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Sun L.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Sun L.,Guangdong Institute of Microbiology | Li D.-L.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Tao M.-H.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | And 2 more authors.
Helvetica Chimica Acta | Year: 2012

A new monocyclofarnesane-type sesquiterpene, 3,7,10-trihydroxy-6,11- cyclofarnes-1-ene (1), and a new acorane-type sesquiterpene, 8-(hydroxymethyl)-1-(2-hydroxy-1-methylethyl)-4-methylspiro[4.5]dec-8-en-7-ol (2), were isolated from the culture of Eutypella scoparia FS26 from the South China Sea, along with three known terpenes, 3-5. The structures of these compounds were determined by extensive analysis of their spectroscopic data as well as by comparison with literature reports. The isolated compounds 1-5 were evaluated for their cytotoxic activities against the SF-268, MCF-7, and NCI-H460 tumor cell lines. Copyright © 2012 Verlag Helvetica Chimica Acta AG, Zürich, Switzerland.

Chen X.,Guangdong Institute of Microbiology | Chen X.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Chen X.,Guangdong Provincial Key Laboratory of Applied Microbiology | Sun G.,Guangdong Institute of Microbiology | And 5 more authors.
Journal of Applied Microbiology | Year: 2011

Aim: To investigate the role of soluble and insoluble iron in azoreduction by resting cells of Shewanella decolorationis S12.Methods and Results: A series of analytical experiments were carried out. Results showed that insoluble Fe 2O 3 all delayed the reduction of amaranth but did not inhibit it. Adsorption to Fe 2O 3 particles by the bacterial cell surface could be the reason leading to the delay in azoreduction. For the soluble iron, an important finding was that azoreduction activities were inhibited by soluble iron in high concentration because of its higher redox potential, and the inhibition was strengthened when the electron donor supply was insufficient. However, activities of azoreduction could be enhanced by low concentration of soluble iron. This stimulating effect was because of the electron transfer but not the cell growth.Conclusions: The effects of iron on azoreduction by the resting cells depended on the solubility and concentration of the iron compounds, which was different from what was observed by the growing cells in the previous studies.Significance and Impact of the Study: This study has both theoretical significance in the microbial physiology and practical significance in the bioremediation of azo dyes-contaminated environment. © 2010 The Authors. Journal of Applied Microbiology © 2010 The Society for Applied Microbiology.

Xue L.,Guangdong Institute of Microbiology | Xue L.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Xue L.,South China University of Technology | Wu Q.,Guangdong Institute of Microbiology | And 9 more authors.
Virus Genes | Year: 2013

The complete genome sequence of a novel norovirus strain GZ2010-L87 identified in Guangzhou was analyzed phylogenetically in this study. The RNA genome of the GZ2010-L87 strain is composed of 7,559 nucleotides. The phylogenetic analysis based on open reading frame (ORF) 2 revealed that the strain belongs to the GII.4 genotype, forming the new cluster GII.4-2009 which was also identified in Asia and the USA since 2009. Furthermore, phylogenetic analyses of the full genome and the different open reading frame sequences of GZ2010-L87 and other representative strains suggested that the novel strain did not undergo recombination. Comparative analysis with the consensus sequence of 31 completely sequenced norovirus GII.4-2009 genomes showed 86 mismatched nucleotides (56 in ORF1, 16 in ORF2, and 14 in ORF3), resulting in 19 amino acid changes (9 in ORF1, 3 in ORF2, and 7 in ORF3). Furthermore, 12 variable sites were found on the capsid protein of norovirus GII.4-2009, and most were located at the P2 domain. Meanwhile, based on comparison with other GII.4 clusters, 14 sites were shown specific to the novel cluster. In summary, the genome of the new GII.4-2009 variant GZ2010-L87, which was first identified in China, was extensively characterized with a large panel of genetically diverse noroviruses. The genomic information obtained from the novel variant can be used not only as a full-length norovirus sequence standard in China but also as reference data for future evolution research. © 2013 Springer Science+Business Media New York.

Yang Y.,South China University of Technology | Yang Y.,Guangdong Institute of Microbiology | Yang Y.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Yang Y.,Guangdong Open Laboratory of Applied Microbiology | And 6 more authors.
Journal of Chemical Technology and Biotechnology | Year: 2011

OVERVIEW: Microbial fuel cells (MFCs) are an emerging technology which directly converts chemical energy stored in organic matter to electricity. Driven by the increasing concern over the energy-climate crisis and environment pollution, MFCs have been developed rapidly in the past decade. Currently, MFCs are making the challenging step from laboratory to practical application. This paper focuses on MFC patents and the applications of MFCs. IMPACT: MFCs make it possible to directly exploit bio-electricity from organic wastes with a higher energy transforming efficiency than other traditional technologies. The wide application of MFCs will significantly reduce the energy dependence on fossil fuel as well as the relative problems of climate and environmental pollution. APPLICATIONS: MFCs have been deployed in various practical environments, such as wastewater treatment plants, seafloor, etc. The electricity generated by MFCs has been used to charge low power devices. More applications have been funded or are to be undertaken. The successful pilot applications of MFCs promise a bright future for this technology. © 2011 Society of Chemical Industry.

Ye Q.,South China University of Technology | Ye Q.,Guangdong Institute of Microbiology | Ye Q.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Wu Q.,Guangdong Institute of Microbiology | And 7 more authors.
Food Control | Year: 2016

Yersinia enterocolitica is an important food-borne enteropathogen that causes gastrointestinal syndromes. The aims of this study were to identify Y. enterocolitica in food samples in China, and to assess the pathogenic potential and antimicrobial resistance, and to characterize the genotypes of the isolates. From July 2011 to May 2014, a total of 2320 food samples were obtained, and 47 (2.03%) were found positive for Y. enterocolitica, while 706 retail-level ready-to-eat products and 249 vegetable samples were negative. A total of 58 Y. enterocolitica strains were isolated. All isolates belonged to biotype 1A, and the primary serotype was O:8. All strains lacked the ail, virF, ystA, and ystC virulence genes, but harbored the ystB, fepD, ymoA, fes, and sat genes. All 58 strains were sensitive to kanamycin and sulfonamide, but were resistant to two or more antibiotics. Most of the strains expressed the β-lactamase genes; the presence of blaA and blaB was detected in 97% and 100% of isolates, respectively. Many strains were resistant to trimethoprim/sulfamethoxazole (79.3%), ampicillin (91.4%), and cephalothin (91.4%). The 58 strains were grouped into three clusters and one singleton by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) at a similarity coefficient of 70%, and each cluster was largely organized by geographical region. This study provides a valuable accounting of the prevalence of Y. enterocolitica from a nationwide survey of foods in China, and highlights the seasonal effects of Y. enterocolitica prevalence in foods in China for the first time. © 2015 Elsevier Ltd.

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