Guangxi Orthopedic Traumatology Hospital

Nanning, China

Guangxi Orthopedic Traumatology Hospital

Nanning, China
SEARCH FILTERS
Time filter
Source Type

Yang Y.,Guangxi Orthopedic Traumatology Hospital | Li X.-F.,Guangxi Orthopedic Traumatology Hospital | Luo D.-M.,Guangxi University | Wen C.-H.,Guangxi University
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Defatted and deproteinsed cancellous bone allograft has good biological properties. It has a three- dimensional structure that is the same as the recipient's, stable mechanical properties, weak rejection and excellent cytocompatibility. Objective: To prepare defatted and deproteinsed cancellous bone allograft scaffold materials by physiochemical methods, and to analyze physical and chemical characteristics. Methods: Healthy 6-month-old New Zealand white rabbits were chosen, and 20 iliac bones were dissected into 1.0 cm×0.8 cm×0.1 cm bone. Bone scaffold material was prepared by defatting, deproteinization, as well as low and depths freezing temperatures. Its physical and chemical characteristics were tested, and the adhesion rate of the scaffold material and bone marrow stromal stem cells was detected. The scaffold was implanted into the same animal species, and histocompatibility and immunoreaction were observed. Results and Conclusion: The scaffold materials reserved the mesh pore structures of natural bone tissues with the pore ratio of (80.23±5.65)%. Maximum aperture was (318.112±17.51) μm, and minimum aperture was (209.37±11.33) μm. Bone marrow stromal stem cells could not only adhere to the scaffold, but also divide and proliferate on the scaffold. After implantation for 6 weeks, there were no obvious inflammation and rejection around the scaffold interface and a small amount of bone-like tissues formed. These results suggest that the deffatted and deproteinsed cancellous bone scaffold has the appropriate three-dimensional porous structure, and adhesion rate of seed cells is high, besides, it has good biocompatibility and cell-material interface role; at the same time there is a certain osteogenesis function.


Yang Y.,Guangxi Orthopedic Traumatology Hospital | Li X.-F.,Guangxi Orthopedic Traumatology Hospital | Luo D.-M.,Guangxi Traditional Chinese Medical University | Wen C.-H.,Guangxi Traditional Chinese Medical University
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Autologous bone marrow mesenchymal stem cells exhibit multipotential differentiation after in vitro culture. There is increasing concern that whether transplantation of autologous bone marrow mesenchymal stem cells can be used for repair of femoral head necrosis. Objective: To investigate the mechanism by which naringin induces bone marrow mesenchymal stem cells to repair femoral head necrosis in rabbits. Methods: Rabbit bone marrow mesenchymal stem cells were induced with naringin to differentiate into osteoblasts and then loaded onto cancellous bone allograft. Femoral head necrosis rabbit models were prepared. At 3 weeks after femoral head necrosis induction, 12 adult New Zealand rabbits were randomly divided into two groups. In the experimental group, blank cancellous bone allograft was implanted into the left femoral head necrosis region. Identically, nothing was implanted into the left femoral head necrosis region in the control group. The right femoral head necrosis region in each group was implanted with cancellous bone allograft loaded by naringin induced bone marrow mesenchymal stem cells. Results and Conclution: At 8 weeks after implantation, a large amount of callus formed on the surface of the allograft and connected tightly with bone ends, and continuous "bone bridge" formed in some parts. At 12 weeks, relatively mature bone trabeculae were observed in the filling area, which were thicker, and arranged more irregularly compared with normal bone trabeculae. Bone defect region was completely covered by the newly formed bone. These findings suggest that naringin-induced bone marrow mesenchymal stem cells can be used for repair of femoral head necrosis in rabbits.

Loading Guangxi Orthopedic Traumatology Hospital collaborators
Loading Guangxi Orthopedic Traumatology Hospital collaborators