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Lin C.-R.,CAS Institute of Botany | Liu Y.,CAS Institute of Botany | Nong D.-X.,Guangxi Botanical Garden of Medicinal Plants | Kono Y.,Academia Sinica, Taiwan | Peng C.-I.,Academia Sinica, Taiwan
Botanical Studies | Year: 2013

Background Aspidistra crassifila Yan Liu & C.-I Peng, a new species of the Asparagaceae from Guangxi Zhuang Autonomous Region, China, is described and illustrated. Results The new species is similar to A. subrotata Y. Wan & C. C. Huang in the perianth lobes triangular-lanceolate and horizontally spreading, but differs by the perianth campanulate, lobes with appendages at base, stamens 6-8 mm long, filaments enlarged, anthers adnate to perianth tube, connectives extended and upcurved. The chromosome number of the new species was determined to be 2n = 38, and the karyotype was formulated as 2n = 22m2SC+4sm+12st. Conclusion A careful study of the literature, herbarium specimens and living plants, both in the wild and in cultivation in the experimental greenhouse, support the recognition of the new species Aspidistra crassifila, which is described herein. Aspidistra crassifila is currently known only from Shiwandashan Mountains, which lie in southern Guangxi. A line drawing, color plates and a distribution map are given for the new species to aid in identification. © 2013 Lin et al.

Li W.,Hanshan Normal University | Hao J.,Guangxi Botanical Garden of Medicinal Plants | Xiao Y.,Shantou University
Archives of Pharmacal Research | Year: 2013

Ten lupeol dicarboxylic acid monoester derivatives as new potent antitumor agents were synthesized and evaluated for in vitro antitumor activities against A549, LAC, HepG2 and HeLa cell lines. Among them, compounds 1-5 showed excellent antitumor activities against all tested tumor cell lines and compounds 6-10 exhibited high activities against A549, HepG2 and HeLa cells, exceeded lupeol, lupanol and doxorubicin. Compound 2 displayed the highest potent antitumor activities with IC50 values of 5.78 μM against A549 cell, 2.38 μM against LAC cell, 6.14 μM against HepG2 cell and 0.00842 μM against HeLa cell. © 2013 The Pharmaceutical Society of Korea.

Guo X.-Y.,Guangxi Botanical Garden of Medicinal Plants | Guo X.-Y.,CAS Institute of Botany | Huang Z.-Y.,CAS Institute of Botany | Xu A.-C.,China Jiliang University | Zhang X.-S.,CAS Institute of Botany
Forestry | Year: 2011

Effects of a 30-day flooding period on some morphological and physiological changes and on relative growth rate (RGR) of 13 hybrid poplar clones, belonging to four different parentages i.e. T × N, T × D, D × N and N × E (with T = Popolus trichocarpa, N = P. nigra, D = P. deltoids and E = P. euramericana) were studied under controlled conditions. Responses of the 13 clones to flooding differed, and they varied with duration of flooding period. Stomatal conductance was not reduced in any of the plants during the first few days of flooding. However, in trees of some clones, stomata opened and then closed as flooding was prolonged. Net photosynthesis rates (Pn) of flooded trees of some clones decreased during flooding, but in some flooded clones it returned to the value of the control following a period of decrease. RGR was reduced by flooding in 10 clones and was correlated with reduction of Pn. Other responses to flooding included formation of adventitious roots and relative amount of carbon allocated to the above- and below-ground tissues. Based on the dendrogram from the hierarchical cluster analysis, the 13 clones tested could be divided into two groups. Clones 58-280, DN-34, 311-93, DN-70, Simplot, R-419 and OP-367 were grouped into a cluster with high tolerance to flooding; clones 309-74, PC-1, DN-14273, 195-529, NE-353 and R-247 into a cluster with low tolerance to flooding. © Institute of Chartered Foresters, 2010. All rights reserved.

Ouyang K.,South China Agricultural University | Li J.,South China Agricultural University | Huang H.,Guangxi Botanical Garden of Medicinal Plants | Que Q.,South China Agricultural University | And 2 more authors.
Biotechnology and Biotechnological Equipment | Year: 2014

Plant tissues contain abundant polysaccharides, phenolic compounds and other metabolites, which makes it difficult to isolate high-quality RNA from them. In addition, Neolamarckia cadamba contains large quantities of other components, particularly RNA-binding alkaloids, which makes the isolation even more challenging. Here, we describe a concise and efficient RNA isolation method that combines the cetyltrimethyl ammonium bromide (CTAB) and Plant RNA Kit (Omega) protocols. Gel electrophoresis showed that RNA extracted from all tissues, using this protocol, was of good integrity and without DNA contamination. Furthermore, the isolated RNA was of high purity, with an A260/A280 ratio of 2.1 and an A260/A230 ratio of >2.0. The isolated RNA was also suitable for downstream applications, such as reverse transcription-polymerase chain reaction (RT-PCR) and quantitative RT-PCR (RT-qPCR). The RNA isolation method was also efficient for recalcitrant plant tissues. © 2014 The Author(s). Published by Taylor & Francis.

Wei R.-C.,Guangxi Botanical Garden of Medicinal Plants | Wei R.-C.,Peking Union Medical College | Bai L.-H.,Guangxi Botanical Garden of Medicinal Plants
Chinese Traditional and Herbal Drugs | Year: 2015

To evaluate the genetic background of triploid female and diploid male strains of Siraitia grosvenorii and provide the biological reference for good varieties breeding of seedless S. grosvenorii. Inter simple sequence repeats (ISSR) marker was developed to analyze the genetic background among 28 samples of S. grosvenorii, and cluster analysis and double principal coordinate analysis were revealed by the NTSYS-pc software and GenAIEx software, respectively. Out of 100 ISSR primers selected, 13 primers were used for amplification and a total of 131 unambiguous bands were obtained, among which 99 (PPB = 75.57%) were polymorphic. The results of cluster analysis and double principal coordinate analysis showed that there was a certain rich of genetic background in triploid female and diploid male strains of S. grosvenorii. But the genetic similarity coefficients of majority were bigger and the genetic distance was closer. The complexity of the genetic background in triploid female and diploid male strains of S. grosvenorii is lower and germplasm innovation strategies should be carried out to enrich the genetic background of the parents of seedless S. grosvenorii. ©, 2015, Chinese Traditional and Herbal Drugs. All right reserved.

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