Guangdong Hua Qing Yuan Biological Technology Co.

Meizhou, China

Guangdong Hua Qing Yuan Biological Technology Co.

Meizhou, China

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Chen J.,South China University of Technology | Chen J.,Guangdong Ocean University | Li L.,South China University of Technology | Su J.,South China University of Technology | And 5 more authors.
Journal of Agricultural and Food Chemistry | Year: 2015

Curcumin (Cur), an active ingredient from the rhizome of the plant Curcuma longa, has wide anticancer activities. However, due to its poor solubility and hence poor absorption, Cur has limited clinical applications. It is therefore important to develop an effective method to improve its absorption. Natural borneol (NB), a terpene and bicyclic organic compound, has been extensively used as a food additive, and our previous studies show that it can improve the uptake of Cur in cancer cells. However, the anticancer mechanism of NB/Cur remains unclear. In this study, the effects of NB/Cur on HepG2 cells were investigated by proteomic analysis. The results showed that 32 differentially expressed proteins identified by matrix assisted laser desorption ionization time-of-flight mass spectrometry were significantly changed after NB/Cur treated HepG2 cells for 24 h. Moreover, 17 proteins increased and 12 proteins decreased significantly. Biological progress categorization demonstrated that the identified proteins were mainly associated with cell cycle and apoptosis (28.1%). Subcellular location categorization exhibited that the identified proteins were mainly located in nucleus (28.1%) and mitochondrion (21.9%). Among of all proteins, we selected three differential proteins (hnRNPC1/C2, NPM, and PSMA5), which were associated with the p53 pathway. Down-regulation of hnRNPC1/C2 and NPM contributed to the enhancement of phosphorylated p53. Activated p53 and down-regulation of PSMA5 resulted in an increase in p21 protein. Further studies showed that NB/Cur induced reactive oxygen species (ROS) generation, indicating that ROS might be upstream of the G2/M arrest signaling pathway. In summary, the results exhibited the whole proteomic response of HepG2 cells to NB/Cur, which might lead to a better understanding of its underlying anticancer mechanisms. © 2015 American Chemical Society.


Chen J.,South China University of Technology | Li L.,South China University of Technology | Su J.,South China University of Technology | Su J.,Guangdong Hua Qing Yuan Biological Technology Co. | And 4 more authors.
PLoS ONE | Year: 2014

This study was to investigate the synergistic effect of NB/Cur on growth and apoptosis in A375 human melanoma cell line by MTT assay, flow cytometry and Western blotting. Our results demonstrated that NB effectively synergized with Cur to enhance its antiproliferative activity on A375 human melanoma cells by induction of apoptosis, as evidenced by an increase in sub-G1 cell population, DNA fragmentation, PARP cleavage and caspase activation. Further mechanistic studies by Western blotting showed that after treatment of the cells with NB/Cur, up-regulation of the expression level of phosphorylated JNK and down-regulation of the expression level of phosphorylated ERK and Akt contributed to A375 cells apoptosis. Moreover, NB also potentiated Cur to trigger intracellular ROS overproduction and the DNA damage with upregulation of the expression level of phosphorylated ATM, phosphorylated Brca1 and phosphorylated p53. The results indicate the combinational application potential of NB and Cur in treatments of cancers. © 2014 Chen et al.


Chen J.,South China University of Technology | Li L.,South China University of Technology | Su J.,South China University of Technology | Su J.,Guangdong Hua Qing Yuan Biological Technology Co. | And 2 more authors.
Food and Function | Year: 2015

Bisdemethoxycurcumin (BDCur) has been found widely in foods such as cheese, butter, etc., and in curry (powder) as a spice. It has been reported to possess anticancer activity. However, its poor absorption limited its application. Natural borneol (NB) has been used as a promoter of drug absorption and widely used in candies, beverages, baked goods, chewing gum and other foods. Thus, we investigated whether NB could potentiate the cellular uptake of BDCur, and elucidated the molecular mechanisms of their combined inhibitory effects on HepG2 cells. Our results demonstrate that NB significantly enhanced the cellular uptake of BDCur. Induction of cell cycle arrest in HepG2 cells by NB and BDCur in combination was evidenced by accumulation of the G2/M cell population. Further investigation on the molecular mechanism showed that NB and BDCur in combination resulted in a significant decrease in the expression level of Cdc2 and cyclin B. Moreover, studies also found that ROS acted as an upstream mediator in NB/BDCur-induced HepG2 cell growth inhibition and led to DNA damage with up-regulation of the expression level of phosphorylated ATM and p53. Our findings suggest that the strategy of using NB and BDCur in combination may have promising potential applications in cancer chemoprevention. This journal is © The Royal Society of Chemistry 2015.


Chen J.,South China University of Technology | Li L.,South China University of Technology | Su J.,South China University of Technology | Su J.,Guangdong Hua Qing Yuan Biological Technology Co. | And 5 more authors.
Journal of Functional Foods | Year: 2015

This study was to investigate whether natural borneol (NB) could enhance the anti-cancer effect of demethoxycurcumin (DCur) on HepG2 cell line by MTT assay, flow cytometry, and western blotting assay. Our results demonstrated that NB/DCur resulted in a significant decrease in cell viability due to pretreatment of NB enhancing the cellular uptake of DCur. Flow cytometric assay showed that NB/DCur-induced HepG2 cells growth inhibition was mainly caused by induction of G2/M arrest, as evidenced by accumulation of the G2/M cell population. Immunoblotting assay demonstrated that NB/DCur down-regulated expression levels of cdc2 and cyclin B1, which contributed to G2/M arrest. Moreover, NB/DCur elevated the level of intracellular reactive oxygen species (ROS), indicating that NB/DCur-induced G2/M arrest was achieved by triggered ROS-mediated DNA damage involving MAPK and Akt signaling pathways. Taken together, our results suggested that the combination of NB and DCur induced G2/M phase arrest in HepG2 through ROS overproduction. This study demonstrated that NB had the potential to be further developed into a chemosensitizer of DCur in the treatment of human cancers. © 2015 Elsevier Ltd.


Zhao L.,South China University of Technology | Su J.,South China University of Technology | Su J.,Guangdong Hua Qing Yuan Biological Technology Co. | Li L.,South China University of Technology | And 5 more authors.
Food Research International | Year: 2014

5-Hydroxymethylfurfural (5-HMF) has been found widely in foods through degradation of hexoses and the Maillard reaction during heat treatment of foods containing reducing sugars and amino acids in an acid environment. We have previously demonstrated 5-HMF as an antioxidant and antiproliferative ingredient. However, the action mechanisms and the underlying signaling pathways remain elusive. Therefore, in this study, experiments were carried out to elucidate the molecular mechanisms accounting for the anticancer action of 5-HMF by using MTT assay, flow cytometric and Western blot analysis. The results showed that 5-HMF exhibited a dose-dependent inhibitory effect on A375 cells, which was caused by apoptosis and G0/G1 phase arrest. Meanwhile, exposure of phosphatidylserine was observed further to prove the cell apoptosis. Further investigation on the molecular mechanisms revealed that ROS acts as an upstream mediator in 5-HMF-induced A375 cell growth inhibition. Low levels of ROS can induce DNA damage-mediated expression of P53 and phosphorylates, mitochondrial dysfunction, G0/G1 arrest and the activation of AKT and MAPKs pathways, thereby contributing to apoptosis and G0/G1 phase arrest induced by 5-HMF. Our findings imply that 5-HMF may be a candidate chemopreventive and chemotherapeutic agent because the function that induces apoptosis and/or cell cycle arrest by many anticancer drugs in susceptible cells is also exhibited by 5-HMF. © 2014 Elsevier Ltd.

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