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Liu Y.,Jiangnan University | Sun J.,Jiangsu University | Rao S.,Yangzhou University | Su Y.,Jiangnan University | And 4 more authors.
Food and Chemical Toxicology | Year: 2013

Se-polysaccharide from Catathelasma ventricosum (SPC-2) was purified by DEAE-52 and Sephadex G-100 column chromatography. The average size of SPC-2 was 1.6×105 Da, and it was mainly composed of glucose (87.4%) with the conformation of β-pyran ring. The branched structure of SPC-2 was proved intuitively by atomic force microscope (AFM). The antidiabetic potential of SPC-2 was tested in STZ-induced diabetic mice. After STZ-induced diabetic mice being administered of SPC-2 for 30days, SPC-2 treatment significantly reduced the levels of malondialdehyde (MDA) and low-density lipoprotein cholesterol (LDL-C) that were increased by the STZ treatment. Further, the SPC-2 treatment led to increased activity of antioxidant enzymes in liver and kidney and high-density lipoprotein cholesterol (HDL-C) that were decreased by the STZ. The results of histopathology also showed SPC-2 protected tissues (pancreas, liver and kidney) against peroxidation damage and maintained tissue integrity. © 2013 Elsevier Ltd.

Liu Y.,Sichuan Agricultural University | Li C.,Jiangnan University | Luo X.,Jiangnan University | Han G.,Sichuan Agricultural University | And 6 more authors.
Journal of Agricultural and Food Chemistry | Year: 2015

This is the first report concerning the selenium enrichment of Catathelasma ventricosum mycelia. The selenium-containing proteins present in selenium-enriched mycelia (Se-MC) were identified using size-exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS). The selenium-containing amino acids liberated by hydrolysis of these proteins were identified using anion exchange-ICP-MS. Se-MC was found to contain selenoproteins with molecular weights ranging from 1.7 to 60.5 kDa. The main selenium-containing amino acids within them were selenomethionine and selenocysteine. Furthermore, Se-MC possessed excellent antihyperglycemic and antioxidant properties. Se-MC normalized biochemical parameters like insulin level, blood glucose level, body weight, and antioxidant enzyme activity in streptozocin-induced diabetic mice. It also inhibited the α-amylase and α-glucosidase activities present in in vitro gastric and intestinal models. In conclusion, Se-MC has the potential to serve as a dietary supplement of selenium, an antioxidant, or an ingredient for the formulation of nutraceuticals. © 2014 American Chemical Society.

Wang J.-R.,South China Agricultural University | Wang J.-R.,Guangdong VTR Bio Technology Co. | Lin J.-F.,South China Agricultural University | Guo L.-Q.,South China Agricultural University | And 3 more authors.
World Journal of Microbiology and Biotechnology | Year: 2014

Squalene synthase (SQS) catalyzes the condensation of two molecules of farnesyl diphosphate to give presqualene diphosphate and the subsequent rearrangement to form squalene. The gene encoding squalene synthase was cloned from Poria cocos by degenerate PCR and inverse PCR. The open reading frame of the gene is 1,497 bp, which encodes 499 amino acid residues. A phylogenetic analysis revealed that P. cocos SQS belonged to the fungus group, and was more closely related to the SQS of Ganoderma lucidum than other fungi. The treatment of P. cocos with methyl jasmonate (MeJA) significantly enhanced the transcriptional level of P. cocos sqs gene and the content of squalene in P. cocos. The transcriptional level of sqs gene was approximately fourfold higher than the control sample and the squalene content reached 128.62 μg/g, when the concentration of MeJA was 300 μM after 72 h induction. © 2013 Springer Science+Business Media Dordrecht.

Liu L.,Hunan University | Liu L.,Fisheries Research Institute of Hunan Province | Li Y.L.,Hunan University | Xu S.D.,Guangdong VTR Bio Technology Co. | And 6 more authors.
Genetics and Molecular Research | Year: 2016

Myostatin (MSTN) is an important member of the transforming growth factor-β (TGF-β) superfamily and is a muscle growth inhibitor. In the present study, we cloned the Chinese perch MSTN cDNA sequence and analyzed its expression patterns under various conditions. The MSTN full cDNA sequence was 3347 bp long, including an open-reading frame of 1131 bp, which encoded 376 amino acids. Sequence analysis demonstrated that the MSTN shared a highly conserved signal peptide, a TGF-β functional peptide, a hydrolytic site (RARR), and nine conservative cysteine residues with other members of the TGF-β superfamily. Sequence alignment and phylogenetic tree analyses indicated that the MSTN had a close relationship with teleostean fish, but they are far separated from mammals. Real-time polymerase chain reaction analysis revealed that the MSTN was strongly expressed in the skeletal muscle and heart tissues. Temporal expression analysis demonstrated that the MSTN gene was expressed in very low levels, from 20 to 90 dph (post-hatching development), and was at its highest level at 150 dph (P < 0.05). The fasting-re-feeding experiment showed that the expression of the MSTN gene was initially decreased in response to a single meal, after seven days of fasting, and subsequently increased significantly, and finally decreased back to its original level. Together, our results provided valuable knowledge regarding the regulation of MSTN gene expression in Chinese perch. © FUNPEC-RP.

Xie D.,Shantou University | Xie D.,Henan Normal University | Xu S.,Shantou University | Xu S.,Guangdong VTR Bio Technology Co. | And 3 more authors.
Aquaculture | Year: 2016

Alpha-amylase (α-amylase) is a carbohydrase that plays a major role in carbohydrate metabolism. However, little is known about its molecular and biochemical characterisation in herbivorous marine teleosts. In the present study, an α-amylase gene was cloned and its tissue expression was determined in rabbitfish Siganus canaliculatus, a crucial cultured herbivorous marine teleost in China. The nutritional regulation of the gene, including its mRNA expression and enzymatic activity, was also investigated. The full length of its cDNA was 1903 bp, containing a 1539 bp open reading frame encoding a polypeptide of 512 amino acids, which possessed all the characteristic features of the α-amylase family. Its mRNA expression was detected in hepatopancreas, anterior intestine, middle intestine and posterior intestine, but not in other parts of the alimentary system. Four dietary groups of rabbitfish were each fed one of the following: raw fish (RF), formulated diet (FD), seaweed Enteromorpha prolifra (EP), or seaweed Gracilaria lemaneiformis (GL). After 8 weeks of feeding, the expression level of α-amylase mRNA in hepatopancreas, anterior intestine, middle intestine and posterior intestine showed no significant differences among the four dietary groups, whereas the α-amylase activity in the RF group was significantly lower than that in the other dietary groups (P< 0.001), and significantly higher in the mid and posterior intestine of fish fed GL than those in fish fed with EP (P< 0.01). These findings suggest that the nutritional regulation of α-amylase synthesis in rabbitfish may occur at the posttranscriptional level. This study is the first characterisation of the α-amylase gene from a herbivorous marine culture teleost. Statement of relevance: Alpha-amylase (α-amylase) is an important carbohydrase that plays a major role in carbohydrate metabolism. This study provides the first investigation of cloning and characterisation of α-amylase gene from a herbivorous marine-cultured teleost rabbitfish, and provides a foundation for the development of low-cost and effective formulated feed with seaweed as a dietary ingredient for rabbitfish. The work described has not been published or is under consideration for publication elsewhere. The study was reviewed and approved by the Ethics Committee of Animal Experiments of Shantou University. © 2015 Elsevier B.V.

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