Lei J.,Jinan University |
Lei J.,Guangzhou Trauer Biotechnology Co. |
Lei J.,Guangdong Medical Collagen Engineering Technology Research and Development Center |
Liu X.-Z.,Guangzhou Trauer Biotechnology Co. |
And 6 more authors.
Chinese Journal of Tissue Engineering Research
BACKGROUND: Collagen has been shown to promote the restoration of damaged tissue wound and to accelerate wound healing. OBJECTIVE: To observe the effect of collagen gel dressing on defect healing of diabetic skin and its role to promote capillary regeneration. METHODS: Forty of 160 Sprague-Dawley rats were used as normal controls, and the other 120 rats were used to make diabetic models with intraperitoneal injection of streptozotocin. After 9 weeks, 120 model rats were randomly divided into model group, positive control group and experimental group. Then full-thickness skin wounds were made on the back of all rats. The normal group and model group were externally treated with vaseline. Experimental group was externally treated with collagen gel dressing, and recombinant human epidermal growth factor gel was used in the positive control group. Finally, the healing area of wounds and newborn capillaries were observed. RESULTS AND CONCLUSION: At 1 week of administration, animals in the normal control group showed faster wound healing, but the wound healing rates in the other groups were changed insignificantly; at 2 weeks of administration, the healing rate of the normal control group was fastest, and the nonhealing area in the model group was larger than that in the positive control and experimental groups; at 3 weeks of administration, the nonhealing area of the normal control and experimental groups was smaller than that of the model and positive control groups; at 4 weeks of administration, the nonhealing area of the normal control and positive control groups was smaller than that of the model and experimental groups. In addition, wound healing was better in the experimental group than the positive control group at 2 and 3 weeks of administration; while the number of capillaries in the experimental group was more than that of the other three groups at 1, 2, 3 weeks of administration. These results suggest that collagen gel dressings can promote healing of diabetic wounds and improve healing quality. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research All Rights Reserved. Source