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Chi Y.,South China Normal University | Yue Z.,Sun Yat Sen University | Liu Y.,Guangdong Province Hospital of Traditional Chinese Medicine | Mo L.,South China Normal University | Chen Q.,South China Normal University
Human Brain Mapping | Year: 2014

There are ongoing debates on whether object concepts are coded as supramodal identity-based or modality-specific representations in the human brain. In this fMRI study, we adopted a cross-modal "prime-neutral cue-target" semantic priming paradigm, in which the prime-target relationship was manipulated along both the identity and the modality dimensions. The prime and the target could refer to either the same or different semantic identities, and could be delivered via either the same or different sensory modalities. By calculating the main effects and interactions of this 2 (identity cue validity: "Identity_Cued" vs. "Identity_Uncued") × 2 (modality cue validity: "Modality_Cued" vs. "Modality_Uncued") factorial design, we aimed at dissociating three neural networks involved in creating novel identity-specific representations independent of sensory modality, in creating modality-specific representations independent of semantic identity, and in evaluating changes of an object along both the identity and the modality dimensions, respectively. Our results suggested that bilateral lateral occipital cortex was involved in creating a new supramodal semantic representation irrespective of the input modality, left dorsal premotor cortex, and left intraparietal sulcus were involved in creating a new modality-specific representation irrespective of its semantic identity, and bilateral superior temporal sulcus was involved in creating a representation when the identity and modality properties were both cued or both uncued. In addition, right inferior frontal gyrus showed enhanced neural activity only when both the identity and the modality of the target were new, indicating its functional role in novelty detection. © 2014 Wiley Periodicals, Inc.

Liang B.,University of South China | Zhang D.,Guangdong Province Hospital of Traditional Chinese Medicine | Zhang D.,Guangzhou University of Chinese Medicine | Wen X.,University of South China | And 5 more authors.
Frontiers in Human Neuroscience | Year: 2014

Previous studies have demonstrated that the difference between resting-state brain activations depends on whether the subject was eyes open (EO) or eyes closed (EC). However, whether the spontaneous fluctuations are directly related to these two different resting states are still largely unclear. In the present study, we acquired resting-state functional magnetic resonance imaging data from 24 healthy subjects (11 males, 20.17 ± 2.74 years) under the EO and EC states. The amplitude of the spontaneous brain activity in low-frequency band was subsequently investigated by using the metric of fractional amplitude of low frequency fluctuation (fALFF) for each subject under each state. A support vector machine (SVM) analysis was then applied to evaluate whether the category of resting states could be determined from the brain spontaneous fluctuations. We demonstrated that these two resting states could be decoded from the identified pattern of brain spontaneous fluctuations, predominantly based on fALFF in the sensorimotor module. Specifically, we observed prominent relationships between increased fALFF for EC and decreased fALFF for EO in sensorimotor regions. Overall, the present results indicate that a SVM performs well in the discrimination between the brain spontaneous fluctuations of distinct resting states and provide new insight into the neural substrate of the resting states during EC and EO. © 2014 Liang, Zhang, Wen, Xu, Peng, Huang, Liu and Huang.

Zhang N.,Shihezi University | Sun Z.-Z.,Peoples Hospital of the Xinjiang Uygur Autonomous Region | Fu X.-G.,Guangdong Province Hospital of Traditional Chinese Medicine
Chinese Journal of Pathology | Year: 2011

Objective To explore the relevance between the promoter methylation status of Notch1 gene and the invasive ductal carcinoma and ductal hyperplastic lesions of the breast. Methods Methylation status of Notch1 gene in human breast invasive ductal carcinoma ( IDC, n = 89 ) , ductal carcinoma in situ (DCIS, n=20) , atypical ductal hyperplasia (ADH, n = 11) and usual ductal hyperplasia (UDH, n=20) were quantitatively evaluated by MALDI-TOF MS. The expression of Notchl protein was detected by immunohistochemical stain (SP method). Results Positive expression rates of Notch1 protein in IDC and DCIS were 91. 0% (81/89) and 75. 0% ( 15/20) , respectively, which were significantly higher than those of ADH (4/11) and UDH (30.0%, 6/20; P < 0.05 ). Notch1 protein expression was correlated significantly with lymph node metastasis, pathological grades and TNM stages of IDC. The mean methylation levels of Notch1 gene at CpG-3, CpG-4. 5 and CpG-8 significantly decreased in IDC group compared with those of DCIS, ADH and UDH groups ( P < 0. 0083 ). In breast carcinomas, the mean methylation rates of Notchl gene at CpG-4. 5, CpG-10. 11 , and CpG-14. 15. 16 loci in cases with axillary node metastasis were significantly lower than those without axillary node metastasis (P < 0. 05 ) ; and the methylation rates at CpG-14. 15. 16 and CpG-18 loci in stage I were lower than that in stage II , further lower than that in stage III (P<0.05); and that in CpG-1.2, CpG-12. 13 loci in grade I (highly- differentiated group) were higher than that in grade II ( moderate-differentiated group ) and grade III ( poorly-differentiated group ) (P<0.05) ; and the methylation rates at CpG-3, CpG-8 and CpG-14. 15. 16 loci in ER* PR* HER2 - group were lower than that in ER - PR - HER2 + group ( P < 0. 05 ). Conclusions There is an overall hypomethylation of Notchl gene in breast invasive ductal carcinomas with corresponding over-expression of Notch1 protein. This inverse correlation show that the alteration of protein expression result from hypomethylation oncogene Notchl, and this change may have important significance in breast tumorigenesis and the development. Specific hypomethylation at CpG-3, CpG- 4. 5 and CpG-8 loci of Notchl gene may play a role in the pathogenesis of breast carcinoma, suggesting the progression and/or malignant transformation from benign glandular lesions of the breast.

Zhang D.,South China Normal University | Liu B.,Guangdong Province Hospital of Traditional Chinese Medicine | Chen J.,Guangdong Province Hospital of Traditional Chinese Medicine | Peng X.,South China Normal University | And 4 more authors.
PLoS ONE | Year: 2013

Recent studies have shown that multivariate pattern analysis (MVPA) can be useful for distinguishing brain disorders into categories. Such analyses can substantially enrich and facilitate clinical diagnoses. Using MPVA methods, whole brain functional networks, especially those derived using different frequency windows, can be applied to detect brain states. We constructed whole brain functional networks for groups of vascular dementia (VaD) patients and controls using resting state BOLD-fMRI (rsfMRI) data from three frequency bands - slow-5 (0.01~0.027 Hz), slow-4 (0.027~0.073 Hz), and whole-band (0.01~0.073 Hz). Then we used the support vector machine (SVM), a type of MVPA classifier, to determine the patterns of functional connectivity. Our results showed that the brain functional networks derived from rsfMRI data (19 VaD patients and 20 controls) in these three frequency bands appear to reflect neurobiological changes in VaD patients. Such differences could be used to differentiate the brain states of VaD patients from those of healthy individuals. We also found that the functional connectivity patterns of the human brain in the three frequency bands differed, as did their ability to differentiate brain states. Specifically, the ability of the functional connectivity pattern to differentiate VaD brains from healthy ones was more efficient in the slow-5 (0.01~0.027 Hz) band than in the other two frequency bands. Our findings suggest that the MVPA approach could be used to detect abnormalities in the functional connectivity of VaD patients in distinct frequency bands. Identifying such abnormalities may contribute to our understanding of the pathogenesis of VaD. © 2013 Zhang et al.

Yan T.-T.,Sun Yat Sen University | Li Q.,Sun Yat Sen University | Zhang X.-H.,Sun Yat Sen University | Wu W.-K.,Sun Yat Sen University | And 5 more authors.
Clinical and Experimental Pharmacology and Physiology | Year: 2010

1. Hyperhomocysteinaemia (HHcy) is associated with endothelial dysfunction and has been recognized as a risk factor of cardiovascular disease. The present study aimed to investigate the effect of homocysteine (Hcy) on endothelial function in vivo and in vitro, and the underlying signalling pathways. 2. The HHcy animal model was established by intragastric administration with l-methionine in rats. Plasma Hcy and nitric oxide (NO) concentration were measured by fluorescence immunoassay or nitrate reductase method, respectively. Vasorelaxation in response to acetylcholine and sodium nitroprusside were carried out on aortic rings. Human umbilical vein endothelial cells (HUVEC) were treated with indicated concentrations of Hcy in the in vitro experiments. Intracellular NO level and NO concentration in culture medium were assayed. The alterations of possible signalling proteins were detected by western blot analysis. 3. l-methionine administration induced a significant increase in plasma Hcy and decrease in plasma NO. Endothelium-dependent relaxation of aortic rings in response to acetylcholine was impaired in l-methionine-administrated rats. The in vitro study showed that Hcy reduced both intracellular and culture medium NO levels. Furthermore, Hcy decreased phosphorylation of endothelial nitric oxide synthase (eNOS) at serine-1177 and phosphorylation of Akt at serine-473. Hcy-induced dephosphorylation of eNOS at Ser-1177 was partially reversed by insulin (Akt activator) and GF109203X (PKC inhibitor). Furthermore, Hcy reduced vascular endothelial growth factor (VEGF) expression in a dose-dependent manner. 4. In conclusion, Hcy impaired endothelial function through compromised VEGF/Akt/endothelial nitric oxide synthase signalling. These findings will be beneficial for further understanding the role of Hcy in cardiovascular disease. © 2010 The Authors. Clinical and Experimental Pharmacology and Physiology © 2010 Blackwell Publishing Asia Pty Ltd.

Luo Y.,Institute of Acupuncture and Moxibustion | Xu N.-G.,Guangzhou University | Yi W.,Guangzhou University | Yu T.,Guangdong Province Hospital of Traditional Chinese Medicine | Yang Z.-H.,Guangzhou University
Chinese Journal of Integrative Medicine | Year: 2011

Objective: To observe the effects of electroacupuncture (EA) on the structure parameters of synapse and reactive changes of astrocyte in the marginal zone of focal cerebral ischemia in rats at different time zones so as to further explore its underlying mechanisms in the treatment of cerebral ischemia. Methods: Ninety male Wistar rats were randomly assigned to sham-operation, model, and EA groups, with 30 animals in each group. Each group was subdivided into 1 h, as well as 1, 3, 7, and 21 days post-operation groups, with 6 animals assigned to each time point subgroup. Heat coagulation-induced occlusion of the middle cerebral artery was performed to establish a model of focal cerebral ischemia. EA was applied immediately following surgery to the EA group [4/20 Hz, 2.0-3.0 V, 1-3 mA, to Baihui (GV20) and Dazhui (GV14)] for 30 min. Treatment was performed once a day, and experimental animals were sacrificed at 1 h, as well as 1, 3, 7 and 21 days postoperation. The ultrastructure changes in synapse and astrocytes were observed by using transmission electron microscopy. Glial fibrillary acidic protein (GFAP) expression and Ca2+ of astrocytes were measured by using laser confocal scanning microscope. Excitatory amino acid transporters-2 (EAAT2) and connexin 43 (CX43) expressions were assayed with immunohistochemical method. Canonical correlation analysis was conducted between structure parameters of synapse and parameters of astrocyte in the same time and group. Results: Broken synapses were observed following cerebral ischemia, and the numbers of synapses were significantly decreased. Compared with the model group, synaptic ultrastructure was significantly improved in the EA group. Compared with the sham-operation group, synaptic number density was significantly decreased, as were postsynaptic density thickness, synaptic cleft width and synaptic interface curvature in the EA and model groups. However, compared with the model group, postsynaptic density thickness was significantly increased in the EA group at the same time points post-operation (P<0.05, P<0.01). In addition, synaptic cleft width, synaptic number density and synaptic interface curvature were significantly increased with the passage of time (P<0.05, P<0.01). The expression of GFAP in the EA group were significantly lower than those in the model group at all the time points (P<0.05, P<0.01). OD values of EAAT2 in the EA group were significantly higher than those in the model group at the same time (P<0.05, P<0.01). Compared with that in the model group, the expressions of CX43 in the EA group increased significantly at 3 days and 7 days (P<0.05, P<0.01). Ca2+ average fluorescence intensity of astrocytes in the EA group was significantly lower than those in the model group at 1 h, 1 day, 3 days and 7 days (P<0.05, P<0.01). The changes in structure parameters of synapse were closely related to the changes of CX43, EAAT2, GFAP, Ca2+ of astrocytes by EA treatment at all the time points. Conclusions: EA is helpful for synaptic reorganization, which may be related to its effect on intervening the activation state of astrocytes and promoting the beneficial interaction between astrocytes and synapses. Acupuncture could start the adjustment of neuron-glial network so as to promote the synaptic reorganization, which may be the key mechanism of treating cerebral ischemia. © The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag Berlin Heidelberg 2011.

Yang Z.M.,Guangdong Province Hospital of Traditional Chinese Medicine
IET Conference Publications | Year: 2012

Objective: To study an effective solution for the information management and automatic statistical analysis of population of sub-health state. Method: a systematic analysis of business operations processes and modules division, designed to achieve system function analysis, database design, analysis, and network topology, through the actual use of specific investigations to verify the effectiveness and practicality of the system. Results: The system realizes some targets, which includes the survey data collection, storage, analysis, and network sharing, and also accesses to physical medicine, psychology, social coping, lifestyle, quality of life of the distribution to the sub-health status of those surveyed population, and achieves individual subjects personalized recommendations nursed back to health, and provides a basis and information platform for the sub-health state intervention. Conclusion: The system provides a good data management and analysis platform for sub-health population surveys, management and intervention.

Zhou H.,Sun Yat Sen University | Zhou H.,Guangdong Medical College | Liu Z.,Sun Yat Sen University | Liu J.,Sun Yat Sen University | And 7 more authors.
American Journal of Neuroradiology | Year: 2011

BACKGROUND AND PURPOSE: Radiation-induced cognitive dysfunction is a common and serious complication after radiation therapy of brain tumor, yet knowledge of its mechanism is poorly understood. The aim of this study was to establish a young rat model for acute radiation encephalopathy, at both cognitive and pathologic levels, induced by fractionated irradiation. MATERIALS AND METHODS: Four-week-old male rats were randomized into sham (0 Gy) and 2 experimental groups receiving fractionated irradiation of 5 Gy/day, 5 days/week, with total doses of 20 and 40 Gy, respectively. Cognition, BBB integrity, and potential astrogliosis were evaluated at 0, 4, 8, and 12 weeks' postirradiation. RESULTS: Twenty-Gy irradiation led to transient cognitive impairment only at 4 weeks' postirradiation. Forty-Gy irradiation induced cognitive impairment at both 4 and 8 weeks' postirradiation, which was more severe than that induced by 20 Gy. Cognitive impairment was accompanied by a transient increase in BWC only at 4 weeks for the 40-Gy group. Disrupted BBB permeability was detected at 4 and 8 weeks' postirradiation for the 20-Gy group, and at 4, 8, and 12 weeks' postirradiation for 40-Gy group, respectively. Increased astrogliosis in the hippocampus could be detected at 4 weeks' postirradiation for 40-Gy group. CONCLUSIONS: Fractionated irradiation in this experiment could induce acute brain injury, leading to cognitive impairment in young rats. BBB disruption might be a sensitive index for acute radiation encephalopathy. In addition, reactive astrogliosis might play an important role in this process. The present model, especially the 40-Gy irradiation group, is useful for basic and therapeutic studies of acute radiation encephalopathy.

PubMed | Yangtze University, Meharry Medical College, Guangdong Province Hospital of Traditional Chinese Medicine and Wuhan University
Type: Journal Article | Journal: Annals of clinical and laboratory science | Year: 2016

This study was to examine the inhibitory effect of matrine on the proliferation and metastasis of gastric cancer cells, and to explore the possible mechanisms involved in these processes. MTT was used to evaluate the proliferation ability of SGC7901 cells. A two and three-dimensional cell migration assay were performed to determine the effect of matrine on the migration of SGC7901 cells. Then, the changes of the uPA protein and other possible signal molecules were detected by western blot. We found that the proliferation ability of SGC 7901 cells was suppressed by matrine (p<0.05), and the average cell migration velocity was also significantly inhibited by matrine when compared to the control in a two-dimensional cell migration assay. In addition, SGC7901cells treated with matrine (50g/ml) migrated less than the control cells in a three-dimensional cell migration assay. At the meantime, the decreased uPA protein expression in SGC7901 cells treated with matrine was observed, and the PI3K/Akt pathway was inhibited. These results suggested that matrine can inhibit the proliferation and metastasis of gastric cancer cells through the PI3K/Akt/uPA pathway, indicating that matrine might be a potential molecular target for treatment of gastric carcinoma.

PubMed | Guangdong Province Hospital of Traditional Chinese Medicine, Guangzhou Medical College, Henan Provincial Peoples Hospital, Shandong University and 7 more.
Type: Journal Article | Journal: AJNR. American journal of neuroradiology | Year: 2016

On the basis of the high 1-month stroke and/or death (14.7%) rates associated with stent placement in the Stenting versus Aggressive Medical Management for Preventing Recurrent Stroke in Intracranial Stenosis trial, modifications in patient selection and procedural aspects for intracranial stent placement have been recommended. We performed a multicenter prospective single-arm trial to determine whether such modifications would result in lower rates of periprocedural stroke and/or death.The study enrolled patients with recent transient ischemic attack or ischemic stroke (excluding perforator ischemic events) related to high-grade (70%-99% in severity) stenosis of a major intracranial artery. Patients were treated by using angioplasty and self-expanding stents 3 weeks after the index ischemic event at 1 of the 10 high-volume centers in China. An independent neurologist ascertained the occurrence of any stroke and/or death within 1 month after the procedure.A total of 100 consecutive patients were recruited. The target lesions were located in the middle cerebral artery (M1) (n = 38, 38%), intracranial internal carotid artery (n = 17, 17%), intradural vertebral artery (n = 18, 18%), and basilar artery (n = 27, 27%). The technical success rate of stent deployment with residual stenosis of <50% was 100%. The overall 1-month stroke and/or death rate was 2% (95% confidence interval, 0.2%-7.0%). Two ischemic strokes occurred in the pontine region (perforator distribution) in patients following angioplasty and stent placement for basilar artery stenosis.The results of this prospective multicenter study demonstrated that modifications in patient selection and procedural aspects can substantially reduce the 1-month stroke and/or death rate following intracranial stent placement.

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