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Zhang X.,Zhejiang University | Lin L.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Chen M.,Zhejiang University | Zhu Z.,Zhejiang University | And 5 more authors.
Journal of Hazardous Materials | Year: 2012

Low biomass and shallow root systems limit the application of heavy metal phytoextraction by hyperaccumulators. Plant growth-promoting microbes may enhance hyperaccumulators'phytoextraction. A heavy metal-resistant fungus belonged to the Fusarium oxysporum complex was isolated from the Zn/Cd co-hyperaccumulator Sedum alfredii Hance grown in a Pb/Zn mined area. This Fusarium fungus was not pathogenic to plants but promoted host growth. Hydroponic experiments showed that 500μM Zn2+ or 50μM Cd2+ combined with the fungus increased root length, branches, and surface areas, enhanced nutrient uptake and chlorophyll synthesis, leading to more vigorous hyperaccumulators with greater root systems. Soil experiments showed that the fungus increased root and shoot biomass and S. alfredii-mediated heavy metal availabilities, uptake, translocation or concentrations, and thus increased phytoextraction of Zn (144% and 44%), Cd (139% and 55%), Pb (84% and 85%) and Cu (63% and 77%) from the original Pb/Zn mined soil and a multi-metal contaminated paddy soil. Together, the nonpathogenic Fusarium fungus was able to increase S. alfredii root systems and function, metal availability and accumulation, plant biomass, and thus phytoextraction efficiency. This study showed a great application potential for culturable indigenous fungi other than symbiotic mycorrhizas to enhance the phytoextraction by hyperaccumulators. © 2012 Elsevier B.V.

Yang L.,Chinese Academy of Agricultural Sciences | Yang L.,Key Laboratory of Sugarcane Biotechnology and Genetic Improvement | Yang L.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Liao F.,Guangxi University | And 5 more authors.
Sugar Tech | Year: 2014

This study was conducted to determine the effect of biochar application on the seedling root properties in sugarcane. A pot experiment was done with a sugarcane cultivar ROC 22 with 10 and 20 per cent of laterite (weight/weight) and without biochar application for 60 days. Results showed that biochar application resulted in a better improvement for sugarcane root properties at seedling stage, and biochar application treatment resulted in greater shoot-to-root ratio. Biochar application raised the soil pH, and significantly increased concentration of available P, K and organic matter present in biochar with 20 % application. These findings lead us to suggest that further investigations are needed to assess the complete effect of biochar application on nutrient uptake and use efficiency across the whole sugarcane growth cycle. © 2014, Society for Sugar Research & Promotion.

Jiang Z.-P.,Guangxi Academy of Agricultural science | Li Y.-R.,Chinese Academy of Agricultural Sciences | Li Y.-R.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Wei G.-P.,Guangxi Academy of Agricultural science | And 5 more authors.
Sugar Tech | Year: 2012

The present experiment was conducted to study the effects of vinasse application on physico-chemical properties of soil. Different treatments, viz., (i) spraying water at 105. 0 t/ha (CK), (ii) conventional fertilization + spraying water at 105.0 t/ha (CF) and (iii) soil application of vinasse at 75.0 t/ha + spraying water at 30.0 t/ha, have been applied in two sugarcane fields in Fusui County, Guangxi, during 2005-2006 and 2006-2007, respectively. The results showed that, after 2-3 years of continuous vinasse application to sugarcane fields, the soil bulk density declined, while the total porosity and capillary porosity increased in the plow layer of soil. The soil water stable aggregate content enhanced, but the soil clay content decreased. Soil K content increased, and soil did not showed the phenomenon of acidification. The vinasse application in sugarcane fields resulted in improved physico-chemical properties of soil, and soil hardening and soil acidification were not detected in the field. The present study not only provides the basis of using vinasse as a liquid fertilizer in agriculture fields but also help in reducing the environmental pollution imposed due to disposal of non-treated sugar industry effluents. © 2012 Society for Sugar Research & Promotion.

Wei C.-Y.,Guangxi University | Lin L.,Guangxi University | Lin L.,Key Laboratory of Sugarcane Biotechnology and Genetic Improvement Guangxi | Lin L.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | And 18 more authors.
Biology and Fertility of Soils | Year: 2014

Klebsiella variicola bacteria are found in association with plants. Little is known about their colonization patterns, roles, and mechanisms during association with the plant hosts. Here, we identified a nitrogen-fixing bacterium, DX120E, which was isolated from surface-sterilized roots of the ROC22 sugarcane cultivar, as K. variicola by phylogenetic analyses of its 16S rRNA gene, RNA polymerase β-subunit gene, and DNA gyrase subunit A gene sequences. gfp-tagged DX120E was found to colonize at the roots and aerial parts of micropropagated sugarcane plantlets by fluorescence microscopy and confocal microscopy. DX120E was able to survive in soils and colonize in root epidermal cells, intercellular spaces in root cortices, and leaf mesophyll and vascular tissues. DX120E preferentially colonized at root maturation and elongation zones and entered roots via cracks at the emergence site of lateral roots and at disrupted epidermis. DX120E may penetrate root epidermal cells with the aid of their cellulose-degrading enzymes. 15N isotope dilution assay demonstrated that DX120E was able to fix N2 in association with ROC22 sugarcane plants under gnotobiotic condition. DX120E was also able to promote GT21 cultivar growth and plant uptake of N, P, and K under greenhouse condition. Together, this study for the first time shows that a K. variicola strain is able to colonize in its sugarcane plant hosts, to fix N2 in association with plants, and to promote plant growth. © 2013 Springer-Verlag Berlin Heidelberg.

Zhu B.,Zhejiang University | Chen M.,Zhejiang University | Lin L.,Guangxi Key Laboratory of Sugarcane Genetic Improvement | Lin L.,Guangxi University | And 5 more authors.
Journal of Bacteriology | Year: 2012

Enterobacter sp. strain SP1 is an endophytic nitrogen-fixing bacterium isolated from a sugarcane stem and can promote plant growth. The draft genome sequence of strain SP1 presented here will promote comparative genomic studies to determine the genetic background of interactions between endophytic enterobacteria and plants. © 2012, American Society for Microbiology.

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