Zhao C.-X.,Guangyuan Hospital of Traditional Chinese Medicine |
Ma J.,Guangyuan Hospital of Traditional Chinese Medicine |
He N.,Guangyuan Hospital of Traditional Chinese Medicine |
Chen Z.-H.,Guangyuan Hospital of Traditional Chinese Medicine
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Fracture healing mechanism is complex and affected by many factors, and delayed fracture healing or nonunion often occurs. How to promote fracture healing has become a serious problem. OBJECTIVE: To observe the effect of local application of nerve growth factor on early fracture healing after peripheral nerve injury. METHODS: Thirty-six healthy male Wistar rats were selected to establish tibial fracture models, which were randomly divided into four groups, with 18 limbs in each group. Group A: tibial fracture+normal saline injection via bilateral gastrocnemius muscles; group B: tibial fracture+nerve injury+normal saline injection; group C: tibial fracture+local injection of nerve growth factor; group D: tibial fracture+nerve injury+local injection of nerve growth factor. Callus metrology results were compared among different groups. RESULTS AND CONCLUSION: The callus volume was the most in the group B at 4 weeks of intervention, but there were no different among the other three groups (P > 0.05). At 2 weeks of intervention, the bone resorption area was significantly larger in the group B than the group D (P < 0.05), and the osteoclast index was significantly higher in the group A than the group C (P < 0.05); while at 4 weeks of intervention, the mineralized bone trabecular width was significantly lower in the group A than the group C (P < 0.05) as well as lower in the group C than the group D (P < 0.05). These findings indicate that after peripheral nerve injury, local application of nerve growth factor can enhance the osteogenic ability, effectively inhibit osteoclast activity, and promote the early healing of fracture. © 2015, Chinese Journal of Tissue Engineering Research. All rights reserved.