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Zhang T.-T.,Jinan University | Liu Z.-W.,Jinan University | Wang W.-J.,Jinan University | Tong Y.-B.,Jinan University | And 5 more authors.
Heterocycles | Year: 2013

Two new quinoline alkaloids, 14,15-βepoxyscandine (3) and meloscine N-oxide (6), along with six known alkaloids scandine (1), scandine N-oxide (2), meloscandonine (4), meloscine (5), tubotaiwine (7), polyneuridine (8) were isolated from the twigs and leaves of Melodinus suaveolens. The structures of the new compounds were elucidated on the basis of spectroscopic methods and circular dichroism experiments. © The Japan Institute of Heterocyclic Chemistry. Source


Wang G.,Sichuan University | Peng Q.,Sichuan University | Wu L.,Sichuan University | Wang T.,Sichuan University | And 4 more authors.
Journal of Zoology | Year: 2012

The common tokay gecko (Gekko gecko gecko) is widely distributed across southern China, Vietnam and other countries in Southeast Asia. It includes two distinct morphological forms with largely allopatric distributions, which are referred to as the black-spotted tokay and the red-spotted tokay. Considering their different morphological features and distributions, a question has been proposed by taxonomists and still not resolved: do these two forms belong to one subspecies? Previous studies indicated a high genetic variability between them, but did not give a consistent conclusion regarding their taxonomic status. In this work, we employed two mitochondrial genes (cytochrome c oxidase subunit I and cytochrome b) and nine microsatellite DNA loci to explore the phylogenetic relationship and population genetic structure in the two forms from southern China and northern Vietnam. MtDNA results revealed four deeply divergent lineages. Red-spotted tokays were clustered into one lineage, and black-spotted tokays were clustered into three lineages. Microsatellite DNA results confirmed significant levels of genetic differentiation between the red-spotted tokay lineage and one black-spotted tokay lineage, consistent with the mtDNA pattern. In conclusion, considering both morphological and genetic information, we suggest that the red-spotted tokay lineage and one of the black-spotted tokay lineages have probably differentiated into two subspecies. However, more extensive sampling and genetic information are needed to further understand the taxonomic relationships of tokay gecko, particularly the three lineages within the black-spotted tokay. © 2012 The Zoological Society of London. Source


Peng Q.-K.,Sichuan University | Wang G.-C.,Sichuan University | Yang D.,Sichuan University | Yue B.-S.,Sichuan University | And 2 more authors.
Biochemical Systematics and Ecology | Year: 2010

The black-spotted tokay gecko and red-spotted tokay gecko have different distribution areas and are significantly different in appearance but are classified into the same species Gekko gecko. Twelve microsatellite loci were isolated, characterized and evaluated from wild black-spotted tokay geckos for the first time. Of them, nine loci were successfully amplified in red-spotted tokay geckos using multiplex polymerase chain reactions (PCRs). A total of 208 different alleles were observed in the 70 wild black-spotted and red-spotted tokays, and the average number of alleles per locus was 17.3. The average values for observed heterozygosity, expected heterozygosity and polymorphism information content were 0.762, 0.891 and 0.871, respectively, which showed that the wild G. gecko population had a high level of genetic variability. Both black-spotted tokays and red-spotted tokays showed a significant (P < 0.001) deficit of heterozygotes. The red-spotted tokay (HE = 0.881, A = 16.4) had a higher level of genetic variability than black-spotted tokay (HE = 0.804, A = 10.7). The pairwise FST (P < 0.001) estimates of the two types of tokay were 0.143, which indicated that there was a significant level of genetic differentiation between the two. © 2009 Elsevier Ltd. All rights reserved. Source


Peng Q.,Sichuan University | Wang G.,Sichuan University | Tan S.,Sichuan University | Wang T.,Sichuan University | And 2 more authors.
Conservation Genetics Resources | Year: 2011

Thirteen tetranucleotide microsatellite loci were isolated from tetranucleotide microsatellite enriched libraries for the Tokay gecko (gekko gecko). The species is endemic to Southeast Asia, known to be a habitat specialist, and exposed to severe hunting pressures in the mountainous areas of Southeast Asia. These highly polymorphic markers (6-25 alleles) will facilitate the population genetic analyses of this species. In particular, they will enable estimates of gene flow between the two types of the Tokay gecko: the black-spotted Tokay gecko and the redspotted Tokay gecko-a critical element in assessing classification between the two. © Springer Science+Business Media B.V. 2011. Source

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