Guangxi Crop Genetic Improvement and Biotechnology Laboratory

Nanning, China

Guangxi Crop Genetic Improvement and Biotechnology Laboratory

Nanning, China
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Sun J.,China Institute of Technology | Sun J.,Guangxi Crop Genetic Improvement and Biotechnology Laboratory | Zhang E.,China Institute of Technology | Xu L.,CAS South China Botanical Garden | And 3 more authors.
Journal of Agricultural and Food Chemistry | Year: 2010

Longan polyphenoloxidase (PPO) was extracted and partially purified using ammonium sulfate precipitation and dialysis. The PPO characterizations were compared using endogenous substrate (-)-epicatechin and exogenous substrate catechol. The optimal pH and optimal temperature for the PPO activity were different when reacting with both substrates. The addition of ethylenediaminetetraacetic acid disodium salt into both substrate-enzyme systems exhibited the same lowest inhibition of the PPO activity. l-Ascorbic acid and l-cysteine were the best inhibitors to endogenous substrate-enzyme system, while l-ascorbic acid and glutathione were most effective inhibitors to exogenous substrate-enzyme system. Cupric (Cu2+), ferric (Fe3+), and ferrous (Fe2+) ions accelerated the enzymatic-catalyzed reactions of both substrates. Kinetic analysis indicated that longan PPO strongly bound endogenous substrate but possessed a higher catalytic efficiency to exogenous substrate, and moreover, (-)-epicatechin was determined as the optimal substrate for longan PPO. This study is useful to exactly illuminate the enzymatic-catalyzed browning mechanism of postharvest longan fruit. © 2010 American Chemical Society.


Luo C.,Guangxi University | He X.-H.,Guangxi University | He X.-H.,Guangxi Crop Genetic Improvement and Biotechnology Laboratory | Chen H.,Guangxi University | And 5 more authors.
Biochemical Systematics and Ecology | Year: 2011

Two molecular marker systems, SCoT and ISSR were used for identification and genetic comparison analysis of 23 mango germplasm accessions collected within Guangxi province of China. Using 18 selected SCoT primers 158 bands were generated, of which 104 (65.82%) were polymorphic. Eighteen selected ISSR primers amplified 156 bands with 87 (55.77%) being polymorphic. The cultivars of Xiang Ya Mango type and their progeny have high genetic similarity with each other. The 23 cultivars were clustered into two major groups based on the SCoT analysis and three major groups based on the ISSR analysis with UPGMA. These clusters are in accordance with their known origins and main phenotypic characteristics. Our results indicated that the SCoT analysis better represents the actual relationships than ISSR analysis, although both analyses give similar results. The results also demonstrate that the SCoT marker system is useful for identification and genetic diversity analysis of mango cultivars. © 2011 Elsevier Ltd.


Sun J.,Guangxi Academy of Agricultural science | Sun J.,Guangxi Crop Genetic Improvement and Biotechnology Laboratory | You Y.,Yantai University | Garcia-Garcia E.,University Miguel Hernández | And 3 more authors.
Food Chemistry | Year: 2010

Polyphenoloxidase (PPO) was partially purified from chufa corms through ammonium sulphate precipitation and dialysis. Biochemical properties of chufa PPO were analysed using exogenous substrate catechol. Optimal pH and temperature for PPO activity were 5 and 45 °C. Ethylenediaminetetraacetic acid disodium salt and l-cysteine could not inhibit the PPO activity. However, sodium thiosulphate pentahydrate exhibited the strongest inhibiting effect, followed by ascorbic acid and anhydrous sodium sulphite. Except for K+, other metal ions such as Zn2+, Cu2+, Fe3+, Ca2+, Fe2+ and Na+ accelerated the enzymatic reaction between catechol and PPO. Kinetic analysis showed that the apparent Km and Vmax values were around 10.77 mM and 82 units/ml min. In addition, (-)-gallocatechin gallate, (-)-epicatechin gallate and (+)-catechin gallate isolated and identified from chufa corms were supposed to be the potential endogenous PPO substrates due to their ortho-diphenolic or pyrogallolic structures. These polyphenols might be catalysed by PPO, resulting in the browning of chufa corms after fresh-cut processing. © 2009 Elsevier Ltd. All rights reserved.


Luo C.,Guangxi University | He X.-H.,Guangxi University | He X.-H.,Guangxi Crop Genetic Improvement and Biotechnology Laboratory | Hu Y.,Guangxi University | And 3 more authors.
Gene | Year: 2014

Differential display is a powerful technique for analyzing differences in gene expression. Oligo-dT cDNAstart codon targeted marker (cDNA-SCoT) technique is a novel, simple, cheap, rapid, and efficient method for differential gene expression research. In the present study, the oligo-dT anchored cDNA-SCoT technique was exploited to identify differentially expressed genes during several stress treatments in mango. A total of 37 primers combined with oligo-dT anchor primers 3side amplified approximately 150 fragments of 150. bp to 1500. bp in length. Up to 100 fragments were differentially expressed among the stress treatments and control samples, among which 92 were obtained and sequenced. Out of the 92 transcript derived fragments (TDFs), 70% were highly homologous to known genes, and 30% encoded unclassified proteins with unknown functions. The expression pattern of nine genes with known functions involved in several abiotic stresses in other species was confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) under cold (4. °C), salinity (NaCl), polyethylene glycol (PEG, MW 6000), and heavy metal treatments in leaves and stems at different time points (0, 24, 48, and 72. h). The expression patterns of the genes (TDF4, TDF7, TDF23, TDF45, TDF49, TDF50, TDF57, TDF91 and TDF92) that had direct or indirect relationships with cold, salinity, drought and heavy metal stress response were analyzed through qRT-PCR. The possible roles of these genes are discussed. This study suggests that the oligo-dT anchored cDNA-SCoT differential display method is a useful tool to serve as an initial step for characterizing transcriptional changes induced by abiotic stresses and provide gene information for further study and application in genetic improvement and breeding in mango. © 2014 Elsevier B.V.


Luo C.,Guangxi University | He X.-H.,Guangxi University | He X.-H.,Guangxi Crop Genetic Improvement and Biotechnology Laboratory | Chen H.,Guangxi University | And 2 more authors.
Genetic Resources and Crop Evolution | Year: 2012

Mango (Mangifera indica L.) is one of the most economically grown fruits in the tropical and subtropical areas around the world. In the present study, start codon targeted (SCoT) markers were employed to investigate the genetic diversity of 73 mango accessions obtained from Guangxi province, China. A total of 275 bands were amplified by thirty-four SCoT primers, of which 203 (73.82%) were polymorphic. Genetic similarity between accessions was in the range of 66.2-94.2% with an average of 78.8%. The observed highest genetic similarity value (94.2%) was found between 'Ren Mian Mango' and 'Hong Hua Mango', the observed lowest genetic similarity value (66.2%) was found between'Xia Mao Xiang Mango' and 'India No. 15'. These coefficients were utilized to construct a dendrogram using the unweighted pair group of arithmetic means. All the accessions were grouped into four (A, B, C, D) clusters and correspond well with their geographical origin and their known history. These results have an important implication for mango's rue germplasm characterization, improvement, management and conservation. © 2011 Springer Science+Business Media B.V.


Liu Z.-L.,Guangxi University | Luo C.,Guangxi University | Dong L.,Guangxi University | Van Toan C.,Guangxi University | And 3 more authors.
Gene | Year: 2014

The Rab family, the largest branch of Ras small GTPases, plays a crucial role in the vesicular transport in plants. The members of Rab family act as molecular switches that regulate the fusion of vesicles with target membranes through conformational changes. However, little is known about the Rab5 gene involved in fruit ripening and stress response. In this study, the MiRab5 gene was isolated from stress-induced Mangifera indica. The full-length cDNA sequence was 984. bp and contained an open reading frame of 600. bp, which encoded a 200 amino acid protein with a molecular weight of 21.83. kDa and a theoretical isoelectric point of 6.99. The deduced amino acid sequence exhibited high homology with tomato (91% similarity) and contains all five characteristic Rab motifs. Real-time quantitative RT-PCR analysis demonstrated that MiRab5 was ubiquitously expressed in various mango tree tissues at different levels. The expression of MiRab5 was up-regulated during later stages of fruit ripening. Moreover, MiRab5 was generally up-regulated in response to various abiotic stresses (cold, salinity, and PEG treatments). Recombinant MiRab5 protein was successfully expressed and purified. SDS-PAGE and western blot analysis indicated that the expressed protein was recognized by the anti-6-His antibody. These results provide insights into the role of the MiRab5 gene family in fruit ripening and stress responses in the mango plant. © 2014 Elsevier B.V.


Luo C.,Guangxi University | He X.-H.,Guangxi University | He X.-H.,Guangxi Crop Genetic Improvement and Biotechnology Laboratory | Chen H.,Guangxi University | And 2 more authors.
Biologia Plantarum | Year: 2013

Actin is the most abundant protein in eukaryotic cells and is a key cytoskeletal component controlling cell morphology and motility. In this study, four MiACT genes were isolated from mango by homological cloning and designated as MiACT1, MiACT4, MiACT7, and MiACT9. Sequence alignments and phylogenetic analysis demonstrated that the four MiACT genes of mango were highly similar to each other at the nucleotide and amino acid levels. All of four MiACT proteins showed high similarity to the known actin proteins from other species. Reverse transcription polymerase chain reaction revealed that the four MiACT genes were constitutively and stably expressed in all organs tested. Application of plant growth regulators and four stress treatments had a remarkable effect on the expression of MiACT4, MiACT7 and MiACT9, whereas expression of MiACT1 was unresponsive. In contrast, the expression profiles of the four MiACT genes were not regulated by diurnal rhythms. Moreover, the expression of MiACT1 was not affected by heavy metal treatments and the transcript level of MiACT1 was rather stable in different days during the post-harvest period either under treatment or not. Our results suggest that the four actin genes play important roles throughout the entire life cycle of mango; the constitutively and stably expressed MiACT1 is the best candidate as an internal standard for differential gene expression analysis in mango. © 2013 Springer Science+Business Media Dordrecht.


Luo C.,Guangxi University | Dong L.,Guangxi University | He X.H.,Guangxi University | He X.H.,Guangxi Crop Genetic Improvement and Biotechnology Laboratory | And 3 more authors.
Journal of Horticultural Science and Biotechnology | Year: 2014

Abscisic acid-, stress-, and ripening-induced (ASR) proteins are low molecular weight plant-specific proteins that play important roles in plant responses to abiotic and biotic stresses, including drought, cold, salinity, wounding, and pathogen attack. In addition, they function in plant development and in fruit ripening, and have a regulatory role in the cross-talk between primary carbon metabolism and hormone signalling. In a previous study, an ASR gene fragment was obtained from stress-induced leaves and stems of Mangifera indica L. using the cDNA-Start Codon Targeted marker (SCoT) technique. Using rapid amplification of cDNA ends (RACE), we have obtained the full-length cDNA sequence of the mango ASR gene, named MiASR.MiASR contained an open reading frame (ORF) of 516 nucleotides, encoding a predicted protein of 172 amino acid residues. Sequence analysis showed that MiASR displayed similarity to all previously characterised ASR gene sequences and its product shared the major characteristics of ASR proteins found in other plants. However, the nucleotide and predicted amino acid sequences of MiASR were significantly different from those of ASR genes and ASR proteins in other species. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analyses indicated that MiASR was expressed in leaves and stems, but some tissue-specific differences and regulatory changes were observed in response to low temperature, salinity, or polyethylene glycol treatment.These results showed that differential expression of the MiASR gene has an important role in the response of mango to stress. Levels of expression of MiASR decreased continuously during the harvest period in unstressed control mangoes. These levels were negatively regulated and maintained at low values in all hot water, citric acid, oxalic acid, or hot water plus 1-methylcyclopropene (1-MCP)-treated fruit.The MiASR protein may therefore be involved in mango fruit ripening and post-harvest physiology.


Luo C.,Guangxi University | He X.-H.,Guangxi University | He X.-H.,Guangxi Crop Genetic Improvement and Biotechnology Laboratory | Chen H.,Guangxi University | And 2 more authors.
Biochemical Systematics and Ecology | Year: 2010

Genetic variation and relationships among 47 mango germplasm and 3 relative species from Guangxi province in China, were analyzed using Start Codon Targeted (SCoT) markers. Using 33 selected SCoT primers 273 bands were generated with an average of 8.27 bands per primer among the 50 accessions, of which 208 (76.19%) were polymorphic. Genetic relationships estimated using the SM similarity coefficient generated values between different pairs of accessions that varied from 0.531 to 0.923 with an average of 0.782. These coefficients were utilized to construct a dendrogram using the UPGMA. All 50 accessions were basically classified into six clusters and correspond well with their recorded pedigrees. The results will provide much more useful information for the management of germplasm and will also be useful to improve the current breeding strategies. The results also demonstrate that the SCoT marker system is useful for identification and genetic diversity analysis of mango cultivars. © 2010 Elsevier Ltd.


Saxena K.B.,Indian International Crops Research Institute for the Semi Arid Tropics | Ravikoti V.K.,Indian International Crops Research Institute for the Semi Arid Tropics | Dalvi V.A.,Guangxi Crop Genetic Improvement and Biotechnology Laboratory | Pandey L.B.,Maharashtra Hybrid Seed Company | Gaddikeri G.,Maharashtra Hybrid Seed Company
Journal of Heredity | Year: 2010

Pigeonpea [Cajanus cajan (L.) Millsp.] is a unique food legume because of its partial (20-30%) outcrossing nature, which provides an opportunity to breed commercial hybrids. To achieve this, it is essential to have a stable male-sterility system. This paper reports the selection of a cytoplasmic-nuclear male-sterility (CMS) system derived from an interspecific cross between a wild relative of pigeonpea (Cajanus sericeus Benth. ex. Bak.) and a cultivar. This male-sterility source was used to breed agronomically superior CMS lines in early (ICPA 2068), medium (ICPA 2032), and late (ICPA 2030) maturity durations. Twenty-three fertility restorers and 30 male-sterility maintainers were selected to develop genetically diverse hybrid combinations. Histological studies revealed that vacuolation of growing tetrads and persistence of tetrad wall were primary causes of the manifestation of male sterility. Genetic studies showed that 2 dominant genes, of which one had inhibitory gene action, controlled fertility restoration in the hybrids. The experimental hybrids such as TK 030003 and TK 030009 in early, ICPH 2307 and TK 030625 in medium, and TK 030861 and TK 030851 in late maturity groups exhibited 30-88% standard heterosis in multilocation trials. © The American Genetic Association. 2010. All rights reserved.

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