Peoples Hospital |
And 3 more authors.
Aging | Year: 2017
Hepatocellular carcinoma (HCC) is a common cancer with very poor survival due to lack of reliable biomarker for early diagnosis. In this study, we investigated microRNA (miRNA) profile of whole blood with a custom microarray containing probes for 1849 miRNA species in a total 213 successive subjects who were divided into a discovery set and a validation set. An 88-miRNA signature was established to diagnose health controls (HC), chronic hepatitis B (CHB), liver cirrhosis (LC) and HCC with 100% accuracy in the discovery set using Fisher discriminant analysis. This diagnostic signature was confirmed in the validation set with accuracy rates of 100%, 95.2%, 93.7% and 98.4% for HC, CHB, LC and HCC patients, respectively. Compared with AFP, the only available non-invasive and routinely used biomarker for diagnosis of HCC, the 88-miRNA signature has much higher accuracy (99.5% vs 76.5%), sensitivity (100% vs 63.8%), and specificity (99.2% vs 84.2%). More importantly, the signature detects small HCCs (< 3cm) with 100% (17/17) accuracy while AFP has only 64.7% (11/17). In conclusion, we have identified a powerful and sensitive blood 88-miRNA signature for diagnosing early HCC and other chronic liver diseases (CHB and LC) with a high accuracy. © Long et al.
Chu C.E.,University of California at San Francisco |
Wu F.,Sun Yat Sen University |
He X.,Guangzhou Eighth Peoples Hospital |
Ma Q.,UNC Project China |
And 5 more authors.
AIDS Research and Human Retroviruses | Year: 2015
Our objective was to explore the social meaning of HIV and perceptions of an HIV cure among people who inject drugs (PWID) in Guangzhou, China, which speaks to ethical and resource challenges to development in this field. We conducted a qualitative research study using in-depth interviews. We analyzed interview transcripts from 29 PWID, eight physicians, and three social workers from an outpatient HIV clinic and two methadone maintenance treatment centers. The social meaning of HIV infection and perceptions of an HIV cure reflected patients' relationships with society, health systems, and physicians. First, HIV infection decreased perceived social worth and disrupted peer relationships. The possibility of being cured renewed patient hope for regaining physical well-being and achieving social mobility. However, the existence of a cure may not alter the HIV-related stigma due to its association with stigmatized behaviors and marginalized groups. Second, although stigma was a significant barrier to engagement in health care, hope for a cure may outweigh fears of stigma and enhance linkage to HIV testing and treatment as well as methadone services. A cure may exacerbate perceived health disparities if inaccessible to key affected populations such as PWID. The social implications of an HIV cure among this key affected population may inform the design and implementation of cure clinical trials. Careful management of patient expectations, focusing research on key affected populations, expanding HIV testing and treatment systems, improving access to harm reduction programs, and ensuring post-trial access are important considerations for HIV cure research. © Mary Ann Liebert, Inc. 2015.
PubMed | Guangzhou Eighth Peoples Hospital, UNC Project China, Sun Yat Sen University and University of North Carolina at Chapel Hill
Type: Journal Article | Journal: Asian Pacific journal of tropical disease | Year: 2016
There are over 100 clinical trials worldwide focused on developing an HIV cure. Research participants will assume substantial individual risks while receiving little or no individual benefit. Physicians will have important dual roles of leading HIV cure research studies and guiding patient expectations. Many low and middle-income nations have started HIV cure trials, including China. The goal of this study was to better understand physician attitudes, behaviors, and perceptions of HIV cure research within the context of China. We conducted a quantitative and qualitative evidence review of published literature on physician perceptions of HIV cure in China. Quantitative survey data revealed that physicians rarely believed HIV was curable, but this perception may be more common compared to other countries. Qualitative data showed that inconsistent terminology used among physicians may contribute to the perception of HIV as curable. The belief that HIV is curable among some physicians in China may be related to the influence of traditional Chinese medicine beliefs. Rather than seeking elimination of pathogens, traditional Chinese medicine aims to achieve harmony between organs and a vital life force. In this context, HIV infection can be seen as a temporary state of imbalance rather than an irreversible change. There is a wide range of physician perceptions about HIV cure in China. Conflicting information about HIV cure from physicians and other sources could thwart the progress of HIV cure research. Enhancing patient-physician communication about ongoing HIV cure research trials will be important for developing an HIV cure.
Zhang J.,Guangzhou Eighth Peoples Hospital |
Chen W.,Sun Yat Sen University
International Journal of Clinical and Experimental Medicine | Year: 2017
Hepatectomy is the main curative strategy for patients with colorectal liver metastasis (CRLM). In recent years, laparoscopic hepatectomy (LH) has gradually been adopted for the treatment of CRLM. However, in most cases reported in previous studies, CRLM was located in the anterolateral segments. The aim of the current analysis was to compare the short- and long-term outcomes of LH for CRLM in the posterosuperior segments. Clinical and follow-up data of patients with CRLM, undergoing LH at our hospital from March 2009 to October 2016 were retrospectively analyzed. Patients were divided into the posterosuperior group (38 cases) and the anterolateral group (81 cases) based on the location of CRLM. Compared with the anterolateral group, the posterosuperior group had longer operative time, greater intraoperative blood loss, and higher rate of conversion. There was no statistical difference in the rate and severity of postoperative 30-day complications, postoperative 30-day mortality, length of hospital stay, pathological results, 5-year overall survival, and disease-free survival. In summary, although LH for CRLM in the posterosuperior segments has shortcomings such as long operative time, high intraoperative blood loss, and high rate of conversion, the incidence of postoperative complications, severity of complications, postoperative 30-day mortality, and long-term survival outcomes in the PS group were not different from those in the anterolateral segments. © 2017, E-Century Publishing Corporation. All rights reserved.
Deng H.H.,Guangzhou Eighth Peoples Hospital
Zhonghua nei ke za zhi | Year: 2016
To analyze the clinical features and prognosis of fulminant Wilson's disease (FWD) in patients with hepatitis B virus (HBV) infection. Twenty-seven patients were enrolled in Guangzhou Eighth People's Hospital from 2005 to 2015, including 13 FWD patients with HBV infection and 14 FWD patients without HBV infection. Clinical efficacy and survival rate were evaluated. Baseline biochemical data in two groups were comparable(P>0.05), including total bilirubin, prothrombin activity, serum albumin, alpha fetal protein, alanine transaminase, ceruloplasmin and 24 hours urine copper .Treatment in FWD group with HBV infection was ineffective, including 9(9/13) deaths and 4(4/13) patients receiveing liver transplants. However, 7(7/14)cases in the other group did not response to the treatment, including 6(6/14)deaths and 1(1/14)patient receiving liver transplant. The prognosis in the two groups is significantly different(P=0.006), which is much worse in FWD patients with HBV infection.
Deng J.-J.,Guangzhou Eighth Peoples Hospital
World Chinese Journal of Digestology | Year: 2010
AIM: To explore the mechanism by which acupuncture promotes intestinal motility. METHODS: Thirty Sprague-Dawley rats were randomly divided into blank group, model group (receiving colocolic anastomosis) and acupuncture group. The acupuncture group underwent acupuncture at Zusanli, Sanyinjiao and Taichong daily for three continuous days. After acupuncture treatment, defecation was observed and intestinal propulsive rate was measured. Tissue samples of the colon which was 2 cm below the caecum were taken to observe the ultrastructure of interstitial cells of Cajal (ICC) and the Ache-ICC-SMC network. RESULTS: In the acupuncture group, the time to first postoperative passage of feces was shortened and intestinal propulsive rate was improved compared with the model group [(2.00 ± 0.47) d vs (2.50 ± 0.53) d, (66.30 ± 4.21)% vs (46.33 ± 5.56)% , both P < 0.05]. Compared with the blank group, the damage of ICC ultrastructure in the model group was more significant while that in the acupuncture group was milder. In the model group, the ENS-ICC-SMC structure was disorganized, and the number of ICC and their fluorescence intensity were greatly decreased compared with the blank group [(18.67 ± 6.11) vs (32.33 ± 5.51), (35.00 ± 9.54) vs (58.67 ± 10.21), both P < 0.05]. In contrast, in the acupuncture group, the damage of the network structure was milder, and the number of ICC and their fluorescence intensity were increased compared with the model group [(30.33 ± 3.21) vs (18.67 ± 6.11), (56.67 ± 9.45) vs (35.00 ± 9.54), both P < 0.05]. Similar results were also obtained for the number of VAChT-positive nerve fibres [(18.67 ± 3.79) vs (20.67 ± 3.21), (20.33 ± 5.13) vs (34.67 ± 6.81), (23.00 ± 4.58) vs (18.67 ± 3.79), (36.00 ± 8.19) vs (20.33 ± 5.13), all P < 0.05]. CONCLUSION: Acupuncture can improve intestinal motility in rats after abdominal operation perhaps by improving the repair and regeneration of ICC.
PubMed | Guangzhou Eighth Peoples Hospital, Johns Hopkins University, University of Utah, World Health Organization and North University of China
Type: Review | Journal: Journal of the International AIDS Society | Year: 2016
Qualitative research on antiretroviral therapy (ART) adherence interventions can provide a deeper understanding of intervention facilitators and barriers. This systematic review aims to synthesize qualitative evidence of interventions for improving ART adherence and to inform patient-centred policymaking.We searched 19 databases to identify studies presenting primary qualitative data on the experiences, attitudes and acceptability of interventions to improve ART adherence among PLHIV and treatment providers. We used thematic synthesis to synthesize qualitative evidence and the CERQual (Confidence in the Evidence from Reviews of Qualitative Research) approach to assess the confidence of review findings.Of 2982 references identified, a total of 31 studies from 17 countries were included. Twelve studies were conducted in high-income countries, 13 in middle-income countries and six in low-income countries. Study populations focused on adults living with HIV (21 studies, Our study evaluated adherence interventions based on qualitative data from PLHIV and health providers. The study underlines the importance of incorporating social and cultural factors into the design and implementation of interventions. Further qualitative research is needed to evaluate ART adherence interventions.
PubMed | Guangzhou Eighth Peoples Hospital, Guangzhou University, University of California at San Francisco, Sun Yat Sen University and University of North Carolina at Chapel Hill
Type: Journal Article | Journal: Open forum infectious diseases | Year: 2016
Background. Hepatitis C virus (HCV) treatment access among human immunodeficiency virus (HIV)/HCV-coinfected people who inject drugs is poor, despite a high burden of disease in this population. Understanding barriers and facilitators to HCV treatment uptake is critical to the implementation of new direct-acting antivirals. Methods. We conducted in-depth interviews with patients, physicians, and social workers at an HIV treatment facility and methadone maintenance treatment centers in Guangzhou, China to identify barriers and facilitators to HCV treatment. We included patients who were in various stages of HCV treatment and those who were not treated. We used standard qualitative methods and organized data into themes. Results. Interview data from 29 patients, 8 physicians, and 3 social workers were analyzed. Facilitators and barriers were organized according to a modified Consolidated Framework for Implementation Research schematic. Facilitators included patient trust in physicians, hope for a cure, peer networks, and social support. Barriers included ongoing drug use, low HCV disease knowledge, fragmented reimbursement systems, HIV exceptionalism, and stigma. Conclusions. Expanding existing harm reduction programs, HIV treatment programs, and social services may facilitate scale-up of direct-acting antivirals globally. Improving integration of ancillary social and mental health services within existing HIV care systems may facilitate HCV treatment access.
PubMed | Yunnan Care Center, Capital Medical University, Guangzhou Eighth Peoples Hospital, The 6th People Hospital of Zhengzhou and PLA Fourth Military Medical University
Type: | Journal: AIDS research and therapy | Year: 2015
Human leukocyte antigen (HLA)-B*5701 is strongly associated with developing a hypersensitivity reaction to abacavir (ABC). Limited data exist on HLA-B*5701 prevalence in HIV-1-infected subjects in China. We investigated HLA-B*5701 prevalence in HIV-1-infected population including Han and non-Han ethnic groups.A prospective multi-centre study was designed to determine status of HLA-B*5701 in HIV-1-infected adults at six sites across China. HLA-B*5701 was tested by the method of PCR-SSP.From six centers, 3,000 HIV-infected patients [2,452 (81.7%) Han, 548 (18.3%) Non-Han] were recruited with a mean age of 36.7years old. The overall HLA-B*5701 prevalence was 0.86% [95% confidence interval (CI) 0.55-1.26%]. The prevalence of HLA-B*5701 among Han subjects was similar to that among non-Han subjects, which was 0.88% (95% CI 0.54-1.34%) and 0.76% (95% CI 0.19-1.93%), respectively (p value=0.787). There were no differences in prevalence of HLA-B*5701 between subjects born in Henan, Yunnan, Shanxi, Guangdong, Hebei, Beijing and other provinces (p=0.999).HLA-B*5701 prevalence is very low in HIV-infected Chinese subjects, both in the Han and Non-Han nationalities. And there are no differences among different birthplaces across China.