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Zhang G.,Chinese Academy of Agricultural Sciences | Zhang G.,Guangxi Sugarcane Branch of the National Sugar Crop Improvement Center | Li Y.-R.,Chinese Academy of Agricultural Sciences | Li Y.-R.,Guangxi Sugarcane Branch of the National Sugar Crop Improvement Center | And 11 more authors.
Sugar Tech | Year: 2010

Random amplified polymorphic DNA (RAPD)-PCR was employed to analyze the genetic diversity within 89 Saccharum spontaneum accessions collected from five different ecological regions of Guangxi Province, China. Of the 87 bands generated by nine RAPD primers, 96.6% were polymorphic. A substantial degree of genetic diversity was found among the locally collected S. spontaneum accessions. Pairwise genetic distance coefficients between accessions ranged from 8.6% (between accessions 87-10 and Yunge {circled digit three}) to 74.2% (between accessions 87-31 and 85-53) with an average of 39.9%. Based on these genetic distance coefficients, the 89 S. spontaneum accessions were clustered into four groups irrespective of their ecological regions using the Cluster program of SAS (V8) software. Information from the present study would be useful for the conservation of locally collected S. spontaneum germplasm and exploitation of its genetic potential during sugarcane crossing program. © 2010 Society for Sugar Research & Promotion.

Song X.,Guangxi University | Huang X.,Chinese Academy of Agricultural Sciences | Tian D.,Guangxi Academy of Agriculture science | Yang L.,Guangxi University | And 3 more authors.
Life Science Journal | Year: 2013

Sugarcane smut (U. scitaminea Syd.), one of the most severe sugarcane diseases, could lead to considerable yield loss and reduction in cane quality. In order to provide useful information for developing rational strategies to control smut at early stage of disease development, a proteomic approach was used to analyze proteins differently expressed during the early interaction between sugarcane seedling and pathogen. Tissue cultured plantlets of sugarcane cultivar ROC22 with 6-7 leaves were inoculated with teliospores suspension at the apical portion using a syringe. Total proteins were extracted from uninoculated control and smut-inoculated plantlets 3 d post-inoculation, separated by 2-DE system, and the protein spots with differential expression in intensity (p<0.05) were analyzed and tentatively identified using MALDI-TOF-TOF/MS/MS. Eighteen protein spots were successfully identified, of which 15 were up-regulated and 3 were down-regulated. Based on their putative biochemical role, the proteins were classified into 7 categories, including defense response, metabolism, photosynthesis, signal transduction, protein processing, cell growth/division and unclassified. These proteins were related to oxidative burst, photorespiration and PAs synthesis and so on. The information obtained in this research provides the first proteomic analysis of the responses of sugarcane seedlings to U. scitaminea inoculation at the early stage of pathogen invasion. © 2013. Marsland Press, Zhengzhou University.

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