Guangdong Provincial Key Laboratory of Stomatology

Guangzhou, China

Guangdong Provincial Key Laboratory of Stomatology

Guangzhou, China
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Zhao K.,Sun Yat Sen University | Zhao K.,Guangdong Provincial Key Laboratory of Stomatology | Mai Q.-Q.,Peking University | Wang X.-D.,Sun Yat Sen University | And 2 more authors.
Journal of Dentistry | Year: 2013

Objectives To systematically review clinical outcomes of different occlusal designs of complete dentures. Methods Using a various key words, an electronic search of clinical trials published in English and Chinese literature was performed from four databases: Medline/PubMed, EMBASE, Cochrane Library, and CBM. Furthermore, a manual searching of the relevant journals and the bibliographies of reviews was performed. General satisfaction, masticatory ability, retention, and stability were major criteria for the evaluation of the outcomes. Studies met these criteria were selected for a full-text reading. The whole processes were performed by two reviewers independently. Results This systematic review started with 1030 articles, which were finally narrowed down to seven, according to the inclusion criteria. The following occlusal designs were included and analyzed: anatomic occlusion, balanced occlusion, canine guidance occlusion, lingualized occlusion, monoplane occlusion, and bilateral-balanced and canine-guided design. Three of the seven studies showed that lingualized occlusion had ratings of higher patients' satisfaction than other occlusion designs. On the other hand, the canine-guided occlusion dentures demonstrated equal or better clinical performances than bilateral-balanced dentures. Because of the heterogeneity and bias of the studies, it was not possible to analyze the data statistically. Conclusions Lingualized occlusion and canine-guided occlusion can be successfully applied in the fabrication of complete dentures. Canine guided occlusion has also been shown to be satisfactory. More well-controlled randomized trials are needed regarding canine-guided occlusion and the relationship between alveolar ridge resorption, different occlusal schemes and patient satisfaction. The conventional prosthodontic wisdom that complete dentures require a balanced occlusal design is not supported by the included literature. Clinical significance A suitable occlusal scheme would be a critical factor for a successful complete denture. However, there is no conclusive evidence to support which occlusal design is more appropriate for fabricating a successful complete denture. In occlusal design of conventional complete denture, lingualized occlusion can provide better clinical outcomes than other occlusal designs; meanwhile, canine-guided occlusion also can be used in clinic practice. © 2013 Elsevier Ltd.

Fan W.,Guangdong Provincial Key Laboratory of Stomatology | Fan W.,Sun Yat Sen University | Zhu X.,Guangdong Medical College | Wu L.,Guangdong Provincial Key Laboratory of Stomatology | And 4 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2015

Propofol is a short-acting intravenous anaesthetic agent and widely used not only in operating rooms but also in the intensive care unit (ICU). Apart from its multiple anaesthetic advantages, the neuroprotective effect of propofol has been demonstrated in diverse models of neuronal injury. The effect of propofol results from activation of gamma-aminobutyric acid (GABA) receptor, modulation excitatory amino acid transmitter system and protecting brain cells against oxidative stress. Moreover, propofol is able to supress apoptosis and inflammation and to regulate neuroprotection-associated proteins or ion homeostasis to act its neuroprotective effects. This review focuses on the research progress of the neuroprotective effects of propofol and its mechanisms of action to date. The implications for possible use for the clinical setting are also discussed.

PubMed | Guangdong Medical University, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat Sen University and Georgia Regents University
Type: | Journal: Life sciences | Year: 2016

Nitric oxide (NO) is a free radical gas in the biological system, which is produced by nitric oxide synthase (NOS) family. NO acts as a biological mediator and plays important roles in different systems in humans. The NO/NOS system exerts a broad spectrum of signaling functions involved in vasodilation, inflammation, oxidative stress, cardioprotection and neuroprotection. It has been demonstrated that intravenous and volatile anesthetics (such as propofol, ketamine, midazolam, isoflurane, sevoflurane, and desflurane, etc.) modulate NO production through multiple mechanisms that may influence physiological and pathophysiological processes. This review focuses on the effects of different anesthetics on NO/NOS regulation in different disease conditions. Possible cellular mechanisms and intermediate role of NO/NOS in anesthetic-mediated organ protection are also discussed. It would be interesting to clarify the impact of anesthetics on NO/NOS regulation. This review gives an overview of the effects of different anesthetics on NO/NOS regulation and function in different physiologic and pathophysiologic states.

Yang L.,Sun Yat Sen University | Yang L.,Guangdong Provincial Key Laboratory of Stomatology | Engeland C.G.,University of Illinois at Chicago | Cheng B.,Sun Yat Sen University | Cheng B.,Guangdong Provincial Key Laboratory of Stomatology
PLoS ONE | Year: 2013

Objective:To investigate the effects of social isolation on oral mucosal healing in rats, and to determine if wound-associated genes and microRNAs (miRNAs) may contribute to this response.Methods:Rats were group housed or socially isolated for 4 weeks before a 3.5 mm wound was placed on the hard oral palate. Wound closure was assessed daily and tissues were collected for determination of gene expression levels and miRNAs (i.e., miR-29a,b,c and miR-203). The predicted target of these microRNAs (i.e., vascular endothelial growth factor A, VEGFA) was functionally validated.Results:Social isolation stress delayed the healing process of oral palatal mucosal wounds in rats. Lower mRNA levels of interleukin-1β (IL1β), macrophage inflammatory protein-1α (MIP1α), fibroblast growth factor 7 (FGF7), and VEGFA were found in the biopsied tissues of isolated animals on days 1 and/or 3 post-wounding. Intriguingly, the isolated rats persistently exhibited higher levels of miR-29 family members and miR-203. Our results confirmed that VEGFA is a direct target of these miRNAs, as both miR-29a,c and miR-203 strongly and specifically suppressed endogenous VEGFA expression in vitro.Conclusions:This study in rats demonstrates for the first time that social isolation delays oral mucosal healing, and suggests a potential role for healing-associated gene and miRNA interactions during this process via modulation of VEGF expression. © 2013 Yang et al.

Chen Z.,Queensland University of Technology | Mao X.,Queensland University of Technology | Mao X.,Sun Yat Sen University | Mao X.,Guangdong Provincial Key Laboratory of Stomatology | And 6 more authors.
Biomaterials | Year: 2014

The osteoimmunomodulatory property of bone biomaterials is a vital property determining the in vivo fate of the implants. Endowing bone biomaterials with favorable osteoimmunomodulatory properties is of great importance in triggering desired immune response and thus supports the bone healing process. Magnesium (Mg) has been recognized as a revolutionary metal for applications in orthopedics due to it being biodegradable, biocompatible, and having osteoconductive properties. However, Mg's high rate of degradation leads to an excessive inflammatory response and this has restricted its application in bone tissue engineering. In this study, β-tricalcium phosphate (β-TCP) was used to coat Mg scaffolds in an effort to modulate the detrimental osteoimmunomodulatory properties of Mg scaffolds, due to the reported favorable osteoimmunomodulatory properties of β-TCP. It was noted that macrophages switched to the M2 extreme phenotype in response to the Mg-β-TCP scaffolds, which could be due to the inhibition of the toll like receptor (TLR) signaling pathway. VEGF and BMP2 were significantly upregulated in the macrophages exposed to Mg-β-TCP scaffolds, indicating pro-osteogenic properties of macrophages in β-TCP modified Mg scaffolds. This was further demonstrated by the macrophage-mediated osteogenic differentiation of bone marrow stromal cells (BMSCs). When BMSCs were stimulated by conditioned medium from macrophages cultured on Mg-β-TCP scaffolds, osteogenic differentiation of BMSCs was significantly enhanced; whereas osteoclastogenesis was inhibited, as indicated by the downregualtion of MCSF, TRAP and inhibition of the RANKL/RANK system. These findings suggest that β-TCP coating of Mg scaffolds can modulate the scaffold's osteoimmunomodulatory properties, shift the immune microenvironment towards one that favors osteogenesis over osteoclastogenesis. Endowing bone biomaterials with favorable osteoimmunomodulatory properties can be a highly valuable strategy for the development or modification of advanced bone biomaterials. © 2014 Elsevier Ltd.

Li X.,University of Sichuan | Ma Y.,Sun Yat Sen University | Ma Y.,Guangdong Provincial Key Laboratory of Stomatology | Wu X.,University of Sichuan | And 5 more authors.
Biochemical and Biophysical Research Communications | Year: 2013

Interleukin-6 (IL-6) is a potent stimulator of osteoclastic bone resorption. Osteocyte secretion of IL-6 plays an important role in bone metabolism. Serotonin (5-HT) has recently been reported to regulate bone metabolism. The aim of this study was to evaluate the effect of serotonin on osteocyte expression of IL-6. The requirement for the 5-HT receptor(s) and the role of the extracellular signal-regulated kinase 1/2 (ERK1/2) in serotonin-induced IL-6 synthesis were examined. In this study, real-time PCR and ELISA were used to analyse IL-6 gene and protein expression in serotonin-stimulated MLO-Y4 cells. ERK1/2 pathway activation was determined by Western blot. We found that serotonin significantly activated the ERK1/2 pathway and induced IL-6 mRNA expression and protein synthesis in cultured MLO-Y4 cells. However, these effects were abolished by pre-treatment of MLO-Y4 cells with a 5-HT2B receptor antagonist, RS127445 or the ERK1/2 inhibitor, PD98059. Our results indicate that serotonin stimulates osteocyte secretion of IL-6 and that this effect is associated with activation of 5-HT2B receptor and the ERK1/2 pathway. These findings provide support for a role of serotonin in bone metabolism by indicating serotonin regulates bone remodelling by mediating an inflammatory cytokine. © 2013 Elsevier Inc.

Liu J.,Sun Yat Sen University | Liu J.,Guangdong Provincial Key Laboratory of Stomatology | Huang F.,Sun Yat Sen University | Huang F.,Guangdong Provincial Key Laboratory of Stomatology | And 2 more authors.
International Journal of Molecular Sciences | Year: 2013

Melatonin is an endogenous hormone rhythmically produced in the pineal gland under the control of the suprachiasmatic nucleus (SCN) and the light/dark cycle. This indole plays an important role in many physiological processes including circadian entrainment, blood pressure regulation, seasonal reproduction, ovarian physiology, immune function, etc. Recently, the investigation and applications of melatonin in the hard tissues bone and tooth have received great attention. Melatonin has been investigated relative to bone remolding, osteoporosis, osseointegration of dental implants and dentine formation. In the present review, we discuss the large body of published evidence and review data of melatonin effects on hard tissues, specifically, bone and tooth. © 2013 by the authors; licensee MDPI, Basel, Switzerland.

Zhu W.,Sun Yat Sen University | Zhu W.,Guangdong Provincial Key Laboratory of Stomatology | Liang M.,Sun Yat Sen University | Liang M.,Guangdong Provincial Key Laboratory of Stomatology
Stem Cells International | Year: 2015

Periodontal ligament stem cells (PDLSCs), which reside in the perivascular space of the periodontium, possess characteristics of mesenchymal stem cells and are a promising tool for periodontal regeneration. Recently, great progress has been made in PDLSC transplantation. Investigators are attempting to maximize the proliferation and differentiation potential of PDLSCs by modifying culture conditions and applying growth factors. Nevertheless, problems remain. First, incomparability among different studies must be minimized by establishing standard guidelines for culture and identification of PDLSCs. Notably, attention should be paid to the biological safety of PDLSC transplantation. The present review updates the latest findings regarding PDLSCs and discusses standard criteria for culture and identification of PDLSCs. Finally, the review calls for careful consideration of PDLSC transplantation safety. © 2015 Wenjun Zhu and Min Liang.

Tao Y.,Sun Yat Sen University | Tao Y.,Guangdong Provincial Key Laboratory of Stomatology | Zhou Y.,Sun Yat Sen University | Zhou Y.,Guangdong Provincial Key Laboratory of Stomatology | And 4 more authors.
Archives of Oral Biology | Year: 2013

Objective: To monitor the longitudinal changes in oral microbial diversity of children with severe early childhood caries (S-ECC) compared to caries free (CF) controls. Methods: Dental plaque samples of 12 children in each group at 8, 14, 20, 26 and 32 months of age were analysed. Total microbial genomic DNA was isolated from each sample, and PCRdenaturing gradient gel electrophoresis (DGGE) analyses were carried out. Results: The number of bands was significantly higher in the CF group (18.17 ± 4.91 bands) than in the S-ECC group (14.54 ± 5.56 bands) at 32 months of age (P < 0.05). A total of 21 genera were identified in all subjects, and there were no significant differences between the two groups at genus level. DGGE profiles showed that most of the clusters were constructed from one individual over time in the both groups. Conclusions: The onset of S-ECC is accompanied by a decrease in microbial diversity. The overall composition of the microbiota is highly similar within an individual over time. © 2013 Elsevier Ltd. All rights reserved.

Liu J.,Sun Yat Sen University | Zhou H.,Sun Yat Sen University | Fan W.,Sun Yat Sen University | Fan W.,Guangdong Provincial Key Laboratory of Stomatology | And 6 more authors.
Journal of Pineal Research | Year: 2013

Melatonin mediates a variety of biological processes ranging from the control of circadian rhythms to immune regulation. Melatonin also influences bone formation and osteointegration of dental implants. However, the effects of melatonin on dentine formation have not been examined. This study investigated the effects of melatonin on the proliferation and differentiation of rat dental papilla cells (rDPCs) in vitro and dentine formation in vivo. We found that melatonin (0, 10-12, 10-10,10-8 m) induced a dose-dependent reduction in rDPCs proliferation, increased alkaline phosphatase (ALP) activity, the expression of dentine sialoprotein (DSP), and mineralized matrix formation in vitro. In vivo melatonin (50 mg/kg, BW, i.p.) inhibited dentine formation. Melatonin (10-8m) suppressed the activity of complex I and IV in the basal medium (OS-) and enhanced the activity of complex I and complex IV in osteogenic medium (OS+). These results demonstrate that melatonin suppresses the proliferation and promotes differentiation of rDPCs, the mechanisms of which may be related to activity of mitochondrial complex I and complex IV. © 2012 John Wiley & Sons A/S.

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