Guangxi Traditional Chinese Medical University

www.gxtcmu.edu.cn
Nanning, China

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Su Q.,Guangxi Traditional Chinese Medical University | Liang H.,Guangxi Medical University | Cen P.,Centers for Disease Control and Prevention | Bi Z.,Centers for Disease Control and Prevention | Zhou P.,Centers for Disease Control and Prevention
AIDS Research and Human Retroviruses | Year: 2012

We investigated the distribution of HIV-1 subtypes and the prevalence of HIV-1 drug resistance among HIV-infected antiretroviral-naive patients in Guangxi, southern China. A total of 144 subjects from Liuzhou or Nanning, two cities having the most HIV-infected cases in Guangxi, were enrolled. HIV-1 pol fragments were amplified and sequenced from plasma of all patients. In all, 124 sequences were obtained, with 67 from Liuzhou and 57 from Nanning. All sequences were subtyped by phylogenetic analysis and analyzed for antiretroviral resistance using the HIVdb program. Our data showed that the sequences from Liuzhou were subtyped as CRF01-AE (77.6%), CRF07-BC(20.9%), and BC (1.5%), respectively, and the sequences from Nanning as CRF01-AE (78.9%), CRF08-BC (15.8%), B (3.5%), and C (1.8%), respectively. Of the sequences 11.9% from Liuzhou and 28.1% from Nanning harbored drug resistance-associated mutations, but there were only two sequences with mutations associated with significantly reduced phenotypic susceptibility to antiretroviral drugs. © Copyright 2012, Mary Ann Liebert, Inc. 2012.


Liu X.,Guangxi Traditional Chinese Medical University | Liang J.,Guangxi Traditional Chinese Medical University | Li G.,Sun Yat Sen University
Molecular Biology Reports | Year: 2010

Inflammation and infection have been linked to the bionomics of many cancers, including hepatocellular carcinoma (HCC). Some bacteria, such as Helicobacter pylori, have been found in pathological specimens from patients with HCC. However, little is known about the direct effects of bacteria or their components on hepatoma cells. We analyzed the in vitro proliferation, adhesion and invasion responses of the tumor cell lines HepG2 and HepG2.2.15 to lipopolysaccharide (LPS), a cell wall constituent of Gram-negative bacteria. HepG2 and HepG2.2.15 cells show increased proliferation in response to LPS. Their invasion and adhesion abilities were also increased in response to LPS, which may be related to increased gene expression of interleukin-8 and transforming growth factor-β1. We infer that bacteria may be ignored by immune systems and directly promote adhesion and invasion of hepatoma cells through LPS. © 2009 Springer Science+Business Media B.V.


Ansheng M.,Guangxi Traditional Chinese Medical University
Chinese Journal of Traumatology - English Edition | Year: 2014

Traumatic arteriovenous fistula between the axillary artery and vein may present a difficult problem in treatment. There are few reports demonstrating the endovascular repair of this challenge. Herein, we present such a case of endovascular repair of traumatic arteriovenous fistula between the axillary artery and vein with false aneurysm formation. The patient was discharged 11 days after successful operation. Oral clopidogrel and aspirin were administerted for 18 months. At one year follow-up, the patient was in good condition and showed no evidence of neurological deficit in the left upper limb. © 2014 Daping hospital and the Research Institute of Surgery of the Third Military Medical University.


Qin B.,Guangxi Traditional Chinese Medical University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To determine curcumin content by HPLC and observe the antitumor effects in vivo from different processed products of Curuma kwangsiensis. We determined the content of curcumin by HPLC from different processed products of Curuma kwangsiensis and studied the anti-tumor effects in vivo by establishing S180 transplanted tumors model in mice. The content of curcumin in different processed products of Curuma kwangsiensis as follows: primitive > vinegar-fried > vinegar-cooked. Different processed products had anti-tumor effects in the tumor-bearing mice. The vinegar-fried group and vinegar-cooked group showed obvious effect of inhibiting tumor growth than the primitive group. Different processed methods may have some affection to the quality of Curuma kwangsiensis.


Huang J.L.,Guangxi Traditional Chinese Medical University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

To explore the mechanism of apoptosis of human hepatocellular carcinoma cells BEL-7404 induced by Galic acid extracted from leaves of Phyllanthus emblica. PI/Hoechst33342 double staining method was utilized to observe the influence on cell life cycle. The expressions of Bax and Bcl-2 protein were determined by fluorescence immunostaining and Western blot. The results from PI/Hoechst33342 double staining method indicated that the percentage of cells G2/M phases increased after treated for 72 h, the phenomenon of blockage appeared and cell death was induced. Fluorescence immunostaining showed that the expression of Bax was up-regulated, and the expression of Bcl-2 was down-regulated. Western blot also showed that the the expression of Bax was up-regulated. The mechanism of human hepatoma BEL-7404 cells apoptosis induced by Galic acid from leaves of Phyllanthus emblica may be blocking G2/M period in cell life cycle, up-regulating the expression of Bax and down-regulating the expression of Bcl-2, that can decrease membrane potential of mitochondria,and triggered the caspases of activation of cascade and induced cell death.


Liang J.,Guangxi Traditional Chinese Medical University | Jiang M.J.,Guangxi Traditional Chinese Medical University | Deng X.,Guangxi Traditional Chinese Medical University | Xiao Zhou X.,Guangxi Traditional Chinese Medical University
Hepatitis Monthly | Year: 2013

Background: Hepatitis B virus (HBV) infection is a serious global health problem that is associated with huge social and economic costs. Early antiviral drugs, such as interferon-α2b, peginterferon-α2a, lamivudine, and adefovir, all have their limitations (such as low responses or safety concerns) in clinical application. Telbivudine and entecavir are two of the latest nucleotide drugs and both have been shown to have potent viral suppression. However, in patients with hepatitis B e antigen (HBeAg)-positive chronic hepatitis B (CHB), inconsistent results have been generated for efficacy between telbivudine and entecavir. Therefore, evidence-based medical data are required to compare the efficacies, in terms of virological and biochemical responses, and safety between telbivudine and entecavir. Objectives: We aimed to compare the early antiviral efficacy and safety of telbivudine and entecavir in the treatment of patients with hepatitis B e antigen (HBeAg)-positive chronic hepatitis B (CHB). Patients and Methods: A search for relevant randomized controlled trials (RCTs) on HBeAg-positive CHB patients treated with telbivudine and entecavir for 24 or 52 weeks, published before December 2011, was performed. Primary efficacy endpoint was the cumulative rate of undetectable HBV DNA, and secondary efficacy endpoints included rates of alanine aminotransferase (ALT) normalization, HBeAg disappearance, HBeAg seroconversion and adverse events. Meta-analysis was performed using the Review Manager v5.1.4 software package. We assessed the pooled risk ratios (RRs) and 95% confidence intervals (CIs) using the fixed-or random-effects model. Results: Six randomized controlled trials (RCTs) involving 555 patients were included. Telbivudine was associated with significantly higher rates of HBeAg disappearance (RR = 1.46, 95% CI: 1.11 - 1.91) and HBeAg seroconversion (RR = 1.76, 95%CI: 1.25-2.48) than entecavir, but had higher adverse events (RR = 2.11, 95%CI: 1.23 - 3.60), compared with entecavir. There was no difference between telbivudine and entecavir in the rate of cumulative undetectable HBV DNA (RR = 0.99, 95% CI: 0.90 - 1.10) and ALT normalization (RR = 0.93, 95% CI: 0.85 - 1.00). Conclusions: Telbivudine is associated with significantly higher rates of HBeAg disappearance and HBeAg seroconversion than entecavir, whereas entecavir is superior to telbivudine in safety. Both drugs have similar efficacy on rates of cumulative undetectable HBV DNA and ALT normalization. © 2013, Kowsar Corp.


Zhong Z.G.,Guangxi Traditional Chinese Medical University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

To study the effect of Panax notoginseng saponins (PNS) on learning and memory ability and APP gene transcription in the brain tissue in senescence accelerated mouse prone 8 (SAMP8). SAMP8 were randomly divided into high-does PNS group, low-does PNS group, huperzin A group and model group,the treatment groups were treated with the designed drugs respectively by intragastric administration for 4 consecutive weeks. The same volume of double distilled water was given to model group. After treatment, the abilities of learning and memory of the mice were tested with morris water maze, the mRNA content of APP was assayed by reverse transcription (RT) and real-time polymerase chain reaction (RT-PCR). PNS could improve the abilities of learning and memory, high-does PNS could reduce the mRNA content of APP in the brain tissue of SAMP8. PNS can improve the abilities of learning and memory of SAMP8, the mechanism may be relevant to down-regulating the expression of APP gene at transcriptional level.


Yang Z.M.,Guangxi Traditional Chinese Medical University
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica | Year: 2012

To study the inhibitory effect of total saponins of the root and rhizome of Panax notoginseng (PNS) on drug metabolism enzyme CYP3A in rat livers and its kinetic analysis. Microsome enzyme was prepared by differential velocity centrifugation. Michaelis constant (Km) and maximum velocity (Vmax) of CYP3A, 50% inhibitory concentration of PNS on CYP3A, and the inhibition type and the inhibition constant of CYP3A (Ki, Kis) of PNS on CYP3A were calculated by Lineweaver-Burk and the low of semi-effect-probit. Total saponins of the root and rhizome of panax notoginseng inhibited CYP3A activity, with IC50 of 689.54 mg x L(-1). Compared with the substrate aminopyrine, CYP3A showed Km of 0.036 mmol x L(-1) and Vmax of 21.01 micromol min(-1) x g(-1). Total saponins of the root and rhizome of panax notoginseng showed a mixed inhibition on CYP3A, with the inhibition constants of 247.79 mg x L(-1) (Ki) and 321.79 mg x L(-1) (Kis). Total saponins of the root and rhizome of panax notoginseng have a significant effect on CYP3A activity in rat livers.


Liu X.M.,Guangxi Traditional Chinese Medical University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2013

To investigate the effect of plumbagin on the expression of TNF-alpha and PDGF-BB in human hepatic stellate cells (HSC-LX2) activated by Leptin. HSC-LX2 were cultured in vitro and stimulated by Leptin for 24 hours then treated with different concentrations of plumbagin for 24 hours, the expressions of TNF-alpha mRNA and PDGF-BB mRNA were determined by Realtime quantitative PCR, the protein expressions of TNF-alpha and PDGF-BB were determined-by Western blotting. The expressions of TNF-alpha mRNA and PDGF-BB mRNA of treatment groups were significantly reduced, especially in high dose group (P < 0.01), and Western blotting analyses revealed similar trends in protein expression. Plumbagin may prevent the formation of hepatic fibrosis and its mechanism may be related to decreasing the level of mRNA of TNF-alpha and PDGF-BB and the protein of PDGF-BB.


Qiu Q.,Guangxi Traditional Chinese Medical University
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2013

To study the chemical constituents of petroleum ether portion from Seed of Picria felterrae. The chemical constituents were extracted and isolated with solvent and chromatography, and the chemical structures were determined on the basis of physicochemical properties and spectral data (UV, IR, 1H-NMR, 13C-NMR, MS). Six chemical constituents were identified as follows: chrysophanol (I), 1-hydroxy-6-methyl-anthraquinone (II), beta-sitosterol (III), betulin (IV), N-benzoyl-phenylalanyl-L-phenylalaninol acetate (V) and picfeltarraenone I (VI). Compounds I, II and IV are isolated from this plant for the first time.

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