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Qiu Y.,Guangdong Provincial Second Peoples Hospital
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2013

To investigate the changes in orbitofrontal cortex (OFC) functional connectivity and its association with decision-making deficits in chronic heroin-dependent individuals (HDIs) and explore the neural mechanisms of heroin addiction and relapse. Fourteen male chronic HDIs and 14 healthy subjects matched for age, education, and nicotine consumption participated in this study. Resting state functional magnetic resonance imaging (fMRI) was performed using a 1.5 T MR scanner. Functional connectivity of the OFC and the rest of the brain were calculated using REST software. Voxel-based analysis of the functional connectivity maps between the control and HDI groups was performed with two-sample t test. The Iowa gambling task (IGT) was used to assess the participants' decision making during uncertainty. Compared with the control group, the HDIs showed significantly decreased functional connectivity of the OFC and the right inferior parietal lobule (rIPL) (t=3.5, P<0.05). A significant negative correlation was noted between the functional connectivity of the OFC-rIPL and performance level at the IGT. The OFC-rIPL functional connectivity is significantly disrupted in HDIs, which may be the neural basis for decision-making deficits. Source


Wang H.,Guangdong Provincial Second Peoples Hospital | Luo S.,Guangdong Provincial Second Peoples Hospital
Journal of Burn Care and Research | Year: 2013

The aim of this study is to establish an animal model for human keloid scarring using tissue engineering method and to improve the research for keloid scarring in clinical and laboratory settings. After primary and passage culture, human keloid fibroblasts (KFBs) were transferred to poly(lactic-co-glycolic acid) (PLGA) copolymer and cultured in a rotatory cell culture system for 1 week. The complex of KFBs and PLGA (experimental group), and PLGA only (control group) were then transplanted to subcutaneous pouches in athymic mice. The implants were collected on days 30, 60, 120, and 180 for histological observation. All mice survived after surgery. The size of implants in the experimental group kept increasing from days 30 to 180, whereas the implants in control group became smaller. Using different histological stainings, KFB and collagen were observed at all time points in the implants under light microscopy. Large amounts of KFBs and collagen were found in the implants of day 180, which exhibited similar histological features to human keloid. Also, the fibroblasts in the implants had abundant rough endoplasmic reticulum in the cytoplasm under transmission electron microscopy. These findings indicate that the fibroblasts retain cellular characteristics in the implant. The combination of KFBs and PLGA can form keloid-like tissue in athymic mice. Establishment of this promising animal model for keloid is worthwhile, and this model might help our understanding of the pathological process and our ability to evaluate drug efficacy to human keloid scars in clinical trials. Copyright © 2012 by the American Burn Association. Source


Deng H.-H.,Guangdong Provincial Second Peoples Hospital | Ma S.-M.,Guangdong Provincial Second Peoples Hospital | Xiao X.-S.,Guangdong Provincial Second Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Dexmedetomidine is an effective high-selectivity α2-adrenoceptor agonist that has sedative, analgesic, anxiolytic effects, but slightly affects respiration. OBJECTIVE: To observe the analgesic effect of dexmedetomidine on selective damage of sciatic nerve branch in a rat model by intrathecal injection. METHODS: A total of 36 male Sprague-Dawley rats were randomly divided into normal control group, physiological saline group and dexmedetomidine group. A rat model of selective damage of sciatic nerve branch was established by knotting off the common peroneal nerve and tibial nerve in the physiological saline group and dexmedetomidine group. Rats in the dexmedetomidine group were daily injected with dexmedetomidine 3 μg/kg by intrathecal injection within 14 days after injury. Rats in the physiological saline group were injected with physiological saline. RESULTS AND CONCLUSION: Compared with the physiological saline group, the mechanical withdrawal threshold and the thermal withdrawal latency were significantly increased in the dexmedetomidine group (P < 0.05). Neuronal nitric oxide synthase mRNA and protein expression levels were significantly decreased in the spinal dorsal horn (P < 0.05). The injury to spinal dorsal horn neurons was obviously lessened. Moreover, neuronal nitric oxide synthase mRNA and protein expression levels and the injury to spinal dorsal horn neurons were similar between 14 days after administration and normal control group. Results indicated that intrathecal injection of dexmedetomidine could inhibit the expression of neuronal nitric oxide synthase in the spinal dorsal horn and relieve the pain induced by sciatic nerve injury. Source


Wei W.-J.,Guangdong Provincial Second Peoples Hospital | Xiao C.-J.,Guangdong Provincial Second Peoples Hospital | Li L.-H.,Guangdong Provincial Second Peoples Hospital | Jiang G.-H.,Guangdong Provincial Second Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: In order to avoid distal arterial embolism following mechanical thrombectomy, micro-balloon catheter temporary isolation is applied to prevent thrombus shedding. OBJECTIVE: To investigate the safety and feasibility of adopting the micro-balloon catheter technique in treatment of the hyperacute cerebral infarction. The micro-balloon catheter technique can temporarily block the artery blood flow and isolate the embolism location following mechanical thrombectomy and aspiration combined with thrombolysis. METHODS: Ten beagle dogs were included in this study. Under general anesthesia, the micro-balloon catheter was delivered to the dominant vertebral artery through the femoral artery in all the dogs and it was filled and temporarily blocked the blood flow. Then the autologous thrombus was injected through the micro-catheter into proximal vertebral artery to make a thrombosis model. All the dogs were equally divided into two groups according to the embolectomy method: control group (receiving pure stent embolectomy, n=5) and experimental group (n=5). The experiment group was disrupted and aspirated thrombus combined with the drug thrombolysis after temporarily blocking out the blood flow and isolating the target artery by micro-balloon catheter technique. After treatment, two groups underwent digital subtraction angiography to review the vertebral artery recanalization after different embolectomy methods. The hemodynamic status was evaluated through the thrombolysis in cerebral ischemia grade. All the dogs were scanned with magnetic resonance diffusion weighted imaging before modeling and at 12 hours after the thrombectomy. The animals were killed to perform pathological examination after magnetic resonance diffusion weighted imaging (12 hours after the thrombectomy). The vessel recanalization rates and complications were calculated in the two groups. RESULTS AND CONCLUSION: The thromboembolism model was successfully established in the dominant vertebral artery of all the 10 beagle dogs. In the control group, the vertebral arteries were completely successful recanalized in two dogs and were partly recanalized in three dogs, while the vertebral-basilar and intracranial arteries in one dog showed multiple small punctate filling defects with poor intracranial arterial development and contrast agent reflux. At 12 hours after embolectomy, the magnetic resonance diffusion weighted imaging showed slightly high signal intensity at the left temporoparietal lobe and the pathologic examination suggested thrombosis in the cerebral artery lumen of the left temporal lobe. In the experimental group, the vertebral arteries in five dogs were completely recanalized without infarction. The revascularization rate in the experimental group was significantly higher than that in the control group (P < 0.05). Experimental findings indicate that, the application of disruption and aspiration thrombus combined with the drug thrombolysis after temporarily blocking the blood flow and isolating the target artery by micro-balloon catheter technique in treatment of hyperacute cerebral infarction, can effectively prevent the small embolus exfoliating, which can cause distal embolization. Thus, the micro-balloon catheter technique is a safe, effective and relatively inexpensive interventional embolectomy. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source


Luo S.-K.,Guangdong Provincial Second Peoples Hospital | Chen G.-P.,Guangdong Provincial Second Peoples Hospital | Wang H.-B.,Guangdong Provincial Second Peoples Hospital | Sun Z.-S.,Guangdong Provincial Second Peoples Hospital | And 2 more authors.
Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery | Year: 2012

OBJECTIVE: To investigate the combined treatment with areola approach for capsular contracture after breast augmentation with implants.METHODS: From Feb. 2005 to Jun. 2011, 94 cases (168 sides) with Baker III and IV capsular contracture after breast augmentation with implants were treated with areola approach. The implants cavity was recreated, with or without removal of capsule. The implants were reimplanted behind pectoralis major or breast at the second stage in some patients.RESULTS: 46 cases were followed up by clinic visit and the others were followed up by telephone for 6-37 months, with an average of 9.9 months. The capsular contracture was relapsed in 2 cases as Baker III and 1 case as Baker IV. All the other breasts got a good appearance with good soft texture and feeling. No hematoma, infection, implants rupture, breast ptosis or implant displacement happened.CONCLUSIONS: Combined treatment with areola approach has a good therapeutic effect for capsular contracture after breast augmentation with implants. The breast appearance is satisfactory with low occurrence of capsular contracture. Source

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