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Chen J.,Shanxi Agricultural University | Cao J.,Shanxi Agricultural University | Luo Y.,Guangxi Academy of Fishery science | Xie L.,CAS Shenyang Institute of Applied Ecology | And 3 more authors.
Acta Histochemica | Year: 2014

Chronic exposure to fluoride can result in a variety of adverse effects in fish. Previously we indicated that high fluoride caused damage and apoptosis in the kidneys of the common carp, Cyprinus carpio. In this study, the effects of fluoride on the expression and localization of ERK and p-ERK proteins in the ERK signaling pathway were determined using Western blotting and immunohistochemical methods in the kidneys of carp exposed to 0, 40, 80, 120 mg/L fluoride, respectively. Western blotting analysis found that compared with the controls, the levels of ERK1 and ERK2 proteins were relatively unchanged in fluoride-exposed fish, while p-ERK1 and p-ERK2 protein levels decreased significantly with the increased fluoride concentrations. The immunohistochemical analysis found the proteins of ERK and p-ERK were predominantly localized in the cytoplasm of epithelial cells in the renal tubules of C. carpio. Compared with the control group, the levels of ERK protein were relatively constant, yet the levels of p-ERK protein and p-ERK/ERK ratio were reduced with fluoride exposure dose. These findings indicate that the renal damage in carp exposed to fluoride is mediated via the ERK pathway. Fluoride exposure could inactivate ERK, inhibit the expression of p-ERK protein, and induce renal damage in C. carpio. © 2014 Elsevier GmbH. All rights reserved.

Huang J.,Guangxi Normal University | Wei Q.,Guangxi Normal University | Zhang W.,Guangxi Normal University | Zhang Z.,Guangxi Normal University | Liao Y.-Z.,Guangxi Academy of Fishery science
Synthesis and Reactivity in Inorganic, Metal-Organic and Nano-Metal Chemistry | Year: 2016

Treatment of two kinds of unsymmetrical bis-heterocyclic ligands bearing benzimidazole moiety, 2-[2-(1,2,4-triazole-1-yl)ethyl]-1H-benzimidazole (L1) and 2-[2-(1,2,3-benzotriazole-1-yl)ethyl]-1H-benzimidazole (L2), with Co(II) chloride, resulted in two new complexes, [Co3 (L2)4Cl6]·2H2O (1) and [Co(L1)Cl2]2(2), with different aggregating patterns. Two complexes were characterized by single-crystal X-ray determinations. In the solid state, L1assumes an anti conformation in compound 1, whereas L2 exists as a gauche conformer in compound 2. Complex 1 reveals a 1-D double-stranded chain structure consisting of both octahedrally and tetrahedrally coordinated Co(II) ions. Complex 2 is a mononuclear tetrahedral Co(II) species, which was hydrogen bonded with each other to give a 1-D linear chain. Copyright © 2016 Taylor & Francis Group, LLC.

He A.Y.,Guangxi Academy of Fishery science | Xiu L.H.,Guangxi Teachers Education University | Yang J.,Guangxi Teachers Education University
Journal of Applied Ichthyology | Year: 2016

Studied were the length–weight relationships (LWRs) of 599 fish specimens covering four families, nine genera and nine species (Sinibrama macrops, Acrossocheilus parallens, Microphysogobio fukiensis, Squalidus wolterstorffi, Pseudohemiculter dispar, Tachysurus fulvidraco, Hemibagrus macropterus, Pseudobagrus crassilabris and Siniperca kneri) collected in the Liujiang River, a tributary of the Pearl River, in March 2014, September 2014 and March 2015. The b value varied between 2.385 (P. crassilabris) and 3.178 (P. dispar), with the mean b = 2.85 at P < 0.001 for all nine species. © 2016 Blackwell Verlag GmbH

He A.,Guangxi Academy of Fishery science | Huang W.,Tiandong Fishery Technique Popularization Station | He Y.,CAS Shanghai Institute of Applied Physics | Yang J.,Guangxi Teachers Education University
Ichthyological Exploration of Freshwaters | Year: 2015

Cophecheilus brevibarbatus, new species, is described from Longbu Spring in Tiandong County, Guangxi, South China. It is distinguished from the only congener C. bameti, by its short barbels, relatively anterior insertion of dorsal and pelvic fins, and pharyngeal teeth in three rows with five teeth on the inner row. © 2015 by Verlag Dr. Friedrich Pfeil, München, Germany.

Zhong H.,Guangxi University | Zhong H.,Guangxi Academy of Fishery science | Xiao J.,Guangxi Academy of Fishery science | Chen W.,Shanghai Ocean University | And 7 more authors.
Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology | Year: 2014

Growth hormone (GH) and its receptors are critical regulators of somatic growth and metabolism. It has been shown in mammals that the methylation of cytosines within the GH promoter plays a key role in regulating transcripts expression. In the present study, the GH, GHR1 and GHR2 proximal promoters were identified and the methylation levels of these genes in corresponding tissues were assayed. The results suggested that significant arising of GH putative promoter methylation levels in pituitary was observed in females compared with males. However, no such sex-specific changes were found in GHR1 and GHR2 promoters. The GH mRNA expression also was influenced by GH promoter methylation levels in pituitary, which resulted in the higher growth rate of Nile tilapia males. Meanwhile, the methylation levels of GH putative promoter were negatively correlated with growth rate as well as mRNA expression of GH. Furthermore, the methylation of specific E-Box CpG site is also negatively related to the mRNA expression of GH in pituitary. Taken together, our data provide an epigenetic mechanism of explicating the sex duality in phenotypic plasticity of growth rate in male and female of Nile tilapia. © 2014 Elsevier Inc.

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