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Zhang X.,Guangdong Institute of Microbiology
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2011

Bacteroidales has been proposed as a fecal pollution indicator. microbial source tracking (MST) based on Bacteroidales host-specific gene markers has recently been applied in the fecal pollution identification, which does not require culturing the fecal pollution indicator organisms. This method needs to design specific primers. The primers are designed based on Bacteroidales specific 16S rRNA gene. Once a pair of specific primers was amplified, the fecal pollution can be identified. In this paper, the progress of specific primers of Bacteroidales in human, swine, ruminant feces were reviewed and discussed. The advantages and disadvantages were put forward. Future researchers should be focused on the new biological markers and the combination of different MST methods. Source


Chen X.,Guangdong Institute of Microbiology
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2011

Zero-valent iron (Fe(0)) is very effective for the transformation of a wide variety of common environmental contaminants. No contaminant mineralization happened only by Fe(0) corrosion, but the pollutants would be completely detoxified and degraded by the integrated microbial-Fe(0) treatment, which represent a new generation of environmental remediation technologies. In this paper, the mechanism, microbial diversity and application of the integrated microbial-Fe(0) treatment processes were reviewed. In addition, we also discussed the main problems and challenges in this filed. Source


Xu M.,Guangdong Institute of Microbiology
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2010

Microbial eco-systems metabolic networks (MEMNs) are ubiquitous in nature and are the major drivers of biosphere processes. Here we try to define MEMNs and describe their significances in microbial ecology studies. We also review recent progresses and approaches of MEMNs including highlights of the major discoveries in these studies based on mixed-cultures, high-throughput sequencing and microarray data, computational approaches, and metabolic reconstruction. In addition, we discuss the potential shortcomings of current approaches and propose that the time is ripe to study the energy-based microbial metabolic networks in bioremediation systems, especially under the anaerobic conditions, which will reclaim the wastes to resources. Source


He M.,Xiamen University | He M.,Guangdong Institute of Microbiology | Wang W.-X.,Xiamen University | Wang W.-X.,Hong Kong University of Science and Technology
Food and Chemical Toxicology | Year: 2013

We conducted a large scale investigation of the bioaccessibility of 12 trace elements from 11 marine mollusc species (scallop, oyster, clam, abalone, snail, and mussel) collected from five locations in Chinese coastal waters. The bioaccessibility of all the 12 trace elements was generally high, with the average values ranging from 42.5% to 90.7%. The highest bioaccessibility was observed for As, Cu, Ni and Se, and the lowest for Fe, Co and Pb. Steaming decreased the bioaccessibility of all 12 trace elements and thus diminished their risks. No correlation was observed between the bioaccessibility and the total concentration of the 12 elements. However, there was a significant correlation between the bioaccessibility of the 12 elements and their subcellular distribution. For most trace elements, a significantly negative relationship was demonstrated between the bioaccessibility and the elemental partitioning in the metal-rich granule fraction or in the cellular debris fraction, and a significantly positive correlation was observed between the bioaccessibility and the elemental partitioning in the heat-stable protein fraction and in the trophically available fraction. Hence, the elemental subcellular distribution, especially the elemental partitioning in the trophically available fraction, might be a good predictor of the bioaccessibility and risks of trace elements in molluscs. © 2013 Elsevier Ltd. Source


Zhang L.,Guangdong Institute of Microbiology
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To study the secondary metabolites of the marine fungus Penicillium sp. FS60 from the South China Sea and their cytotoxicities. The compounds were isolated from the culture of strain FS60 by various chromatographic methods (silica gel, reverse silica gel, Sephadex-LH20, preparative TLC, HPLC and PTLC) and recrystallization. Their structures were identified by extensive analysis of their spectroscopic data. Compounds were tested for their cytotoxicities against SF-268, MCF-7, and NCI-H460 cell lines by SRB method. While, Compounds were tested for their antibacterial activities against S. aureus, E. coli and P. aeruginosa. Seven compounds were isolated from the culture and identified as methyl 2,4-dihydroxy-3,5,6-trimethylbenzoate (1), 4-hydroxyacetophenone (2), 5-hydroxymethyl-furoic acid (3), isochromophilones VIII (4), ergosterol (5), ergosterol peroxide (6), and cerevisterol (7). Compound 1 is isolated from the genus Penicillium for the first time. Compound 3 is demonstrated to have significant inhibition against S. aureus and P. aeruginosa. Compound 4 is demonstrated to have significant inhibition against the three cell lines. Source

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