Guangxi Subtropical Crops Research Institute

Guangxi, China

Guangxi Subtropical Crops Research Institute

Guangxi, China
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Yang X.-Y.,Guangxi Subtropical Crops Research Institute | Cai Y.-B.,Guangxi Subtropical Crops Research Institute | Zhang Z.-L.,Hainan Academy of Agricultural science | Sun G.-M.,Chinese Academy of Sciences | And 3 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2016

A new cDNA sequence encoding MADS-box protein, designated as AcMADS2 (GenBank accession number: KC257409), from Ananas comosus was cloned by RT-PCR and RACE techniques. The full length cDNA of AcMADS2 has 945 bp nucleotides with an ORF of 687 bp encoding a precursor protein of 228 amino acid residues. Sequence analysis and evolution analysis indicated that the deduced protein contained conservative MADS-box domain and semi-conservative K domain, and shared highly homology to AGL12 clade of MADS-box proteins from other plants. Bioinformatics analysis results demonstrated that AcMADS2 was an unstable and hydrophilic protein without transmembrane region and signal peptide; α-helix, extended strand and random coil were the backbone of its secondary structure; the protein core structure with MADS-box domain in predictable protein 3D model; and might be located in the nucleus as a transcription factor. Real-time quantitative PCR analysis showed that the expression of AcMADS2 gene was much stronger in roots than in florets, stems and leaves at flowering stage, and the adversity stresses such as low temperature and drought could trigger a significant induction of AcMADS2 gene in roots at seedling stage. All these results suggested that the AcMADS2 protein may be involved in the regulation of root development, and play an important role in responses to adversity stresses. © 2016, Science Press. All right reserved.


Cai L.,State Key Laboratory of Agricultural Microbiology | Cai L.,Huazhong Agricultural University | Zhang Y.C.,State Key Laboratory of Agricultural Microbiology | Zhang Y.C.,Huazhong Agricultural University | And 5 more authors.
Plant Disease | Year: 2017

Nonheading Chinese cabbage (Brassica rapa L. var. chinensis [Linnaeus] Kitamura) is a variant of Chinese cabbage. It is characterized by well-developed edible flower stalks. This vegetable has been cultivated for over 1,000 years in China, mostly in the Yangtze River region. Nowadays, it has become globalized with cultivation in Southeast Asia, Europe, and America (Hou 2003). Recently, a general virus-like symptom of mosaic leaf had been observed on this plant, with a high incidence ranging from 16 to 30% in the field. From March 2010 to December 2016, the incidence of Broad bean wilt virus 2 (BBWV2, genus Fabavirus, family Secoviridae), together with Turnip mosaic virus (TuMV, genus Potyvirus, family Potyviridae) and Cucumber mosaic virus (CMV, genus Cucumovirus, family Bromoviridae), commonly infecting brassica vegetable crops in nonheading Chinese cabbage was investigated in Wuhan, Hubei Province, China. Seventy-nine leaf samples showing mosaic symptoms were randomly collected and tested by dot enzyme-linked immunosorbent assay (Dot-ELISA) with BBWV2 polyclonal antibody (Agdia, Elkhart, IN) described by Pappas et al. (1983). The results showed that all samples were positive for at least one of the viruses, 41 positive for two or three viruses, and 29 positive (36.7%) for BBWV2. Five samples positive for BBWV2 were subjected to total RNA extraction using TRIzol reagent following the manufacturer’s instruction (Invitrogen, U.S.A.), and further identified by RT-PCR using the primer set BBWV-F/-R (5′-AGAAAYAGGAAGGTGCGTGC-3′/5′-TCTRTTGCACATGGCATACC-3′; Y = C/T, R = C/G). A DNA fragment of approximately 1,660-bp was amplified from the five samples. One of the amplificons was sequenced (GenBank accession no. KY886225) in Sangon Biotech Co., Ltd., Shanghai, China. BLASTn analyses revealed that this isolate shared the highest identity of 95.0% with a tomato isolate XJ14-3 of BBWV2 (FN985164) from China. To our knowledge, this is the first report of BBWV2 infecting nonheading Chinese cabbage (Edwardson and Christie 1991). Our results indicate that nonheading Chinese cabbage plants are widely infected by BBWV2. The crop may serve as an overwinter host, posing a potential risk for other host crops, e.g., tomato (Solanum lycopersicum), broad bean (Vicia faba), pepper (Capsicum annuum), eggplant (S. melongena), and kidney bean (Phaseolus vulgaris), in coming season. © 2017, American Phytopathological Society. All rights reserved.


Cai L.,State Key Laboratory of Agricultural Microbiology | Cai L.,Huazhong Agricultural University | Zhang Y.C.,State Key Laboratory of Agricultural Microbiology | Zhang Y.C.,Huazhong Agricultural University | And 5 more authors.
Plant Disease | Year: 2017

Purple Cai-tai (Brassica campestris L. var. purpuraria L. H. Bailey) is one of the most popular vegetable crops in the south of China, and has been cultivated over thousands of years. From March 2010 to December 2016, the incidence of Cucumber mosaic virus (CMV, genus Cucumovirus, family Bromoviridae) in purple Cai-tai was investigated in Wuhan, Hubei Province, China. Leaf samples (n = 185) of purple Cai-tai, showing leaf mosaic, leaf shrinking, and dwarf symptoms, were randomly collected, and subjected to Dot-ELISA with CMV polyclonal antibody (Agdia, Elkhart, IN) (Pappas et al. 1983). The results indicated that 50 samples were positive for CMV, accounting for a 27.0% incidence of the collected samples. Twenty samples positive for CMV indexed by Dot-ELISA were further subjected to total RNA extraction using TRIzol reagent (Invitrogen, U.S.A.) following the manufacturer’s manual, and identified by RT-PCR amplification using the primer set CMV-1/-2 (5′-ATGGACAAATCTGRATCWMCC-3′/5′-CTGGATGGACAACCCGTTC-3′; R=A or G, W=A or T, M=A or C) (Xi et al. 2006), generating fragments ranging from 773 to 788 bp (containing the 657-bp capsid protein [CP] gene and partial 3′ noncoding region). The amplified products were cloned into pGEM-T (Promega, Madison, WI), and eight clones (termed CMV-PCT1 to CMV-PCT8 under GenBank accession nos. MF038797–99 and MF038792–96) were randomly chosen for sequencing in Sangon Biotech Co., Ltd. (Shanghai, China). Phylogenetic analysis of the CP gene nucleotide sequences by Mega 5.0 using the maximum likelihood method revealed that these CMV isolates tentatively fell into subgroup IB (CMV-PCT4 to -8) and II (CMV-PCT1 to -3), delimitated by Roossinck et al. (1999), respectively. To our knowledge, this is the first report of CMV infecting purple Cai-tai, extending the known host range of CMV (Phan et al. 2014). Whether the symptoms observed in the field were induced by the CMV infection needs further study. Our results indicated that purple Cai-tai plants were widely infected by CMV, and posed a high risk as potential virus reservoir. As purple Cai-tai plants are traditionally grown from autumn through spring, Cai-tai crops can provide a living host for CMV to overwinter in the absence of other crops. Our studies also provide important information for further improving the certification program of purple Cai-tai propagation materials in China. © 2017, American Phytopathological Society. All rights reserved.


Cheng H.,Chinese Academy of Sciences | Cai H.,Chinese Academy of Sciences | Fu H.,Guangxi Subtropical Crops Research Institute | An Z.,Chinese Academy of Sciences | And 4 more authors.
PLoS ONE | Year: 2015

Rubber trees (Hevea brasiliensis) are susceptible to low temperature and therefore are only planted in the tropical regions. In the past few decades, although rubber trees have been successfully planted in the northern margin of tropical area in China, they suffered from cold injury during the winter. To understand the physiological response under cold stress, we isolated a C-repeat binding factor 1 (CBF1) gene from the rubber tree. This gene (HbCBF1) was found to respond to cold stress but not drought or ABA stress. The corresponding HbCBF1 protein showed CRT/DRE binding activity in gel shift experiment. To further characterize its molecular function, the HbCBF1 gene was overexpressed in Arabidopsis. The HbCBF1 over expression (OE) line showed enhanced cold resistance and relatively slow dehydration, and the expression of Arabidopsis CBF pathway downstream target genes, e.g. AtCOR15a and AtRD29a, were significantly activated under non-acclimation condition. These data suggest HbCBF1 gene is a functional member of the CBF gene family, and may play important regulation function in rubber tree. © 2015 Cheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Xie H.,Guangxi University | Xie H.,Guangxi Subtropical Crops Research Institute | Wei J.,Guangxi University | Huang R.,Guangxi University | Yang X.B.,Iowa State University
Mycology | Year: 2016

Genetic diversity of 23 Lasiodiplodia theobromae isolates on Morus alba and 6 isolates on Agave sisalana in Guangxi province, China, was studied by using random amplified polymorphic DNA and inter-simple sequence repeat molecular markers. Results of two molecular markers showed that the average percentage of polymorphic loci of all isolates was more than 93%. Both dendrograms of two molecular markers showed obvious relationship between groups and the geographical locations where those strains were collected, among which, the 23 isolates on M. alba were divided into 4 populations and the 6 isolates on A. sisalana were separated as a independent population. The average genetic identity and genetic distance of 5 populations were 0.7215, 0.3284 and 0.7915, 0.2347, respectively, which indicated that the genetic identity was high and the genetic distance was short in the 5 populations. Average value of the gene diversity index (H) and the Shannon’s information index (I) of 29 isolates were significantly higher than 5 populations which showed that genetic diversity of those isolates was richer than the populations and the degree of genetic differentiation of the isolates was higher. The Gst and Nm of 29 isolates were 0.4411, 0.6335 and 0.4756, 0.5513, respectively, which showed that the genetic diversity was rich in those isolates. © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.


Jiang J.,State Key Laboratory of Agricultural Microbiology | Jiang J.,Huazhong Agricultural University | Jiang J.,Key Laboratory of Plant Pathology of Hubei Province | Zhai H.,State Key Laboratory of Agricultural Microbiology | And 21 more authors.
Crop Protection | Year: 2014

In recent years, Pyrus bretschneideri Rehd. var. 'Suli' has been damaged by a disease characterized by the presence of black spots on young fruit in China, which was always followed by severe bitter rot on matured fruits. The etiology of these symptoms and their relationship with the bitter rot was unknown. A colony was routinely isolated from young and matured 'Suli' pear fruits showing black spots and rot symptoms, respectively. This fungal colony was identified as Colletotrichum fructicola based on morphological characteristics and DNA sequence data of 7 regions. C. fructicola was confirmed to be capable of eliciting both black spots and bitter rot symptoms by completion of Koch's Postulates. The results suggested that the disease characterized by the presence of black spots on young fruits is indeed an early stage of the bitter rot observed on matured fruits of 'Suli' pear. Variety resistance analysis indicated an obvious resistance variation possessed by the pear varieties generally cultured in China, with black spots only occurring on some pear varieties. Our results also indicated that wounding can break the quiescent infections, enhance the infectivity of C.fructicola, and lead to more rapid rot of young and matured fruits. These results provided a clear clue that C.fructicola can invade some varieties of P.bretschneideri Rehd. directly without entry via wounds, but subsequently turned into quiescent infection and causing black spots on the fruit surface. The quiescent infection acts as a pathogenic factor responsible for bitter rot of matured fruits. The influence of temperature and pH conditions for growth and colony morphology of C.fructicola were also evaluated. The characterization of C.fructicola causing bitter rot of pear (P.bretschneideri) is expected to provide useful information for controlling this economically important disease. © 2014 Elsevier Ltd.


Chen T.,Guangxi Subtropical Crops Research Institute | Qin J.,Guangxi Subtropical Crops Research Institute | Xu S.,Guangxi Subtropical Crops Research Institute | Huang M.,Guangxi Subtropical Crops Research Institute | And 3 more authors.
Research on Crops | Year: 2013

No-tillage (NT) often leads to decreased root growth in irrigated rice at early growth stage. This study was conducted to determine NT effects on rhizosphere soil nutrient contents and root lipid peroxidation and antioxidative properties of irrigated rice and identify whether these effects are responsible for the decreased root growth in NT rice. Pot experiments were conducted in two seasons (early and late). A hybrid rice cultivar was grown under NT and conventional tillage (CT) in each season. Root biomass decreased by 31% in early season and by 34% in late season under NT as compared to under CT. NT had equal or higher available nutrient contents in rhizosphere soil than CT in both the seasons. Root malondialdehyde content was higher under NT than under CT in late season, while the difference was insignificant in early season. Root antioxidative enzyme activities under NT were lower than under CT, and the differences in superoxide dismutase and catalase activities were smaller in late season than in early season. Our study suggests that soil nutrient supply and root oxidative damage are not general factors contributing to the decreased root growth in NT rice, and superoxide dismutase and catalase are sensitive to the oxidative damage in NT rice roots.


Huang S.-S.,Kunming University of Science and Technology | Huang S.-S.,Guangxi Academy of science | Lai J.-Z.,Guangdong Institute of Arts science | Lu M.-Q.,Guangxi Academy of science | And 3 more authors.
Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis | Year: 2015

A modified procedure of Percoll density gradient centrifugation was developed to isolate and fractionate synchronous cells from stationary phase (sp) cultures of different yeast strains, as well as Raman spectra discrimination of single yeast cells was reported. About 1.75 mL Percoll solution in 2 mL polypropylene centrifugal tube was centrifuged at 19320 g, 20℃ with an angle rotor for 15 min to form continuous densities gradient (1.00~1.31 g·mL-1), approximately 100 μL sample was overlaid onto the preformed continuous density gradient carefully, subsequently, centrifuged at 400 g for 60 min in a tabletop centrifuge equipped with a angle rotor at 25℃. Yeast samples could be observed that the suspensions were separated into two cell fractions obviously. Both fractions of different yeast strains were respectively determined by differential interference contrast (DIC), phase contrast microscope and synchronous culture to distinguish their morphological and growth trait. The results showed that the lower fraction cells were unbudded, mostly unicellular, highly refractive, homogeneous and uniform in size, and represented growth characteristic synchronously; Their protoplasm had relatively high density, and contained significant concentrations of glycogen; all of which were accordant with description of quiescent yeast cells and G0 cells in previously published paper. It was shown that lower fraction was quiescent cells, synchronous G0 cells as well. A Raman tweezers setup was used to investigate the differences between two fractions, G0 cells and non G0 cells, at a single cell level. The result showed that both G0 cells and the non G0 cells had the same characteristic peaks corresponding biological macromolecules including proteins, carbohydrates and nucleic acids, but all characteristic peak intensities of G0 cells were higher than that of non G0 cells, implied that the macromolecular substance content of G0 cells was more higher. Principal component analysis (PCA) was performed between G0 cells and non G0 cells, the results showed that the chemical composition content among the synchronization G0 cells has less difference, and G0 cells were homogeneous but non G0 cells were heterogeneous, indicating single cell optical tweezers Raman spectroscopy could identify the synchronous and asynchronous cells. The modified method is feasible, economical and efficient highly. G0 synchronous cells of most yeast strains could be isolated by a modification of Percoll density gradient centrifugation. ©, 2015, Science Press. All right reserved.


Niu F.,Jiangnan University | Zhou J.,Jiangnan University | Zhou J.,Xinjiang Agricultural University | Niu D.,Guangxi Subtropical Crops Research Institute | And 4 more authors.
Food Hydrocolloids | Year: 2015

This study investigated the complexes formed between ovalbumin (OVA) and gum arabic (GA) and their ability to stabilize oil-water emulsions. The stability of the emulsions stabilized by the OVA/GA complexes was evaluated by measuring storage time (1-7 days), salt concentration (0-100mM NaCl), and heat (40-90°C, 30min) stability at pH 3.8-7.0. The results showed that the stability of the OVA-stabilized emulsion was greatly improved by an OVA/GA ratio of 1:2 and acidic pH. Above or below 1:2, the emulsion was unstable due to depletion or bridging flocculation. Emulsions saturated with OVA/GA complexes were stable with changing pH, storage time, and heat treatment (up to 90°C). However, the OVA/GA complexes did not improve the stability of the emulsions when salt concentrations were varied. The experimental results demonstrated that OVA/GA complexes could be used to prepare stable emulsion structures, which may be useful in the beverage industry. © 2015 Elsevier Ltd.


PubMed | Chinese Academy of Sciences, Guangxi Subtropical Crops Research Institute and Chinese University of Hong Kong
Type: Journal Article | Journal: PloS one | Year: 2015

Rubber trees (Hevea brasiliensis) are susceptible to low temperature and therefore are only planted in the tropical regions. In the past few decades, although rubber trees have been successfully planted in the northern margin of tropical area in China, they suffered from cold injury during the winter. To understand the physiological response under cold stress, we isolated a C-repeat binding factor 1 (CBF1) gene from the rubber tree. This gene (HbCBF1) was found to respond to cold stress but not drought or ABA stress. The corresponding HbCBF1 protein showed CRT/DRE binding activity in gel shift experiment. To further characterize its molecular function, the HbCBF1 gene was overexpressed in Arabidopsis. The HbCBF1 over expression (OE) line showed enhanced cold resistance and relatively slow dehydration, and the expression of Arabidopsis CBF pathway downstream target genes, e.g. AtCOR15a and AtRD29a, were significantly activated under non-acclimation condition. These data suggest HbCBF1 gene is a functional member of the CBF gene family, and may play important regulation function in rubber tree.

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