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Yu D.,South China University of Technology | Yu S.-J.,South China University of Technology | Yu S.-J.,Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety
Food and Function | Year: 2016

Starch is a glucose polymer of vast importance to mankind. It forms the major component of all our staple foods. Starch is often used as an important material in cooking meat. In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared. The results showed that the addition of waxy rice starch and SCA significantly decreased PhIP, and the effect of SCA was more pronounced than that of waxy rice starch. This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate. This reaction path could disturb the reaction of creatinine with phenylacetaldehyde, subsequently influence the aldol condensation product formation, and finally suppress the formation of PhIP. Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction. So the effect of SCA was more pronounced than that of waxy rice starch on suppressing PhIP formation. A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system is also proposed. © The Royal Society of Chemistry 2016. Source


Guan Y.-G.,South China University of Technology | Yu P.,South China University of Technology | Yu S.-J.,South China University of Technology | Yu S.-J.,Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety | And 2 more authors.
Journal of Dairy Science | Year: 2012

A simultaneous analysis of reducing sugars and 5-hydroxymethyl-2-furaldehyde of the Maillard reaction products was detailed. It was based on a high performance anion exchange chromatography with electrochemical detector system and an HPLC with refractive index detector. Results showed that high performance anion exchange chromatography with electrochemical detector using a CarboPac PA-1 column (Dionex Corp., Sunnyvale, CA) was more suitable for reducing sugars and 5-hydroxymethyl-2-furaldehyde determination, especially for trace analysis. The lowest detectable limit of reducing sugars and 5-hydroxymethyl-2-furaldehyde was 0.00005. mol/L in this experiment. However, HPLC with a refractive index detector always produces a tailing peak for 5-hydroxymethyl-2-furaldehyde, and mannose and fructose cannot be absolutely separated. The results of the present study could provide a more sensitive means for 5-hydroxymethyl-2-furaldehyde and reducing sugar detection. © 2012 American Dairy Science Association. Source


Guo X.-M.,South China University of Technology | Zhu S.-M.,South China University of Technology | Tang Q.,South China University of Technology | Yu S.-J.,South China University of Technology | And 2 more authors.
Food Chemistry | Year: 2014

This paper was aimed at characterising the insoluble substances (IS) responsible for the turbidity of the extract and impurity of the resulting pectins. Results showed that the IS caused a significant increase in the turbidity of the extract. The IS had bi-pyramidal shapes under the SEM observation. The observed XRD peaks for the IS were similar to those of calcium oxalate dihydrate (COD). Moreover, the IS consisted of mainly oxalate and calcium. Results from the present study indicate the IS is COD. The influence of the IS on the purity of pectin was also studied. The presence of the IS in the pectins indicated the IS can precipitate with pectins during the alcohol precipitation, thereby contaminating the resulting pectins. © 2014 Elsevier Ltd. All rights reserved. Source


Wu X.-L.,South China University of Technology | Yu S.-J.,South China University of Technology | Yu S.-J.,Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety | Kang K.-R.,Guangzhou Wondfo Biotech Co.
Food Chemistry | Year: 2014

In this study, an indirect competitive enzyme-linked immunoassay (ic-ELISA) based on monoclonal antibody for 4(5)-Methylimidazole (4-MI) detection was described. The artificial antigens were prepared by conjugating bovine serum albumin (BSA) or ovalbumin (OVA) with the hapten of 4-MI. And monoclonal antibody, evaluated by ic-ELISA, was obtained by immunizing BABL/c mice. After optimizing, a standard curve for ic-ELISA detection on 4-MI was obtained with the linear detection range of 0.64-20.48 mg/L. The cross-reactivity (CR) of all the structural analogues of 4-MI was less than 5.62%. The recoveries of 4-MI in caramels detection were ranged from 88.69% to 114.09%, with relative standard deviation (n = 3) below 8.07%. The results suggested that the established ic-ELISA is promising for 4-MI commercial detection in caramels. © 2014 Elsevier Ltd. All rights reserved. Source


Xu X.-B.,South China University of Technology | Liu D.-B.,South China University of Technology | Yu S.-J.,South China University of Technology | Yu S.-J.,Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety | And 3 more authors.
Food Chemistry | Year: 2015

A method for simultaneous determination of 4-methylimidazole (4-MeI), 2-methylimidazole (2-MeI) and 5-hydroxymethylfurfural (HMF) in beverages was developed using solid-phase extraction (SPE) and amino trap column coupled with pulsed amperometric detection (AMTC-PAD). A single amino trap column (P/N: 046122) was first applied to separate the targeted analytes in samples after SPE pretreatment. This method demonstrated low limit of quantification (0.030 mg/L for methylimidazoles and 0.300 mg/L for HMF) and excellent linearity with correlation of determination (R2 = 0.999 for 2-MeI, 0.997 for 4-MeI and 0.998 for HMF). Nearly no 2-MeI was found in all soft drinks. However, 4-MeI could be detected in cola drinks and soft drinks containing caramel colour (ranging from 0.13 to 0.34 mg/L), whereas HMF were only found in cola drinks (ranging from 1.07 to 4.47 mg/L). Thus, AMTC-PAD technique would be a valid and inexpensive alternative to analysis of 4-MeI, 2-MeI and HMF. © 2014 Elsevier Ltd. All rights reserved. Source

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