Guangdong Open Laboratory of Applied Microbiology

Guangzhou, China

Guangdong Open Laboratory of Applied Microbiology

Guangzhou, China
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Pan Y.,Guangdong Institute of Microbiology | Pan Y.,South China University of Technology | Pan Y.,State Key Laboratory of Applied Microbiology Southern China | Pan Y.,Guangdong Open Laboratory of Applied Microbiology | And 9 more authors.
Frontiers in Microbiology | Year: 2017

Microbial iron reduction is an important biogeochemical process and involved in various engineered processes, including the traditional clay dyeing processes. Bioaugmentation with iron reducing bacteria (IRB) is generally considered as an effective method to enhance the activity of iron reduction. However, limited information is available about the role of IRB on bioaugmentation. To reveal the roles of introduced IRB on bioaugmentation, an IRB consortium enriched with ferric citrate was inoculated into three Fe(II)-poor sediments which served as the pigments for Gambiered Guangdong silk dyeing. After bioaugmentation, the dyeabilities of all sediments met the demands of Gambiered Guangdong silk through increasing the concentration of key agent [precipitated Fe(II)] by 35, 27, and 61%, respectively. The microbial community analysis revealed that it was the minor species but not the dominant ones in the IRB consortium that promoted the activity of iron reduction. Meanwhile, some indigenous bacteria with the potential of iron reduction, such as Clostridium, Anaeromyxobacter, Bacillus, Pseudomonas, Geothrix, and Acinetobacter, were also stimulated to form mutualistic interaction with introduced consortium. Interestingly, the same initial IRB consortium led to the different community successions among the three sediments and there was even no common genus increasing or decreasing synchronously among the potential IRB of all bioaugmented sediments. The Mantel and canonical correspondence analysis showed that different physiochemical properties of sediments influenced the microbial community structures. This study not only provides a novel bioremediation method for obtaining usable sediments for dyeing Gambiered Guangdong silk, but also contributes to understanding the microbial response to IRB bioaugmentation. © 2017 Pan, Yang, Xu and Sun.


Yang Y.,Guangdong Institute of Microbiology | Kong G.,State Key Laboratory of Applied Microbiology Southern China | Chen X.,Guangdong Institute of Microbiology | Lian Y.,Guangdong Institute of Microbiology | And 3 more authors.
Frontiers in Microbiology | Year: 2017

Bacterial extracellular electron transfer (EET) plays a key role in various natural and engineering processes. Outer membrane c-type cytochromes (OMCs) are considered to be essential in bacterial EET. However, most bacteria do not have OMCs but have redox proteins other than OMCs in their extracellular polymeric substances of biofilms. We hypothesized that these extracellular non-cytochrome c proteins (ENCP) could contribute to EET, especially with the facilitation of electron mediators. This study compared the electrode respiring capacity of wild type Shewanella decolorationis S12 and an OMC-deficient mutant. Although the OMC-deficient mutant was incapable in direct electricity generation in normal cultivation, it regained electricity generation capacity (26% of the wide type) with the aid of extracellular electron mediator (riboflavin). Further bioelectrochemistry and X-ray photoelectron spectroscopy analysis suggested that the ENCP, such as proteins with Fe-S cluster, may participate in the falvin-mediated EET. The results highlighted an important and direct role of the ENCP, generated by either electricigens or other microbes, in natural microbial EET process with the facilitation of electron mediators. © 2017 Yang, Kong, Chen, Lian, Liu and Xu.


Li J.,South China University of Technology | Li J.,Guangdong Institute of Microbiology | Li J.,Guangdong Open Laboratory of Applied Microbiology | Li J.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | And 10 more authors.
Applied Microbiology and Biotechnology | Year: 2012

Two identical biotrickling filters named BTFa and BTFb were run in parallel to examine their performances in removing hydrogen sulfide. BTFa was filled with ceramic granules, and BTFb was filled with volcanic rocks. The results showed that BTFb was more robust than BTFa under acidic conditions. At empty bed residence times (EBRTs) of 20 and 15 s, the removal efficiency of BTFa was close to 100%. At EBRTs of 10 and 5 s, the removal efficiency of BTFa slightly decreased. The removal efficiencies of BTFa decreased by different degrees at the end of each stage, dropping to 94%, 81%, 60%, and 71%, respectively. However, the H2S removal efficiency in BTFb consistently reached 99% throughout the experiment. Pyrosequencing analyses indicated that members of Thiomonas dominated in both BTFs, but the relative abundance of Acidithiobacillus was higher in BTFb than in BTFa. © 2011 Springer-Verlag.


Yang Y.,Guangdong Institute of Microbiology | Yang Y.,Guangdong Academy of science | Guo J.,Guangdong Academy of science | Sun G.,Guangdong Institute of Microbiology | And 5 more authors.
Bioresource Technology | Year: 2013

Microbial electrochemical snorkel (MES) reactor is a simplified bioreactor based on microbial fuel cells (MFCs) and has been suggested to be a promising approach to solve many environmental problems. However, the microbial processes in MES reactors have not yet been characterized. This study shows that Shewanella decolorationis S12 can use the conductive snorkel as direct electron acceptor for respiration and growth. Similar with current-generating biofilms, cellular viability in MES biofilms decreased with the distance from snorkel. MES reactors showed more rapid cell growth and substrate consumption than MFCs. Although the biomass density of MES biofilm was higher than that of anode biofilms, the current-generating capacity and electrochemical activity of MES biofilm were lower, which could be attributed to the lower cytochrome c expression in MES biofilm caused by the higher redox potential of MES. These microbiological and electrochemical properties are essential for the further development of MES reactors. © 2012 Elsevier Ltd.


Yang Y.,Guangdong Institute of Microbiology | Yang Y.,State Key Laboratory of Applied Microbiology Ministry Guangdong Province Jointly Breeding Base | Yang Y.,Guangdong Open Laboratory of Applied Microbiology | Xu M.,Guangdong Institute of Microbiology | And 8 more authors.
Process Biochemistry | Year: 2012

Bioelectrochemical systems (BES), typically microbial fuel cells (MFCs), have attracted increasing attention in the past decade due to their promising applications in many fields, such as bioremediation, energy generation and biosynthesis. Current-generating microorganisms play a key role in BES. The process of transferring electrons to electrode has been considered as a novel anaerobic bacteria respiration, and more and more bacteria capable of exchanging electrons with electrodes have been isolated. Among those bacteria, Shewanella and Geobacter genera are the most frequently used model organisms in the studies of BES, as well as the bacteria-electrode electron transfer mechanisms. Many significant new findings in the field of the bacterial extracellular electron transfer in BES have been reported recently. A better understanding of the mechanisms of bacterial extracellular electron transfer would provide more efficient strategies to enhance the applicability of BES. This review summarizes the recent advances of extracellular electron transfer mechanisms with foci on Shewanella and Geobacter species in BES. © 2012 Elsevier Ltd.


Xu M.,Guangdong Institute of Microbiology | Xu M.,State Key Laboratory of Applied Microbiology | Xu M.,Guangdong Open Laboratory of Applied Microbiology | Chen X.,Guangdong Institute of Microbiology | And 15 more authors.
PLoS ONE | Year: 2012

Polybrominated diphenyl ethers (PBDEs) can be reductively degraded by microorganisms under anaerobic conditions. However, little is known about the effect of electron donors on microbial communities involved in PBDEs degradation. Here we employed 454 Titanium pyrosequencing to examine the phylogenetic diversity, composition, structure and dynamics of microbial communities from microcosms under the conditions of different electron donor amendments. The community structures in each of the five alternate electron donor enrichments were significantly shifted in comparison with those of the control microcosm. Commonly existing OTUs between the treatment and control consortia increased from 5 to 17 and more than 50% of OTUs increased around 13.7 to 186 times at least in one of the microcosms after 90-days enrichment. Although the microbial communities at different taxonomic levels were significantly changed by different environmental variable groups in redundancy analysis, significant correlations were observed between the microbial communities and PBDE congener profiles. The lesser-brominated PBDE congeners, tri-BDE congener (BDE-32) and hexa-BDE, were identified as the key factors shaping the microbial community structures at OTU level. Some rare populations, including the known dechlorinating bacterium, Dehalobacter, showed significant positive-correlation with the amounts of PBDE congeners in the consortia. The same results were also observed on some unclassified bacteria. These results suggest that PBDEs-degrading microbial communities can be successfully enriched, and their structures and compositions can be manipulated through adjusting the environmental parameters. © 2012 Xu et al.


Pan T.,Guangdong Institute of Microbiology | Pan T.,South China University of Technology | Pan T.,Guangdong Open Laboratory of Applied Microbiology | Pan T.,State Key Laboratory of Applied Microbiology | And 12 more authors.
Applied Microbiology and Biotechnology | Year: 2013

The biological treatment of triphenylmethane dyes is an important issue. Most microbes have limited practical application because they cannot completely detoxicate these dyes. In this study, the extractive biodecolorization of triphenylmethane dyes by Aeromonas hydrophila DN322p was carried out by introducing the cloud point system. The cloud point system is composed of a mixture of nonionic surfactants (20 g/L) Brij 30 and Tergitol TMN-3 in equal proportions. After the decolorization of crystal violet, a higher wet cell weight was obtained in the cloud point system than that of the control system. Based on the results of thin-layer chromatography, the residual crystal violet and its decolorized product, leuco crystal violet, preferred to partition into the coacervate phase. Therefore, the detoxification of the dilute phase was achieved, which indicated that the dilute phase could be discharged without causing dye pollution. The extractive biodecolorization of three other triphenylmethane dyes was also examined in this system. The decolorization of malachite green and brilliant green was similar to that of crystal violet. Only ethyl violet achieved a poor decolorization rate because DN322p decolorized it via adsorption but did not convert it into its leuco form. This study provides potential application of biological treatment in triphenylmethane dye wastewater. © 2012 Springer-Verlag Berlin Heidelberg.


Deng D.,Guangdong Institute of Microbiology | Deng D.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Deng D.,Guangdong Open Laboratory of Applied Microbiology | Guo J.,Guangdong Institute of Microbiology | And 14 more authors.
International Biodeterioration and Biodegradation | Year: 2011

The environmental safety of decabromodiphenyl ether (deca-BDE) has been the topic of controversial discussions during the recent years. Reductive debromination of deca-BDE in the environment was proved to be a significant source of lower-brominated Polybrominated diphenyl ethers (PBDEs) to the ecosystem. Currently, very little is known about the susceptibility of deca-BDE to aerobic biotransformation. Lysinibacillus fusiformis strain DB-1, an aerobic bacterium capable of debromination of deca-BDE, was isolated from sediments of LianjiangRiver, Guiyu in Guangdong of China. DB-1 can efficiently transform deca-BDE to lower brominated BDEs using carbon sources such as lactate, pyruvate and acetate, respectively. In liquid cultures, free bromide concentration accumulated to 1220 μg L -1 with 6 mg L -1 of the nominal initial concentration of deca-BDE after 72 h aerobic incubation. The resting cell activity tests showed that debromination of deca-BDE by DB-1 was an aerobic process. This is the first report for biotransformation of deca-BDE by an indigenous bacterium isolated from PBDEs contaminated environment. © 2011 Elsevier Ltd.


Yang Y.,South China University of Technology | Yang Y.,Guangdong Institute of Microbiology | Yang Y.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Yang Y.,Guangdong Open Laboratory of Applied Microbiology | And 6 more authors.
Journal of Chemical Technology and Biotechnology | Year: 2011

OVERVIEW: Microbial fuel cells (MFCs) are an emerging technology which directly converts chemical energy stored in organic matter to electricity. Driven by the increasing concern over the energy-climate crisis and environment pollution, MFCs have been developed rapidly in the past decade. Currently, MFCs are making the challenging step from laboratory to practical application. This paper focuses on MFC patents and the applications of MFCs. IMPACT: MFCs make it possible to directly exploit bio-electricity from organic wastes with a higher energy transforming efficiency than other traditional technologies. The wide application of MFCs will significantly reduce the energy dependence on fossil fuel as well as the relative problems of climate and environmental pollution. APPLICATIONS: MFCs have been deployed in various practical environments, such as wastewater treatment plants, seafloor, etc. The electricity generated by MFCs has been used to charge low power devices. More applications have been funded or are to be undertaken. The successful pilot applications of MFCs promise a bright future for this technology. © 2011 Society of Chemical Industry.


Wang B.,Guangdong Institute of Microbiology | Wang B.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Wang B.,Guangdong Open Laboratory of Applied Microbiology | Wang B.,CAS South China Botanical Garden | And 7 more authors.
Applied Microbiology and Biotechnology | Year: 2010

Shewanella decolorationis S12 is capable of carrying out anaerobic respiration using azo dyes and Fe (III) citrate as electron acceptors. In the present study, proteomic techniques including two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry were used to analyze the similarity and the dissimilarity of the membrane proteins isolated from strain S12 cells grown in amaranth or Fe (III) citrate with defined inorganic salt medium. The cells of strain S12 grown under a saturated dissolved oxygen condition served as controls. This is the first work that made the comparative analysis of cell membranous proteomics of strain S12 grown with azo compound or Fe (III) citrate as a sole terminal electron acceptor. The results showed that most of the membrane proteins of strain S12 under azo respiration are similar to those under Fe (III) respiration, but dissimilar from those of oxygen-grown cells. FdnH and FrdB were expressed specifically in azo respiration. NqrA-2, DctP, and hypothetical protein SO-4719 showed relative overexpression in azo respiration compared with Fe (III) respiration. OmpA family protein SO-3545 was detected to be specific to Fe (III) respiration. Furthermore, ArgF, SdhA, and HoxK were expressed markedly in both amaranth- and Fe (III) citrate-grown cultures compared with oxygen-grown cultures. © 2010 Springer-Verlag.

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