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Yan K.-J.,Guangxi Institute of Chinese Medicine and Pharmaceutical Science | Xu D.-X.,Beijing Normal University | Song Z.-Q.,CAS South China Botanical Garden | Song Z.-Q.,University of Chinese Academy of Sciences
Systematic Botany | Year: 2016

A new species of Rubiaceae, Mycetia fangii, is described and illustrated from Guangxi, South China. The new species morphologically resembles M. mukerjiana but differs in the calyx bearing one short gland on each sinus, the calyx lobes much shorter than the corolla, and shorter bracts and bracteoles. It is one of the species with cauliflorous inflorescences in Mycetia. Further examination of herbarium specimens shows that M. apoensis, M. yatesii, and M. lanceolata (Ridl.) Ridl. (non Miq.) are conspecific with M. cauliflora, the type species of the genus. Thus the three names are here reduced to synonymy of the type species. A key to the species of the genus with cauliflory is provided at the end of this paper. © 2016 by the American Society of Plant Taxonomists.


Chen C.,Peoples Hospital of Guangxi Autonomous Region | Chen W.,Peoples Hospital of Guangxi Autonomous Region | Nong Z.,Guangxi Institute of Chinese Medicine and Pharmaceutical Science | Ma Y.,Maternal and Child Hospital of Guangxi Zhuang Autonomous Region | And 2 more authors.
Cellular Physiology and Biochemistry | Year: 2016

Background/Aims: In this study, we examined whether the combination of hyperbaric oxygen (HBO) and diltiazem therapy provided a cardioprotective effect on myocardial ischemia-reperfusion injury (MIRI) rat model. Methods: Sixty healthy Sprague-Dawley rats were randomly divided into sham, IR, diltiazem (5 mg/kg), HBO (0.25 MPa, 60 min) and combination therapy (HBO plus diltiazem) groups. MIRI model was established by ligating the left anterior descending for 30 min, followed by 60 min of reperfusion. Results: The results show that HBO and diltiazem preconditioning significantly improves cardiac function and myocardial infarction area, increases nitric oxide, endothelial nitric oxide synthase and ATPase (Na+-K+-ATPase and Ca2+-Mg2+-ATPase) activity and decreases levels of oxygen stress, myocardial enzymes and endothelin-1. Notably, HBO and diltiazem preconditioning significantly increased Bcl-2 protein expression and decreased Bax protein and caspase-3 mRNA expression. Conclusions: These data indicate that combination therapy protected against heart MIRI by reducing oxygen stress damage, correcting energy metabolism, improving endothelial function and inhibiting cell apoptosis. © 2016 The Author(s).


PubMed | Peoples Hospital of Guangxi Autonomous Region and Guangxi Institute of Chinese Medicine and Pharmaceutical Science
Type: | Journal: Neurochemical research | Year: 2017

Our previous study demonstrated that hyperbaric oxygen (HBO) improved cognitive impairments mainly by regulating oxidative stress, inflammatory responses and aging-related gene expression. However, a method for preventing cognitive dysfunction has yet to be developed. In the present study, we explored the protective effects of HBO on the cholinergic system and apoptosis in D-galactose (D-gal)-treated mice. A model of aging was established via systemic intraperitoneal injection of D-gal daily for 8weeks. HBO was administered during the last 2weeks of D-gal injection. Our results showed that HBO in D-gal-treated mice significantly improved behavioral performance on the open field test and passive avoidance task. Studies on the potential mechanisms of this effect showed that HBO significantly reduced oxidative stress and blocked the nuclear factor-B pathway. Moreover, HBO significantly increased the levels of choline acetyltransferase and acetylcholine and decreased the activity of acetylcholinesterase in the hippocampus. Furthermore, HBO markedly increased expression of the anti-apoptosis protein Bcl-2 and glial fibrillary acidic protein meanwhile decreased expression of the pro-apoptosis proteins Bax and caspase-3. Importantly, there was a significant reduction in expression of A-related genes, such as amyloid precursor protein, -site amyloid cleaving enzyme-1 and cathepsin B mRNA. These decreases were accompanied by significant increases in expression of neprilysin and insulin-degrading enzyme mRNA. Moreover, compared with the Vitamin E group, HBO combined with Vitamin E exhibited significant difference in part of the above mention parameters. These findings suggest that HBO may act as a neuroprotective agent in preventing cognitive impairments.


PubMed | Peoples Hospital of Guangxi Autonomous Region and Guangxi Institute of Chinese Medicine and Pharmaceutical Science
Type: Journal Article | Journal: Neurochemical research | Year: 2016

Memory decline is characteristic of aging and age-related neurodegenerative disorders. This study was designed to investigate the protective effect of hyperbaric oxygen (HBO) against cognitive impairment induced by D-galactose (D-gal) in mice. D-gal was intraperitoneally injected into mice daily for 8 weeks to establish the aging model. HBO was simultaneously administered once daily. The results indicate that HBO significantly reversed D-gal-induced learning and memory impairments. Studies on the potential mechanisms of this action showed that HBO significantly reduced oxidative stress by increasing superoxide dismutase, glutathione peroxidase, and catalase levels, as well as the total anti-oxidation capability, while decreasing the content of malondialdehyde, nitric oxide, and nitric oxide synthase in the hippocampal CA1 region. HBO also inhibited advanced glycation end-product formation and decreased levels of tumor necrosis factor- and interleukin-6. Moreover, HBO significantly attenuated D-gal-induced pathological injury in the hippocampus, as well as -amyloid protein


Zhang Z.,Peking Union Medical College | Chu S.-F.,Hunan University | Mou Z.,Peking Union Medical College | Gao Y.,Peking Union Medical College | And 3 more authors.
Molecular and Cellular Neuroscience | Year: 2016

Growing evidence indicates that GQ1b, one of the gangliosides members, contributes to synaptic transmission and synapse formation. Previous studies have shown that GQ1b could enhance depolarization induced neurotransmitter release, while the role of GQ1b in asynchronous release is still largely unknown. Here in our result, we found low concentration of GQ1b, but not GT1b or GD1b (which were generated from GQ1b by plasma membrane-associated sialidases), evoked asynchronous dopamine (DA) release from both clonal rat pheochromocytoma PC12 cells and rat striatal slices significantly. The release peaked at 2min after GQ1b exposure, and lasted for more than 6min. This effect was caused by the enhancement of intracellular Ca2+ and the activation of Pyk2. Inhibition of Pyk2 by PF-431396 (a dual inhibitor of Pyk2 and FAK) or Pyk2 siRNA abolished DA release induced by GQ1b. Moreover, Pyk2 Y402, but not other tyrosine site, was phosphorylated at the peaking time. The mutant of Pyk2 Y402 (Pyk2-Y402F) was built to confirm the essential role of Y402 activation. Further studies revealed that activated Pyk2 stimulated ERK1/2 and p-38, while only the ERK1/2 activation was indispensable for GQ1b induced DA release, which interacted with Synapsin I directly and led to its phosphorylation, then depolymerization of F-actin, thus contributed to DA release. In conclusion, low concentration of GQ1b is able to enhance asynchronous DA release through Pyk2/ERK/Synapsin I/actin pathway. Our findings provide new insights into the role of GQ1b in neuronal communication, and implicate the potential application of GQ1b in neurological disorders. © 2015 Elsevier Inc.


Shi L.-L.,Peking Union Medical College | Chen B.-N.,Peking Union Medical College | Gao M.,Peking Union Medical College | Zhang H.-A.,Peking Union Medical College | And 3 more authors.
Life Sciences | Year: 2011

Aims: The therapeutic effect of pinocembrin, together with the therapeutic time window, in the transient global cerebral ischemia/reperfusion (I/R) rats was investigated. Main methods: Adult male Sprague-Dawley rats were subjected to global cerebral ischemia for 20 min by four-vessel occlusion. Pinocembrin (1 and 5 mg/kg) was administrated intravenously 30 min before ischemia and 30 min, 2 h, 6 h after reperfusion, respectively. Neurological scores, brain edema and histological examination by Nissl staining were employed to assess the neuronal injury after ischemia and the neuroprotection by pinocembrin. The activities of superoxide dismutase (SOD), myeloperoxidase (MPO) and the content of malondialdehyde (MDA) in brain tissue were tested by colorimetric assays. Alterations of neurotransmitters were determined by a high performance liquid chromatography-electrochemical method. Key findings: Pinocembrin significantly ameliorated neurological deficits and brain edema, and alleviated the degree of hippocampal neuronal loss at 24 h after global cerebral I/R with a broad therapeutic time window. It was found that treatment with pinocembrin reduced the compensatory increase of SOD activity and decreased the MDA level and MPO activity in a dose-dependent manner. The metabolic balance between excitatory and inhibitory amino acids was modulated by pinocembrin treatment. Significance: These findings suggest that pinocembrin provides neuroprotection against global cerebral ischemic injury with a wide therapeutic time window, which may be attributed to its antioxidative, antiinflammatory and antiexcitotoxic effects. © 2011 Elsevier Inc. All rights reserved.


Gao Y.,Peking Union Medical College | Chu S.-F.,Peking Union Medical College | Li J.-P.,Peking Union Medical College | Zhang Z.,Peking Union Medical College | And 10 more authors.
Acta Pharmacologica Sinica | Year: 2015

Aim: Protopanaxtriol (Ppt) is extracted from Panax ginseng Mayer. In the present study, we investigated whether Ppt could protect against 3-nitropropionic acid (3-NP)-induced oxidative stress in a rat model of Huntington's disease (HD) and explored the mechanisms of action. Methods: Male SD rats were treated with 3-NP (20 mg/kg on d 1, and 15 mg/kg on d 2-5, ip). The rats received Ppt (5, 10, and 20 mg/kg, po) daily prior to 3-NP administration. Nimodipine (12 mg/kg, po) or N-acetyl cysteine (NAC, 100 mg/kg, po) was used as positive control drugs. The body weight and behavior were monitored within 5 d. Then the animals were sacrificed, neuronal damage in striatum was estimated using Nissl staining. Hsp70 expression was detected with immunohistochemistry. Reactive oxygen species (ROS) generation was measured using dihydroethidium (DHE) staining. The levels of components in the Nrf2 pathway were measured with immunohistochemistry and Western blotting. Results: 3-NP resulted in a marked reduction in the body weight and locomotion activity accompanied by progressive striatal dysfunction. In striatum, 3-NP caused ROS generation mainly in neurons rather than in astrocytes and induced Hsp70 expression. Administration of Ppt significantly alleviated 3-NP-induced changes of body weight and behavior, decreased ROS production and restored antioxidant enzymes activities in striatum. Moreover, Ppt directly scavenged free radicals, increased Nrf2 entering nucleus, and the expression of its downstream products heme oxygenase-1 (HO-1) and NAD(P)H quinone oxidase 1 (NQO1) in striatum. Similar effects were obtained with the positive control drugs nimodipine or NAC. Conclusion: Ppt exerts a protective action against 3-NP-induced oxidative stress in the rat model of HD, which is associated with its anti-oxidant activity. © 2015 CPS and SIMM All rights reserved 1671-4083/15 $32.00.


Chen X.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Li Y.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Chen W.,The Peoples Hospital Of Guangxi Zhuang Autonomous Region | Nong Z.,Guangxi Institute of Chinese Medicine and Pharmaceutical Science | And 2 more authors.
Neurochemical Research | Year: 2016

Memory decline is characteristic of aging and age-related neurodegenerative disorders. This study was designed to investigate the protective effect of hyperbaric oxygen (HBO) against cognitive impairment induced by d-galactose (d-gal) in mice. d-gal was intraperitoneally injected into mice daily for 8 weeks to establish the aging model. HBO was simultaneously administered once daily. The results indicate that HBO significantly reversed D-gal-induced learning and memory impairments. Studies on the potential mechanisms of this action showed that HBO significantly reduced oxidative stress by increasing superoxide dismutase, glutathione peroxidase, and catalase levels, as well as the total anti-oxidation capability, while decreasing the content of malondialdehyde, nitric oxide, and nitric oxide synthase in the hippocampal CA1 region. HBO also inhibited advanced glycation end-product formation and decreased levels of tumor necrosis factor-α and interleukin-6. Moreover, HBO significantly attenuated d-gal-induced pathological injury in the hippocampus, as well as β-amyloid protein1−42 expression and retained BDNF expression. Furthermore, HBO decreased p16, p21 and p53 gene and protein expression in the hippocampus of d-gal-treated mice. In conclusion, the protective effect of HBO against d-gal-induced cognitive impairment was mainly due to its ability to reduce oxidative damage, suppress inflammatory responses, and regulate aging-related gene expression. © 2016 Springer Science+Business Media New York


Chai L.,Guangxi Institute of Chinese Medicine and Pharmaceutical Science
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To analyze the compositions of the essential oil from the rhizome of Curcuma aromatica in Guangxi. The essential oil from the rhizome of Curcuma aromatica was extrated by steam distillation and analysed by GC-MS. 50 chemical constituents accounting for 93.11% of total content were identified. The main components are eucalyptol (53.86%), neocurdione (9.89%), linalool (4.24%), camphor (3.14%), alpha-terpineol (2.94%) and germacrone (2.89%).


PubMed | Guangxi Institute of Chinese Medicine and Pharmaceutical Science
Type: Journal Article | Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To analyze the compositions of the essential oil from the rhizome of Curcuma aromatica in Guangxi.The essential oil from the rhizome of Curcuma aromatica was extrated by steam distillation and analysed by GC-MS.50 chemical constituents accounting for 93.11% of total content were identified.The main components are eucalyptol (53.86%), neocurdione (9.89%), linalool (4.24%), camphor (3.14%), alpha-terpineol (2.94%) and germacrone (2.89%).

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