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Peng L.,Chinese Academy of Forestry | Song B.,Chinese Academy of Forestry | Wang J.,GuangXi Zhuang Autonomous Region Forestry Research Institute | Wang D.,Chinese Academy of Forestry
Advances in Materials Science and Engineering | Year: 2015

A sound-absorbing composite material made of wood fiber and polyester fiber was produced using polyester foam technology and wood-based composite technology. This study investigated the physical and mechanical properties and the effects of the airflow resistivity of the materials and the depth of the cavities behind on sound-absorbing performance of the composite material. The results showed that the composite of best physical and mechanical properties and sound absorption was made with a 150°C hot-pressing temperature, 10 min hot-press time, 3: 1 the ratio of wood fiber to polyester fiber, foaming agent content of 8%, and the nominal density of 0.2 g/cm3; the composite material yielded superior sound absorption property with the airflow resistivity of 1.98 × 105 Pa·s/m2; the acoustic absorption peak moved to lower frequencies when length of the cavities was increased. © 2015 Limin Peng et al. Source

Li Q.F.,Chinese Academy of Forestry | Li Q.F.,GuangXi Zhuang Autonomous Region Forestry Research Institute | Wang J.H.,Chinese Academy of Forestry | Pulkkinen P.,Finnish Forest Research Institute | Kong L.S.,University of Victoria
PLoS ONE | Year: 2015

Embryogenic cultures of Picea balfouriana, which is an important commercial species for reforestation in Southern China, easily lose their embryogenic ability during long-term culture. Embryogenic tissue that proliferated at lower concentrations (3.6 μM and 2.5 μM) of 6- benzylaminopurine (6-BAP) were more productive, and generated 113 ± 6 and 89 ± 3 mature embryos per 100 mg embryogenic tissue, respectively. A metabolomic approach was used to study the changes in metabolites linked to embryogenic competence related to three different 6-BAP concentrations (2.5 μM, 3.6 μM, and 5 μM). A total of 309 compounds were obtained, among which 123 metabolites mapped to Kyoto Encyclopedia of Genes and genomes (KEGG) pathways. The levels of 35 metabolites were significantly differentially regulated among the three 6-BAP treatments, and 32 metabolites differed between the 2.5 μM and 5 μM treatments. A total of 17 metabolites appeared only once among the three comparisons. The combination of a score plot and a loading plot showed that in the samples with higher embryogenic ability (3.6 μM and 2.5 μM), up-regulated metabolites were mostly amino acids and down-regulated metabolites were mostly primary carbohydrates (especially sugars). These results suggested that 6-BAP may influence embryogenic competence by nitrogen metabolism, which could cause an increase in amino acid levels and higher amounts of aspartate, isoleucine, and leucine in tissues with higher embryogenic ability. Furthermore, we speculated that 6-BAP may affect the amount of tryptophan in tissues, which would change the indole-3-acetic acid levels and influence the embryogenic ability. © 2015 Li et al.This is an open access article distributed under the terms of the Creative Commons Attribution License ,which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source

Cai J.,Tianjin University of Science and Technology | Wu Q.,GuangXi Zhuang Autonomous Region Forestry Research Institute | Wang G.,Tianjin University of Science and Technology | Wang G.,CAS Qingdao Institute of Oceanology | Deng C.,National Marine Environmental Monitoring Center
Renewable Energy | Year: 2013

The fermentative hydrogen production capability of the newly isolated Clostridium sp. 6A-5 bacterium was studied in a batch cultivation experiment. Various culture conditions (temperature, initial pH, and glucose concentration) were evaluated for their effects on cell growth and hydrogen production (including the yield and rate) of Clostridium sp. 6A-5. Optimal cell growth was observed at 40°C, initial pH 7.5-8, and glucose concentration 16-26g/L. The optimal hydrogen yield was obtained at 43°C, initial pH 8, and glucose concentration 10-16g/L. Hydrogen began to evolve when cell growth entered the mid-exponential phase and reached the maximum production rate at the late exponential and stationary phases. The maximum hydrogen yield, and rate were 2727mL/L, and 269.3mL H2/L h, respectively. These results indicate that Clostridium sp. 6A-5 is a good candidate for mesophilic fermentative hydrogen production. © 2013. Source

Li Y.,Zhengzhou University | Li Y.,Henan Institute of Science and Technology | Yang J.,Henan Institute of Science and Technology | Zhou X.,Henan Institute of Science and Technology | And 3 more authors.
Applied Biochemistry and Biotechnology | Year: 2014

Endophytic fungi of inner root bark of Taxus wallichiana var. mairei were investigated in order to find endophytes producing 10-DABIII (10-deacetyl baccatin III). Purified colonies were cultured in potato dextrose broth (PDB), and then the organic extracts from fungi were analyzed with HPLC, LC-MS, and 1H NMR. Of 102 fungal endophytes isolated from the inner root bark, only one strain named IRB54 can yield 10-DABIII but no taxol and baccatin III. In PDB culture medium, its productivity was 187.564 ug/l. Based on its morphological characteristics and molecular data, the IRB54 strain was identified as Trichoderma sp. The isolation of the fungus IRB54 yielding 10-DABIII will provide an alternative resource to manufacture taxol/taxotere via semi-synthesis and some useful clues for improving the understanding of taxane synthesis evolution. © 2014, Springer Science+Business Media New York. Source

Hu Y.,Guangxi University | Chen H.,GuangXi Zhuang Autonomous Region Forestry Research Institute | Luo C.,Guangxi University | Dong L.,Guangxi University | And 4 more authors.
Scientia Horticulturae | Year: 2014

The proper selection of stable reference genes for real-time quantitative PCR (RT-qPCR) can help to reliably assess gene expression results under specific experimental conditions. The present study evaluated the stability of 16 candidate reference genes of kumquat using the methods of geNorm, Normfinder, and Bestkeeper in different tissues of kumquat (Fortunella crassifolia Swingle) under some type of abiotic stress. The results showed that homologous genes should be taken into account for normalization in RT-qPCR studies. For the three specific types of stresses, i.e., salt stress, drought stress, and heavy metal stress, ACT6, ACT8, and ACT7 should be selected as internal controls, respectively. For low-temperature stress, the use of multiple reference genes for normalization may be optimal, such as ACT7+. ACT9 (according to geNorm) or ACT7+. TUB4 (according to Normfinder). We suggest that ACT7 alone or the combination of ACT7+. ACT1+. EF1-α or ACT1+. ACT8 can meet the requirements for the normalization of RT-qPCR studies under the experimental conditions tested here. Although some commonly used reference genes (TUA1, TUB1, TUB4, CYP, GAPDH, etc.) showed much higher variability, some are still useful in combinations under specific conditions. © 2014. Source

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