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Chen W.,Guangdong Academy of Agricultural Sciences | Chen W.,The Key Laboratory of Animal Nutrition and Feed Science South China of Ministry of Agriculture | Chen W.,State Key Laboratory of Livestock and Poultry Breeding | Chen W.,Guangdong Public Laboratory of Animal Breeding and Nutrition | And 36 more authors.
Journal of Animal Science and Biotechnology | Year: 2016

Background: Although isoflavones are natural dietary antioxidants, they may have toxicological effects. This study aimed to evaluate the redox system in tissues of finishing pigs by supplementation with high dose of daidzein (640 mg/kg). Results: The supplementation of high dose of daidzein for 64 d increased the activity of superoxide dismutase and total antioxidant capacity in longissimus muscle but down-regulated the expression of reactive oxygen species (ROS)-producing enzyme NADPH oxidase-2 and cyclooxygenase-2. In contrast, high-level supplementation with daidzein exerted pro-oxidant changes in back fat, abdominal fat, liver, and plasma, as reflected by increased contents of malondialdehyde, a lipid peroxidation product, in these tissues. Furthermore, daidzein supplementation resulted in higher expression of ROS-producing enzymes, including NADPH oxidase-1 and cyclooxygenase-1 in liver, 5-lipoxygenase (5-LOX) in backfat and NADPH oxidase-2 both in abdominal fat and backfat. The supplementation of daidzein did not affect meat quality parameters in longissimus muscle, including marbling score, eye muscle areas, intramuscular fat, shear force, drip loss, pH and meat color. Conclusions: This experiment suggests that dietary supplementation of finishing pigs with daidzein at a high dose level improves redox status in muscle but exerts pro-oxidant effect in liver and fat tissues. © 2016 The Author(s).


Chen W.,Guangdong Academy of Agricultural Sciences | Chen W.,The Key Laboratory of Animal Nutrition and Feed Science South China of Ministry of Agriculture | Chen W.,State Key Laboratory of Livestock and Poultry Breeding | Chen W.,Guangdong Public Laboratory of Animal Breeding and Nutrition | And 21 more authors.
Food and Chemical Toxicology | Year: 2014

In order to explore the latter, the dose-response relationship of various concentrations of genistein on both cellular proliferation and the redox system were examined. The proliferation of primary muscle cells was promoted by a low concentration of genistein but was inhibited by high concentrations, which also enhanced lipid oxidation and suppressed membrane fluidity. By selecting a high concentration (200. μM) as a pro-oxidant treatment, the mechanism underlying the pro-oxidant function of genistein was then explored. The generation of intracellular reactive oxygen species (ROS) was stimulated by 200. μM genistein, with inhibited expression of NADPH oxidase 4 and cyclooxygenase 1 and 2 as well as increased activity of the glutathione redox system. The cellular expression of 5-lipoxygenase, however, was up-regulated by 200. μM genistein and the addition of 5-lipoxygenase inhibitor (Zileuton) decreased genistein-induced intracellular ROS level, close to that from the addition of the ROS scavenger, N-acetylcysteine. It is concluded that higher concentrations of genistein exert pro-oxidant potential in the primary muscle cells through enhancing ROS production in a 5-lipoxygenase-dependent manner. © 2014 Elsevier Ltd.


Ma X.,Guangdong Academy of Agricultural Sciences | Ma X.,The Key Laboratory of Animal Nutrition and Feed Science South China | Ma X.,State Key Laboratory of Livestock and Poultry Breeding | Ma X.,Guangdong Public Laboratory of Animal Breeding and Nutrition | And 26 more authors.
Genes and Nutrition | Year: 2016

Background: The mechanism of db-cAMP regulating fat deposition and improving lean percentage is unclear and needs to be further studied. Methods: Eighteen 100-day-old Duroc × Landrance × Large White barrows (49.75 ± 0.75 kg) were used for experiment 1, and 15 eighteen 135-day-old barrows (78.34 ± 1.22 kg) were used for experiment 2 to investigate the effects of dietary dibutyryl-cAMP (db-cAMP) on fat deposition in finishing pigs. Pigs were fed with a corn-soybean meal-based diet supplemented with 0 or 15 mg/kg db-cAMP, and both experiments lasted 35 days, respectively. Results: The results showed that db-cAMP decreased the backfat thickness, backfat percentage, and diameter of backfat cells without changing the growth performance or carcass characteristics in both experiments, and this effect was more marked in experiment 1 than in experiment 2; db-cAMP enhanced the activity of the growth hormone- insulin-like growth factor-1 (GH-IGF-1) axis and pro-opiomelanocortin (POMC) system in both experiments, which suppressed the accumulation of backfat deposition; microarray analysis showed that db-cAMP suppressed the inflammatory system within the adipose tissue related to insulin sensitivity, which also reduced fat synthesis. Conclusions: In summary, the effect of db-cAMP on suppressing fat synthesis and accumulation is better in the earlier phase than in the later phase of finishing pigs, and db-cAMP plays this function by increasing the activity of the GH-IGF-1 axis and POMC system, while decreasing the inflammatory system within the adipose tissue related to insulin sensitive or lipolysis. © 2016 The Author(s).


Ran X.-G.,Guangdong Academy of Agricultural Sciences | Ran X.-G.,State Key Laboratory of Livestock and Poultry Breeding | Ran X.-G.,The Key Laboratory of Animal Nutrition and Feed Science South China of Ministry of Agriculture | Ran X.-G.,Guangdong Public Laboratory of Animal Breeding and Nutrition | Wang L.-Y.,South China University of Technology
Journal of the Science of Food and Agriculture | Year: 2014

BACKGROUND: Relatively little attention is paid to collagen-rich cattle short tendons (musculus extensor communis, musculus flexor digitorum, musculus digitorum profundis) as a source of high content and relatively pure collagen, a meat-processing by-product that is used to a minimal extent. Thus, suitable extraction processes from a meat production by-product to gain intact collagen is promising, which thus become interesting from an economic and environmental point of view. RESULTS: Two extraction methods were compared: a 48 h pepsin treatment using 0.5 mol L-1 acetic acid and an extraction using pepsin treatment after ultrasonic treatment in a 0.5 mol L-1 acetic acid solution (the total ultrasonic and pepsin treatment time was 48 h). The results indicated that the optimal conditions for the extraction of collagen from cattle tendon with the ultrasonic-pepsin tandem method is: 4°C, tendon pre-swollen for 12 h in 0.5 mol L-1 acetic acid, pepsin amount: 50 U mg-1 of sample, ultrasonic-pepsin tandem treatment time for 18 h and 30 h, respectively. Extracted cattle tendon collagen using ultrasonic and pepsin treatment in tandem was characterised by amino acid analysis, SDS-PAGE, FT-IR, solubility and thermal denaturation temperature. The results show that the ultrasonic-pepsin tandem method can effectively improve the efficiency of pepsin extraction of natural collagen without any compromise of the resultant collagen quality. CONCLUSION: This study provides a favourable process to deal with poorly extractable residue by use of ultrasonic and pepsin treatment in tandem. Extracted collagen possesses an intact molecular structure, which is useful and particularly important for its biomedical applications, such as drug delivery systems, wound dressings, and scaffolds. © 2013 Society of Chemical Industry.


Yang X.F.,State Laboratory of Animal and Poultry Breeding | Yang X.F.,Key Laboratory of Animal Nutrition and Feed Science South China | Yang X.F.,Guangdong Public Laboratory of Animal Breeding and Nutrition | Yang X.F.,Guangdong Academy of Agricultural Sciences | And 28 more authors.
Canadian Journal of Animal Science | Year: 2016

This study tested the hypothesis that pre-weaning supplemental arginine may have a carry-over effect on intestinal growth and development of piglets immediately after weaning. Fifty-four [Duroc × (Landrace × Yorkshire)] piglets were fed a milk replacer diet supplemented with 0 (control), 4, or 8 g kg−1 of L-arginine from d 4 to 21 of age (6 replicate pens of 3 piglets per group). Piglets were then weaned to a common corn-soybean meal diet and fed for another 21 d. On day 42, 6 pigs per treatment were randomly selected for blood and tissue sampling. Arginine supplementation improved body weight of the piglets on d 42, average daily gain during d 22-31 (P < 0.05). Supplementation of 8 g kg−1 arginine decreased feed:gain (F:G) ratio in piglets during d 22-31 (P = 0.010). Compared with controls, 8 g kg−1 arginine improved villous height in duodenum, jejunum, and ileum; villous area in duodenum and jejunum; relative intestine weight; and plasma contents of insulin at d 42 (P < 0.05). Arginine supplementation increased mucosal protein content in all 3 segments of the small intestine (P < 0.05). These novel results clearly demonstrate a carry-over effect of pre-weaning supplementation with arginine on enhanced intestinal growth and development in the early post-weaning period. © 2016, Agricultural Institute of Canada. All rights reserved.


Chen Y.,Guangdong Academy of Agricultural Sciences | Chen Y.,South China Agricultural University | Chen Y.,Guangdong Public Laboratory of Animal Breeding and Nutrition | Cao J.-M.,Guangdong Academy of Agricultural Sciences | And 12 more authors.
Israeli Journal of Aquaculture - Bamidgeh | Year: 2014

An 8 week feeding trial was conducted to investigate the effects of dietary methionine levels on growth, feed utilization, body composition, and morphometric parameters of juvenile yellow catfish (Pelteobagrus fulvidraco), and to determine their dietary methionine requirement. Six isonitrogenous and isoenergetic diets (405.0 g/kg crude protein and 18.0 kJ/g gross energy) were formulated, and crystalline L-methionine was added to obtain dietary methionine levels of 5.5, 6.7, 7.5, 8.7, 9.5 and 11.3 g/kg dry diet. Fish with an initial weight of 1.4 g were randomly distributed into six groups and fed the respective formulated diets. Increasing amounts of dietary methionine up to 8.7 g/kg dry diet resulted in increased final body weight, rate of weight gain, specific growth rate, feed conversion efficiency and protein efficiency ratio however, a further increase in methionine level resulted in a decrease in these parameters. No significant difference in survival percentages was detected among the dietary treatments. A one-slope quadratic broken-line analysis model, based on specific growth rate and dietary methionine levels, indicated that the dietary L-methionine requirement of juvenile P. fulvidraco is 10.5 g/kg dry diet (accounting for 26.0 g/kg of dietary protein).


Ma X.,Guangdong Academy of Agricultural Sciences | Ma X.,Guangdong Public Laboratory of Animal Breeding and Nutrition | Lin Y.,Guangdong Academy of Agricultural Sciences | Lin Y.,Guangdong Public Laboratory of Animal Breeding and Nutrition | And 8 more authors.
Amino Acids | Year: 2010

The present study was conducted to test the hypothesis that dietary arginine supplementation may improve meat quality of finishing pigs. Beginning at ∼60 kg body weight, pigs were fed a corn- and soybean meal-based diet supplemented with 0, 0.5 or 1% l-arginine until they reached a body weight of ∼110 kg. On the last day of the experiment, pigs were food-deprived for 16 h before blood samples were obtained for analysis of amino acids, insulin, and other metabolites. Immediately thereafter, pigs were slaughtered for determination of carcass composition, muscle biochemical parameters, and meat quality. The result showed that arginine did not affect pig growth performance or carcass traits. However, 1% arginine decreased drip loss of pork muscle at 48 h postmortem, while increasing intramuscular fat content (P < 0.05). Supplementing 0.5 or 1% arginine to the diet increased arginine concentration and decreased cortisol level in serum, while enhancing antioxidative capacity and glutathione peroxidase activity in serum (P < 0.05). Additionally, 1% arginine increased antioxidative capacity in skeletal muscle (P < 0.05). Furthermore, 0.5 or 1% arginine decreased the cortisol receptor mRNA level in muscle (P < 0.05). Collectively, these results indicate that supplemental arginine improved meat quality and attenuated oxidative stress of finishing pigs. © 2008 Springer-Verlag.


Ma X.Y.,Guangdong Academy of Agricultural Sciences | Ma X.Y.,Guangdong Public Laboratory of Animal Breeding and Nutrition | Jiang Z.Y.,Guangdong Academy of Agricultural Sciences | Jiang Z.Y.,Guangdong Public Laboratory of Animal Breeding and Nutrition | And 4 more authors.
Journal of Animal Physiology and Animal Nutrition | Year: 2010

This study was conducted to determine the effect of dietary carnosine (β-alanyl-l-histidine) supplementation on antioxidant capacity and meat quality of pigs. 72 pigs approximately 60 kg were fed a corn- and soybean meal-based diet supplemented with 0, 25, 50 or 100 mg carnosine per kg diet for 8 weeks. Carnosine supplementation did not affect growth performance and carcass traits of pigs. However, the addition of 100 mg carnosine per kg diet increased pH value of muscle at 45 min, 24 h and 48 h postmortem. It also decreased drip loss at 48 h postmortem and increased redness value of muscle at 45 min postmortem (p < 0.05). The addition of 100 mg carnosine per kg diet enhanced glycogen concentration and Ca-ATPase activity at 24 and 48 h postmortem, and reduced malondialdehyde and carbonyl protein complexes concentrations in muscle at 24 h postmortem (p < 0.05). The addition of 100 mg carnosine per kg diet increased glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) activities in plasma, liver or muscle, as well as SOD and GSH-Px genes expression in muscle (p < 0.05). Taken together, these findings indicate that carnosine supplementation improves antioxidant capacity and meat quality of pigs. © 2010 Blackwell Verlag GmbH.

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