Guangxi Institute of Fisheries

Nanning, China

Guangxi Institute of Fisheries

Nanning, China
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Wu Y.-R.,CAS Institute of Botany | Gong Q.-F.,CAS Institute of Botany | Fang H.,CAS Institute of Botany | Liang W.-W.,Guangxi Institute of Fisheries | And 2 more authors.
Fish and Shellfish Immunology | Year: 2013

The paper describes the effect of a diet supplemented with the Chinese traditional herbal medicine Sophora flavescens on the immunity and disease resistance of an Oreochromis niloticus GIFT strain. Experimental diets containing 0.025%, 0.050%, 0.100%, 0.200%, and 0.400% S. flavescens, as well as a control group without S. flavescens were used. We tested the non-specific humoral immune responses (lysozyme, antiprotease, and complement) and cellular immune responses (reactive oxygen species and nitrogen species production and myeloperoxidase), as well as disease resistance against Streptococcus agalactiae. S. flavescens supplementation at all dose significantly enhanced serum lysozyme, antiprotease, and natural hemolytic complement activity. Similarly, all S. flavescens doses enhanced cellular myeloperoxidase activity. The increased production of reactive oxygen species and reactive nitrogen intermediates by peripheral blood leucocytes was observed in most of the treatment groups throughout the test period. The fish fed 0.100% S. flavescens had a percent mortality of 21.1% and a relative percent survival of 73.3% compared with the group fed the basal diet during the S. agalactiae challenge. The results suggest that S. flavescens can be recommended as a tilapia feed supplement to enhance fish immunity and disease resistance against S. agalactiae. © 2012 Elsevier Ltd.

Zhang E.,CAS Wuhan Institute of Hydrobiology | Zhang M.,Nanjing Institute of Environmental Sciences | Han Y.-Q.,Guangxi Institute of Fisheries
Zootaxa | Year: 2011

A new garrain genus and species are described from a tributary of the Zuo-River of the Pearl River drainage in Guangxi Province, South China. Cophecheilus, new genus, is separated from all other Asian garrains by its uniquely modified oromandibular morphology; i.e., rostral cap with a shallow, arched, subdistal depression extending almost the full length of its ventral margin; upper lip greatly reduced to a thin membranous fold medially adnate to the upper jaw, but reflected slightly from its distal margin, and laterally produced into a frenum connecting the upper jaw with the lower lip. The new species, Cophecheilus bamen, has an inconspicuous longitudinal black stripe extending along each side of the body. Copyright © 2011 Magnolia Press.

Zeng D.,Guangxi Institute of Fisheries | Chen X.,Guangxi Institute of Fisheries | Xie D.,Guangxi Institute of Fisheries | Zhao Y.,Guangxi Institute of Fisheries | And 7 more authors.
PLoS ONE | Year: 2013

Background: The Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology. Methodology/Principal Findings: We obtained 126919 and 102181 high-quality reads from TSV-infected and non-infected (control) L. vannamei cDNA libraries, respectively. The overall de novo assembly of cDNA sequence data generated 15004 unigenes, with an average length of 507 bp. Based on BLASTX search (E-value <10-5) against NR, Swissprot, GO, COG and KEGG databases, 10425 unigenes (69.50% of all unigenes) were annotated with gene descriptions, gene ontology terms, or metabolic pathways. In addition, we identified 770 microsatellites and designed 497 sets of primers. Comparative genomic analysis revealed that 1311 genes differentially expressed in the infected shrimp compared to the controls, including 559 up- and 752 down- regulated genes. Among the differentially expressed genes, several are involved in various animal immune functions, such as antiviral, antimicrobial, proteases, protease inhibitors, signal transduction, transcriptional control, cell death and cell adhesion. Conclusions/Significance: This study provides valuable information on shrimp gene activities against TSV infection. Results can contribute to the in-depth study of candidate genes in shrimp immunity, and improves our current understanding of this host-virus interaction. In addition, the large amount of transcripts reported in this study provide a rich source for identification of novel genes in shrimp. © 2013 Zeng et al.

Chen X.,Guangxi Institute of Fisheries | Zeng D.,Guangxi Institute of Fisheries | Xie D.,Guangxi Institute of Fisheries | Zhao Y.,Guangxi Institute of Fisheries | And 7 more authors.
PLoS ONE | Year: 2013

Pacific white shrimp (Litopenaeus vannamei) is the most extensively farmed crustacean species in the world. White spot syndrome virus (WSSV) is one of the major pathogens in the cultured shrimp. However, the molecular mechanisms of the host-virus interaction remain largely unknown. In this study, the impact of WSSV infection on host gene expression in the hepatopancreas of L. vannamei was investigated through the use of 454 pyrosequencing-based RNA-Seq of cDNA libraries developed from WSSV-challenged shrimp or normal controls. By comparing the two cDNA libraries, we show that 767 host genes are significantly up-regulated and 729 genes are significantly down-regulated by WSSV infection. KEGG analysis of the differentially expressed genes indicated that the distribution of gene pathways between the up- and down-regulated genes is quite different. Among the differentially expressed genes, several are found to be involved in various processes of animal defense against pathogens such as apoptosis, mitogen-activated protein kinase (MAPK) signaling, toll-like receptor (TLR) signaling, Wnt signaling and antigen processing and presentation pathways. The present study provides valuable information on differential expression of L. vannamei genes following WSSV infection and improves our current understanding of this host-virus interaction. In addition, the large number of transcripts obtained in this study provides a strong basis for future genomic research on shrimp. © 2013 Chen et al.

Zeng D.G.,Guangxi Institute of Fisheries | Lei A.Y.,Guangxi Institute of Fisheries | Chen X.H.,Guangxi Institute of Fisheries
Genetics and Molecular Research | Year: 2013

The macrophage migration inhibitory factor (MIF) is an important proinflammatory cytokine that mediates both innate and adaptive immune responses. In this study, we identified a homolog of MIF in the Pacific white shrimp Litopenaeus vannamei. The MIF cDNA contained a 363-bp open reading frame encoding a 120-amino acid protein with a calculated molecular mass of 13.442 kDa and a theoretical isoelectric point of 6.57. The L. vannamei MIF shared high amino acid identity with MIFs of other invertebrates. Tissue distribution analysis by quantitative real-time polymerase chain reaction (qRT-PCR) revealed that the L. vannamei MIF was abundantly expressed in the blood, heart, and hepatopancreas, was moderately expressed in the gill, and was weakly expressed in the muscle and intestine. Furthermore, in order to gain a basic understanding of the role of MIF in the shrimp immune response against viral infection, its mRNA expression was determined in the hepatopancreas of L. vannamei at different stages after white spot syndrome virus (WSSV) challenge using qRT-PCR. The result indicated that the expression of MIF was significantly upregulated after WSSV injection, suggesting that MIF may be involved in the response to viral infection in shrimp. © FUNPEC-RP.

Li M.,Sun Yat Sen University | Li M.,Guangxi Institute of Fisheries | Li C.,Sun Yat Sen University | Ma C.,Guangxi University | And 7 more authors.
Developmental and Comparative Immunology | Year: 2014

C-type lectins (CTLs) play crucial roles in innate immune responses in invertebrates by recognizing and eliminating microinvaders. In this study, a CTL from pacific white shrimp Litopenaeus vannamei (LvCTL3) was identified. LvCTL3 contains a single C-type lectin-like domain (CTLD), which shows similarities to those of other shrimp CTLs and has a mutated 'EPD' motif in Ca2+-binding site 2. LvCTL3 mRNA can be detected in all tested tissues and expression of LvCTL3 in gills was up-regulated after Lipopolysaccharides, poly (I:C), Vibrio parahaemolyticus and white spot syndrome virus (WSSV) challenges, suggesting activation responses of LvCTL3 to bacterial, virus and immune stimulant challenges. The 5'flanking regulatory region of LvCTL3 was cloned and we identified a NF-κB binding motif in the LvCTL3 promoter region. Dual-luciferase reporter assays indicated that over-expression of L. vannamei dorsal can dramatically up regulate the promoter activity of LvCTL3, suggesting that LvCTL3 expression could be regulated through NF-κB signaling pathway. As far as we know, this is the first report on signaling pathway involve in shrimp CTLs expression. The recombinant LvCTL3 protein was expressed in Escherichia coli and purified by Ni-affinity chromatography. The purified LvCTL3 can agglutinate Gram-negative microbe Vibrio alginolyticus and V. parahaemolyticus and Gram-positive bacteria Bacillus subtilis in the presence of calcium ions, but cannot agglutinate Gram-positive bacteria Streptococcus agalactiae. The agglutination activity of LvCTL3 was abolished when Ca2+ was chelated with EDTA, suggesting the function of LvCTL3 is Ca2+-dependent. In vivo challenge experiments showed that the recombinant LvCTL3 protein can significantly reduce the mortalities of V. parahemolyticus and WSSV infection, indicating LvCTL3 might play significant roles in shrimp innate immunity defense against bacterial and viral infection. © 2014 Elsevier Ltd.

Yang J.,Guangxi Teachers Education University | Wu T.-J.,Guangxi Institute of Fisheries | Yang J.-X.,CAS Kunming Institute of Zoology
Environmental Biology of Fishes | Year: 2012

A new cave-dwelling species, Triplophysa macrocephala sp. nov. is described based on specimens collected from a karst cave in Renguang village, Lihu Town, Nandan County, Guangxi Zhuang Autonomous Region, China. The new species can be distinguished from its congeners by possessing the following combination of characters: pectoral fin not reaching beyond pelvic-fin origin; caudal fin forked; body smooth or scaleless; dorsal-fin origin anterior to pelvic-fin origin, edge of dorsal fin truncate; dorsal fin with eight branched rays; anal fin with five branched rays; eye small and vestigial; lower jaw arched with a median notch; air-bladder wrapped in bony capsule, lateral enlarged, posterior chamber of air-bladder degenerated. © 2011 Springer Science+Business Media B.V.

Gan X.,Guangxi Institute of Fisheries
Dong wu xue yan jiu = Zoological research / "Dong wu xue yan jiu" bian ji wei yuan hui bian ji | Year: 2013

This study describes a new blind barbine fish species, Sinocyclocheilus anshuiensis sp. nov. discovered based on five specimens collected from a cave in Luolou town, Lingyun County, Guangxi, China, in June and July 2012. Sinocyclocheilus anshuiensis is distinguished from other species of Sinocyclocheilus by having the following combination of characteristics: dorsal fin with 7 branched rays, last unbranched dorsal-fin ray weak with serrations on posterior edge of its lower part; pelvic-fin origin anterior to dorsal-fin origin; dorsal profile of head sharply uplift, a forward flesh tuber present on frontal; body covered with scales, and lateral line with 34-38 scales, lateral line scales are as big as their neighbor scales; caudal peduncle with developed fresh crests.

Wang Y.,Shanghai Ocean University | Wang Y.,City University of Hong Kong | Hu M.,Shanghai Ocean University | Hu M.,City University of Hong Kong | And 4 more authors.
Science of the Total Environment | Year: 2014

The combined effects of DO and TiO2 (mixed rutile/anatase phase, 7/3) on immune responses in Perna viridis were examined. Mussels were exposed to six combinations of oxygen levels (hypoxia: 1.5mgO2l-1, normoxia: 6.0mgO2l-1) and TiO2 concentrations (0, 2.5mgl-1 and 10mgl-1) for 216h. Mussels were sampled after 24h, 48h, 120h and 216h, and immune parameters of hemocytes, including mortality, phagocytosis, non-specific esterase, ROS production, lysosomal content and total hemocyte count were investigated using flow cytometric assay. Hemocyte mortality was higher under hypoxia than normoxia, and increased with TiO2 concentrations, but no interaction was found between DO and TiO2. Phagocytosis was reduced under hypoxia and decreased with TiO2 exposure, and the interactive effect between time and TiO2 was observed. The percentage of hemocytes showing non-specific esterase activity was lower under hypoxia, and decreased as TiO2 concentration increased with the significant interactive effect of DO and TiO2. ROS production and lysosomal content were lower under hypoxia and reduced as concentration of TiO2 increased, and interactive effect of DO and TiO2 on ROS was evident. THC was significantly affected by the interactive effect between TiO2 and DO, with higher values under normoxia in the presence of TiO2. The present study demonstrated that immune functions of P. viridis were influenced by both nano-TiO2 and hypoxia with some synergistic effects between the two stressors. This implies that DO has to be considered in the evaluation of the toxicity of nano-materials to bivalves. © 2013 Elsevier B.V.

Chen M.,Guangxi University | Chen M.,Guangxi Institute of Fisheries | Wang R.,Guangxi Institute of Fisheries | Li L.-P.,Guangxi Institute of Fisheries | And 6 more authors.
Vaccine | Year: 2012

The immunogenicity identification of epidemic strain is important for the development and application of vaccine. In this study, 85 Streptococcus agalactiae prevalent strains from the tilapia main cultured areas of China were distributed among 10 distinct PFGE genotypes (A-J). For each genotype, one representative strain (S.aA-S.aJ) was selected to develop an inactivated whole-cell bacterial vaccine (VA-VJ), which then underwent a protective immunity test. VA-VJ showed similar relative percent survival (RPS) to the homologous or heterologous strains with the identical genotype, while the average RPS among VA-VJ protecting against itself genotype strains showed large differences (44.71-98.81%). The RPS of VA-VJ vaccinated fish against infections by the mixture of S.aA-S.aJ at 15 days post vaccination (dpv) was ranged from 13.33% to 60.00%, and VB, VD, VF, and VG showed the highest RPS of 60.00%, 46.67%, 53.33% and 60.00% respectively. VB, VD and VG have their own specific protection scope, VB showed strong protective immunity to infections caused by A-D, F, G and J (53.57-100%), and VG showed strong protective immunity to C-H and J (50.00-100%), whereas VD showed weak protective immunity to all non-self genotype strains (14.81-36.67%). The results of the combined vaccination showed that VG+VB group had wider protection scope and higher RPS value than VG+VD group. Our results demonstrated that the protective immunity of S. agalactiae from tilapia was not only associated with their serotypes, but also related to their PFGE genotypes. It is difficult to acquire a single vaccine candidate strain that can protect against all genotype strains from the same serotype. © 2012 Elsevier Ltd.

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