Guangxi Crop Genetic Improvement Laboratory

Nanning, China

Guangxi Crop Genetic Improvement Laboratory

Nanning, China

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He X.,East China Institute of Technology | He X.,Guangxi Crop Genetic Improvement Laboratory | Li L.,East China Institute of Technology | Li L.,Guangxi Crop Genetic Improvement Laboratory | And 12 more authors.
Scientia Horticulturae | Year: 2017

Energy level is closely related to postharvest banana fruit senescence. In this study, high performance liquid chromatography (HPLC) method was developed to determine concentrations of adenylate, i.e. adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP), in postharvest banana fruits. Boiling water extracting adenylate from banana fruits was suitable because of its high extraction rate and stability. HPLC method exhibited good repeatability (variation coefficients of 1.51%–3.42%) and recovery rate (93.9%–97.8%). The correlation coefficients of ATP, ADP and AMP with peak areas in a range of 1–120 mg/L were 0.999965, 0.999995 and 0.999996, respectively. Through analyzing adenylate concentrations and membrane hydrolysis-related enzyme activities (phospholipase D and lipoxygenase, ab. PLD and LOX, two key enzymes catalyzing membrane lipid degradation) in banana fruits stored at different temperatures (7 °C, 14 °C and 25 °C), it could be found that appropriate low temperature (14 °C) delayed fruit senescence by maintaining high energy level. Chilly stress (7 °C) accelerated fruit senescence by declining energy supply level, accompanying by activation of membrane hydrolysis-related enzymes such as PLD and LOX. These results were helpful for elucidating relationship between energy metabolism and senescence regulation during chilling injury in postharvest banana fruits. © 2017 Elsevier B.V.


Sun J.,South China University of Technology | He X.-M.,China Institute of Technology | He X.-M.,Guangxi Crop Genetic Improvement Laboratory | Zhao M.-M.,South China University of Technology | And 5 more authors.
Molecules | Year: 2014

Sugarcane tops were extracted with 50% ethanol and fractionated by petroleum ether, ethyl acetate (EtOAc), and n-butyl alcohol successively. Eight phenolic compounds in EtOAc extracts were purified through silica gel and Sephadex LH-20 column chromatographies, and then identified by nuclear magnetic resonance and electrospray ionization mass spectra. The results showed that eight phenolic compounds from EtOAc extracts were identified as caffeic acid, cis-p-hydroxycinnamic acid, quercetin, apigenin, albanin A, australone A, moracin M, and 5'-geranyl-5,7,2',4'-tetrahydroxyflavone. The antioxidant and nitrite-scavenging capacities of different solvent extracts correlated positively with their total phenolic (TP) contents. Amongst various extracts, EtOAc extracts possessed the highest TP content and presented the strongest oxygen radical absorbance capacity (ORAC), 1,1'-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging capacity, 2,2'-azobis-3-ethylbenthiaazoline-6-sulfonic acid (ABTS) radical-scavenging capacity, ferric reducing antioxidant power (FRAP) and nitrite-scavenging capacity. Thus, sugarcane tops could be promoted as a source of natural antioxidant. © 2014 by the authors; licensee MDPI, Basel, Switzerland.


Sun J.,China Institute of Technology | Sun J.,Guangxi Crop Genetic Improvement Laboratory | Li C.,China Institute of Technology | Nagendra Prasad K.,University of Selangor | And 7 more authors.
Scientia Horticulturae | Year: 2012

A comparative study of membrane deterioration, enzymatic browning and oxidative stress of three litchi cultivars, namely 'Feizixiao', 'Heiye' and 'Xiangli', was carried out. During 6-d storage at room temperature, pericarp browning index and disease rate of 'Feizixiao' were highest. The increasing rate of relative electrolyte leakage of 'Feizixiao' rose most rapidly. The relative phospholipase D activity significantly increased for 'Feizixiao' but insignificantly changed for 'Heiye' and 'Xiangli'. The relative acid phosphatase activity increased for 'Feizixiao' but decreased for 'Heiye' and 'Xiangli'. On day 6, 'Feizixiao' exhibited the lowest relative contents of browning substrates (-)-epicatechin and procyanidin A2. Furthermore, on day 6, 'Heiye' presented the highest relative activities of peroxidase, ascorbate peroxidase and superoxidase dismutase, but 'Feizixiao' possessed weak activities of antioxidant enzymes (especially ascorbate peroxidase). Thus, among three cultivars, membrane integrity of 'Feizixiao' broke down most seriously because of its increasing PLD and ACP activity during 6-d storage. The most browning substrates in 'Feizixiao' were consumed due to conversion into quinones by polyphenoloxidase catalysis. Senescence and deterioration of 'Feizixiao' were most grave under postharvest oxidative stress. © 2012 Elsevier B.V.


You X.,China Institute of Technology | You X.,Guangxi Crop Genetic Improvement Laboratory | Zhang Y.,China Institute of Technology | Zhang Y.,Guangxi Crop Genetic Improvement Laboratory | And 14 more authors.
Molecular Biology Reports | Year: 2014

Longan (Dimocarpus longan Lour.) is a non-climacteric fruit with a short postharvest life. The regulation of phospholipase D (PLD) activity closely relates to postharvest browning and senescence of longan fruit. In this study, a novel cDNA clone of longan PLDδ (LgPLDδ) was obtained and registered in GenBank (accession No. JF791814). The deduced amino acid sequence possessed all of the three typical domains of plant PLDs, a C2 domain and two catalytic HxKxxxxD motifs. The tertiary structure of LgPLDδ was further predicted. The western blot result showed that the LgPLDδ protein was specifically recognized by PLDδ antibody. The Q-RT-PCR (real-time quantitative PCR) result showed that the level of LgPLDδ mRNA expression was higher in senescent tissues than in developing tissues, which was also high in postharvest fruit. The western-blotting result further certified the different expression of LgPLDδ. These results provided a scientific basis for further investigating the mechanism of postharvest longan fruit adapting to environmental stress. © 2014 Springer Science+Business Media.


Sun J.,China Institute of Technology | Sun J.,Guangxi Crop Genetic Improvement Laboratory | Jiang Y.,CAS South China Botanical Garden | Amin I.,University Putra Malaysia | And 5 more authors.
International Food Research Journal | Year: 2011

This research was to determine nutritional composition, essential and toxic elemental content, and major phenolic acid with antioxidant activity in Kadsura coccinea fruit. The results indicated that Kadsura coccinea fruit exhibited the high contents of total protein, total fat, ash and essential elements such as calcium (Ca), ferrum (Fe) and phosphorus (P). The levels of four common toxic elements, i.e. cadmium (Cd), mercury (Hg), arsenic (As) and lead (Pb), were lower than legal limits. By high-performance liquid chromatography (HPLC) analysis, gallic acid was identified as major phenolic acid in peel and pulp tissues. Its contents were no significant difference in both tissues. In comparison with two commercial antioxidants, the major phenolic acid extracted from Kadsura coccinea exhibited stronger 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity and reducing power. Kadsura coccinea fruit is a good source of nutrition and natural antioxidant. It is worthwhile to popularize this exotic fruit around the world.


Sun J.,East China Institute of Technology | Sun J.,Guangxi Crop Genetic Improvement Laboratory | You X.,East China Institute of Technology | You X.,Guangxi Crop Genetic Improvement Laboratory | And 10 more authors.
Postharvest Biology and Technology | Year: 2011

Membrane lipid degradation catalyzed by phospholipase D (PLD) results in postharvest browning and senescence of litchi fruit. The effects of n-butanol, a specific PLD inhibitor, on enzymatic browning and oxidative stress during storage of litchi fruit at room temperature were evaluated. n-Butanol-treated fruit had a lower browning index and disease index than untreated fruit. n-Butanol treatment also decreased PLD activity. As a result, the decompartmentalization of litchi polyphenoloxidase and substrates was reduced. The conversion of substrates (-)-epicatechin and procyanidin A2 into quinones was slowed down and enzymatic browning of litchi pericarp tissues was lower after 6d storage. Additionally, n-butanol-treated fruit possessed significantly lower malondialdehyde contents than untreated fruit after 4d storage. Analysis of antioxidative enzyme activities showed that n-butanol treatment inhibited oxidative stress mainly by maintaining high catalase activity in litchi pericarp tissues. Consequently, senescence of litchi fruit during storage was moderated. © 2011 Elsevier B.V.


Hong K.,Chinese Academy of Sciences | Xie J.,Chinese Academy of Sciences | Zou R.,BGI Shenzen | Zhang E.,East China Institute of Technology | And 4 more authors.
Acta Physiologiae Plantarum | Year: 2015

Banana fruit are highly sensitive to chilling injury (CI), while the effect of different degrees of CI on the subsequent fruit ripening is largely unknown. In the present work, ripening characteristic of banana fruit after storage at 7 °C for 3 days or for 8 days, and expression levels of eight genes associated with ethylene biosynthetic and signaling, including MaACS1, MaACO1, MaERS1, MaERS3, and MaEIL1–4, were investigated. The results showed that banana fruit stored at 7 °C for 8 days exhibited more severe chilling symptoms than those at 7 °C for 3 days. Compared with banana fruit stored at 7 °C for 8 days, which showed abnormal ripening, more decrease in fruit firmness, while higher increase in ethylene production and hue angle were observed in banana fruit stored at 7 °C for 3 days, which could ripening normally. Moreover, gene expression profiles during ripening revealed that ethylene biosynthetic and signaling genes were differentially expressed in peel and pulp of banana fruit after storage at 7 °C for 3 days and 7 °C for 8 days. In the peel of fruit storage at 7 °C for 3 days, expression levels of MaACS1, MaACO1, MaEIL1, and MaEIL2 increased remarkably while MaERS3, MaEIL1, and MaEIL4 were enhanced in the fruit after storage at 7 °C for 8 days. In the pulp, with the exception of MaACO1 and MaERS3, expression levels of other genes did not exhibit a significant difference, between the banana fruit storage at 7 °C for 3 days and 7 °C for 8 days. Taken together, our results suggest that differential expression of ethylene biosynthetic and signaling genes such as MaERS3, MaACO1, and MaEIL2, may be related to ripening behavior of banana fruit with different degrees of CI after cold storage. © 2015, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.


Li L.,East China Institute of Technology | Li L.,Guangxi Crop Genetic Improvement Laboratory | Li J.,East China Institute of Technology | Li J.,Guangxi Crop Genetic Improvement Laboratory | And 12 more authors.
Postharvest Biology and Technology | Year: 2015

Longan (Dimocarpus longan Lour.) is a fruit with a limited postharvest life. In the present study, 2-butanol (a phospholipase D inhibitor) at different concentrations (0.05%, 0.10% and 0.15%) were applied postharvest to longan fruit stored at ambient temperature (25. °C), and their effects on fruit quality and physiological characteristics were investigated. The results showed that the 0.05% 2-butanol treatment had the most positive effects on fruit quality, with treated fruit showing a significant delay in changes in weight, titratable acidity content and total soluble solids content by day 8. The 0.05% 2-butanol treated fruit had the lowest rate of pericarp moisture loss on day 8 and a significantly lower browning index than the control on day 6. It also maintained higher ascorbic acid contents than in the control from day 2. This treatment maintained high contents of phenolics and flavonoids during 8 days storage, and inhibited browning, pulp breakdown and respiration. These results indicate that the phospholipase D inhibitor 2-butanol could be helpful in postharvest quality maintenance of longan fruit. © 2014 Elsevier B.V.


Sun J.,East China Institute of Technology | Sun J.,Guangxi Crop Genetic Improvement Laboratory | Li L.,East China Institute of Technology | Li L.,Guangxi Crop Genetic Improvement Laboratory | And 10 more authors.
Analytical Methods | Year: 2011

Tropical fruits are important sources of phenolics and polysaccharides which are of great benefit to human health and have already been used in foodstuff, drugs and cosmetics. This review attempts to summarize the current research on phenolics and polysaccharides in major tropical fruits with large-scale production. Chemical composition, analytical methods, bioactivities and therapeutic functions of main phenolics and polysaccharides in pericarps, pulps and/or seeds of lychee (Litchi chinensis Sonn.), longan (Dimocarpus longan Lour.), mango (Mangifera indica L.), banana (Musa acuminata L.), pineapple (Ananas comosus L.) and papaya (Carica papaya L.) fruits are elucidated. The future research trends on phenolics and polysaccharides in tropical fruits are also suggested. This review indicates potential applications of phenolics and polysaccharides in tropical fruits, which is an important reference for effectively utilizing these bioactive components in tropical food systems. © 2011 The Royal Society of Chemistry.


He Q.,East China Institute of Technology | He Q.,Guangxi Crop Genetic Improvement Laboratory | Hong K.,Chinese Academy of Sciences | Zou R.,BGI Shenzen | And 4 more authors.
European Food Research and Technology | Year: 2014

Banana fruit are sensitive to chilling stress. This study investigated the possible mechanism of propylene-reducing chilling injury (CI) to postharvest banana fruit. Firmness, chromaticity, ethylene production, CI index, electrolyte leakage, lipoxygenases (LOX) activity, contents of jasmonic acid (JA) and salicylic acid (SA) and the expression of genes MaLOX1, MaACS1 and MaACO1 in fruit were examined. The study shows that the propylene treatment delayed CI symptom development and chromaticity change by 2 days. Meantime, the treatment reduced electrolyte leakage, induced LOX activity in the early stage of cold storage and increased JA content in banana peel. SA immersion after propylene treatment improved the SA content in banana peel, but inhibited the LOX activity and JA content increasing, resulting in CI symptom as serious as that of untreated controls. Expression of genes MaLOX1, MaACS1 and MaACO1 in banana fruit was enhanced by propylene treatment. But no significant differences were observed in firmness, ethylene production, MaLOX1, MaACS1 and MaACO1 expression levels between propylene and propylene SA-treated fruit. These results indicated that the LOX activity increasing and JA burst in the early stage of cold storage may play a key role in tolerance the chilling stress in banana fruit. © 2013 Springer-Verlag Berlin Heidelberg.

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