Guangxi Traditional Chinese Medical University

www.gxtcmu.edu.cn
Nanning, China
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Xie J.-L.,Guangxi Traditional Chinese Medical University | Zhao C.,Guangxi Traditional Chinese Medical University | Li J.-X.,Guangxi Traditional Chinese Medical University | Wei Y.-F.,Guangxi Traditional Chinese Medical University
Chinese Pharmacological Bulletin | Year: 2016

Aim: To investigate the effect of plumbagin on invasion and apoptosis of human hepatocellular carcinoma (HepG2). Methods: HepG2 cells were cultured in vitro with different concentrations of plumbagin, then cell proliferation was observed by MTT assay; cell invasion was observed by transwell invasion assay; cell apoptosis was detected by flow cytometry, and the protein expression of Bax, Bel-2 was detected by immunocytochemistry. Results: MIT results showed that plumbagin could significantly inhibit cell proliferation compared with the control group, and in a dose-dependent manner (P < 0. 05). Transwell invasion assay showed that cell invasion was significantly decreased with increasing concentrations of plumbagin (P < 0. 01). Flow cytometry showed that apoptosis rate was significantly higher in 4, 8 μmol · L-1 group of plumbagin compared with that of the control group (P<0. 01). Immunocytochemistry showed that, with the increasing concentration of plumbagin, Bax protein expression increased, Bcl-2 protein expression was decreased, both in a dose-dependent manner (P < 0.01). Conclusion: Plumbagin can inhibit HepG2 cell proliferation and accelerate apoptosis of HepG2 cells, but also has the ability to inhibit HepG2 cell invasion.


Wei W.,Guangxi University | Li X.,Guangxi University | Wang K.,Zhejiang GongShang University | Zheng Z.,Guangxi Traditional Chinese Medical University | Zhou M.,Guangxi University
Phytotherapy Research | Year: 2012

One new lignan, nirtetralin B, along with its two known stereoisomers were isolated from Phyllanthus niruri L. The structure of the new compound was determined by spectroscopy experiments and x-ray diffraction analysis. These lignans were assayed for anti-hepatitis B virus activities in vitro. Nirtetralin and nirtetralin A, B effectively suppressed the secretion of the HBV antigens in a dose-dependent manner with IC 50 values for HBsAg of 9.5 μm (nirtetralin A), 16.7 μm (nirtetralin B) and 97.2 μm (nirtetralin), IC 50 values for HBeAg of 17.4 μm (nirtetralin A), 69.3 μm (nirtetralin B) and 232.0 μm (nirtetralin), respectively. Copyright © 2011 John Wiley & Sons, Ltd.


Liu X.,Guangxi Traditional Chinese Medical University | Liang J.,Guangxi Traditional Chinese Medical University | Li G.,Sun Yat Sen University
Molecular Biology Reports | Year: 2010

Inflammation and infection have been linked to the bionomics of many cancers, including hepatocellular carcinoma (HCC). Some bacteria, such as Helicobacter pylori, have been found in pathological specimens from patients with HCC. However, little is known about the direct effects of bacteria or their components on hepatoma cells. We analyzed the in vitro proliferation, adhesion and invasion responses of the tumor cell lines HepG2 and HepG2.2.15 to lipopolysaccharide (LPS), a cell wall constituent of Gram-negative bacteria. HepG2 and HepG2.2.15 cells show increased proliferation in response to LPS. Their invasion and adhesion abilities were also increased in response to LPS, which may be related to increased gene expression of interleukin-8 and transforming growth factor-β1. We infer that bacteria may be ignored by immune systems and directly promote adhesion and invasion of hepatoma cells through LPS. © 2009 Springer Science+Business Media B.V.


Ansheng M.,Guangxi Traditional Chinese Medical University
Chinese Journal of Traumatology - English Edition | Year: 2014

Traumatic arteriovenous fistula between the axillary artery and vein may present a difficult problem in treatment. There are few reports demonstrating the endovascular repair of this challenge. Herein, we present such a case of endovascular repair of traumatic arteriovenous fistula between the axillary artery and vein with false aneurysm formation. The patient was discharged 11 days after successful operation. Oral clopidogrel and aspirin were administerted for 18 months. At one year follow-up, the patient was in good condition and showed no evidence of neurological deficit in the left upper limb. © 2014 Daping hospital and the Research Institute of Surgery of the Third Military Medical University.


Qin B.,Guangxi Traditional Chinese Medical University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To determine curcumin content by HPLC and observe the antitumor effects in vivo from different processed products of Curuma kwangsiensis. We determined the content of curcumin by HPLC from different processed products of Curuma kwangsiensis and studied the anti-tumor effects in vivo by establishing S180 transplanted tumors model in mice. The content of curcumin in different processed products of Curuma kwangsiensis as follows: primitive > vinegar-fried > vinegar-cooked. Different processed products had anti-tumor effects in the tumor-bearing mice. The vinegar-fried group and vinegar-cooked group showed obvious effect of inhibiting tumor growth than the primitive group. Different processed methods may have some affection to the quality of Curuma kwangsiensis.


Huang J.L.,Guangxi Traditional Chinese Medical University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

To explore the mechanism of apoptosis of human hepatocellular carcinoma cells BEL-7404 induced by Galic acid extracted from leaves of Phyllanthus emblica. PI/Hoechst33342 double staining method was utilized to observe the influence on cell life cycle. The expressions of Bax and Bcl-2 protein were determined by fluorescence immunostaining and Western blot. The results from PI/Hoechst33342 double staining method indicated that the percentage of cells G2/M phases increased after treated for 72 h, the phenomenon of blockage appeared and cell death was induced. Fluorescence immunostaining showed that the expression of Bax was up-regulated, and the expression of Bcl-2 was down-regulated. Western blot also showed that the the expression of Bax was up-regulated. The mechanism of human hepatoma BEL-7404 cells apoptosis induced by Galic acid from leaves of Phyllanthus emblica may be blocking G2/M period in cell life cycle, up-regulating the expression of Bax and down-regulating the expression of Bcl-2, that can decrease membrane potential of mitochondria,and triggered the caspases of activation of cascade and induced cell death.


Zhong Z.G.,Guangxi Traditional Chinese Medical University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

To study the effect of Panax notoginseng saponins (PNS) on learning and memory ability and APP gene transcription in the brain tissue in senescence accelerated mouse prone 8 (SAMP8). SAMP8 were randomly divided into high-does PNS group, low-does PNS group, huperzin A group and model group,the treatment groups were treated with the designed drugs respectively by intragastric administration for 4 consecutive weeks. The same volume of double distilled water was given to model group. After treatment, the abilities of learning and memory of the mice were tested with morris water maze, the mRNA content of APP was assayed by reverse transcription (RT) and real-time polymerase chain reaction (RT-PCR). PNS could improve the abilities of learning and memory, high-does PNS could reduce the mRNA content of APP in the brain tissue of SAMP8. PNS can improve the abilities of learning and memory of SAMP8, the mechanism may be relevant to down-regulating the expression of APP gene at transcriptional level.


Yang Z.M.,Guangxi Traditional Chinese Medical University
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica | Year: 2012

To study the inhibitory effect of total saponins of the root and rhizome of Panax notoginseng (PNS) on drug metabolism enzyme CYP3A in rat livers and its kinetic analysis. Microsome enzyme was prepared by differential velocity centrifugation. Michaelis constant (Km) and maximum velocity (Vmax) of CYP3A, 50% inhibitory concentration of PNS on CYP3A, and the inhibition type and the inhibition constant of CYP3A (Ki, Kis) of PNS on CYP3A were calculated by Lineweaver-Burk and the low of semi-effect-probit. Total saponins of the root and rhizome of panax notoginseng inhibited CYP3A activity, with IC50 of 689.54 mg x L(-1). Compared with the substrate aminopyrine, CYP3A showed Km of 0.036 mmol x L(-1) and Vmax of 21.01 micromol min(-1) x g(-1). Total saponins of the root and rhizome of panax notoginseng showed a mixed inhibition on CYP3A, with the inhibition constants of 247.79 mg x L(-1) (Ki) and 321.79 mg x L(-1) (Kis). Total saponins of the root and rhizome of panax notoginseng have a significant effect on CYP3A activity in rat livers.


Liu X.M.,Guangxi Traditional Chinese Medical University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2013

To investigate the effect of plumbagin on the expression of TNF-alpha and PDGF-BB in human hepatic stellate cells (HSC-LX2) activated by Leptin. HSC-LX2 were cultured in vitro and stimulated by Leptin for 24 hours then treated with different concentrations of plumbagin for 24 hours, the expressions of TNF-alpha mRNA and PDGF-BB mRNA were determined by Realtime quantitative PCR, the protein expressions of TNF-alpha and PDGF-BB were determined-by Western blotting. The expressions of TNF-alpha mRNA and PDGF-BB mRNA of treatment groups were significantly reduced, especially in high dose group (P < 0.01), and Western blotting analyses revealed similar trends in protein expression. Plumbagin may prevent the formation of hepatic fibrosis and its mechanism may be related to decreasing the level of mRNA of TNF-alpha and PDGF-BB and the protein of PDGF-BB.


Qiu Q.,Guangxi Traditional Chinese Medical University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2013

To study the chemical constituents of petroleum ether portion from Seed of Picria felterrae. The chemical constituents were extracted and isolated with solvent and chromatography, and the chemical structures were determined on the basis of physicochemical properties and spectral data (UV, IR, 1H-NMR, 13C-NMR, MS). Six chemical constituents were identified as follows: chrysophanol (I), 1-hydroxy-6-methyl-anthraquinone (II), beta-sitosterol (III), betulin (IV), N-benzoyl-phenylalanyl-L-phenylalaninol acetate (V) and picfeltarraenone I (VI). Compounds I, II and IV are isolated from this plant for the first time.

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