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Wu T.,Guangxi Teachers Education University | Wu T.,Guangxi Academy of Fishery science | Xiu L.,Guangxi Teachers Education University | Yang J.,Guangxi Teachers Education University
Ichthyological Exploration of Freshwaters | Year: 2016

Liniparhomaloptera macrostoma, new species, is described from Hunan Province, south China. It is distinguished from its congers by having 8 branched dorsal-fin rays, more lateral line scales (82-100), relatively wider mouth and lower lip separated into two parts. © 2016 by Verlag Dr. Friedrich Pfeil, München, Germany.

PubMed | Chinese Academy of Sciences, University of Louisiana at Lafayette, National Marine Environmental Monitoring Center, Shanxi Agricultural University and Guangxi Academy of Fishery science
Type: | Journal: Aquatic toxicology (Amsterdam, Netherlands) | Year: 2016

Chromium (Cr) is an essential metal and a nutritional supplement for both human and agricultural uses. It is also a pollutant from a variety of industrial uses. These uses can lead to elevated Cr levels in aquatic environments, where it can enter and affect aquatic organisms. Its accumulation and subsequent effects in fish have received relatively little attention, especially for chronic exposure. In the present study, Japanese medaka were chronically exposed to dissolved or dietary Cr(VI) for 3 months. Cr accumulation in liver, gills, intestine, and brain was evaluated. Effects on the antioxidant system, nervous system (acetylcholinesterase, AChE), digestive system (-glucosidase, -Glu), and tissue histology (liver and gills) were also assessed. Cr accumulation was observed in the intestine and liver of fish exposed to Cr-contaminated brine shrimp. However, chronic dissolved Cr exposure led to significant Cr accumulation in all organs tested. Analysis of the subcellular distribution of Cr in medaka livers revealed that 37% of the Cr was present in the heat stable protein fraction. The dissolved Cr exposure had pronounced effects on the antioxidant system in the liver, with an elevated ratio of reduced glutathione/oxidized glutathione (GSH/GSSG) and decreases in GSH and glutathione S-transferase (GST). The -Glu activity in the intestine was significantly inhibited. In addition, Cr exposure caused histopathological alterations in the gills and liver. In general, the effects of dietary Cr were relatively minor, possible due to the much lower accumulation in the fish. Our results imply that Japanese medaka accumulate Cr mainly via uptake of dissolved Cr(VI).

PubMed | Chinese Academy of Sciences, Guangxi Academy of Fishery Science, University of Louisiana at Lafayette, National Marine Environmental Monitoring Center and Inner Mongolia University for Nationalities
Type: | Journal: Aquatic toxicology (Amsterdam, Netherlands) | Year: 2016

Aquatic organisms take up selenium from solution and from their diets. Many questions remain regarding the relative importance of selenium accumulation from these sources and resulting effects in benthic invertebrates. The present study addressed the toxicity and accumulation of Se via dissolved and dietary exposures to three different Se species, in the freshwater oligochaete Lumbriculus variegatus. Worms were exposed to 20g/g dry weight of selenite (Se(IV)), selenate (Se(VI)), or seleno-l-methionine (Se-Met) in their diet (sediment) or to 15g/L dissolved Se in water-only exposures. While the dissolved and sediment Se levels differed greatly, such levels may co-occur at a Se-contaminated site. Se accumulation, worm population growth, lipid peroxidation (as TBARS), and Na(+)/K(+)-ATPase activity were quantified at the end of the 2-week exposure. The sediment Se-Met exposure caused 100% mortality, while worm densities were reduced by the other exposures except the Se(VI) one. Se bioaccumulation was generally higher for the sediment-Se exposure than the dissolved-Se ones, and was higher for Se(IV) than Se(VI) in the dissolved-Se exposure but not the sediment-Se one. The Se accumulation was highest for Se-Met. The oligochaetes that accumulated Se had higher levels of lipid peroxidation and reduced Na(+)/K(+)-ATPase activity. The present studys findings of differences in Se accumulation and toxicity for the three Se species, with effects generally but not exclusively a function of Se body burdens, underscore the need for research on these issues in invertebrates. Moreover, the results imply that the dietary uptake route is the predominant one for Se accumulation in L. variegatus.

PubMed | Guangxi Academy of Fishery Science, Dalian Ocean University, Liaoning Academy of Environmental science, CAS Shenyang Institute of Applied Ecology and 2 more.
Type: | Journal: Environmental science and pollution research international | Year: 2017

The Hun River is a major tributary of the Liao River in the northeast area of China and provides drinking water for 23 million local residents. This study was designed to assess the severity of metal contamination in the Hun River and the potential use of indigenous organisms (the fish Zacco platypus and the snail Radix swinhoei) as biomonitors of metal contamination. Water, sediment, and the native fish and snails were collected at four sampling sites that differed in their physicochemical characteristics and their contamination levels. The samples were analyzed for Cd, Cr, Cu, Ni, Pb, and Zn by ICP-MS. The results showed that although the overall potential ecological risks of the metals were low at our sampling sites, Cd posed a noteworthy ecological risk. Strong correlations were obtained between Cd concentrations in the organisms and in the environment. The results indicated that Z. platypus and R. swinhoei can be useful biomonitoring species for assessing Cd contamination. Biomonitoring with the snail may be most effective when focused on the gonad/digestive tissue (because of the high metal accumulation there), but further work is needed to confirm this.

PubMed | Chinese Academy of Fishery Sciences, Changsha University and Guangxi Academy of Fishery science
Type: | Journal: Fish & shellfish immunology | Year: 2016

Common carp (Cyprinus carpio) is a widespread freshwater fish and economically important species in China and other East Asian countries. Recent studies suggest that insulin can alter the expression of immune genes and, thus, can be regarded as an immunomodulatory hormone. To understand the mechanism of the immune response to insulin, we performed a comparative RNA-seq transcriptome analysis using livers from common carp injected with insulin (5g/g bodyweight) or saline as a control. After filtering the low-quality reads and removing the adaptors, the clean raw reads were assembled into 60,421 unigenes with mean length of 746.81bp. Furthermore, 37,107 unigenes were annotated based on homology after blast search in public databases. Differentially expressed genes were identified using the fragments per kb per million fragments method and EdgeR software. In total, 782 differentially expressed genes were found. Thereinto, 444 and 338 genes were upregulated and downregulated, respectively, in the insulin-injected group. A Gene Ontology analysis indicated that these genes were concentrated in glucose metabolism, hormone secretion, andimmune system processes. Moreover, 153 enriched KEGG pathways were associated with the differentially expressed genes, including the Toll-like receptor (TLR) and nuclear factor kappa beta (NF-B) signaling pathways. Signal transducer and activator of transcription 1 (10.56-fold), TLR3 (0.089-fold), activator protein-1 (0.007-fold), tumor necrosis factor- (0.139-fold), and macrophage inflammatory protein-1 (0.038-fold) expression were significantly changed after the insulin injection. This study characterized the profile of genes expression response to insulin in common carp liver for the first time and provided new insight into understanding the molecular mechanism of insulin as an immunomodulatory hormone.

PubMed | Guangxi Academy of Fishery science, Lingnan Normal University and Xinjiang Normal University
Type: | Journal: Marine genomics | Year: 2016

Ink ejection is one of the most important defense mechanisms against external stimuli for pharaoh cuttlefish (Sepia pharaonis). The molecular changes during this process remain unknown. To understand the transcriptome changes after ink ejection by brief pressing, two cDNA libraries of pharaoh cuttlefish, from the inkjet group and control group were sequenced using Illumina HiSeq 2000. A total of 9,255,502,440nt bases were obtained and by de novo assembly, 73,298 unigenes were generated, which first provided numerous expressed sequence tags from pharaoh cuttlefish. By comparing the expression levels between the two groups, we identified 7064 up-regulated and 2024 down-regulated genes after ink ejection. These differentially-expressed genes included genes related to immunity, cancer, and blood coagulation, which indicated the various effects after ink ejection by brief pressing. These results provide new valuable resources for functional genomic and genetic studies on pharaoh cuttlefish.

PubMed | Shanghai Ocean University, Shenzhen Entry Exit Inspection and Quarantine Bureau and Guangxi Academy of Fishery Science
Type: | Journal: Veterinary immunology and immunopathology | Year: 2016

Toll-like receptors (TLRs) are a class of pattern recognition receptors (PRRs) that recognize pathogen associated molecular patterns (PAMPs) and play an important role in the antiviral response. To determine the effect of common carp TLR9 (CcTLR9) overexpression on the expression of down-stream interferon associated immune factors in epithelioma papulosum cyprini (EPC) cells, may provide useful information for the further investigation on the anti-virus effect mediated by TLR9 in fish. In this study, we constructed an overexpression vector, pEGFP-N1-CcTLR9, by cloning the CcTLR9 gene and inserting it into an expression vector pEGFP-N1. Both plasmids DNA of pEGFP-N1 and pEGFP-N1-CcTLR9 were transfected into EPC cells, and the expression of IRF3, IRF7, ISG15, Mx1, PKR and Viperin mRNA at 0, 6, 12, 24, 48 and 72h post-transfection were determined by real-time quantitative PCR (Q-PCR). Overexpression of the CcTLR9 gene in EPC cells upregulates the expression of IRF3, IRF7, ISG15, Mx1, PKR, and Viperin mRNA, and this was more significant for Viperin, ISG15, and IRF7, and least significant for PKR. Thus, fish TLR9 activates IRF7 signaling to induce I-IFN secretion and the subsequent upregulation of IFN-stimulated genes.

PubMed | Guangxi Academy of Fishery Science, Guangxi University and Guangxi Aquaculture and Animal Husbandry School
Type: | Journal: Biosensors & bioelectronics | Year: 2016

A colloidal gold immunochromatographic strip was developed for rapid detection of Streptococcus agalactiae (S. agalactiae) infection in tilapia. The monoclonal antibodies (mAb) 4C12 and 3A9 were used to target S. agalactiae as colloidal gold-mAb conjugate and captured antibody, respectively. The colloidal gold immunochromatographic strip was assembled via routine procedures. Optimal pH and minimum antibody levels in the reaction system for gold colloidal-mAb 4C12 conjugation were pH 7.4 and 18g/mL, respectively. Optimal concentrations of the captured antibody 3A9 and goat anti-mouse antibody were 0.6mg/mL and 2mg/mL, respectively. The sensitivity of the strip for detecting S. agalactiae was 1.510

He A.Y.,Guangxi Academy of Fishery science | Xiu L.H.,Guangxi Teachers Education University | Yang J.,Guangxi Teachers Education University
Journal of Applied Ichthyology | Year: 2016

Studied were the length–weight relationships (LWRs) of 599 fish specimens covering four families, nine genera and nine species (Sinibrama macrops, Acrossocheilus parallens, Microphysogobio fukiensis, Squalidus wolterstorffi, Pseudohemiculter dispar, Tachysurus fulvidraco, Hemibagrus macropterus, Pseudobagrus crassilabris and Siniperca kneri) collected in the Liujiang River, a tributary of the Pearl River, in March 2014, September 2014 and March 2015. The b value varied between 2.385 (P. crassilabris) and 3.178 (P. dispar), with the mean b = 2.85 at P < 0.001 for all nine species. © 2016 Blackwell Verlag GmbH

PubMed | Guangxi Academy of Fishery science and Central South University
Type: | Journal: Fish & shellfish immunology | Year: 2016

Toll-like receptors (TLRs) are central players in the innate immune system in response to a wide range of pathogen infection. Among various TLRs, TLR4 plays a key role in recognition of bacterial lipopolysaccharides (LPS). In the present study, we identified and characterized a novel TLR4 homologue (maTLR4b) in blunt snout bream (Megalobrama amblycephala) which was significantly distinct from previously reported M.amblycephala TLR4 (tentatively named maTLR4a). The results showed that the complete cDNA sequence of maTLR4b was 3261bp with an open reading frame encoding a polypeptide of 820 amino acids, and that its genomic sequence was 3793bp, which had 3 exons. Structurally, the deduced maTLR4b protein showed a typical TLR domain architecture, including a signal peptide, eight leucine-rich repeats (LRRs) in the extracellular region, a transmembrane domain, and a Toll-Interleukin 1 receptor (TIR) domain in the cytoplasmic region. Phylogenetic analysis revealed that all TLR4s from teleost fish formed a monophyletic clade. Both maTLR4a and maTLR4b were divided into two distinct branches, and showed the highest level of similarity with the grass carp TLR4.2 and TLR4.4 homologue, respectively. MaTLR4b was constitutively expressed in all healthy tissues tested although at different levels. After LPS stimulation, the expression levels were significantly up-regulated in spleen, and peaked at 4h between maTLR4a and maTLR4b, but with a distinct and complementary expression patterns. Taken together, these results suggested that maTLR4b is indeed a functional homologue of TLR4 in other species, which may play vital role in innate immune.

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