Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement

Nanning, China

Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement

Nanning, China
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Miao J.,Tianjin University | Wei K.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Li X.,Tianjin University | Zhao C.,Tianjin University | And 4 more authors.
Journal of Food Science and Technology | Year: 2017

Chemical composition and antioxidant activity of fresh and boiled Chaenomeles speciosa (CS) slices dried by different drying methods were determined. Data were analyzed by principle component analysis and cluster analysis. All dried boiled CS from dried fresh CS slices form main cluster. The results also demonstrated that both drying methods, freeze drying and hot air drying at 60 °C had good potential in the industrial drying of fresh and boiled CS. Fresh CS dried by hot air drying at 60 °C was more suitable for the industrial production. © 2017 Association of Food Scientists & Technologists (India)


Zhang L.,Inner Mongolia Medical College | Zhang L.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Wei K.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Xu J.,Inner Mongolia Medical College | And 6 more authors.
Journal of Ethnopharmacology | Year: 2016

Ethnopharmacological relevance Belamcanda chinensis (L.) DC is the sole species in the genus Belamcanda Adans. (Iridaceae), found mainly in Northeast Asia. Bombus chinensis has long been used in traditional Chinese medicine for its multiple therapeutic uses in the form of antipyretic agents, antidote, expectorant, antiphlogistic and analgesic. Aim of the review This manuscript comprehensively summarizes the various studies published in recent years on the botany, ethnopharmacology, phytochemistry, biological activity and toxicology of B. chinensis. We hope to provide a foundation for future studies on the mechanism of action and development of better therapeutic agents based on B. chinensis. Material and methods All information available on B. chinensis was collected using electronic search engines, such as PubMed, SciFinder Scholar, CNKI, TPL (www.theplantlist.org), Google Scholar and Web of Science. Results The analysis shown that ethno-medical uses of B. chinensis have been recorded in China, Japan and Korea since a long time. Based on a phytochemical investigation, this plant contains flavonoids, terpenoids, quinones, phenolic compounds, ketones, organic acids, etc. Crude extracts and pure compounds isolated from B. chinensis exhibited various biological effects. Conclusions In light of its long traditional use and the modern phytochemical and pharmacological studies summarized here, B. chinensis is known to be a promising medicinal plant with the isolated extracts and chemical components showing a wide range of biological activities. Thus, it is imperative that the necessary programs and value assessment of B. chinensis be established for further studies. It is also important that the synergistic or antagonistic effects of this traditional herbal medicine are investigated in depth to identify more bioactive components by bioactivity-guided isolation strategies, and to illustrate the mechanisms of action targeting on ethnomedical uses. Future clinical studies can also focus on the main therapeutic aspects, toxicity and adverse effects of B. chinensis. © 2016 Elsevier Ireland Ltd. All rights reserved.


Cai J.,Guangxi University of Science and Technology | Cai J.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Zhang C.,Guangxi University of Science and Technology | Wei K.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement
Agro Food Industry Hi-Tech | Year: 2017

Metabolic engineering of natural medicines refers to studying natural medicine extraction process and secondary metabolite components so as to increase the content of certain critical secondary metabolite or its precursor. Increased content of some critical secondary metabolites is of vital significance to economic and social development. However, natural medicines have complex chemical components and contain diverse effective components. With accelerate modernization of TCM and its increasing position in the international market, there has been a higher requirement of extracting effective components from natural medicines. These paper first analyzed technical paths of natural medicine extraction, specific extraction methods and methods to optimize the extraction process. Next, the case of piroformospora indica secondary metabolite extraction process was adopted to verify effectiveness of the response surface method. It was hoped that this research could contribute to improvement of natural medicine extraction techniques and methods and solution of the issue of medicinal sources confronting China.


Qin L.,Guangxi Medical College | Cheng Y.,Guangxi Medical College | Mo D.-D.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Li R.-L.,Guangxi Medical College | And 2 more authors.
Chinese Pharmaceutical Journal | Year: 2017

OBJECTIVE: To research the effect and mechanism of ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep. METHODS: The 70 SD male rats were randomly divided into 7 groups: normal control group, model group, tripterginum glycosides(TG) group, dexamethasone(DXM) group, ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep. low, medium and high dosage groups(0.063, 0.126, 0.252 g·kg-1·d-1), the CIA(collagen induced arthritis) model of rats was adopted. Under 28 days intragastric administration, the rats' state, weight, degree of paw swelling, arthritis index and pathological changes of ankle joints were observed. Their serologic contents of interleukin 1β(IL-1β), interleukin-6(IL-6), and tumor necrosis factor-α(TNF-α) were examined by means of ELISA, and the expression of NF-κB p65 mRNA in joint synovial tissues were tested via quantitative Real-time PCR method. RESULTS: The ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep. is found obviously effective in inhibiting CIA rats' paw swelling, decreasing serologic content of IL-1β, IL-6, and TNF-α, as well as the expression of NF-κB p65 mRNA in synovial tissues.Its effect is dosage-related, and stronger than that of TG, weaker than that of DXM. CONCLUSION: Kadsura longipedunculata Finet et Gagnep. is, to some extent, effective against RA, the mechanism of which is related to the transcription of NF-κB p65. Copyright 2017 by the Chinese Pharmaceutical Association.


Li L.-X.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Wu Q.-H.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Cai J.-Y.,Guangxi University of Science and Technology | Lin W.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Wei K.-H.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement
Chinese Traditional and Herbal Drugs | Year: 2014

Objective: To solve the shortage problem of large-scale planting the seedlings in the roots of Ficus hirta by rapid propagation in vitro tissue culture. Methods: The MS and 1/2 MS media were used as basic media, The multi factor combination of plant growth regulator (6-BA, NAA, 2, 4-D, IAA, KT, and IBA) on the seedling subculture and root culture was studied by orthogonal design. Results: The best medium for the adventitious bud induction was MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L, 72 adventitious buds were obtained by differentiation with 20 d induction of explants; The best medium for cluster inducing and subculture was MS + 1.0 mg/L 6-BA + 0.3 mg/L IAA + 0.3 mg/L KT; The best rooting medium was 1/2 MS + 1.0 mg/L IBA + 0.3 mg/L NAA, and the rooting rate was 100%. Being suitably Transplanted to peat-perlite (1:1) matrix, the 30 d survival rate was 93%. Conclusion: The tissue culture for the roots of F. hirta could be used to produce test-tube seedlings for large scale planting. ©, 2014, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.


Min D.-D.,Guangxi University | Tang M.-Q.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Li G.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Qu X.-S.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Miao J.-H.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement
Zhongguo Zhongyao Zazhi | Year: 2015

According to the transcriptome dataset of Panax notoginseng, the key geranylgeranyl pyrophosphate synthase gene (GGPPS) in terpenoid backbone biosynthesis was selected to be cloned. Using specific primer pairs combining with RACE (rapid amplification of cDNA ends) technique, the full-length cDNA sequence with 1 203 bp, which containing a 1 035 bp open reading frame, was cloned and named as PnGGPPS. The corresponding full-length DNA sequence contained 2 370 bp, consisted of 1 intron and 2 exons. The deduced protein PnGGPPS contained 344 amino acids and shared more than 73% identity with GGPPS from Ricinus communis and Salvia miltiorrhiza. PnGGPPS also had specific Aspartic acid enrichment regions and other conserved domains, which belonged to the Isoprenoid-Biosyn-Cl superfamily. The quantitative real-time PCR showed that PnGGPPS expressed in different tissues of 1, 2, 3 years old root, stem, leaf and 3 years old flower, and the expression level in 3 years old leaf was significant higher than that in other organs, which suggested that it might not only be involved in the regulation of the growth and development, but also be associated with the biosynthesis of chlorophyll and carotenoids, the development of chloroplast, the shade habit and the quality formation of P. notoginseng.


Tang M.-Q.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Min D.-D.,Guangxi University | Li G.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Jiang N.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Ye Y.-F.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement
Yaoxue Xuebao | Year: 2015

With homology cloning approaches coupling with RACE (rapid-amplification of cDNA ends) techniques, the full-length coding sequence of pathogenesis-related protein PR10-1 with differential expression was cloned from the total RNA of the root of Panax notoginseng, and its function was explored furtherly. As a result, the longest 465 bp ORF (named as PnPR10-l with the Accession No. KJ741402 in GenBank) was detected from the cloned sequence with full-length of cDNA of 863 bp. The corresponding peptide encoded consisted of 155 amino acids, contained some domains such as Bet-v-I, and showed high similarity with that from Panax ginseng by analysis of phylogenetic trees created from the alignments. Real-time quantitative PCR showed that the expression of PnPR10-l gene was constitutive in different tissues of 1-3 year old plant, suggesting that it might be involved in growth, development, and secondary metabolism; yet it was up-regulated significantly with the infection of Fusarium oxysporum in root, suggesting that it might be involved in defense against many diseases including root rot in P. notoginseng.


PubMed | Inner Mongolia Medical College, Guangxi key Laboratory of Medicinal Resources Protection and Genetic Improvement and Texas A&M University
Type: | Journal: Journal of ethnopharmacology | Year: 2016

Belamcanda chinensis (L.) DC is the sole species in the genus Belamcanda Adans. (Iridaceae), found mainly in Northeast Asia. Bombus chinensis has long been used in traditional Chinese medicine for its multiple therapeutic uses in the form of antipyretic agents, antidote, expectorant, antiphlogistic and analgesic.This manuscript comprehensively summarizes the various studies published in recent years on the botany, ethnopharmacology, phytochemistry, biological activity and toxicology of B. chinensis. We hope to provide a foundation for future studies on the mechanism of action and development of better therapeutic agents based on B. chinensis.All information available on B. chinensis was collected using electronic search engines, such as PubMed, SciFinder Scholar, CNKI, TPL (www.theplantlist.org), Google Scholar and Web of Science.The analysis shown that ethno-medical uses of B. chinensis have been recorded in China, Japan and Korea since a long time. Based on a phytochemical investigation, this plant contains flavonoids, terpenoids, quinones, phenolic compounds, ketones, organic acids, etc. Crude extracts and pure compounds isolated from B. chinensis exhibited various biological effects.In light of its long traditional use and the modern phytochemical and pharmacological studies summarized here, B. chinensis is known to be a promising medicinal plant with the isolated extracts and chemical components showing a wide range of biological activities. Thus, it is imperative that the necessary programs and value assessment of B. chinensis be established for further studies. It is also important that the synergistic or antagonistic effects of this traditional herbal medicine are investigated in depth to identify more bioactive components by bioactivity-guided isolation strategies, and to illustrate the mechanisms of action targeting on ethnomedical uses. Future clinical studies can also focus on the main therapeutic aspects, toxicity and adverse effects of B. chinensis.


Wang S.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Zhou X.-L.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Gong X.-M.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Fan X.-Y.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Lan M.-S.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement
Journal of Asian Natural Products Research | Year: 2015

Two new norditerpenoid alkaloids with lycoctonine skeleton, anthriscifolcones A (1) and B (2), were isolated from the whole plant of Delphinium anthriscifolium var. Majus by extensive column chromatography. Their structures were established by IR, MS, 1H NMR, 13C NMR, and 2D NMR methods (including HSQC, 1H–1H COSY, HMBC, and NOESY experiments). © 2015 Taylor & Francis


PubMed | Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement
Type: Journal Article | Journal: Journal of Asian natural products research | Year: 2016

Two new norditerpenoid alkaloids with lycoctonine skeleton, anthriscifolcones A (1) and B (2), were isolated from the whole plant of Delphinium anthriscifolium var. Majus by extensive column chromatography. Their structures were established by IR, MS, (1)H NMR, (13)C NMR, and 2D NMR methods (including HSQC, (1)H-(1)H COSY, HMBC, and NOESY experiments).

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