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Ma S.,Qiqihar University | Liu X.,China Pharmaceutical University | Xu Q.,Heilongjiang Academy of Traditional Chinese Medicine | Zhang X.,Guangdong Research Institute of Traditional Chinese Medicine
Life Sciences | Year: 2014

In this report, the transport of ginkgolides with different lipophilicities was investigated using an hCMEC/D3 cell monolayer as a blood-brain barrier (BBB) cell model in vitro in an attempt to explain ginkgolide transport path mediated by lipophilicity.Main methods: The log P values of ginkgolides were determined by measuring the distribution of the molecule between oil and water. Additionally, the cytotoxicity of ginkgolides on hCMEC/D3 cells was assayed with the MTT method. Ginkgolide contents were determined with an ultra performance liquid chromatograph equipped with an evaporative light scattering detector (ULPC-ELSD) method. Apparent permeability coefficients (Papp) and efflux ratios (PappBL → AP/PappAP → BL) were then calculated to describe the transport characteristics of ginkgolide.Key findings: The transport of ginkgolide A, ginkgolide B, ginkgolide C, and ginkgolide J across the hCMEC/D3 cell monolayer was non-directional. Additionally, ginkgolide C transport on the cell monolayer was time- and concentration-dependent in the paracellular pathway controlled by cytochalasin D (a tight junction modulator). The transport of ginkgolide N, ginkgolide L, and ginkgolide K across the cell monolayer displayed clear directionality at low ginkgolide concentrations. This behavior indicated that the transport of ginkgolide N, ginkgolide L, and ginkgolide K was influenced by the transcellular pathway containing an efflux protein accompanied by the paracellular pathway for passive diffusion. Additionally, the transport of ginkgolide K was increased significantly by co-culturing with a P-gp inhibitor.Significance: These findings provide important information for elucidating ginkgolide transport pathways and may be beneficial for the design of ginkgolide molecules with high neuroprotective effects. © 2014 Elsevier Ltd. All rights reserved. Source


Wang W.-J.,Jinan University | Wu D.-L.,Jinan University | Liao S.-T.,Guangdong Academy of Agriculture science | Fan C.-L.,Jinan University | And 5 more authors.
Natural Product Communications | Year: 2013

Two new 2-phenylbenzofuran derivatives, atrofuran A (1) and atrofuran B (2), along with five known compounds, were isolated from the root bark of Morus atropurpurea. The structures of the new compounds were determined on the basis of extensive NMR spectroscopic and HR-ESI-MS analyses. Source


Mo X.L.,Guangdong Research Institute of Traditional Chinese Medicine
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To identify the different plant resources of Herba Rabdosiae Serrae by using Random Amplified Polymorphic DNA ( RAPD ) Analysis. The mini spin columns were used to extract the genomic DNA from five different plants of Herba Rabdosiae Serrae. With the DNA extracted from these plants as template,the 85 oligo nucleic acids (10 bp)as random primers,the polymer chain reaction (PCR) was done and the results were analysed by electro-pharoses. 12 primers were selected with polymorphism and 7 of them showed good polymorphism in RAPD map. RAPD method can be used to identify the plant resources of Herba Rabdosiae Serrae. Source


Zhang X.T.,Guangdong Research Institute of Traditional Chinese Medicine
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To study the pharmacokinetics of ginkgolide B for injection in rats. The serum concentration of ginkgolide B was determined by LC-MS and calculate its parameter of pharmacokinetics via DAS2.0 software. After intravenous of 0.75, 3.75 and 14.0 mg/kg ginkgolide B, parameters of pharmacokinetics of ginkgolide B were: Tmax were all (0.083 +/- 0) h, Cmax were (422.312 +/- 14.203), (1608.467 +/- 226.677), (1987.036 +/- 237.202) microg/L, AUC0-1 were (533.833 +/- 114.943), (1786.029 +/- 137.066), (1943.44 +/- 415.892) microg x h/L. Ginkgolide B has three compartment model in rats. Source


Zhang X.T.,Guangdong Research Institute of Traditional Chinese Medicine
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To study the pharmacokinetics of ginkgolide B injection in Beagle dogs. Determined the serum concentration of ginkgolide B by LC-MS and calculated its parameter of pharmacokinetics via DAS 2.0 software. After intravenous drips of 0.62, 2.07 and 10.35 mg/kg ginkgolide B, parameters of pharmacokinetics of ginkgolide B were as follows: Tmax were 0.444, 1, 1 h; Cmax were 0.764, 3.024, 11.013 mg/L; AUC(0-1) were 1.007, 3.644, 16.646 mg x h/Lo. Ginkgolide B has two compartment model in Beagle dogs. Source

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