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Mo X.,Jinan University | Gao D.,Guangdong Inspection and Quarantine Technology Center
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology | Year: 2010

We set up an SYBR Green I real-time RT-PCR method for the detection of genogroup II Norovirus, and this method's primers were encompassed the conservative region of Norovirus II. The limit of the detection was 102 copies. The standard curve's linear range was 102-106 copies, correlation coefficient was 0.9952, the slope was -2.982, and the intercept was 35.84. This method possessed specificity for genogroup II Norovirus, without any cross-reaction with rotavirus, adenovirus, hepatitis A virus or astrovirus. The coefficients of variation (CV) of the Ct values of the standard plasmid were 0.95%-1.69% (n=5) in intra-assay and 0.87%-1.24% (n=3) in inter-assay. We used this method to detect 30 shellfish samples, and found 3 samples were positive. This method is sensitive, specific and reliable for Norovirus II. It can be used to detect the Norovirus II in the shellfish rapidly. © 2010 CJB, All rights reserved. Source

Wu Z.-Z.,Zhongkai University of Agriculture and Engineering | Li H.-M.,Zhongkai University of Agriculture and Engineering | Bin S.-Y.,Zhongkai University of Agriculture and Engineering | Ma J.,Guangdong Inspection and Quarantine Technology Center | And 4 more authors.
BMC Evolutionary Biology | Year: 2014

Background: The oriental fruit fly, Bactrocera dorsalis s.s., is one of the most important quarantine pests in many countries, including China. Although the oriental fruit fly has been investigated extensively, its origins and genetic structure remain disputed. In this study, the NADH dehydrogenase subunit 1 (ND1) gene was used as a genetic marker to examine the genetic diversity, population structure, and gene flow of B. dorsalis s.s. throughout its range in China and southeast Asia. Results: Haplotype networks and phylogenetic analysis indicated two distinguishable lineages of the fly population but provided no strong support for geographical subdivision in B. philippinensis. Demographic analysis revealed rapid expansion of B. dorsalis s.s. populations in China and Southeast Asia in the recent years. The greatest amount of genetic diversity was observed in Manila, Pattaya, and Bangkok, and asymmetric migration patterns were observed in different parts of China. The data collected here further show that B. dorsalis s.s. in Yunnan, Guangdong, and Fujian Provinces, and in Taiwan might have different origins within southeast Asia. Conclusions: Using the mitochondrial ND1 gene, the results of the present study showed B. dorsalis s.s. from different parts of China to have different genetic structures and origins. B. dorsalis s.s. in China and southeast Asia was found to have experienced rapid expansion in recent years. Data further support the existence of two distinguishable lineages of B. dorsalis s.s. in China and indicate genetic diversity and gene flow from multiple origins.The sequences in this paper have been deposited in GenBank/NCBI under accession numbers KC413034-KC413367. © 2014Wu et al.; licensee BioMed Central Ltd. Source

Zhou W.,Guangdong Food and Drug Administration Center for Evaluation and Certification | Xie Z.,Guangdong Food and Drug Administration Center for Evaluation and Certification | Shao L.,Guangdong Inspection and Quarantine Technology Center
Chinese Journal of Chromatography (Se Pu) | Year: 2012

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for simultaneous determination of 13 antibiotics in oral hygiene products, including five tetracyclines, three macrolides, two quinolones, one β-lactam and two lincosamides. The sample was extracted with 0. 1% (volume percentage; same hereinafter) formic acid-acetonitrile (95: 5, v/v), then centrifuged; filtered and diluted. The target compounds were separated on a C18 column (l50 mm × 2. 1 mm; 5 μm) with a gradient elution of 0. 1% formic acid and acetonitrile as the mobile phases; and detected by tandem mass spectrometry in positive electrospray ionization and multiple reaction monitoring (MRM) mode. The quantification of 13 antibiotic was performed by the external standard method. The calibration curves showed good linearity in the range of 5.0-50. 0 μg/L with detection limits of 10. 0 mg/kg. The recoveries of Antibiotics in mouthwash and toothpaste samples at the three spiked levels of 10, 20 and I00 mg/kg were in the range of 80. 1% - 115% with the relative standard deviations in the range of 0. 94% -8. 69%. This method is accurate; reliable; simple; and suit-able for the analysis of antibiotics in oral hygiene products. Source

Yang Q.,Guangzhou Unisun Power Technology Co. | Liao Y.,Guangdong Electric Power Research Institute | Mao L.,Guangdong Inspection and Quarantine Technology Center
Chinese Journal of Chemical Engineering | Year: 2012

This study is focused on the kinetic characteristics of photocatalytic degradation of gaseous organic compounds on modified titanium dioxide/activated carbon composite photocatalyst (MTA). The MTA, which co-doping with iron (Fe) and nitrogen (N), was synthesized by a sol-gel method, and its photocatalytic performance was investigated under different reaction conditions. The experimental data obtained were tested by the zero, first and second order kinetic model, and the factors affecting the kinetic model were analyzed. It was clearly demonstrated that the experimental data of toluene and acetone on MTA fit quite well with second order kinetic model equation, but the experimental data of formaldehyde fits well with zero order kinetic model equation. © 2012 Chemical Industry and Engineering Society of China (CIESC) and Chemical Industry Press (CIP). Source

Shi Y.X.,Guangdong Inspection and Quarantine Technology Center
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases | Year: 2011

According to the sequences of small subunit ribosomal RNA (SSU rRNA) gene of Plasmodium spp., universal and species-specific primers were designed to detect malaria and identify species. 60 blood samples were detected by the established nested PCR method. The results were compared with those of microscopic examination. 40 blood samples were Plasmodium-positive by nested PCR with 22 samples of P. falciparum, 13 of P. vivas, 3 with P. falciparum and P. vivax mixed infection, 1 of P. ovale and 1 of unclassified malaria infection. Altogether, the coincidence between the results of nested PCR and microscopy stood for 76.7% (46/60), including 18 of P. falciparum, 11 of P. vivax and 17 negatives. Further sequence analysis and real-time PCR were performed to detect blood samples with discrepancy, results of which were the same as that of nested PCR. The amplified product of P. ovale was sequenced and showed 100% homology to the corresponding part of P. ovale SSU rRNA gene sequence (GenBank No. DQ845247), which confirmed that the case was imported ovale malaria. Source

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