Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Insts

Zhanjiang, China

Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Insts

Zhanjiang, China
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Cai S.-H.,Guangdong Ocean University | Cai S.-H.,Guangdong Provincial Key Laboratory of Pathogenic Biology | Cai S.-H.,Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Insts | Wang B.,Guangdong Ocean University | And 11 more authors.
Journal of Basic Microbiology | Year: 2012

Streptococcus iniae is a major pathogen that causes sever economic losses in tilapia aquaculture. A set of four specific primers was designed by targeting lctO gene. With Bst DNA polymerase, the target DNA can be clearly amplified for 60 min at 64 °C in a simple water bath. The sensitivity of the LAMP assay for the detection of S. iniae is about 12.4 cells per reaction in both of pure cultures and added fish tissues cultures. LAMP products could be judged with agar gel or naked eye after addition of SYBR Green I. There were no cross-reactions with other bacterial strains indicating high specificity of the LAMP. The LAMP method was also applied to detect S. iniae -infected tilapia tissues effectively. The LAMP assay reported here indicates the potential usefulness of the technique as a valuable simple, rapid alternative procedure for the detection of S. iniae during streptococcicosis monitoring of cultured fish. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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