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Ji G.,Guangdong Provincial Institute of Public Health | Ji G.,U.S. Center for Disease Control and Prevention | Ji G.,Sun Yat Sen University | Zhang Y.,Jinan University | And 5 more authors.
PLoS ONE | Year: 2012

Genistein, the major isoflavone in soybean, was recently reported to exert beneficial effects in metabolic disorders and inflammatory diseases. In the present study, we investigated the effects and mechanisms of a dietary concentration of genistein on the inflammatory response in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Our results demonstrated that genistein effectively inhibited the LPS-induced overproduction of tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6), as well as LPS-induced nuclear factor kappa B (NF-κB) activation. In addition, the data also showed that genistein prevented LPS-induced decrease in adenosine monophosphate-activated protein kinase (AMPK) phosphorylation. These effects were obviously attenuated by an AMPK inhibitor. Taken together, our results suggest that the dietary concentration of genistein is able to attenuate inflammatory responses via inhibition of NF-κB activation following AMPK stimulation. The data provide direct evidence for the potential application of low concentrations of genistein in the prevention and treatment of inflammatory diseases. © 2012 Ji et al.


Tang M.Q.,Guangxi Botanical Garden of Medicinal Plant
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

The virus isolate H was identified by molecular biology,it was collected from Abelmoschus manihot plant showing leaf curl,yellow vein symptoms in Guangxi Botanical Garden of Medicinal Plant. The virus isolate H was observed in electron micrograph, and conformed detected by PCR using universal primer pair for the genus Geminivirus. The results indicated that all sequences homologous to the specific fragment belonged to the genus Begomovirus of the family Geminiviridae. There was the highest similarity shared 95% homology at nucleotide between the specific fragment and DNA-A of Emilia yellow vein virus isolates. These findings suggested that there was geminiviridea in Abelmoschus manihot, and the disease probably caused by Emilia yellow vein virus.


Ma G.-X.,Peking Union Medical College | Xu X.-D.,Peking Union Medical College | Cao L.,Peking Union Medical College | Yuan J.-Q.,Guangxi Botanical Garden of Medicinal Plant | And 2 more authors.
Planta Medica | Year: 2012

Five new cassane-type diterpenes, neocaesalpin AA (1), neocaesalpin AB (2), neocaesalpin AC (3), neocaesalpin AD (4) and neocaesalpin AE (5), were isolated from Caesalpinia minax together with three known compounds, 12α-methoxyl,5α,14β-dihydroxy-1α,6α, 7β-triacetoxycass-13(15)-en-16,12-olide (6), spirocaesalmin (7) and magnicaesalpin (8). Their structures were elucidated based on 1D and 2D NMR, MS and CD analyses. Compounds 1-6 were tested against Hela, HCT-8, HepG-2, MCF-7 and A549 cancer cells and showed moderate cytotoxicity with ICvalues from 18.4 to 83.9 M. © Georg Thieme Verlag KG Stuttgart · New York.


Qin L.Y.,Guangxi Botanical Garden of Medicinal Plant
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To identify the pathogens what caused of root rot, it can provide method of theoretical gist of integrated pest management of these kinds of diseases in the future. Pathogens from rotten root of Sophora tonkinensis were isolated by tissue isolation. Their morphological characteristics were observed and rDNA-ITS sequence were sequenced, then analyzed by Blast in GenBank. Round colony in PDA medium. The aerial mycelium was thin, white, light gray and yellowish brown eustroma was on the surface of material. The surface of base material was flesh. Large number of small conidia ware oval, kidney-shaped, 8-16 microm x 2.5 -4 microm. And the large conidia just like Matt spore type, which had 3 to 5 septums. The length of rDNA-ITS of the fungi was 553 bp, which the ITS region sequences compared with the sequence of Fusarium solani (accession number: AB518683.1, AB470903.1, AB369488.1, AJ608989.1, GQ365154.1, EF152426.1), and Fusarium oxysporum (accession number: GQ922558.1, GQ922559.1, DQ452447.1) homology reached 99%. Combination of two identification methods,it arrived at the cause of root rot pathogen fungi was Fusarium solani.


Lan M.S.,Guangxi Botanical Garden of Medicinal Plant
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To study the chemical constituents of the ethyl acetate extract from Blumea aromatica. Column chromatographic techniques were used for the isolation and purification. Spectroscopic techniques were used for the identification of structures. Seven compounds were isolated from the ethyl acetate extract of 80% alcohol extract of the whole plant, and their structures were identified as xanthoxylin(1), dibutyl phthalate(2), luteolin-3',4',7-trimethyl ether(3), rubiadin(4), eriodictyol(5), kaempherol(6), luteolin 7,3'-dimethyl ether(7). All the compounds are isolated from this plant for the first time. Among them, compounds 2,4 and 7 are isolated from this genus for the first time.

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