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Yin J.-J.,Shantou University | Yin J.-J.,Chaonan Minsheng Hospital of Shantou | Yin J.-J.,Guangdong Key Laboratory of Medical Molecular Imaging | Ma S.-H.,Shantou University | And 15 more authors.
Clinical Imaging | Year: 2012

The study aimed to explore the abnormal activation of special brain areas associated with methamphetamine craving using functional magnetic resonance imaging (fMRI) and to reveal the neurobiological basis of addiction. Twenty-six methamphetamine addicts and 26 healthy subjects were scanned by brain fMRI while watching pictures of happy, sad, or methamphetamine to acquire resource data. SPM5 was used to analyze fMRI data to get related brain activation map, and it was found that methamphetamine addicts had high brain activation in cingulate and low activation in frontal lobe when watching methamphetamine-cue pictures. This study demonstrated that methamphetamine addicts had emotion-related brain activation abnormalities. © 2012 Elsevier Inc.


Yin J.-J.,Shantou University | Yin J.-J.,Chaonan Minsheng Hospital of Shantou | Yin J.-J.,Guangdong Key Laboratory of Medical Molecular Imaging | Liao L.-M.,Sun Yat Sen University | And 20 more authors.
Neuroendocrinology | Year: 2013

Objective: Using a block-designed BOLD-fMRI to explore the neural basis of spatial working memory impairment in patients with subclinical hypothyroidism (SCH) performing an n-back task. Methods: Sixteen patients with SCH before and after being treated with levothyroxine (LT4) for 6 months and 16 matched euthyroid subjects were scanned by fMRI under the n-back task. Results: The fMRI scan found that a neural network consisting of bilateral dorsolateral prefrontal cortex (DLPFC), bilateral premotor area (PreMA), supplementary motor area/anterior cingulate cortex, bilateral parietal lobe (PA) and right caudate nucleus/thalamus was activated, with right hemisphere dominance. In euthyroid subjects, all these regions of interest (ROIs) showed load effect; however, only left DLPFC, left PA, bilateral PreMA and right caudate nucleus/thalamus showed the same effect in Pre-SCH patients. Furthermore, activation intensities of most ROIs (especially DLPFC and right PA) for Pre-SCH patients were lower than those in the euthyroid subjects (F <3.046, p > 0.062). Importantly, after a 6-month treatment with LT4, the load effect in SCH patients appeared the same as in the euthyroid subjects in all the ROIs (F >13.176, p < 0.0001). Conclusion: Our previous study shows that verbal working memory of SCH patients is impaired with abnormal activity in bilateral frontal areas. In this study, the results indicated that SCH patients may also have spatial working memory impairments, and the altered activities of right DLPFC and right posterior parietal lobe may be one of the underlying neural mechanisms. Most importantly, this study shows that LT4 replacement therapy can improve the memory impairment and reverse the altered neural activity network. Copyright © 2012 S. Karger AG, Basel.


Liao L.-M.,Shantou University | Liao L.-M.,Guangdong Key Laboratory of Medical Molecular Imaging | Ma S.-H.,Shantou University | Ma S.-H.,Shantou Chaonan Minsheng Hospital | And 3 more authors.
Chinese Journal of Medical Imaging Technology | Year: 2010

Hepatic encephalopathy (HE) is a central nervous system dysfunction syndrome caused by various kinds of serious liver diseases. At present, the use of brain functional imaging technology, especially PET and fMRI to study brain functional metabolism and cognitive function of patients with HE has become a hot topic. Progresses of PET and fMRI researches about HE were reviewed in this paper.


Liao L.-M.,Sun Yat Sen University | Zhang X.,Sun Yat Sen University | Ren Y.-F.,Sun Yat Sen University | Sun X.-Y.,Shenyang Medical College | And 6 more authors.
PLoS ONE | Year: 2012

Background: Small cell carcinoma of the cervix (SCCC) is a very rare tumor. Due to its rarity and the long time period, there is a paucity of information pertaining to prognostic factors associated with survival. The objective of this study was to determine whether clinicopathologic finings or immunohistochemical presence of molecular markers predictive of clinical outcome in patients with SCCC. Methodology and Findings: We retrospectively reviewed a total of 293 patients with SCCC (47 patients from Cancer Center of Sun Yat-sen University in china, 71 patients from case report of china journal, 175 patients from case report in PubMed database). Of those 293 patients with SCCC, the median survival time is 23 months. The 3-year overall survival rates (OS) and 3-year disease-free survival rates (DFS) for all patients were 34.5% and 31.1%, respectively. Univariate and multivariate analysis showed that FIGO stage (IIb-IV VS I-IIa, Hazard Ratio (HR) = 3.08, 95% confidence interval (CI) of ratio = [2.05, 4.63], P<0.001), tumor mass size (≥4 cm VS <4 cm, HR = 2.37, 95% CI = [1.28, 4.36], P = 0.006) and chromogranin A (CgA) (Positive VS Negative, HR = 1.81, 95% CI = [1.12, 2.91], P = 0.015) were predictive of poor prognosis. CgA stained positive was found to be highly predictive of death in early-stage (FIGO I-IIa) patient specifically. Conclusions: Patients with SCCC have poor prognosis. FIGO stage, tumor mass size and CgA stained positive may act as a surrogate for factors prognostic of survival. CgA may serve as a useful marker in prognostic evaluation for early-stage patients with SCCC. © 2012 Liao et al.


Wang A.-H.,Nanjing Medical University | Xiao Z.-W.,Shantou No2 Peoples Hospital | Xiao Z.-W.,Guangdong Key Laboratory of Medical Molecular Imaging | Mei W.,Guangdong Key Laboratory of Medical Molecular Imaging
Chinese Journal of Medical Imaging Technology | Year: 2011

Objective: To explore the brain mechanism of arousal abnormal in emotional processing in abstinent heroin abstainer. Methods: Totally 13 heroin abstainers (experimental group) and 13 healthy subjects (control group) underwent fMRI when viewing pictures with different arousal levels. Then the arousal ratings were evaluated by the subjects. fMRI data were processed with AFNI software. Behavioral data were analyzed with SPSS 13.0 software. Results: Looking at emotional pictures, compared with control subjects, brain activation of experimental group decreased in left amygdale and hippocampus, bilateral thalamus, cingulate gyrus, bilateral superior frontal gyrus and middle frontal gyrus, right precentral gyrus, bilateral superior temporal gyrus, right inferior tempotal gyrus, left fusiform gyrus and bilateral caudate nucleus. ROI analysis indicated increased brain activation in experimental group when watching high arousal stimuli than low arousal stimuli in right thalamus, left amygdale and hippocampus, while the controls showed the opposite pattern. Conclusion: Heroin abusers have abnormalities in arousal processing.


Liao L.-M.,Shantou University | Liao L.-M.,Guangdong Key Laboratory of Medical Molecular Imaging | Liao L.-M.,Sun Yat Sen University | Zhou L.-X.,Sun Yat Sen University | And 7 more authors.
Brain Research | Year: 2012

The term "minimal hepatic encephalopathy" (MHE) refers to a population of individuals who have no recognizable clinical symptoms but perform abnormally on neuropsychological and neurophysiological tests. Research shows that MHE patients have impairments in cognition affecting their daily lives that should be treated. This study explored the neural basis of spatial working memory impairment in MHE patients using behavioral test and BOLD-fMRI. Twelve normal controls, twelve cirrhosis patients without MHE and twelve MHE patients took part. The memory quotient of the MHE group (Wechsler Memory Scale-Chinese revised: WMS-CR) was lower than the normal control group and the cirrhosis-without-MHE group, and primarily concerned short-term memory and transient memory. Performance accuracy was lower for the MHE group than the control group and the cirrhosis-without-MHE group, and mean reaction time was prolonged. The fMRI data highlighted a neural network consisting of: bilateral prefrontal cortex (PFC), bilateral premotor area (PreMA), supplementary motor area (SMA) and bilateral parietal areas (PA), which was activated in the n-back task. The load effect of BOLD-fMRI response appeared in all regions of interest (ROI) for the normal control group, but only appeared in PreMA and PA, and did not vary with n-back load in PFC or SMA for the MHE group. Activation intensities for all ROIs were higher for the normal control group than the MHE group, especially in 2-back load. In conclusion, these results demonstrate that MHE patients have debilitated spatial working memory, and that impairments of bilateral PFC, PMA, SMA, and PA commonly lead to spatial working memory dysfunction. Furthermore, PFC impairment may form the neural basis of spatial working memory impairment. © 2012 Elsevier B.V. All rights reserved.


Duan S.,Shantou University | Duan S.,Guangdong Key Laboratory of Medical Molecular Imaging | Jiang X.,Shantou University | Zhang X.,Shantou University | And 7 more authors.
Medical Science Monitor | Year: 2016

Background: Hormonal effects on the gubernaculum can affect testicular descent. Diethylstilbestrol (DES) is a nonsteroidal synthetic estrogen that disrupts the outgrowth of gubernaculums, leading to testis maldescent. However, the underlying mechanisms remain elusive. Material/Methods: The gubernaculum were removed from 3-day-old mice and cultured. The subcultured cells were randomly divided into a normal control group and experimental groups. The DES groups were administered 10 μg/ml, 1 μg/ml, 0.1 μg/ml, 0.01 μg/ml of diethylstilbestrol dissolved in dimethyl sulfoxide (DMSO) respectively. The cell morphology was observed under an inverted microscope, and leucine-rich repeat-containing G protein-coupled receptor 8 (LGR8) was localized by immunofluorescence. The expressions of LGR8 gene and protein in gubernaculum cells were quantified by RT-PCR and Flow Cytometer respectively. Results: DES treatment converted cells from a normal fibroblast-like morphology into a more refractile, spindle-shaped morphology or irregular elliptical shapes along with cytoplasmic shrinkage. LGR8 was expressed in the cytoplasmic membrane, DES dose-dependently downregulated LGR8 expression at low doses (£1.0 μg/ml), but upregulated LGR8 at high doses (10 μg/ml) at both the mRNA and protein levels. Conclusions: These results suggest that DES causes testicular maldescent by altering the LGR8 pathway in mouse gubernaculum testis cells. © Med Sci Monit.


Huang R.-R.,Shantou University | Huang R.-R.,Chaonan Minsheng Hospital of Shantou | Huang R.-R.,Guangdong Key Laboratory of Medical Molecular Imaging | Ma S.-H.,Shantou University | And 4 more authors.
Chinese Journal of Medical Imaging Technology | Year: 2013

Diabetes mellitus can cause systematic complications associated with several systems. Central cognitive impairment and complications of diabetes have become the focus of clinical practice. Progresses on structural imaging and functional imaging of cerebral cognitive impairment in diabetes mellitus, such as MRI, MRS, SPECT, PET and fMRI, were reviewed in this article. Copyright © 2013 by the Press of Chinese Journal of Medical Imaging Technology.


Liao L.-M.,Shantou University | Liao L.-M.,Guangdong Key Laboratory of Medical Molecular Imaging | Zheng M.,State Key Laboratory of Oncology in Southern China | Zheng M.,Sun Yat Sen University | And 8 more authors.
Archives of Gynecology and Obstetrics | Year: 2011

Purpose: In an attempt to understand the molecular characterization for the early adenocarcinoma of the uterine cervix, an analysis of gene expression profiles obtained from early adenocarcinoma of the uterine cervix was performed to find those genes most aberrantly expressed. Methods and materials: Total RNA was prepared from 32 samples of early adenocarcinoma of the uterine cervix and 32 paired normal cervix tissues, and hybridized to cancer-associated oligonucleotide microarrays with probe sets complementary to about 1,426 transcripts. Results: Supervised analysis of gene expression data identified 13 genes that exhibited >2-fold upregulation and 27 genes >2-fold downregulation, respectively, in early adenocarcinoma of the uterine cervix compared to normal cervix. Unsupervised hierarchical clustering of the expression data readily distinguished early adenocarcinoma of the uterine cervix from normal cervix. Two genes (karyopherin alpha 2 and proliferating cell nuclear antigen) were selected randomly for real-time reverse transcription polymerase chain reaction analysis. Both genes were expressed significantly higher in early adenocarcinoma of the uterine cervix than in normal cervix, with p = 0.0003 and <0.0001, respectively. These results were compatible with the microarray data. Conclusions: This study has revealed several genes that may be highly attractive candidate molecular markers/targets for early adenocarcinoma of the uterine cervix. © Springer-Verlag 2010.

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