Entity

Time filter

Source Type


Su M.,Chinese University of Hong Kong | Wu P.,Jinan University | Li Y.,Jinan University | Li Y.,Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug Research | Chung H.Y.,Chinese University of Hong Kong
Natural Product Communications | Year: 2010

Volatile oils from Centipeda minima extracted by steam distillation (SD) and supercritical fluid extraction (SFE) were investigated for their antiproliferative effects on the human nasopharyngeal cancer CNE cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay results showed that CNE cells were more susceptible to the SFE oil than to the SD oil. The IC 50 values of the SFE oil were 56.6, 8.7 and 5.2 μg/mL after 24-, 48- and 72-h of treatment, respectively, whereas those of the SD oil were 123.5 μg/mL (24 h), 97.1 μg/mL (48 h) and 83.3 μg/mL (72 h). Mechanistic investigation revealed that SFE oil induced CNE cell death via induction of apoptosis by regulating the expression of the Bcl-2 family of proteins, resulting in the dysfunction of mitochondria, leading to the release of cytochrome c into the cytosol, which then activated caspase-9, and subsequently cleaved caspases-3 and -7. This study provided strong evidence for the anti-NPC potential of the SFE oil from C. minima. Source


Wei Z.-C.,Jinan University | Yao Z.-H.,Jinan University | Yao Z.-H.,Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug Research | Wang Q.-Y.,Jinan University | And 6 more authors.
Chinese Traditional and Herbal Drugs | Year: 2015

To establish the UPLC fingerprint for effective quality control of Sarcandrae Herba. The fingerprint of Sarcandrae Herba was developed with ultra-performance liquid chromatography, and the Acquity UPLC HSS T3 column (50 mm × 2.1 mm, 1.8 μm) was used in the gradient elution with a mobile phase of acetonitrile-water (both containing 0.1% formic acid): The flow rate was 0.5 mL/min, the column temperature was 35℃, and the detection wavelength was at 330 nm. Similarity evaluation combined with hierarchical clustering analysis (HCA) and principal components analysis (PCA) were used to evaluate the quality of herbs from different areas. The fingerprint of Sarcandrae Herba was established with good precision, reproducibility, and stability obtaining within 23 min, and nine peaks in the fingerprint were designed. Eighteen samples could be classified into two clusters. The PCA result was consistent with the HCA. A brief list of five differential compounds is presented, including rosmarinic acid, newchlorogenic acid, and so on. The establishment of UPLC fingerprint of Sarcandrae Herba and the application of chemical pattern recognition can provide a more comprehensive reference for the quality control of herbs. ©, 2015, Chinese Traditional and Herbal Drugs. All right reserved. Source


Yao Z.-H.,Jinan University | Yao Z.-H.,Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug Research | Pan Y.-M.,Jinan University | Dai Y.,Jinan University | And 7 more authors.
Chinese Traditional and Herbal Drugs | Year: 2010

Objective To develop an HPLC characteristic chromatogram of Sarcandrae Herba water extract for evaluation of its quality and the related products. Methods The method was developed on a C18 column (250 mm×4. 6 mm, 5 μm) by gradient elution with acetonitrile-water (both containing 0. 1% formic acid) as mobile phase at a flow rate of 1. 0 mL/min with the detected wavelength at 330 nm and column temperature at 30 °C . The quality of the water extract and its related products were evaluated by comparing with the established reference characteristic chromatogram. Results The HPLC characteristic reference chromatogram was established with good stability, precision, and reproducibility. Its similarity among 12 batches of the extract and 20 batches of related preparations ranged in 0. 883 -0. 987 and 0. 797 -0. 982, respectively. Furthermore, eight peaks in the characteristic chromatogram were identified as neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, β-hydroxypropiovanillone, caffeic acid, isofraxidin, rosmarinic acid-4-O-glucoside, and rosmarinic acid, respectively. Conclusion With antiinflammatory bioactive ingredients and characteristic components included, the HPLC characteristic chromatogram revéales the profile of multiple chemical substances in Sarcandrae Herba water extract and its products and this would contribute to its effective quality control. Source


Zhang X.,Jinan University | Zhang X.,Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug Research | Wang G.,Jinan University | Wang G.,Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug Research | And 5 more authors.
Natural Product Communications | Year: 2011

Two new biflavonoids, 4′-methoxydaphnodorin D 1 and 4′-methoxydaphnodorin D 2, along with six known biflavonoids, were isolated from the roots of Wikstroemia indica. The structures of the new compounds were determined by extensive NMR and HRESIMS spectroscopic analyses in combination with CD measurements. Source


Hu Y.-M.,Jinan University | Hu Y.-M.,Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug Research | Wang G.-C.,Jinan University | Wang G.-C.,Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug Research | And 6 more authors.
Helvetica Chimica Acta | Year: 2012

Four C(8),C(12)-diastereoisomers, (8S,12S)-isoandrographolide (1), (8S,12R)-isoandrographolide (2), (8R,12R)-isoandrographolide (3), and (8R,12S)-isoandrographolide (4) were isolated from the aerial parts of Andrographis paniculata. The structures of the new compounds 1-3 were established on the basis of the spectroscopic data including UV, IR, NMR, HR-ESI-MS, and X-ray diffraction analysis. Copyright © 2012 Verlag Helvetica Chimica Acta AG, Zürich, Switzerland. Source

Discover hidden collaborations