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Xue L.,Guangdong Institute of Microbiology | Xue L.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Xue L.,South China University of Technology | Wu Q.,Guangdong Institute of Microbiology | And 9 more authors.
Virus Genes | Year: 2013

The complete genome sequence of a novel norovirus strain GZ2010-L87 identified in Guangzhou was analyzed phylogenetically in this study. The RNA genome of the GZ2010-L87 strain is composed of 7,559 nucleotides. The phylogenetic analysis based on open reading frame (ORF) 2 revealed that the strain belongs to the GII.4 genotype, forming the new cluster GII.4-2009 which was also identified in Asia and the USA since 2009. Furthermore, phylogenetic analyses of the full genome and the different open reading frame sequences of GZ2010-L87 and other representative strains suggested that the novel strain did not undergo recombination. Comparative analysis with the consensus sequence of 31 completely sequenced norovirus GII.4-2009 genomes showed 86 mismatched nucleotides (56 in ORF1, 16 in ORF2, and 14 in ORF3), resulting in 19 amino acid changes (9 in ORF1, 3 in ORF2, and 7 in ORF3). Furthermore, 12 variable sites were found on the capsid protein of norovirus GII.4-2009, and most were located at the P2 domain. Meanwhile, based on comparison with other GII.4 clusters, 14 sites were shown specific to the novel cluster. In summary, the genome of the new GII.4-2009 variant GZ2010-L87, which was first identified in China, was extensively characterized with a large panel of genetically diverse noroviruses. The genomic information obtained from the novel variant can be used not only as a full-length norovirus sequence standard in China but also as reference data for future evolution research. © 2013 Springer Science+Business Media New York.

Deng D.,Guangdong Institute of Microbiology | Deng D.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Deng D.,Guangdong Open Laboratory of Applied Microbiology | Guo J.,Guangdong Institute of Microbiology | And 14 more authors.
International Biodeterioration and Biodegradation | Year: 2011

The environmental safety of decabromodiphenyl ether (deca-BDE) has been the topic of controversial discussions during the recent years. Reductive debromination of deca-BDE in the environment was proved to be a significant source of lower-brominated Polybrominated diphenyl ethers (PBDEs) to the ecosystem. Currently, very little is known about the susceptibility of deca-BDE to aerobic biotransformation. Lysinibacillus fusiformis strain DB-1, an aerobic bacterium capable of debromination of deca-BDE, was isolated from sediments of LianjiangRiver, Guiyu in Guangdong of China. DB-1 can efficiently transform deca-BDE to lower brominated BDEs using carbon sources such as lactate, pyruvate and acetate, respectively. In liquid cultures, free bromide concentration accumulated to 1220 μg L -1 with 6 mg L -1 of the nominal initial concentration of deca-BDE after 72 h aerobic incubation. The resting cell activity tests showed that debromination of deca-BDE by DB-1 was an aerobic process. This is the first report for biotransformation of deca-BDE by an indigenous bacterium isolated from PBDEs contaminated environment. © 2011 Elsevier Ltd.

Yang Y.,South China University of Technology | Yang Y.,Guangdong Institute of Microbiology | Yang Y.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Yang Y.,Guangdong Open Laboratory of Applied Microbiology | And 6 more authors.
Journal of Chemical Technology and Biotechnology | Year: 2011

OVERVIEW: Microbial fuel cells (MFCs) are an emerging technology which directly converts chemical energy stored in organic matter to electricity. Driven by the increasing concern over the energy-climate crisis and environment pollution, MFCs have been developed rapidly in the past decade. Currently, MFCs are making the challenging step from laboratory to practical application. This paper focuses on MFC patents and the applications of MFCs. IMPACT: MFCs make it possible to directly exploit bio-electricity from organic wastes with a higher energy transforming efficiency than other traditional technologies. The wide application of MFCs will significantly reduce the energy dependence on fossil fuel as well as the relative problems of climate and environmental pollution. APPLICATIONS: MFCs have been deployed in various practical environments, such as wastewater treatment plants, seafloor, etc. The electricity generated by MFCs has been used to charge low power devices. More applications have been funded or are to be undertaken. The successful pilot applications of MFCs promise a bright future for this technology. © 2011 Society of Chemical Industry.

Yang X.,Guangdong Institute of Microbiology | Yang X.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Wu Q.,Guangdong Institute of Microbiology | Wu Q.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | And 8 more authors.
PLoS ONE | Year: 2015

Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- is a monophasic variant of Salmonella Typhimurium, which has recently been recognized as an emerging cause of infection worldwide. This bacterium has also ranked among the four most frequent serovars causing human salmonellosis in China. However, there are no reports on its contamination in Chinese food. Serotyping, polymerase chain reaction, antibiotic resistance, virulotyping, and multilocus sequence typing (MLST) assays were used to investigate the prevalence of this serological variant in food products in China, and to determine phenotypic and genotypic difference of monophasic isolates and Salmonella Typhimurium isolated over the same period. Salmonella 1,4,[5],12:i:- was prevalent in various food sources, including beef, pork, chicken, and pigeon. The study also confirmed the high prevalence (53.8%) of resistance to ampicillin, streptomycin, sulfonamides, and tetracycline in Salmonella 1,4,[5],12:i:-, which was higher than that in Salmonella Typhimurium. Moreover, Salmonella 1,4,[5],12:i:- isolates in our study were different from Salmonella Typhimurium isolates by the absence of three plasmid-borne genes (spvC, pefA, and rck) and the presence of gipA in all isolates. All Salmonella 1,4,[5],12:i:- isolates demonstrated MLST pattern ST34. Genomic deletions within the fljBA operon and surrounding genes were only found in Salmonella 1,4,[5],12:i:- isolates, with all isolates containing a deletion of fljB. However, hin and iroB were identified in all Salmonella 1,4,[5],12:i:- isolates. Three different deletion profiles were observed and two of them were different from the reported Salmonella 1,4,[5],12:i:- clones from Spain, America, and Italy, which provided some new evidence on the independent evolution of the multiple successful monophasic clones from Salmonella Typhimurium ancestors. This study is the first report of Salmonella 1,4,[5],12:i:- in food products from China. The data are more comprehensive and representative, providing valuable information for epidemiological studies, risk management, and public health strategies. © 2015 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Chen X.,CAS South China Sea Institute of Oceanology | Chen X.,Guangdong Institute of Microbiology | Chen X.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Chen X.,University of Chinese Academy of Sciences | And 6 more authors.
Applied Microbiology and Biotechnology | Year: 2010

Electron transfer pathways for azoreduction by S. decolorationis S12 were studied using a mutant S12-22 which had a transposon insertion in ccmA. The results imply that there are two different pathways for electron transport to azo bonds. The colony of S12-22 was whitish and incapable of producing mature c-type cytochromes whose α-peak was at 553 nm in the wild type S12. The mutant S12-22 could not use formate as the sole electron donor for azoreduction either in vivo or in vitro, but intact cells of S12-22 were able to reduce azo dyes of low polarity, such as methyl red, when NADH was served as the sole electron donor. Although the highly polar-sulfonated amaranth could not be reduced by intact cells of S12-22, it could be efficiently reduced by cell extracts of the mutant when NADH was provided as the sole electron donor. These results suggest that the mature c-type cytochromes are essential electron mediators for the extracellular azoreduction of intact cells, while the other pathway without the involvement of mature c-type cytochromes, NADH-dependent oxidoreductase-mediated electron transfer pathway can reduce lowly polar sulfonated azo dyes inside the whole cells or highly polar sulfonated azo dyes in the cell extracts without bacterial membrane barriers. © 2009 Springer-Verlag.

Li W.-R.,Guangdong Institute of Microbiology | Li W.-R.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Xie X.-B.,Guangdong Institute of Microbiology | Xie X.-B.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | And 8 more authors.
Applied Microbiology and Biotechnology | Year: 2010

The antibacterial activity and acting mechanism of silver nanoparticles (SNPs) on Escherichia coli ATCC 8739 were investigated in this study by analyzing the growth, permeability, and morphology of the bacterial cells following treatment with SNPs. The experimental results indicated 10 μg/ml SNPs could completely inhibit the growth of 107 cfu/ml E. coli cells in liquid Mueller-Hinton medium. Meanwhile, SNPs resulted in the leakage of reducing sugars and proteins and induced the respiratory chain dehydrogenases into inactive state, suggesting that SNPs were able to destroy the permeability of the bacterial membranes. When the cells of E. coli were exposed to 50 μg/ml SNPs, many pits and gaps were observed in bacterial cells by transmission electron microscopy and scanning electron microscopy, and the cell membrane was fragmentary, indicating the bacterial cells were damaged severely. After being exposed to 10 μg/ml SNPs, the membrane vesicles were dissolved and dispersed, and their membrane components became disorganized and scattered from their original ordered and close arrangement based on TEM observation. In conclusion, the combined results suggested that SNPs may damage the structure of bacterial cell membrane and depress the activity of some membranous enzymes, which cause E. coli bacteria to die eventually. © 2009 Springer-Verlag.

Chen M.,South China University of Technology | Chen M.,Guangdong Institute of Microbiology | Chen M.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Wu Q.,Guangdong Institute of Microbiology | And 7 more authors.
Food Control | Year: 2014

Listeria monocytogenes is an important food-borne pathogen causing meningitis, meningoencephalitis and abortion. To assess the potential risk to consumer health, the presence of L.monocytogenes was investigated using qualitative and quantitative methods. Ten (6.33%) of 158 retail RTE food samples were positive for L.monocytogenes and the contamination levels were less than 10MPN/g, while none of 65 dairy products was positive for L.monocytogenes. The 37 strains were grouped into five clusters and two singletons, five clusters and two singletons, and three clusters and one singleton by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and RAPD fingerprint respectively, at similarity coefficient of 80%. The susceptibility test showed that 83.8% were susceptible to 15 antimicrobials; two were penicillin-resistant, and one was multidrug-resistant to kanamycin, tetracycline, sulfamethoxazole, rifampin, gentamycin, penicillin, and ampicillin. Virulent L.monocytogenes that possess partial antimicrobial resistance, and serotypes frequently associated with listeriosis were recovered from RTE foods. Consumers may, therefore, be exposed to potential risks of L.monocytogenes infection in South China. This study contributed to the prevalence and contamination levels of L.monocytogenes in RTE foods in South China for the first time, providing baseline information for Chinese regulatory authorities to formulate a regulatory framework for controlling L.monocytogenes to improve the microbiological safety of RTE foods.© 2013 Elsevier Ltd.

Sun L.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Sun L.,Guangdong Institute of Microbiology | Li D.-L.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Tao M.-H.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | And 2 more authors.
Helvetica Chimica Acta | Year: 2012

A new monocyclofarnesane-type sesquiterpene, 3,7,10-trihydroxy-6,11- cyclofarnes-1-ene (1), and a new acorane-type sesquiterpene, 8-(hydroxymethyl)-1-(2-hydroxy-1-methylethyl)-4-methylspiro[4.5]dec-8-en-7-ol (2), were isolated from the culture of Eutypella scoparia FS26 from the South China Sea, along with three known terpenes, 3-5. The structures of these compounds were determined by extensive analysis of their spectroscopic data as well as by comparison with literature reports. The isolated compounds 1-5 were evaluated for their cytotoxic activities against the SF-268, MCF-7, and NCI-H460 tumor cell lines. Copyright © 2012 Verlag Helvetica Chimica Acta AG, Zürich, Switzerland.

Ye Q.,South China University of Technology | Ye Q.,Guangdong Institute of Microbiology | Ye Q.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Wu Q.,Guangdong Institute of Microbiology | And 7 more authors.
Food Control | Year: 2016

Yersinia enterocolitica is an important food-borne enteropathogen that causes gastrointestinal syndromes. The aims of this study were to identify Y. enterocolitica in food samples in China, and to assess the pathogenic potential and antimicrobial resistance, and to characterize the genotypes of the isolates. From July 2011 to May 2014, a total of 2320 food samples were obtained, and 47 (2.03%) were found positive for Y. enterocolitica, while 706 retail-level ready-to-eat products and 249 vegetable samples were negative. A total of 58 Y. enterocolitica strains were isolated. All isolates belonged to biotype 1A, and the primary serotype was O:8. All strains lacked the ail, virF, ystA, and ystC virulence genes, but harbored the ystB, fepD, ymoA, fes, and sat genes. All 58 strains were sensitive to kanamycin and sulfonamide, but were resistant to two or more antibiotics. Most of the strains expressed the β-lactamase genes; the presence of blaA and blaB was detected in 97% and 100% of isolates, respectively. Many strains were resistant to trimethoprim/sulfamethoxazole (79.3%), ampicillin (91.4%), and cephalothin (91.4%). The 58 strains were grouped into three clusters and one singleton by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) at a similarity coefficient of 70%, and each cluster was largely organized by geographical region. This study provides a valuable accounting of the prevalence of Y. enterocolitica from a nationwide survey of foods in China, and highlights the seasonal effects of Y. enterocolitica prevalence in foods in China for the first time. © 2015 Elsevier Ltd.

PubMed | Guangdong Institute of Microbiology and Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application
Type: Journal Article | Journal: Journal of applied microbiology | Year: 2016

This study aims to develop a quick and sensitive method for obtaining GII.17 norovirus genome sequences based on a novel amplification strategy.Based on multiple alignments of GII.17 norovirus genome sequences available in GenBank, a set of primer pairs were first rationally designed, which could amplify six overlapping fragments encompassing the whole genome. Two sequencing primers II.17-Seq1R and II.17-Seq6F were also designed to complement sequences at both ends. The sensitivity of new primers was then evaluated by end-point dilution RT-PCR that was comparable to detection primers G2SKF/G2SKR. In practice, genome sequences of nine Guangzhou GII.17 strains were successfully obtained by the new method in one working day. All genomes comprised 7495 nucleotides with three complete ORFs, and their phylogenetic relationships were verified with other GII norovirus reference strains.Based on the new amplification strategy, a quick and sensitive method for direct sequencing of GII.17 norovirus genomes was successfully established.The newly developed method can be used as an important tool to collect genetic information of GII.17 noroviruses, and new obtained viral genomes in Guangzhou also provide reference data for norovirus research in future.

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