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Gao X.-M.,Anhui Agricultural University | Lin Y.-R.,Anhui Agricultural University | Huang H.-Y.,Guangxi Academy of Forestry | Hou C.-L.,Capital Normal University
Mycological Progress | Year: 2013

Cathay silver fir, Cathaya argyrophylla, is a national first-class protected species in China and honored as a "living fossil" of the plant kingdom. Recently, a needle cast of Cathay silver fir of the Guangxi Zhuang Autonomous Region, China was investigated. This pathogen not only reduces the host ornamental value, but seriously affects the growth of this endangered plant. In this paper, the needle cast, formerly misidentified as Lophodermium pinastri, is identified as Lophodermium cathayae. L. cathayae differs morphologically from L. pinastri in many aspects, but particularly in anamorph morphology. An analysis of the internal transcribed spacer of rDNA shows that L. cathayae is closely related to L. pinastri and that further study is required in order to update the circumscription of L. pinastri. The fungal diversity conservation for new species L. cathayae is discussed. © 2012 German Mycological Society and Springer.


Cui B.-W.,Guizhou University | Fan F.-H.,Guizhou University | Ding G.-J.,Guizhou University | Yang Z.-Q.,Guangxi Academy of Forestry | Wen X.-P.,Guizhou University
Forest Research | Year: 2016

Objective: To develop new molecular markers suitable for Pinus massoniana. Method: Based on the conservative region of RT (reverse transcriptase) sequences of Ty1-copia and Ty3-gypsy type retrotransposon, the IRAP-PCR system for P. massoniana was established and examined with 12 individuals. Result: Of 42 IRAP primers, 29 gave stable and polymorphic amplification profiles, thus yielded 227 bands, among which 207 were polymorphic, accounting for 91.19% of the total. The average Observed Number of Alleles, Effective Number of Alleles, Nei's Genetic Diversity and Shannon Index of Diversity in the twelve P. massoniana gemplasms were 1.9119±0.2841, 1.4680±0.2882, 0.2911±0.1449 and 0.4472±0.1953, respectively. The cultivated types and clones could be effectively distinguished by primers P-12, P-15 or R-1, and all the genotypes might be clarified by P-2, which was labeled as the core primer for tested germplasms. The coefficient of the 12 germplasms ranged from 0.46 to 0.69, and with the threshold of 0.57, all the genotypes were grouped into three subclusters by the UPGMA. Conclusion: The IRAP marker technology established may effectively facilitate the identification of genetic relationship in P. massoniana germplasms. © 2016, Chinese Academy of Forestry. All right reserved.


Lin G.-S.,Guangxi University | Ma C.-H.,Guangxi University | Duan W.-G.,Guangxi University | Cen B.,Guangxi University | And 2 more authors.
Holzforschung | Year: 2014

This work is aiming at the preparation of new bioactive compounds from a-pinene as starting material. To this purpose, the intermediates (disubstituted phenyl acylamino thioureas, class G) were cyclized to dithiadiazoles (class H). The intermediates and target compounds were analyzed by Fourier transform infrared, 1H nuclear magnetic resonance (NMR), 13C NMR, and electrospray ionization-mass spectrometry and elemental analysis. The bioassay experiments showed that the compounds G and H have herbicidal, fungicidal, and plant growth-regulating activities. The compounds 2,2'-dimethylphenyl thiadiazole and 3,3'-dimethylphenyl thiadiazole exhibited a growth inhibition activity of 72% and 68% against the root of rape (Brassica campestris L.) at a concentration of 100 μg ml-1 (60-79% inhibition level). The compound 4,4'-dichlorophenyl acylamino thiourea displayed an inhibition activity of 75% against Physalospora piricola at a concentration level of 50 μg ml-1 (60-79% inhibition level). © 2014 Walter de Gruyter GmbH, Berlin/Boston.


Wang J.-Y.,Central South University of forestry and Technology | Tan X.-F.,Central South University of forestry and Technology | Zeng Y.-L.,Central South University of forestry and Technology | Long H.-X.,Central South University of forestry and Technology | And 2 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2014

3-Hydroxyacyl-CoA dehydratase (HCD) is a kind of protein that is the dehydrateion of the 3-hydroxyacyl- CoA to an enoyl-CoA. In this paper, a full-length cDNA of 3-hydroxyacyl-CoA dehydratase in Camellia oleifera 'Huashuo' seeds was cloned using reverse transcription PCR (RT-PCR), RACE technique, and basing' transcriptome and expression profiling database of C. oleifera seed. The full length cDNA was 1 145 bp, and the gene was named CoHCD (GenBank No. KJ910336). The sequence had an open reading frame of 666 bp, encoding 221 amino acids rich in hydrophobic residues (48.9%), showing a hydrophilic protein. Sequence analysis showed that molecular mass of the CoHCD protein was 25.2 kDa with a theoretical pI of 9.4. CoHCD had four transmembrane domains and contained a protein tyrosine phosphatase motif "HGXXGXXRS" that was common to the PTPLA gene families. The expression vectors of CoHCD were constructed successfully, and the BL21(DE3) bacteria harboring the pET30a-CoHCD was induced to express the about 25 kDa target protein. The relative expression abundance of CoHCD at 5 different developmental stages of seed were analyzed using RT-PCR. The CoHCD gene expression was up-regulated during the seed developmental stages (with the highest point occurring in middle September and high-efficiency expression in August to October), which was consistent with the accumulation of the lipid synthesis of C. oleifera seeds.


Li F.,Guangxi University | Li F.,Guilin Medical University | Mo Q.,Guangxi University | Duan W.,Guangxi University | And 4 more authors.
Medicinal Chemistry Research | Year: 2014

In an attempt to search for natural product-based insecticidal agents, a series of novel dehydroabietic acid derivatives bearing 1,3,4-thiadiazole moiety were designed and synthesized. Their structures were characterized by IR, MS, 1H-NMR, 13C-NMR, and elemental analysis. The insecticidal activities against cotton bollworm (Helicoverpa armigera), corn borer (Ostrinia nubilalis Hübner), and diamondback moth (Plutella xylostella (L.)) were evaluated. The bioassay test showed that some of the target compounds exhibited excellent insecticidal activities at the concentration of 200 μg/ml; compound 3a had the best mortality rate of 90.0 and 80.0 % against H. armigera and O. nubilalis Hübner, respectively. © 2014 Springer Science+Business Media New York.


Lin G.-S.,Guangxi University | Dong S.-Q.,Guangxi University | Duan W.-G.,Guangxi University | Cen B.,Guangxi University | And 2 more authors.
Holzforschung | Year: 2014

In search of new bioactive compounds, a series of maleated rosin-based dithiourea compounds were designed and synthesised with rosin as starting material. All the title compounds were characterised by Fourier transform infra-red spectroscopy (FT-IR), proton nuclear magnetic resonance spectroscopy (1H NMR), and 13C nuclear magnetic resonance spectroscopy (13C NMR). The preliminary bioassay showed that the target compounds exhibited a certain fungicidal activity against Physalospora piricola at a concentration of 50 μg ml-1 and a certain growth herbicidal activity against the root of rape (Brassica campestris L.) at a concentration of 100 μg ml-1. © 2014 by Walter de Gruyter Berlin/Boston 2014.


A new genus and four new species of eriophyoid mites of the tribe Acaricalini (Acari, Eriophyoidea) from the Longlin and Heng counties, Guangxi Zhuang Autonomous Region, China are described and illustrated: Chiacap hyllisa syzygii n. gen., n. sp. infesting Syzygium sp. (Myrtaceae), Protumescoptes laurifolii n. sp. infesting Geniconthus laurifolius (Roxb.) Hook.f. (Asclepiadaceae), Litaculus tremae n. sp. infesting Trema angustifolia (Planch.) Blume (Ulmaceae) and Paracaphyllisa montani n. sp. infesting Gnetum montanum Markgr. (Gnetaceae). All species described here are vagrants on the undersurface of host leaves. A key to the genera of Acaricalini in China is provided. © Publications Scientifiques du Muséum national d'Histoire naturelle.


Jiang Y.,Central South University of forestry and Technology | Jiang Y.,Guangxi Academy of Forestry | Li Z.H.,Central South University of forestry and Technology | Zhu J.Y.,Guangxi Academy of Forestry | And 4 more authors.
Genetics and Molecular Research | Year: 2015

Castanopsis hystrix is one of the most important and dominant species in evergreen broad-leaved forests in subtropical China. However, the population of this species undergone severe decline because of deforestation over the past 2 decades. For both conservation and forestry management, it is essential to develop molecular markers for C. hystrix. We identified 11 microsatellite loci in 2 wild populations. The number of alleles ranged from 3-11, with an average of 6.45 alleles per locus. The observed and expected heterozygosities ranged from 0.640-0.960 and from 0.676-0.910, respectively. ©FUNPEC-RP.


PubMed | Guangxi Academy of Forestry and Central South University of forestry and Technology
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Castanopsis hystrix is one of the most important and dominant species in evergreen broad-leaved forests in subtropical China. However, the population of this species undergone severe decline because of deforestation over the past 2 decades. For both conservation and forestry management, it is essential to develop molecular markers for C. hystrix. We identified 11 microsatellite loci in 2 wild populations. The number of alleles ranged from 3-11, with an average of 6.45 alleles per locus. The observed and expected heterozygosities ranged from 0.640-0.960 and from 0.676-0.910, respectively.


Huang X.-R.,Guangxi Academy of Forestry | Pang S.-L.,Guangxi Academy of Forestry | Shen W.-H.,Guangxi Academy of Forestry | Hou Y.-R.,Guangxi Academy of Forestry | He F.,Guangxi Academy of Forestry
Chinese Journal of Applied Ecology | Year: 2016

Community invaded by Praxelis clematidea in karst mountainous area of Pingguo, Guangxi Province was investigated including 4 land types, i.e. abandoned cropland, young plantation, roadside and shrub. Meta analysis, analysis of covariance, linear mixed effect model and recursive partitioning were used to analyze their herb diversity and richness in relation to environmental variables. The results showed that summary effects were positive and significant, effects of groups were positive, effect of abandoned cropland was negative, and effects of other three land types were positive, and shrub had high weight in the analysis. Analysis of covariance indicated that importance value of P. clematidea, distance to driveway and soil were the main numeric factors influencing herb diversity and herb species richness, and plots invaded had higher herb diversity and richness than those uninvaded. Land types had extremely significant impacts on herb diversity but had nothing on herb species richness. Linear mixed effect model partitioned the explained variances of random effects, factor P. clematidea present or absent and residuals respectively contributed 50.9% and 49.1% in Shannon model, while 39.0% and 61.0% in richness model, groups and land types contributed zero to the random effects. P. clematidea invasion could be classified into 5 levels, and modest invasion caused higher herb diversity and richness. When P. clematidea importance value was more than 23.6%, the critical threshold, both herb diversity and richness declined. © 2016, Science Press. All right reserved.

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