Guangdong Food Industry Institute

Guangzhou, China

Guangdong Food Industry Institute

Guangzhou, China
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Guo Q.,Guangdong University of Technology | Yuan J.,Guangdong Food Industry Institute | Zeng J.,Guangdong University of Technology
Biotechnology and Biotechnological Equipment | Year: 2014

The binding mechanisms of the interaction of three dihydromyricetin (DMY)-metal complexes (DMY-Cu (II) complex, DMY-Mn (II) complex, DMY-Zn (II) complex) and DMY with bovine serum albumin (BSA) were investigated using fluorescence and ultraviolet spectroscopy at different temperatures. The results indicated some differences in the binding process between different DMY-metal complexes and BSA compared with that of free DMY. All of the complexes and DMY quenched the fluorescence of BSA based on static mode combined with radiationless energy transfer, yet having different binding distance based on the Förster theory. Different DMY-metal complexes can change the binding constants. The binding constants increase for DMY-Cu (II) and DMY-Mn (II) complexes, whereas the opposite is true for the DMY-Zn (II) complex compared to the one with free DMY. The DMY-metal complexes can also affect the types of the interaction. The van der Waals forces and hydrogen bonding may play a major role in the interaction of free DMY with BSA, while for the three complexes, the nature of the binding forces lies in hydrophobic forces and hydrogen bonding based on the thermodynamic parameters. © 2014 The Author(s). Published by Taylor & Francis.


PubMed | Guangdong Food Industry Institute and Guangdong University of Technology
Type: Journal Article | Journal: Biotechnology, biotechnological equipment | Year: 2015

The binding mechanisms of the interaction of three dihydromyricetin (DMY)-metal complexes (DMY-Cu (II) complex, DMY-Mn (II) complex, DMY-Zn (II) complex) and DMY with bovine serum albumin (BSA) were investigated using fluorescence and ultraviolet spectroscopy at different temperatures. The results indicated some differences in the binding process between different DMY-metal complexes and BSA compared with that of free DMY. All of the complexes and DMY quenched the fluorescence of BSA based on static mode combined with radiationless energy transfer, yet having different binding distance based on the Frster theory. Different DMY-metal complexes can change the binding constants. The binding constants increase for DMY-Cu (II) and DMY-Mn (II) complexes, whereas the opposite is true for the DMY-Zn (II) complex compared to the one with free DMY. The DMY-metal complexes can also affect the types of the interaction. The van der Waals forces and hydrogen bonding may play a major role in the interaction of free DMY with BSA, while for the three complexes, the nature of the binding forces lies in hydrophobic forces and hydrogen bonding based on the thermodynamic parameters.


Feng Z.-Q.,Guangdong Food Quality Supervision and Inspection Station | Feng Z.-Q.,Guangdong Provincial Food Industry Public Laboratory | Zhuang J.-Y.,Guangdong Food Quality Supervision and Inspection Station | Zhuang J.-Y.,Guangdong Provincial Food Industry Public Laboratory | And 4 more authors.
Modern Food Science and Technology | Year: 2014

GC-MS was applied to analyze the contents of 37 fatty acids in vegetable oils (peanut oil, colza oil, palm oil and edible blend oil), animal oils (butter, mutton fat, duck grease) and catering waste oils respectively to establish a characteristic fingerprint data base for different types of vegetable oils.Meanwhile, discrimination (distance discriminant method) and cluster (Flexible-Beta Method) analysis were conducted to determine fatty acid compositions of the abovementioned oils l. The results showed that normal oils were well classified through the function except for a small amount of adulteration and oils with high saturated fatty acid content. Twenty-three blind samples were investigated and the accuracy was as high as 91.3%. Therefore, the established model could distinguish different kinds of oil correctly based on changes in content or proportion of fatty acids.


Cui B.-Q.,Guangdong Food Industry Institute
Modern Food Science and Technology | Year: 2013

For purification total alkaloids of Nelumbonucifera leaves, capability of adsorption and desorption of three kinds of macroporous adsorption resin such as AB-8, D101 and HP-20 were explored. Then the effects of adsorption parameters, such as flow rate of adsorption, pH value, maximum loading amount of the extract solution; concentration, pH value of eluant and flow rate of desorption, on the nuciferine content were investigated by HPLC. The results showed that D101 was the most efficient Resin. The optimum condition were 2BV/h of loading rate, 10 of pH value, 6 BV of maximum loading amount of the extract solution, flow rate of desorption 2BV/h and pH 3 of the eluent (70% aqueous ethanol solution). Under such conditions, the ratio of nuciferine to the purified extract of Nelumbonucifera leaves was up to 2.26%. This research provided references for comprehensive development and utilization of the total Alkaloids of Leaves of Nelumbonucifera Gaertn.


Fang S.,South China Normal University | Liang X.,South China Normal University | Long Y.,South China Normal University | Li X.,South China Normal University | And 3 more authors.
Organometallics | Year: 2012

The asymmetric ring-opening of azabicyclic alkenes with a variety of phenols is investigated using an iridium catalyst generated in situ from 2.5 mol % of [Ir(COD)Cl] 2 and 5.0 mol % of (S)-BINAP, which afforded the corresponding 1,2-trans-phenoxyamino products in excellent yield (up to 92%) with moderate to good enantioselectivities (up to 98% ee). The trans-configuration of the product 4b was confirmed by X-ray crystallography. © 2012 American Chemical Society.


Yang D.,South China Normal University | Xia J.,South China Normal University | Long Y.,South China Normal University | Zeng Z.,South China Normal University | And 3 more authors.
Organic and Biomolecular Chemistry | Year: 2013

A novel asymmetric ring-opening reaction of N-substituted azabenzonorbornadienes with a wide variety of substituted benzyl alcohols and the addition reaction of N-substituted azabenzonorbornadienes with thiols are reported, affording the corresponding 1,2-trans-alkoxyamino products in moderate yields with excellent enantioselectivities (up to 94% ee) and the corresponding thiol addition products in high yields with lower enantiomeric excesses (ee) in the presence of iridium catalyst, respectively. The effects of ligands, catalyst loading, solvents and additives, and temperature were also investigated. The anti-configuration of the product 3c was confirmed by X-ray crystal structure analysis. A possible mechanism for the present catalytic reaction is proposed. © 2013 The Royal Society of Chemistry.


Meng L.,South China Normal University | Yang W.,South China Normal University | Pan X.,South China Normal University | Tao M.,South China Normal University | And 5 more authors.
Journal of Organic Chemistry | Year: 2015

A platinum(II)-catalyzed asymmetric ring opening of oxabenzonorbornadienes with phenols was developed, which afforded the corresponding cis-2-(un)substituted phenoxy-1,2-dihydronaphthalen-1-ol products rather than the trans ones in excellent yields (up to 99%) with moderate to good enantioselectivities (up to 87% ee) under mild conditions. In addition, the cis-configuration of product 2b was confirmed by X-ray diffraction analysis. Based on the results, a potential mechanism for the present catalytic reaction was proposed. © 2015 American Chemical Society.


Yang D.,South China Normal University | Long Y.,South China Normal University | Zhang J.,South China Normal University | Zeng H.,South China Normal University | And 2 more authors.
Organometallics | Year: 2010

We have explored a new iridium-catalyzed ring-opening reaction of oxabenzonorbornadienes with a variety of primary aromatic amine or N-substituted piperazine nucleophiles, affording the corresponding products in excellent yields (up to 99%) with moderate enantioselectivity (25-81% ee). The trans configuration of product 2d was confirmed by X-ray crystallography. © 2010 American Chemical Society.


Yang D.,South China Normal University | Long Y.,South China Normal University | Wu Y.,South China Normal University | Zuo X.,South China Normal University | And 5 more authors.
Organometallics | Year: 2010

A novel iridium-catalyzed ring-opening reaction of azabicyclic alkenes with a variety of primary aromatic amines is reported, which afforded the corresponding 1,2-trans-diamine derivatives in high yields (up to 96%) with excellent enantioselectivities (up to 97% ee) under relatively mild conditions. The trans configuration of product 2b was confirmed by X-ray crystallography. © 2010 American Chemical Society.


Trademark
Guangdong Food Industry Institute | Date: 2013-10-14

Adjuvant for use with agricultural chemicals; Alkalies; Ammoniacal salts; Anhydrous ammonia; Biochemical catalysts; Chemical fertilizers; Chemical preparations for preserving foodstuffs; Chemical preparations for preventing pathogenic infections in plants; Enzymes for use in the processing of sugar cane; Fertilizers and manures; Halides and halogen acid salts; Lactic acid; Lactose for industrial purposes; Silicon.

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