Guangxi Key Laboratory of Sugarcane Genetic Improvement

Guangxi Key Laboratory of Sugarcane Genetic Improvement

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Lin L.,Guangxi University | Lin L.,Guangxi Academy of Agricultural science | Guo W.,Zhejiang University | Guo W.,National University of Singapore | And 13 more authors.
Applied Microbiology and Biotechnology | Year: 2012

Members of the genus Microbacterium lineage of Gram-positive actinobacteria are increasingly being reported to display significant traits associated with environmental biotechnology and bioengineering. 16SH is a nitrogen-fixing bacterial strain isolated from a surface-sterilized stem of sugarcane grown in Guangxi, China. Analysis of 16S rRNA gene sequences revealed that 16SH belonged to the genus Microbacterium. pPROBE-pTet r plasmids were constructed by cloning the promoter region of the Tet r gene into the promoterless pPROBE-AT, -OT, and -TT vectors derived from the pBBR1 plasmid that has a broad host range of Gram-negative bacteria and sequence similarities to plasmids from Gram-positive bacteria. The pPROBE-pTet r plasmids expressed the gfp reporter gene and were stably maintained in 16SH cells without antibiotic selection in free-living state and in planta. Confocal microscopy on intact roots of micropropagated sugarcane plantlets showed that gfp-tagged 16SH cells formed biofilms on root maturation and elongation zones but not on root meristem zones and root caps, and colonized in intercellular spaces of root cortices. Inoculation of 16SH significantly increased biomass and nitrogen content of micropropagated sugarcane seedlings grown with a nitrogen fertilization of 6.3 mg N/kg soil. 15∈N isotope dilution assays demonstrated that biological nitrogen fixation contributed to this plant growth promotion. This study for the first time demonstrated that the pBBR1-based pPROBE plasmids provided an efficient genetic transfer system for a Gram-positive Microbacterium strain, and that a nitrogen-fixing Microbacterium endophyte colonized in intact host plants and fixed N 2 associated with the host plants. © 2011 Springer-Verlag.

Zhang X.,Zhejiang University | Lin L.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Chen M.,Zhejiang University | Zhu Z.,Zhejiang University | And 5 more authors.
Journal of Hazardous Materials | Year: 2012

Low biomass and shallow root systems limit the application of heavy metal phytoextraction by hyperaccumulators. Plant growth-promoting microbes may enhance hyperaccumulators'phytoextraction. A heavy metal-resistant fungus belonged to the Fusarium oxysporum complex was isolated from the Zn/Cd co-hyperaccumulator Sedum alfredii Hance grown in a Pb/Zn mined area. This Fusarium fungus was not pathogenic to plants but promoted host growth. Hydroponic experiments showed that 500μM Zn2+ or 50μM Cd2+ combined with the fungus increased root length, branches, and surface areas, enhanced nutrient uptake and chlorophyll synthesis, leading to more vigorous hyperaccumulators with greater root systems. Soil experiments showed that the fungus increased root and shoot biomass and S. alfredii-mediated heavy metal availabilities, uptake, translocation or concentrations, and thus increased phytoextraction of Zn (144% and 44%), Cd (139% and 55%), Pb (84% and 85%) and Cu (63% and 77%) from the original Pb/Zn mined soil and a multi-metal contaminated paddy soil. Together, the nonpathogenic Fusarium fungus was able to increase S. alfredii root systems and function, metal availability and accumulation, plant biomass, and thus phytoextraction efficiency. This study showed a great application potential for culturable indigenous fungi other than symbiotic mycorrhizas to enhance the phytoextraction by hyperaccumulators. © 2012 Elsevier B.V.

Chen Z.-L.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Gui Y.-Y.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Qin C.-X.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Wang M.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | And 3 more authors.
Sugar Tech | Year: 2015

Sucrose synthase is one of the key enzymes involved in sucrose metabolism. In order to increase the sucrose content at the molecular level, it is very necessary to understand the biological function of sucrose synthase gene in sugarcane. In this study, homology cloning and rapid amplification of cDNA ends technology were used to obtain the 2898 bp full-length cDNA of ScSuSy4 in sugarcane, which contains an opening reading frame of 2430 bp (GenBank: KM598653). This gene encodes a protein of 809 amino acids with a theoretical molecular weight of 92.31 kDa and an isoelectric point of 6.30. The amino acid sequence homologies between ScSuSy4 and SbSus4, ZmSus3 and OsSus4 from Group II were 99.5, 98.3, and 97.2 %, respectively. Expression analysis showed that at the elongation stage of low-sugar and medium-sugar sugarcane species, the expression level of ScSuSy4 in immature stems was higher than that in mature leaves, whereas in all three high-sugar species, ScSuSy4 gene expression levels were higher in mature leaves than that in immature stems. At the early stage of sucrose accumulation, expression levels of ScSuSy4 in all species were lower in immature stems than that in mature leaves. At different stages of the same species, regardless in immature stems or in mature leaves, ScSuSy4 gene expression reached the highest level at the elongation stage, dropped to the lowest level at early stage of sucrose accumulation, and increased again at late stage of sucrose accumulation. It is putative that ScSuSy4 gene might be involved in the regulation of sucrose distribution via different functions at various stages. This study provides a foundation for further dissection of the biological function of this gene. © 2015 Society for Sugar Research & Promotion

Zhu B.,Zhejiang University | Chen M.,Zhejiang University | Lin L.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Lin L.,Guangxi University | And 5 more authors.
Journal of Bacteriology | Year: 2012

Enterobacter sp. strain SP1 is an endophytic nitrogen-fixing bacterium isolated from a sugarcane stem and can promote plant growth. The draft genome sequence of strain SP1 presented here will promote comparative genomic studies to determine the genetic background of interactions between endophytic enterobacteria and plants. © 2012, American Society for Microbiology.

Wei C.-Y.,Guangxi University | Lin L.,Guangxi University | Lin L.,Key Laboratory of Sugarcane Biotechnology and Genetic Improvement Guangxi | Lin L.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | And 18 more authors.
Biology and Fertility of Soils | Year: 2014

Klebsiella variicola bacteria are found in association with plants. Little is known about their colonization patterns, roles, and mechanisms during association with the plant hosts. Here, we identified a nitrogen-fixing bacterium, DX120E, which was isolated from surface-sterilized roots of the ROC22 sugarcane cultivar, as K. variicola by phylogenetic analyses of its 16S rRNA gene, RNA polymerase β-subunit gene, and DNA gyrase subunit A gene sequences. gfp-tagged DX120E was found to colonize at the roots and aerial parts of micropropagated sugarcane plantlets by fluorescence microscopy and confocal microscopy. DX120E was able to survive in soils and colonize in root epidermal cells, intercellular spaces in root cortices, and leaf mesophyll and vascular tissues. DX120E preferentially colonized at root maturation and elongation zones and entered roots via cracks at the emergence site of lateral roots and at disrupted epidermis. DX120E may penetrate root epidermal cells with the aid of their cellulose-degrading enzymes. 15N isotope dilution assay demonstrated that DX120E was able to fix N2 in association with ROC22 sugarcane plants under gnotobiotic condition. DX120E was also able to promote GT21 cultivar growth and plant uptake of N, P, and K under greenhouse condition. Together, this study for the first time shows that a K. variicola strain is able to colonize in its sugarcane plant hosts, to fix N2 in association with plants, and to promote plant growth. © 2013 Springer-Verlag Berlin Heidelberg.

Jiang Z.-P.,Guangxi Academy of Agricultural science | Li Y.-R.,Chinese Academy of Agricultural Sciences | Li Y.-R.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Wei G.-P.,Guangxi Academy of Agricultural science | And 5 more authors.
Sugar Tech | Year: 2012

The present experiment was conducted to study the effects of vinasse application on physico-chemical properties of soil. Different treatments, viz., (i) spraying water at 105. 0 t/ha (CK), (ii) conventional fertilization + spraying water at 105.0 t/ha (CF) and (iii) soil application of vinasse at 75.0 t/ha + spraying water at 30.0 t/ha, have been applied in two sugarcane fields in Fusui County, Guangxi, during 2005-2006 and 2006-2007, respectively. The results showed that, after 2-3 years of continuous vinasse application to sugarcane fields, the soil bulk density declined, while the total porosity and capillary porosity increased in the plow layer of soil. The soil water stable aggregate content enhanced, but the soil clay content decreased. Soil K content increased, and soil did not showed the phenomenon of acidification. The vinasse application in sugarcane fields resulted in improved physico-chemical properties of soil, and soil hardening and soil acidification were not detected in the field. The present study not only provides the basis of using vinasse as a liquid fertilizer in agriculture fields but also help in reducing the environmental pollution imposed due to disposal of non-treated sugar industry effluents. © 2012 Society for Sugar Research & Promotion.

Yang L.,Chinese Academy of Agricultural Sciences | Yang L.,Key Laboratory of Sugarcane Biotechnology and Genetic Improvement | Yang L.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Liao F.,Guangxi University | And 5 more authors.
Sugar Tech | Year: 2014

This study was conducted to determine the effect of biochar application on the seedling root properties in sugarcane. A pot experiment was done with a sugarcane cultivar ROC 22 with 10 and 20 per cent of laterite (weight/weight) and without biochar application for 60 days. Results showed that biochar application resulted in a better improvement for sugarcane root properties at seedling stage, and biochar application treatment resulted in greater shoot-to-root ratio. Biochar application raised the soil pH, and significantly increased concentration of available P, K and organic matter present in biochar with 20 % application. These findings lead us to suggest that further investigations are needed to assess the complete effect of biochar application on nutrient uptake and use efficiency across the whole sugarcane growth cycle. © 2014, Society for Sugar Research & Promotion.

Huang X.,Chinese Academy of Agricultural Sciences | Huang X.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Huang X.,Guangxi University | Chen M.-H.,Guangxi University | And 7 more authors.
Sugar Tech | Year: 2014

Sugarcane is the most important sugar crop in China and the world, which originated in tropical and subtropical areas and is a thermophilic crop. Extreme weather occurred frequently in worldwide that caused serious cold or frost damage in recent years, resulting in enormous losses in sugarcane production. Abscisic acid (ABA) regulates much important plant physiological and biochemical processes, and induces tolerance to different stresses including cold or frost damage. This experiment investigated the interrelationship between low temperature induced ABA biosynthesis and endogenous hormone balance using two sugarcane varieties, i.e. the cold tolerant variety GT 28 and cold susceptible variety YL 6. Plants were sprayed with ABA 12 h before cold treatment as opposed to the control group, where no additional substances were added. When the plants in the control group were exposed to cold stress, plant cell membranes were injured, and the GA3 (Gibberellic acid 3) decreased, while the relative electric conductivity, MDA (Malondialdehyde), ABA, the ratio of ABA/GA3, ratio of ABA/IAA (Indole acetic acid), and the ratio of ABA/ZR (Zeatin Riboside) all increased under the cold stress, and there are genotypic differences in response to the contents of proline, ABA and GA, and the ratio of ABA/GA exists between the sugarcane variety GT 28, cold tolerant and variety YL 6, cold susceptible under cold stress. The contents of proline and ABA and the ratio of ABA/GA are higher and the content of GA is lower in the cold tolerant variety, which is the vital physiological basis that caused two sugarcane varieties with different cold resistance. In the ABA treatment, the cell membrane injury was effectively alleviated and the contents of MDA and GA3 decreased, but the contents of proline, ABA, and the ratio of ABA/GA3 increased. The decreasing contents of MDA and GA3, in contrast with the increasing contents of proline, ABA, and ratio of ABA/GA3 in sugarcane leaves from the ABA treatment groups, were important factors that can effectively increase cold stress tolerance in sugarcane plants. © 2014, Society for Sugar Research & Promotion.

Liao Q.,Guangxi Academy of Agricultural science | Wei G.-P.,Guangxi Academy of Agricultural science | Chen G.-F.,Guangxi Academy of Agricultural science | Liu B.,Guangxi Academy of Agricultural science | And 8 more authors.
Sugar Tech | Year: 2014

Sugarcane trash addition to the soils was found to alter the structure of microbial communities in soil and increased the number of microbial colonies. The total number of bacterial, fungal and actinomycetes colonies was found to be increased by 2.38, 1.80 and 2.74 folds, respectively, in trash added soil as compared to the conventional one. Further, the addition of trash in soils increased its organic matter and available nutrients, improve the moisture and enhanced the pH, which led to improve physico-chemical properties of soil. Moreover, the trash addition promoted the growth of sugarcane plants, leading to 8.41 and 1.53 t per hectare increase in cane and sucrose yield, respectively. Therefore, the addition of trash to the sugarcane fields improved physico-chemical properties of soil by increasing moisture, organic matter and nutrient content and promoting microbial growth and activities which led to a significant increase in sugarcane production and economic benefits. © 2013, Society for Sugar Research & Promotion.

Ye J.,Fujian Agriculture and forestry University | Que Y.-X.,Fujian Agriculture and forestry University | Li Y.-R.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Xu L.-P.,Fujian Agriculture and forestry University
Sugar Tech | Year: 2016

Nonparametric methods of data envelopment analysis and the Malmquist Index (MI), both based on a time-series analysis, were applied to examine the production efficiency performance and trend of China’s sugar industry from 2004 to 2013 in the four main sugarcane-growing regions of Guangxi, Yunnan, Guangdong and Hainan provinces, which produce more than 90 % of the total sugarcane production in China. The data used in the analysis were from ‘All China Data Compilation of Costs and Returns of Main Agricultural Products (2005–2014)’ of the Price Department of the National Development and Reform Commission. Our analysis revealed that the comprehensive efficiency of sugarcane production in China was relatively low. The average scores of technical efficiency, pure technical efficiency and scale efficiency were 0.760, 0.954 and 0.792, respectively. These scores decreased yearly from 1.000, 1.000 and 1.000 in 2004 to 0.507, 0.978 and 0.518 in 2013, respectively, resulting in a negative impact on the development of the Chinese sugar industry. Additionally, the analysis based on the MI also demonstrated a decline in the trend of sugarcane productivity during 2004–2013 with the total factor productivity index score of 0.894, a slight increase in the technical generalization level with the technical efficiency change index score of 1.002 and a decrease in technological innovation with the technical change index score of 0.880. Our study clearly indicates that Chinese sugarcane productivity needs significant improvement. © 2016 Society for Sugar Research & Promotion

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