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Tan X.-F.,Central South University of forestry and Technology | Wang J.-Y.,Central South University of forestry and Technology | Long H.-X.,Central South University of forestry and Technology | Zeng Y.-L.,Central South University of forestry and Technology | And 3 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2014

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid (FFA) and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl- CoAs, FFAs and CoASH. In this paper, state trial oil-tea variety 'Huashuo' as material, a full-length cDNA of acyl-CoA thioesterase genes from Camellia oleifera was isolated and cloned by RACE technology and reverse transcription PCR (RT-PCR). This gene was named CoACOT (GenBank accession number KJ910339) and the full-length cDNA was found to be 1 588 bp. CoACOT had an open reading frame of 1 164 bp, encoding 387 amino acids. CoACOT contained a conserved sequences of "VVREGEAGDGVYFIWDG" in cNMP binding domain and a highly conserved triple peptides of "SKL" in the C-terminal, belonging to a peroxysome protein. The expression vectors of CoACOT were constructed successfully, and the recombinant vector of pET30a-Co-ACOT was induced to expressed the about 44 kDa target product in the BL21 (DE3) bacteria. The real-time quantitive PCR showed the expression level of CoACOT was the lowest in seed expanding stage, and then remained almost a relatively stable higher level after up-regulation in the lipid synthesis. The results showed CoACOT, as a auxiliary gene, may regulate and maintain the physiological balance in lipid synthesis of C. oleifera seeds. ©, 2014, Science Press. All right reserved. Source

Wang J.-Y.,Central South University of forestry and Technology | Tan X.-F.,Central South University of forestry and Technology | Zeng Y.-L.,Central South University of forestry and Technology | Long H.-X.,Central South University of forestry and Technology | And 2 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2014

3-Hydroxyacyl-CoA dehydratase (HCD) is a kind of protein that is the dehydrateion of the 3-hydroxyacyl- CoA to an enoyl-CoA. In this paper, a full-length cDNA of 3-hydroxyacyl-CoA dehydratase in Camellia oleifera 'Huashuo' seeds was cloned using reverse transcription PCR (RT-PCR), RACE technique, and basing' transcriptome and expression profiling database of C. oleifera seed. The full length cDNA was 1 145 bp, and the gene was named CoHCD (GenBank No. KJ910336). The sequence had an open reading frame of 666 bp, encoding 221 amino acids rich in hydrophobic residues (48.9%), showing a hydrophilic protein. Sequence analysis showed that molecular mass of the CoHCD protein was 25.2 kDa with a theoretical pI of 9.4. CoHCD had four transmembrane domains and contained a protein tyrosine phosphatase motif "HGXXGXXRS" that was common to the PTPLA gene families. The expression vectors of CoHCD were constructed successfully, and the BL21(DE3) bacteria harboring the pET30a-CoHCD was induced to express the about 25 kDa target protein. The relative expression abundance of CoHCD at 5 different developmental stages of seed were analyzed using RT-PCR. The CoHCD gene expression was up-regulated during the seed developmental stages (with the highest point occurring in middle September and high-efficiency expression in August to October), which was consistent with the accumulation of the lipid synthesis of C. oleifera seeds. Source

Gao X.-M.,Anhui Agricultural University | Lin Y.-R.,Anhui Agricultural University | Huang H.-Y.,Guangxi Academy of Forestry | Hou C.-L.,Capital Normal University
Mycological Progress | Year: 2013

Cathay silver fir, Cathaya argyrophylla, is a national first-class protected species in China and honored as a "living fossil" of the plant kingdom. Recently, a needle cast of Cathay silver fir of the Guangxi Zhuang Autonomous Region, China was investigated. This pathogen not only reduces the host ornamental value, but seriously affects the growth of this endangered plant. In this paper, the needle cast, formerly misidentified as Lophodermium pinastri, is identified as Lophodermium cathayae. L. cathayae differs morphologically from L. pinastri in many aspects, but particularly in anamorph morphology. An analysis of the internal transcribed spacer of rDNA shows that L. cathayae is closely related to L. pinastri and that further study is required in order to update the circumscription of L. pinastri. The fungal diversity conservation for new species L. cathayae is discussed. © 2012 German Mycological Society and Springer. Source

Li F.,Guangxi University | Li F.,Guilin Medical University | Mo Q.,Guangxi University | Duan W.,Guangxi University | And 4 more authors.
Medicinal Chemistry Research | Year: 2014

In an attempt to search for natural product-based insecticidal agents, a series of novel dehydroabietic acid derivatives bearing 1,3,4-thiadiazole moiety were designed and synthesized. Their structures were characterized by IR, MS, 1H-NMR, 13C-NMR, and elemental analysis. The insecticidal activities against cotton bollworm (Helicoverpa armigera), corn borer (Ostrinia nubilalis Hübner), and diamondback moth (Plutella xylostella (L.)) were evaluated. The bioassay test showed that some of the target compounds exhibited excellent insecticidal activities at the concentration of 200 μg/ml; compound 3a had the best mortality rate of 90.0 and 80.0 % against H. armigera and O. nubilalis Hübner, respectively. © 2014 Springer Science+Business Media New York. Source

Lin G.-S.,Guangxi University | Dong S.-Q.,Guangxi University | Duan W.-G.,Guangxi University | Cen B.,Guangxi University | And 2 more authors.
Holzforschung | Year: 2014

In search of new bioactive compounds, a series of maleated rosin-based dithiourea compounds were designed and synthesised with rosin as starting material. All the title compounds were characterised by Fourier transform infra-red spectroscopy (FT-IR), proton nuclear magnetic resonance spectroscopy (1H NMR), and 13C nuclear magnetic resonance spectroscopy (13C NMR). The preliminary bioassay showed that the target compounds exhibited a certain fungicidal activity against Physalospora piricola at a concentration of 50 μg ml-1 and a certain growth herbicidal activity against the root of rape (Brassica campestris L.) at a concentration of 100 μg ml-1. © 2014 by Walter de Gruyter Berlin/Boston 2014. Source

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