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Chen C.,Peoples Hospital of Guangxi Autonomous Region | Chen W.,Peoples Hospital of Guangxi Autonomous Region | Nong Z.,Guangxi Institute of Chinese Medicine and Pharmaceutical Science | Ma Y.,Maternal and Child Hospital of Guangxi Zhuang Autonomous Region | And 2 more authors.
Cellular Physiology and Biochemistry | Year: 2016

Background/Aims: In this study, we examined whether the combination of hyperbaric oxygen (HBO) and diltiazem therapy provided a cardioprotective effect on myocardial ischemia-reperfusion injury (MIRI) rat model. Methods: Sixty healthy Sprague-Dawley rats were randomly divided into sham, IR, diltiazem (5 mg/kg), HBO (0.25 MPa, 60 min) and combination therapy (HBO plus diltiazem) groups. MIRI model was established by ligating the left anterior descending for 30 min, followed by 60 min of reperfusion. Results: The results show that HBO and diltiazem preconditioning significantly improves cardiac function and myocardial infarction area, increases nitric oxide, endothelial nitric oxide synthase and ATPase (Na+-K+-ATPase and Ca2+-Mg2+-ATPase) activity and decreases levels of oxygen stress, myocardial enzymes and endothelin-1. Notably, HBO and diltiazem preconditioning significantly increased Bcl-2 protein expression and decreased Bax protein and caspase-3 mRNA expression. Conclusions: These data indicate that combination therapy protected against heart MIRI by reducing oxygen stress damage, correcting energy metabolism, improving endothelial function and inhibiting cell apoptosis. © 2016 The Author(s).

Xian L.-N.,Nanjing University of Traditional Chinese Medicine | Xian L.-N.,Jiangsu Provincial Institute of Traditional Chinese Medicine | Li Z.-L.,Guangxi Institute of Chinese Medicine and Pharmaceutical Science | Qian S.-H.,Jiangsu Provincial Institute of Traditional Chinese Medicine
Chinese Traditional and Herbal Drugs | Year: 2010

Objective: To study the chemical constituents from the stems of Acanthopanax gracilistylus. Methods: The chemical constituents of the plant were isolated and purified by column chromatography and their structures were elucidated on the basis of physico-chemical properties and spectral data. Results: A new ent-kaurane glycoside, named kaurane acid glycoside A (16α, 17-dihydroxy-ent-kauran-19-oic 19-[β-D-glucopyranosyl-(1→2)-β-D- glucopyranosyl] ester) (1), was isolated from the n-butanol part. Conclusion: Compound 1 is a new one.

Li Z.-L.,China Pharmaceutical University | Li Z.-L.,Jiangsu Province Institute of Traditional Chinese Medicine | Li Z.-L.,Guangxi Institute of Chinese Medicine and Pharmaceutical Science | Qian S.-H.,Jiangsu Province Institute of Traditional Chinese Medicine | And 3 more authors.
Asian Journal of Chemistry | Year: 2012

In this paper, the chemical constituents from the fruits of Cicuta virosa L. var latisecta Celak were isolated and characterized. Isolation and purification were carried out on the column chromatography of silica gel and Sephadex LH-20. Twelve compounds were isolated and identified as rel-(3S, 4S, 5S)-3-[(2R)-2-hydroxydocosanoyl-pentacosanoylaminol]-4-hydroxyl-5-[(4Z) -tetradecane-4-ene]-2, 3, 4, 5-tetrahydrofuran (1a-1d mixture), quercetin (2), isorhamnetin (3), isorhamnetin-3-O-β-D-glucopyranoside (4), (24R)-cycloart-25-ene-3,24-diol (5) and (24S)-cycloart-25-ene-3,24-diol (6) mixture, (6H,7H)-a-spinasterone (7), 2,3-dihydrooenanthetol (8) and n-tricasanoic acid (9).

Zhang Z.,Peking Union Medical College | Chu S.-F.,Hunan University | Mou Z.,Peking Union Medical College | Gao Y.,Peking Union Medical College | And 3 more authors.
Molecular and Cellular Neuroscience | Year: 2016

Growing evidence indicates that GQ1b, one of the gangliosides members, contributes to synaptic transmission and synapse formation. Previous studies have shown that GQ1b could enhance depolarization induced neurotransmitter release, while the role of GQ1b in asynchronous release is still largely unknown. Here in our result, we found low concentration of GQ1b, but not GT1b or GD1b (which were generated from GQ1b by plasma membrane-associated sialidases), evoked asynchronous dopamine (DA) release from both clonal rat pheochromocytoma PC12 cells and rat striatal slices significantly. The release peaked at 2min after GQ1b exposure, and lasted for more than 6min. This effect was caused by the enhancement of intracellular Ca2+ and the activation of Pyk2. Inhibition of Pyk2 by PF-431396 (a dual inhibitor of Pyk2 and FAK) or Pyk2 siRNA abolished DA release induced by GQ1b. Moreover, Pyk2 Y402, but not other tyrosine site, was phosphorylated at the peaking time. The mutant of Pyk2 Y402 (Pyk2-Y402F) was built to confirm the essential role of Y402 activation. Further studies revealed that activated Pyk2 stimulated ERK1/2 and p-38, while only the ERK1/2 activation was indispensable for GQ1b induced DA release, which interacted with Synapsin I directly and led to its phosphorylation, then depolymerization of F-actin, thus contributed to DA release. In conclusion, low concentration of GQ1b is able to enhance asynchronous DA release through Pyk2/ERK/Synapsin I/actin pathway. Our findings provide new insights into the role of GQ1b in neuronal communication, and implicate the potential application of GQ1b in neurological disorders. © 2015 Elsevier Inc.

Yan K.-J.,Guangxi Institute of Chinese Medicine and Pharmaceutical Science | Xu D.-X.,Beijing Normal University | Song Z.-Q.,CAS South China Botanical Garden | Song Z.-Q.,University of Chinese Academy of Sciences
Systematic Botany | Year: 2016

A new species of Rubiaceae, Mycetia fangii, is described and illustrated from Guangxi, South China. The new species morphologically resembles M. mukerjiana but differs in the calyx bearing one short gland on each sinus, the calyx lobes much shorter than the corolla, and shorter bracts and bracteoles. It is one of the species with cauliflorous inflorescences in Mycetia. Further examination of herbarium specimens shows that M. apoensis, M. yatesii, and M. lanceolata (Ridl.) Ridl. (non Miq.) are conspecific with M. cauliflora, the type species of the genus. Thus the three names are here reduced to synonymy of the type species. A key to the species of the genus with cauliflory is provided at the end of this paper. © 2016 by the American Society of Plant Taxonomists.

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