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Li Q.F.,Chinese Academy of Forestry | Li Q.F.,GuangXi Zhuang Autonomous Region Forestry Research Institute | Wang J.H.,Chinese Academy of Forestry | Pulkkinen P.,Finnish Forest Research Institute | Kong L.S.,University of Victoria
PLoS ONE | Year: 2015

Embryogenic cultures of Picea balfouriana, which is an important commercial species for reforestation in Southern China, easily lose their embryogenic ability during long-term culture. Embryogenic tissue that proliferated at lower concentrations (3.6 μM and 2.5 μM) of 6- benzylaminopurine (6-BAP) were more productive, and generated 113 ± 6 and 89 ± 3 mature embryos per 100 mg embryogenic tissue, respectively. A metabolomic approach was used to study the changes in metabolites linked to embryogenic competence related to three different 6-BAP concentrations (2.5 μM, 3.6 μM, and 5 μM). A total of 309 compounds were obtained, among which 123 metabolites mapped to Kyoto Encyclopedia of Genes and genomes (KEGG) pathways. The levels of 35 metabolites were significantly differentially regulated among the three 6-BAP treatments, and 32 metabolites differed between the 2.5 μM and 5 μM treatments. A total of 17 metabolites appeared only once among the three comparisons. The combination of a score plot and a loading plot showed that in the samples with higher embryogenic ability (3.6 μM and 2.5 μM), up-regulated metabolites were mostly amino acids and down-regulated metabolites were mostly primary carbohydrates (especially sugars). These results suggested that 6-BAP may influence embryogenic competence by nitrogen metabolism, which could cause an increase in amino acid levels and higher amounts of aspartate, isoleucine, and leucine in tissues with higher embryogenic ability. Furthermore, we speculated that 6-BAP may affect the amount of tryptophan in tissues, which would change the indole-3-acetic acid levels and influence the embryogenic ability. © 2015 Li et al.This is an open access article distributed under the terms of the Creative Commons Attribution License ,which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Yao R.L.,Guangxi Zhuang Autonomous Region Forestry Research Institute | Yao R.L.,Southern Cross University of Australia | Yao R.L.,Key Laboratory of Central South Fast growing Timber Cultivation of Forestry Ministry of China | Yao R.L.,Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation | And 2 more authors.
Southern Forests | Year: 2014

In eucalypt plantations in subtropical Australia, Eucalyptus dunnii exhibits greater diameter increment after thinning than more shade-tolerant Corymbia citriodora. To elucidate the mechanism underlying this difference, we investigated relationships between tree leaf area and sapwood area following thinning in 11-year-old E. dunnii and C. citriodora plantations. There was no significant difference in specific leaf area (SLA) between thinned and unthinned stands in E. dunnii and C. citriodora, whereas crown zone significantly affected SLA in the two eucalypts. At the whole-tree level non-linear leaf area (Al)-sapwood area (As) relationships were measured in both eucalypts by thinning treatment. A significant increase in Al/As ratio was only observed at the upper crown in thinned E. dunnii. The present results suggested the plastic nature of response of leaf characteristics in both eucalypts grown in different light environments and the species-specific pattern of crown-zone leaf responses to thinning in the two species, i.e. the top of the canopy appears to be driving greater growth response to thinning in the less shade tolerant E. dunnii compared with the more shade-tolerant C. citriodora. It is concluded that different thinning regimes should be applied in shade-tolerant and shade-intolerant eucalypt forests. © 2014 Copyright © NISC (Pty) Ltd.


PubMed | Product & Technology Development Center, National Chung Hsing University, Guangxi Zhuang Autonomous Region Forestry Research Institute, University of Chinese Academy of Sciences and 2 more.
Type: Journal Article | Journal: Zootaxa | Year: 2014

Two closely related new species of Aprostocetus Westwood (Hymenoptera: Eulophidae: Tetrastichinae) are described as fortuitous parasitoids of invasive gall inducers in two other genera of Tetrastichinae, Leptocybe Fisher & LaSalle and Quadrastichus Girault. Aprostocetus causalis La Salle & Wu is a parasitoid of Leptocybe invasa Fisher & La Salle on Eucalyptus spp. (Myrtaceae) in China and Thailand, and A. felix La Salle, Yang & Lin is a parasitoid of Quadrastichus erythrinae Kim on Erythrina spp. (Fabaceae) in Taiwan. Epitetrastichus nigriventris Girault, 1913 is removed from synonymy from Aprostocetus gala (Walker), and treated as the valid species A. nigriventris (Girault).


Cai J.,Tianjin University of Science and Technology | Wu Q.,Guangxi Zhuang Autonomous Region Forestry Research Institute | Wang G.,Tianjin University of Science and Technology | Wang G.,CAS Qingdao Institute of Oceanology | Deng C.,National Marine Environmental Monitoring Center
Renewable Energy | Year: 2013

The fermentative hydrogen production capability of the newly isolated Clostridium sp. 6A-5 bacterium was studied in a batch cultivation experiment. Various culture conditions (temperature, initial pH, and glucose concentration) were evaluated for their effects on cell growth and hydrogen production (including the yield and rate) of Clostridium sp. 6A-5. Optimal cell growth was observed at 40°C, initial pH 7.5-8, and glucose concentration 16-26g/L. The optimal hydrogen yield was obtained at 43°C, initial pH 8, and glucose concentration 10-16g/L. Hydrogen began to evolve when cell growth entered the mid-exponential phase and reached the maximum production rate at the late exponential and stationary phases. The maximum hydrogen yield, and rate were 2727mL/L, and 269.3mL H2/L h, respectively. These results indicate that Clostridium sp. 6A-5 is a good candidate for mesophilic fermentative hydrogen production. © 2013.


Hu Y.,Guangxi University | Chen H.,Guangxi Zhuang Autonomous Region Forestry Research Institute | Luo C.,Guangxi University | Dong L.,Guangxi University | And 4 more authors.
Scientia Horticulturae | Year: 2014

The proper selection of stable reference genes for real-time quantitative PCR (RT-qPCR) can help to reliably assess gene expression results under specific experimental conditions. The present study evaluated the stability of 16 candidate reference genes of kumquat using the methods of geNorm, Normfinder, and Bestkeeper in different tissues of kumquat (Fortunella crassifolia Swingle) under some type of abiotic stress. The results showed that homologous genes should be taken into account for normalization in RT-qPCR studies. For the three specific types of stresses, i.e., salt stress, drought stress, and heavy metal stress, ACT6, ACT8, and ACT7 should be selected as internal controls, respectively. For low-temperature stress, the use of multiple reference genes for normalization may be optimal, such as ACT7+. ACT9 (according to geNorm) or ACT7+. TUB4 (according to Normfinder). We suggest that ACT7 alone or the combination of ACT7+. ACT1+. EF1-α or ACT1+. ACT8 can meet the requirements for the normalization of RT-qPCR studies under the experimental conditions tested here. Although some commonly used reference genes (TUA1, TUB1, TUB4, CYP, GAPDH, etc.) showed much higher variability, some are still useful in combinations under specific conditions. © 2014.


Tong F.,Hunan Academy of Forestry | Jiang Y.,Guangxi Zhuang Autonomous Region Forestry Research Institute | Liu Z.,Hunan Academy of Forestry | Huang R.,Guangxi Zhuang Autonomous Region Forestry Research Institute | And 2 more authors.
Journal of Food, Agriculture and Environment | Year: 2014

To study the wood chemical composition and heat value genetic variations of the half-sib family of Castanopsis fissa and estimate its genetic parameter, randomised complete block test method was adopted for the growth measurement, as well as wood density, cellulose content, lignin content and heat value analysis of the 6-year-old family. It was found that the wood densities of the half-rib family of Castanopsis fissa are in significant differences and the cellulose content, lignin content and heat value are in highly significant differences. The average wood density of 52 halfsib families of Castanopsis fissa is 0.4472 g/cm3 and the genetic variation coefficient is 4.22% with a broad-sense heritability of 0.1916. The average wood cellulose content is 49.55% and the genetic variation coefficient is 6.41% with a broad-sense heritability of 0.2939, under weak genetic control. The average lignin content is 21.07% and the genetic variation coefficient is 5.17% with a broad-sense heritability of 0.4942, under weak and moderate genetic control. The average heat value of wood is 18680.11 J/g and the genetic variation coefficient is 7.16% with a broad-sense heritability of 0.7026, under strong genetic control. The DBH and height growth of the half-sib family of Castanopsis fissa are uncorrelated to the wood density, cellulose content or lignin content, but weakly correlated to heat value. It suggests that properties such as wood density, cellulose content and lignin content are mutually independent in terms of heredity. These properties can be selected independently to cultivate family with better wood properties.


Luo J.,Guangxi Zhuang Autonomous Region Forestry Research Institute | Luo J.,Central South University of forestry and Technology | Zhou G.-Y.,Central South University of forestry and Technology | Zhu J.-Y.,Guangxi Zhuang Autonomous Region Forestry Research Institute
Forest Research | Year: 2014

Insect viruses are the microbes infecting arthropods only, including nuclear polyhedrosis virus, granulosis virus, cytoplasmic polyhedrosis virus, poxvirus, iridovirus and many other groups. The nuclear polyhedrosis virus belongs to Rhabdoviridae Nucleopolyhedrovirus, which is the largest group of insect viruses. It can infect and kill one or several agricultural and forestry pests specifically, and is harmless to the natural enemies, environment, human and animals. So it is considered as the environment-friendly biopesticides to be worth promoting. This paper reviews recent research on the genomics of nuclear polyhedrosis virus, and summarizes the main functional genes including transcription related genes, DNA replication related genes, structure related genes and other genes. Further more, future development direction of the nuclear polyhedrosis virus is also prospected.


Luo J.,Central South University of forestry and Technology | Luo J.,Guangxi Zhuang Autonomous Region Forestry Research Institute | Zhou G.-Y.,Central South University of forestry and Technology | Zhu J.-Y.,Guangxi Zhuang Autonomous Region Forestry Research Institute
Forest Research | Year: 2015

Late expression factor 8 (lef-8) gene is an early expression gene, and codes the largest subunit of RNA polymerase in baculovirus. The authors sequenced the lef-8 gene of Buzura suppressaria nuclear polyhedrosis virus (BsNPV). It had 2 634 bp coding region and encoding 877 amino acid residues. Some signal sequences were found in untranslated region (UTR): possible promoter and transcription initiation site were found in 5'-UTR, and a putative polyadenylation signal sequence was found in 3'-UTR. But whether they had substantial function needs further research. The protein sequence of lef-8 gene in BsNPV was low homologous to model NPVs. But they had nearly the same signal sequence. By phylogenetic analysis of lef-8 gene in baculovirus, it was found that the nuclear polyhedrosis virus (NPV) and the granulosis virus (GV) was separated obviously to two branches, while the NPV broke into 4 branches. And evolutionary relationship in baculovirus was largely associated with its host. ©, 2015, Chinese Academy of Forestry. All right reserved.


Peng L.,Chinese Academy of Forestry | Song B.,Chinese Academy of Forestry | Wang J.,GuangXi Zhuang Autonomous Region Forestry Research Institute | Wang D.,Chinese Academy of Forestry
Advances in Materials Science and Engineering | Year: 2015

A sound-absorbing composite material made of wood fiber and polyester fiber was produced using polyester foam technology and wood-based composite technology. This study investigated the physical and mechanical properties and the effects of the airflow resistivity of the materials and the depth of the cavities behind on sound-absorbing performance of the composite material. The results showed that the composite of best physical and mechanical properties and sound absorption was made with a 150°C hot-pressing temperature, 10 min hot-press time, 3: 1 the ratio of wood fiber to polyester fiber, foaming agent content of 8%, and the nominal density of 0.2 g/cm3; the composite material yielded superior sound absorption property with the airflow resistivity of 1.98 × 105 Pa·s/m2; the acoustic absorption peak moved to lower frequencies when length of the cavities was increased. © 2015 Limin Peng et al.


Li D.-W.,Guangxi Zhuang Autonomous Region Forestry Research Institute | Deng Y.,Guangxi Zhuang Autonomous Region Forestry Research Institute | Jiang X.-J.,Guangxi Zhuang Autonomous Region Forestry Research Institute | Chang M.-S.,Guangxi Zhuang Autonomous Region Forestry Research Institute | Luo J.,Guangxi Zhuang Autonomous Region Forestry Research Institute
Forest Research | Year: 2016

Objective: To understand the resistance of oil tea to Scirtothrips dorsalis and analyse the mechanism. Method: The population densities of S. dorsalis on seven varieties of oil tea in field were investigated, the survival rates were tested by feeding them with the tender leaf of the seven varieties of oil tea in laboratory, and the fuzz distribution characteristics of the tender shoots were recorded respectively. Result: The results showed that there were significant differences in mean population density of S. dorsalis among these varieties of oil tea in the field; however, the average survival rate of S. dorsalis did not show significant difference in lab. Conclusion: Based on correlation analysis it was showed that the resistance of oil tea to S. dorsalis did not come from its own chemicals, but might mainly due to the distribution of fuzz on leaf surface. © 2016, Chinese Academy of Forestry. All right reserved.

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