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Liu Y.,Guangdong Ocean University | Xie Q.-Y.,Chinese Academy of Sciences | Shi W.,Guangdong Huankai Microbial Science and Technology Co. | Li L.,Wuhan University | And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

A Gram-stain positive strain, M1T, was isolated from the sediment of Maar Lake in Zhanjiang, Guangdong Province, China. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid, and mycolic acids were not detected. The polar lipid profile of strain M1T consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unknown glycolipid. The predominant quinone was MK-7, with MK-6 as a minor component. The major fatty acids were anteiso-C15:0 and anteiso-C17:0, with iso-C18:0 as a minor component. The DNA G+C content of the genomic DNA was 71.0 mol%. 16S rRNA gene sequence analysis showed that strain M1T belongs to the family Dermabacteraceae, sharing highest sequence similarity with Brachybacterium nesterenkovii JCM 11648T (98.1 %). Furthermore, a combination of DNA-DNA relatedness and physiological and biochemical properties indicated that the novel strain could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain M1T represents a novel species of the genus Brachybacterium, for which the name Brachybacterium huguangmaarense sp. nov. is proposed. The type strain is M1T (=CCTCC AB 2012866T=DSM 26370T). © 2014 IUMS. Source


Wei X.,CAS Guangzhou Institute of Chemistry | Wei X.,Guangdong Institute of Microbiology | Wei X.,University of Chinese Academy of Sciences | Ma Y.,CAS Guangzhou Institute of Chemistry | And 6 more authors.
Molecules | Year: 2015

An improved Helferich method is presented. It involves the glycosylation of 4-methyl-umbelliferone with glycosyl acetates in the presence of boron trifluoride etherate combined with triethylamine, pyridine, or 4-dimethylaminopyridine under mild conditions, followed by deprotection to give fluorogenic 4-methylumbelliferyl glycoside substrates. Due to the use of base, the glycosylation reaction proceeds more easily, is uncommonly α- or β-stereoselective, and affords the corresponding products in moderate to excellent yields (51%-94%) under appropriate conditions. © 2015 by the authors. Source


Wu H.,Guangdong Institute of Microbiology | Wu H.,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application | Wu H.,Guangdong Huankai Microbial Science and Technology Co. | Wu Q.,Guangdong Institute of Microbiology | And 6 more authors.
PLoS ONE | Year: 2016

The goal of this study was to identify Cd-resistant bacterial strains with endurance capacity and to evaluate their ability to remove cadmium ions from cadmium-polluted water. The Bacillus cereusS5 strain identified in this study had the closest genetic relationship with B. cereus sp. Cp1 and performed well in the removal of Cd2+ ions from solution. The results showed that both the live and dead biomasses of the Cd2+-tolerant B. cereus S5 strain could absorb Cd2+ ions in solution but that the live biomass of the B. cereus S5 strain outperformed the dead biomass at lower Cd2+ concentrations. An analysis of the cadmium tolerance genes of B. cereus S5 identified ATPase genes that were associated with cadmium tolerance and involved in the ATP pumping mechanism. The FTIR spectra revealed the presence of amino, carboxyl and hydroxyl groups on the pristine biomass and indicated that the cadmium ion removal ability was related to the structure of the strain. The maximum absorption capacity of the B. cereus S5 strain in viable spore biomass was 70.16 mg/g (dry weight) based on a pseudo-second-order kinetic model fit to the experimental data. The Langmuir and Langmuir-Freundlich isotherm adsorption models fit the cadmium ion adsorption data well, and the kinetic curves indicated that the adsorption rate was second-order. For Cd2+ concentrations (mg/L) of 1-109 mg/L, good removal efficiency (>80%) was achieved using approximately 3.48-10.3 g/L of active spore biomass of the B. cereus S5 strain. A cadmium-tolerant bacteria-activated carbon-immobilized column could be used for a longer duration and exhibited greater treatment efficacy than the control column in the treatment of cadmium-polluted water. In addition, a toxicity assessment using mice demonstrated that the biomass of the B. cereus S5 strain and its fermentation products were non-toxic. Thus, the isolated B. cereus S5 strain can be considered an alternative biological adsorbent for use in emergency responses to severe cadmium pollution and in the routine treatment of trace cadmium pollution. © 2016 Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source


Qin H.,Guangdong Huankai Microbial Science and Technology Co. | Deng J.-H.,Guangdong Huankai Microbial Science and Technology Co. | Fu Y.-F.,Guangdong Huankai Microbial Science and Technology Co. | Chen W.,Guangdong Huankai Microbial Science and Technology Co.
Xiandai Huagong/Modern Chemical Industry | Year: 2011

Based on the principle of the color reaction of fluoride-reagent spectrophotometric method, the visual colorimetric method and reagent quantitative packing technique are used to prepare an on-site flouride test kit. This test kit is easy to carry and use with rapid and accurate determination. The determination range of the kit is 0.05-1.2 mg/L. Source


Song J.-W.,Guangdong Huankai Microbial Science and Technology Co. | Wu Q.-P.,Guangdong Huankai Microbial Science and Technology Co. | Deng J.-H.,Guangdong Huankai Microbial Science and Technology Co. | Chen W.,Guangdong Huankai Microbial Science and Technology Co.
Xiandai Huagong/Modern Chemical Industry | Year: 2012

The stable peracetic acid is synthesized with hydrogen peroxide and glacial acetic acid as raw material. The stability, corrosive property and germicidal efficacy of the peracetic acid are studied as well. The results show that the concentration of the peracetic acid decreases by 8.71% after stored at room temperature for 360 days. After immersing in the solution containing 1500 mg/L of peracetic acid for 72 h, there is no corrosion of carbon steel coupon and stainless steel coupon, slight corrosion of aluminum coupon and moderate corrosion of copper coupon. The average killing logarithm values of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus faecalis, Clostridium perfringens exposed to solution containing peracetic acid 200 mg/L for 1 min are >5. The average killing logarithm value of spores of Bacillus subtilis var. niger exposed to the solution containing peracetic acid 1000 mg/L for 3 min is >5. The average killing logarithm value of Candida albicans exposed to the solution containing peracetic acid 400 mg/L for 5 min is >5. The average killing logarithm value of Aspergillus niger exposed to the solution containing peracetic acid 2000 mg/L for 5 min is >5. Source

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