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Zhou Y.,Changsha University | Zhou Y.,Guangxi Academy of Fisheries Science | Hao G.,Changsha University | Zhong H.,Guangxi Academy of Fisheries Science | And 4 more authors.
Genetics and Molecular Research | Year: 2015

Protein use is crucial for the ovulation and spawning of fish. Currently, limited information is available regarding the expression of protein absorption factors during the breeding seasons of teleosts and thus how various proteins involved in this process is not well-understood. The expression of CDX2, CREB, gluatamate dehydrogenase, LAT2, aminopeptidase N, PepT1, and SP1 were significantly elevated from the non-breeding season to the breeding season in female goldfish, and all proteins except PepT1 and SP1 were elevated in male goldfish. Injection of human chorionic gonadotropin upregulated the expression of all proteins except for aminopeptidase N in female goldfish and SP1 in male goldfish, suggesting a luteinizing hormone-inductive effect on protein absorption factors. Protein use in the intestine is increased during the breeding seasons as a result of increased luteinizing hormone. © FUNPEC-RP. Source


Zhou Y.,Guangxi Academy of Fisheries Science | Zhou Y.,Changsha University | Zhong H.,Guangxi Academy of Fisheries Science | Xiao J.,Guangxi Academy of Fisheries Science | And 4 more authors.
Genes and Genomics | Year: 2016

It has been shown that piRNA is the largest class of small non-coding RNA in germline cells of animals which plays key roles in transposons regulation and transcriptional activities. In the present study, piRNAs from two small RNA libraries including ovary and testis of Nile tilapia were identified and characterized. By length and k-mer based small RNA prediction algorithm, 279,059 and 583,230 small RNA reads were confirmed as piRNA from ovary and testis, respectively. The identified piRNAs showed evolutionarily conserved characterization, such as uridine bias in the 5′ ends. The 142,961 and 296,775 piRNAs from ovary and testis were mapped to the draft assembly of the tilapia genome, respectively. Both ovary and testis piRNAs were enriched from linkage (LG)6 and LG7. Meanwhile, the even distribution of +strand and −strand suggested the Ping–pong pathway (a double-displacement reaction of +strand and −strand) hypothesis. These piRNAs were derived from the upstream −2 kb and downstream +2 kb as well as gene regions which suggested a regulatory function on transcription activities. In gene regions, abundant piRNAs were derived from 5′UTR, 3′UTR and CDS. Furthermore, we characterized the differentially expressed piRNAs between ovary and testis. In total, 1979 and 2453 piRNAs were significantly higher and lower expressed in ovary compared to that in testis, respectively. Thereinto, the most concentrated up-regulate and down-regulate piRNAs were both from serine/threonine–protein kinase PIM genes of different transcripts. These findings will be helpful to facilitate studies on the piRNAs regulation on genes during gonad development of teleosts. © 2016 The Genetics Society of Korea and Springer-Science and Media Source


Xiao J.,Guangxi Academy of Fisheries Science | Xiao J.,Guangxi University | Xiao J.,CAS Kunming Institute of Zoology | Zhong H.,Guangxi Academy of Fisheries Science | And 11 more authors.
PLoS ONE | Year: 2014

MicroRNAs (miRNAs) are endogenous non-coding small RNAs which play important roles in the regulation of gene expression by cleaving or inhibiting the translation of target gene transcripts. Thereinto, some specific miRNAs show regulatory activities in gonad development via translational control. In order to further understand the role of miRNAmediated posttranscriptional regulation in Nile tilapia (Oreochromis niloticus) ovary and testis, two small RNA libraries of Nile tilapia were sequenced by Solexa small RNA deep sequencing methods. A total of 9,731,431 and 8,880,497 raw reads, representing 5,407,800 and 4,396,281 unique sequences were obtained from the sexually mature ovaries and testes, respectively. After comparing the small RNA sequences with the Rfam database, 1,432,210 reads in ovaries and 984,146 reads in testes were matched to the genome sequence of Nile tilapia. Bioinformatic analysis identified 764 mature miRNA, 209 miRNA-5p and 202 miRNA-3p were found in the two libraries, of which 525 known miRNAs are both expressed in the ovary and testis of Nile tilapia. Comparison of expression profiles of the testis, miR-727, miR-129 and miR-29 families were highly expressed in tilapia ovary. Additionally, miR-132, miR-212, miR-33a and miR-135b families, showed significant higher expression in testis compared with that in ovary. Furthermore, the expression patterns of the miRNAs were analyzed in different developmental stages of gonad. The result showed different expression patterns were observed during development of testis and ovary. In addition, the identification and characterization of differentially expressed miRNAs in the ovaries and testis of Nile tilapia provides important information on the role of miRNA in the regulation of the ovarian and testicular development and function. This data will be helpful to facilitate studies on the regulation of miRNAs during teleosts reproduction. © 2014 Xiao et al. Source


Zhao Y.Z.,Guangxi Academy of Fisheries Science | Chen X.L.,Guangxi Academy of Fisheries Science | Zeng D.G.,Guangxi Academy of Fisheries Science | Yang C.L.,Guangxi Academy of Fisheries Science | And 2 more authors.
Genetics and Molecular Research | Year: 2015

The Rab protein family belongs to a superfamily of ras-like GTP-binding proteins. Rab proteins regulate many steps of membrane trafficking. In this study, three Rab family members, Rab5B, Rab6A, and Rab7, designated LvRab5B, LvRab6A, and LvRab7, were cloned from Litopenaeus vannamei. The full-length cDNA sequences of LvRab5B, LvRab6A, and LvRab7 were 1383, 873, and 767 nucleotides in length and they encoded proteins of 211, 212, and 205 amino acids, respectively. Using qRT-PCR, the mRNA expression levels of the three proteins were determined in the hepatopancreas of L. vannamei at different stages after infectious hypodermal and hematopoietic necrosis virus and white spot syndrome virus challenge. The results indicated that the mRNA expression levels of LvRab5B, LvRab6A, and LvRab7 were all significantly up-regulated after virus injection, suggesting that these genes may play essential roles in the immune response to viral infection in shrimp. © FUNPEC-RP. Source


Zeng D.,Guangxi Academy of Fisheries Science | Peng M.,Guangxi Academy of Fisheries Science | Chen X.,Guangxi Academy of Fisheries Science | Yang C.,Guangxi Academy of Fisheries Science | And 2 more authors.
Crustaceana | Year: 2015

Peroxiredoxin (Prx) is an important peroxidase that can protect organisms against various oxidative stresses. In this study, a member of Prx family, designated LvPrx, was cloned from Litopenaeus vannamei. Sequence and phylogenetic analyses indicated that LvPrx belongs to the 2-Cys Prx (Prx IV) isoform. The recombinant LvPrx protein was constructed and expressed in Escherichia coli, and the purified LvPrx proteins were shown to reduce H2O2 in vitro in the presence of dithiothreitol, indicating that LvPrx is a functional peroxiredoxin. Using qRT-PCR, the mRNA expression levels of LvPrx were determined in the haemocytes of L. vannamei at different stages after being challenged with WSSV and TSV at different doses. The results showed that the expression levels of LvPrx were significantly up-regulated (P<0.05) during 4-24 h after both WSSV and TSV challenge, suggesting that LvPrx may participate in the shrimp's immune response to viral infection. © Koninklijke Brill NV, Leiden, 2015. Source

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