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Tang M.-Q.,Guangxi Botanical Garden of Medicinal Plant | Tang M.-Q.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Wei R.-C.,Guangxi Botanical Garden of Medicinal Plant | Wei R.-C.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | And 7 more authors.
Chinese Traditional and Herbal Drugs | Year: 2012

Objective: To evaluate the genetic diversity of germphlasm resources for Sarcandra glabra and provide biological reference for germplasm resources protection, genetic relationship analysis, breeding, and introduction and cultivation of S. glabra and other Chinese herbal medicines. Methods: Amplified fragment length polymorphism (AFLP) marker was developed to analyze the genetic diversity among 18 samples of S. glabra from various habitats in Guangxi region, and cluster analysis and principal coordinate analysis were revealed by the NTSYS software. Results: Eight out of sixty-four AFLP primer combinations selected were used for amplification and a total of 319 unambiguous bands were obtained, among which 251 (PPB = 78.68%) were polymorphic. The results of cluster analysis and principal coordinate analysis showed that there was a certain genetic basis of diversity in S. glabra. The genetic similarity coefficients (0.6303-0.8366) were bigger while the genetic distance was closer. Conclusion: The genetic diversity of germplasm resources for S. glabra in Guangxi region is not abundant and protection strategies should be carried out on S. glabra. Source


Min D.-D.,Guangxi University | Tang M.-Q.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Li G.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Qu X.-S.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Miao J.-H.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement
Zhongguo Zhongyao Zazhi | Year: 2015

According to the transcriptome dataset of Panax notoginseng, the key geranylgeranyl pyrophosphate synthase gene (GGPPS) in terpenoid backbone biosynthesis was selected to be cloned. Using specific primer pairs combining with RACE (rapid amplification of cDNA ends) technique, the full-length cDNA sequence with 1 203 bp, which containing a 1 035 bp open reading frame, was cloned and named as PnGGPPS. The corresponding full-length DNA sequence contained 2 370 bp, consisted of 1 intron and 2 exons. The deduced protein PnGGPPS contained 344 amino acids and shared more than 73% identity with GGPPS from Ricinus communis and Salvia miltiorrhiza. PnGGPPS also had specific Aspartic acid enrichment regions and other conserved domains, which belonged to the Isoprenoid-Biosyn-Cl superfamily. The quantitative real-time PCR showed that PnGGPPS expressed in different tissues of 1, 2, 3 years old root, stem, leaf and 3 years old flower, and the expression level in 3 years old leaf was significant higher than that in other organs, which suggested that it might not only be involved in the regulation of the growth and development, but also be associated with the biosynthesis of chlorophyll and carotenoids, the development of chloroplast, the shade habit and the quality formation of P. notoginseng. Source


Tang M.-Q.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Min D.-D.,Guangxi University | Li G.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Jiang N.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Ye Y.-F.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement
Yaoxue Xuebao | Year: 2015

With homology cloning approaches coupling with RACE (rapid-amplification of cDNA ends) techniques, the full-length coding sequence of pathogenesis-related protein PR10-1 with differential expression was cloned from the total RNA of the root of Panax notoginseng, and its function was explored furtherly. As a result, the longest 465 bp ORF (named as PnPR10-l with the Accession No. KJ741402 in GenBank) was detected from the cloned sequence with full-length of cDNA of 863 bp. The corresponding peptide encoded consisted of 155 amino acids, contained some domains such as Bet-v-I, and showed high similarity with that from Panax ginseng by analysis of phylogenetic trees created from the alignments. Real-time quantitative PCR showed that the expression of PnPR10-l gene was constitutive in different tissues of 1-3 year old plant, suggesting that it might be involved in growth, development, and secondary metabolism; yet it was up-regulated significantly with the infection of Fusarium oxysporum in root, suggesting that it might be involved in defense against many diseases including root rot in P. notoginseng. Source


Li L.-X.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Wu Q.-H.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Cai J.-Y.,Guangxi University of Science and Technology | Lin W.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Wei K.-H.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement
Chinese Traditional and Herbal Drugs | Year: 2014

Objective: To solve the shortage problem of large-scale planting the seedlings in the roots of Ficus hirta by rapid propagation in vitro tissue culture. Methods: The MS and 1/2 MS media were used as basic media, The multi factor combination of plant growth regulator (6-BA, NAA, 2, 4-D, IAA, KT, and IBA) on the seedling subculture and root culture was studied by orthogonal design. Results: The best medium for the adventitious bud induction was MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L, 72 adventitious buds were obtained by differentiation with 20 d induction of explants; The best medium for cluster inducing and subculture was MS + 1.0 mg/L 6-BA + 0.3 mg/L IAA + 0.3 mg/L KT; The best rooting medium was 1/2 MS + 1.0 mg/L IBA + 0.3 mg/L NAA, and the rooting rate was 100%. Being suitably Transplanted to peat-perlite (1:1) matrix, the 30 d survival rate was 93%. Conclusion: The tissue culture for the roots of F. hirta could be used to produce test-tube seedlings for large scale planting. ©, 2014, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved. Source


Liu F.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Wang S.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | Cai J.-Y.,Guangxi University of Science and Technology | Gong X.-M.,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement | And 2 more authors.
Chinese Journal of New Drugs | Year: 2015

Objective: To establish the quality standard of Compound Tiancha Jiangzhi Mixture. Methods: Rubus Suavissmus and Flos Chrysanthemi in Compound Tiancha Jiangzhi Mixture were identified by a series of TLC methods. The content of rubusoside was determined by HPLC using a Diamonsil C18(250 mm×4.6 mm, 5 μm) column. Gradient elution was conducted with the mobile phase A of methanol and B of water at flow rate of 0.8 mL·min-1. The detection wavelength was 208 nm, and the column temperature was maintained at 25℃. Results: The TLC method was successfully established for the identification of Rubus Suavissmus and Flos Chrysanthemi. The principal spots in the TLC chromatogram of the test solution were similar in position, colour and size to those of the reference solution. The linear range of the calibration curve of rubusoside was 1.005~6.030 μg, and the average recovery was 96.29%(RSD=1.73%, n=9). The average content of rubusoside was 0.008 0 mg·mL-1. Conclusion: The established method is repeatable and rebliable, and can be used for the quality control of Compound Tiancha Jiangzhi Mixture. © 2015, Chinese Journal of New Drugs Co. Ltd. All right reserved. Source

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