Guangzhou Blood Center

Guangzhou, China

Guangzhou Blood Center

Guangzhou, China
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Lu L.,Sun Yat Sen University | Lu L.,University of Kansas Medical Center | Wang M.,Guangzhou Blood Center | Xia W.,Guangzhou Blood Center | And 13 more authors.
Journal of Virology | Year: 2014

We investigated the migration patterns of hepatitis C virus (HCV) in China. Partial E1 and/or NS5B sequences from 411 volunteer blood donors sampled in 17 provinces and municipalities located in five large regions, the north-northeast, northwest, southwest, central south, and southeast, were characterized. The sequences were classified into eight subtypes (1a, n = 3; 1b, n = 183; 2a, n = 83; 3a, n = 30; 3b, n = 44; 6a, n = 55; 6n, n = 10; 6v, n = 1) and a new subtype candidate. Bayesian evolutionary analysis by sampling trees of the E1 sequences of the five major subtypes revealed distinct migration patterns. Subtype 1b showed four groups: one is prevalent nationwide with possible origins in the north-northeast; two are locally epidemic in the central south and northwest, respectively, and have spread sporadically to other regions; and the fourth one is likely linked to the long-distance dispersion among intravenous drug users from the northwest. Subtype 2a showed two groups: the larger one was mainly restricted to the northwest and seemed to show a trend toward migration via the Silk Road; the smaller one was geographically mixed and may represent descendants of those that spread widely during the contaminated plasma campaign in the 1990s. Subtype 3a exhibited three well-separated geographic groups that may be epidemically unrelated: one showed origins in the northwest, one showed origins in the southwest, and the other showed origins in the central south. In contrast, subtype 3b had a mixture of geographic origins, suggesting migrations from the southwest to the northwest and sporadically to other regions. Structurally resembling the tree for subtype 3a, the tree for subtype 6a showed four groups that may indicate migrations from the central south to southeast, southwest, and northwest. Strikingly, no subtype 6a strain was identified in the north-northeast. © 2014, American Society for Microbiology.


Li C.,University of Kansas Medical Center | Yuan M.,State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics | Lu L.,University of Kansas Medical Center | Lu T.,University of Kansas Medical Center | And 6 more authors.
Virology | Year: 2014

Vietnam has a unique history in association with foreign countries, which may have resulted in multiple introductions of the alien HCV strains to mix with those indigenous ones. In this study, we characterized the HCV sequences in Core-E1 and NS5B regions from 236 Vietnamese individuals. We identified multiple HCV lineages; 6a, 6e, 6h, 6k, 6l, 6o, 6p, and two novel variants may represent the indigenous strains; 1a was probably introduced from the US; 1b and 2a possibly originated in East Asia; while 2i, 2j, and 2m were likely brought by French explorers. We inferred the evolutionary history for four major subtypes: 1a, 1b, 6a, and 6e. The obtained Bayesian Skyline Plots (BSPs) consistently showed the rapid HCV population growth from 1955 to 1963 until 1984 or after, corresponding to the era of the Vietnam War. We also estimated HCV growth rates and reconstructed phylogeographic trees for comparing subtypes 1a, 1b, and HCV-2. © 2014 Elsevier Inc.


Fu Y.,Guangzhou Blood Center | Wang Y.,Sun Yat Sen University | Xia W.,Guangzhou Blood Center | Pybus O.G.,University of Oxford | And 4 more authors.
Journal of Viral Hepatitis | Year: 2011

Recently, we studied hepatitis C virus (HCV) sera-prevalence among 559 890 first-time volunteer blood donors in China. From randomly selected 450 anti-HCV positive donors, we detected HCV RNA in 270 donors. In this study, we amplified HCV E1 and/or NS5B sequences from 236 of these donors followed by DNA sequencing and phylogenetic analysis. The results indicate new trends of HCV infection in China. The HCV genotype distribution differed according to the donors' region of origin. Among donors from Guangdong province, we detected subtypes 6a, 1b, 3a, 3b, 2a, and 1a at frequencies of 49.7%, 31.0%, 7.6%, 5.5%, 4.1%, and 2.1%, respectively. Among donors from outside Guangdong, we detected 1b, 2a, 6a, 3b, 3a, 6e, and 6n at frequencies 57.1%, 13.2%, 11.0%, 9.9%, 4.4%, 2.2%, and 2.2%, respectively. Although we found no significant differences among regions in age or gender, subtype 6a was more common (P < 0.001) in donors from Guangdong than those from elsewhere, whilst subtypes 1b (P < 0.02) and 2a (P < 0.001) were more frequent outside Guangdong. Disregarding origins, the male/female ratio was higher for subtype 6a-infected donors (P < 0.05) than for subtype 1b donors, whilst the mean age of subtype 2a donors was 8-10 years older (P < 0.05) than that for all other subtypes. Detailed phylogenetic analysis of our sequence data provides further insight into the transmission of HCV within China, and between China and other countries. The predominance of HCV 6a among blood donors in Guangdong is striking and mandates studies into risk factors for its acquisition. © 2010 Blackwell Publishing Ltd.


Xu R.,Guangzhou Blood Center | Tong W.,Sun Yat Sen University | Gu L.,Sun Yat Sen University | Li C.,University of Kansas Medical Center | And 2 more authors.
Infection, Genetics and Evolution | Year: 2013

In this study, we characterized the full-length genomes of 16 HCV isolates obtained from patients in a single hospital in China using overlapping PCR followed by DNA sequencing. The obtained genomes are 9414-9628 nucleotides in length, and each genome contains a single ORF of 9021-9102 nucleotides. Nine genomes represent the common subtypes 1a, 1b, 2a, 2b, 3a, 3b, and 6a, while seven represent the infrequent lineages 1c, 2f, 4d, and 5a, and two novel genotype 6 variants. GZ51969 and GZ52540 are subtype 1b isolates belonging to two unique clusters designated A and B, which account for 29.5% and 59.5% of the 1b infections in China, respectively. ZS542 and GZ98799 represent the first two complete genomes of the provisionally assigned subtype 2f. ZS96 and ZS202 are novel genotype 6 variants that may qualify for two new subtypes. ZS17, ZS537, and ZS631 represent three alien subtypes, namely, 1c, 4d, and 5a, which were detected in China for the first time in this study and may have been recently introduced as a result of globalization. Taken together, these results confirmed a large variety of HCV taxonomic lineages in China through the sequencing of their full-length genomes. These lineages represent six genotypes, 11 subtypes, and two novel variants. They were characterized for achieving a better understanding of the HCV genetic variation patterns and for possible future research applications. © 2013 Elsevier B.V.


An Y.,Sun Yat Sen University | Wu T.,Southern Medical University | Wu T.,Hainan General Hospital | Wang M.,Guangzhou Blood Center | And 6 more authors.
Virology | Year: 2014

We characterized a novel group of HCV variants that are genetically related but distinct from each other belonging to genotype 6 (HCV-6). From 26 infected Austronesian-descended aborigines on Hainan Island, China, HCV sequences were determined followed by genetic analyses. Six nearly full-length genomes and 20 E1 sequences of HCV were obtained, which differ from each other and from all known HCV lineages by nucleotides above the intra-subtype level of 13%. Together with subtypes 6g and 6w, they constitute a phylogenetic group sharing a common ancestor dating from the end of the 12th century. Conclusion: Our data indicate the maintenance of an isolated HCV-6 indigenous circulation on Hainan Island at least for six centuries. © 2014 Elsevier Inc.


Hu L.,CAS Guangzhou Institute of Biomedicine and Health | Yao W.,CAS Guangzhou Institute of Biomedicine and Health | Wang F.,CAS Guangzhou Institute of Biomedicine and Health | Rong X.,Guangzhou Blood Center | And 2 more authors.
PLoS ONE | Year: 2014

Hepatitis C virus (HCV) is a major cause of chronic liver disease. However, little is known about the details of its assembly and secretion. Golgi-related proteins have been recently proven to have a key function in HCV secretion. Golgi protein 73 (GP73), a resident Golgi membrane protein, is a potential serum biomarker for the diagnosis of liver diseases and hepatocellular carcinoma. Previous studies have demonstrated the upregulation of GP73 in the liver samples and sera of HCV-infected patients. However, the function and regulatory mechanism of GP73 in HCV infection at the cellular level remain unknown. In this study, we examined the expression level of GP73 in HCV infected cells and its effect on HCV life cycle in cell culture systems. Both the protein expression and mRNA levels of GP73 significantly increased in HCV subgenomic replicon-harboring cells and HCV-infected cells, which imply that GP73 was upregulated by HCV infection. HCV production was significantly enhanced when GP73 was overexpressed, but dramatically inhibited when GP73 was silenced. However, the overexpression and knockdown of GP73 showed no evident effect on the entry, protein translation, RNA replication, and assembly of HCV, which indicates that GP73 enhanced the secretion process. Moreover, the coiled-coil domain of GP73 was required to increase HCV secretion. GP73 increased and interacted with apolipoprotein E, an identified host factor that assists in HCV secretion. These results demonstrate the critical function of GP73 in HCV secretion and provide new insights into the therapeutic design of antiviral strategies. © 2014 Hu et al.


PubMed | University of Massachusetts Boston, Yale University, Shenzhen Blood Center, Southern Medical University and Guangzhou Blood Center
Type: | Journal: Transfusion medicine reviews | Year: 2016

The need for physicians and technical consultants specialized in transfusion medicine is urgent in China, as there are 20 000 hospitals and 500 blood centers in need of staff with this expertise. The progress made in transfusion medicine as a specialty has been relatively slow in China. Current Chinese medical education and service systems have not developed transfusion medicine as a stand-alone medical specialty. Most physicians receive only minimal training in transfusion medicine in medical school. This training is usually integrated into surgical training and addresses the most common technologies. In 2008, a 5-year bachelors diploma program in transfusion medicine was established as an undergraduate specialty in Southern Medical University, Guangzhou, China. This article intends to summarize the 8 years of experience educating undergraduates in the specialty of transfusion medicine.


Zhang R.,CAS Guangzhou Institute of Biomedicine and Health | Zhang R.,Hefei University of Technology | Rong X.,Guangzhou Blood Center | Pan W.,CAS Guangzhou Institute of Biomedicine and Health | And 2 more authors.
Scandinavian Journal of Infectious Diseases | Year: 2011

Background: Humoral virus neutralizing activity is crucial in preventing influenza virus infection. However, the influenza neutralizing activity in the general population remains unclear. Methods: In this study we performed a serological survey of 200 blood donors from Guangzhou, China. Using a microneutralization (MN) assay, neutralizing activities against influenza A 2009 H1N1, H3N2 and H5N1 were measured. Anti-haemagglutinin antibody was assayed by haemagglutination inhibition (HI) test. Also, antibodies against M1 and M2 matrix proteins were measured using an enzyme-linked immunosorbent assay (ELISA). Results: By MN assay, 86% of the individuals showed neutralizing activity against H3N2, 11% against 2009 H1N1, and none against H5N1. The positive rate for H3N2 increased as the age of individuals increased. Interestingly, males displayed a 4 times higher positive rate against 2009 H1N1 than females. The results of ELISA revealed that 97.5% of the individuals had positive M1 titres and 21% had positive M2 titres. Furthermore, anti-haemagglutinin antibody had a much higher correlation with the neutralization activity than anti-M1 and anti-M2 antibodies. Conclusions: Neutralizing activities against H5N1 and 2009 H1N1 were low in the general population. Therefore, public health agencies should design strategies for preventing potential H5N1 and 2009 H1N1 pandemics. © 2011 Informa Healthcare.


Ou-Yang J.,Guangzhou Blood Center | Bei C.-H.,Guangzhou Blood Center
Transfusion Medicine | Year: 2016

Objective: To explore the trends in blood collection from 2006 to 2014 in Guangdong, China. Background: Although the Blood Donation Law of the People's Republic of China was implemented in 1998, voluntary non-remunerated blood donation (VNRBD) has been promoted fully for only a decade. The provincial and local governments of Guangdong, one of the most well-developed provinces in China, have promoted blood donation by various means. Material and methods: Official data on blood donation from 2006 to 2014, including the number of blood donations and the family replacement/mutual blood donation (FRMBD) rate, were collected from all blood collection and supply institutions in Guangdong. These data were analysed to explore trends in blood donation in Guangdong Province, and to detect differences among the province's four regions. Results: The number of blood donations in Guangdong increased by 38·23% from 2006 to 2014; overall, the rate increased annually, although it fluctuated in the eastern region. Family replacement/mutual whole blood and platelet donation rates decreased dramatically from 2006 to 2014 (from 39·99% to 20·16% and from 64·15% to 26·51%, respectively), but remained high. Marked disparities in blood donation development were detected among the four regions. Discussions: With nearly a decade of efforts, blood donation in Guangdong has developed rapidly and sustainably. All blood collection and supply institutions must strengthen efforts to improve awareness of blood donation among the population, retain repeat and regular donors and reduce the rate of FRMBD in favor of the development of VNRBD. © 2016 British Blood Transfusion Society


PubMed | Guangzhou Blood Center
Type: Journal Article | Journal: Transfusion medicine (Oxford, England) | Year: 2016

To explore the trends in blood collection from 2006 to 2014 in Guangdong, China.Although the Blood Donation Law of the Peoples Republic of China was implemented in 1998, voluntary non-remunerated blood donation (VNRBD) has been promoted fully for only a decade. The provincial and local governments of Guangdong, one of the most well-developed provinces in China, have promoted blood donation by various means.Official data on blood donation from 2006 to 2014, including the number of blood donations and the family replacement/mutual blood donation (FRMBD) rate, were collected from all blood collection and supply institutions in Guangdong. These data were analysed to explore trends in blood donation in Guangdong Province, and to detect differences among the provinces four regions.The number of blood donations in Guangdong increased by 3823% from 2006 to 2014; overall, the rate increased annually, although it fluctuated in the eastern region. Family replacement/mutual whole blood and platelet donation rates decreased dramatically from 2006 to 2014 (from 3999% to 2016% and from 6415% to 2651%, respectively), but remained high. Marked disparities in blood donation development were detected among the four regions.With nearly a decade of efforts, blood donation in Guangdong has developed rapidly and sustainably. All blood collection and supply institutions must strengthen efforts to improve awareness of blood donation among the population, retain repeat and regular donors and reduce the rate of FRMBD in favor of the development of VNRBD.

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