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Zhang S.-Y.,Nanjing University | Xie J.-Y.,Guangdong Experimental High School | Liang H.-W.,Nanjing University | Chen X.,Nanjing University | Zhang C.-Y.,Nanjing University
Progress in Biochemistry and Biophysics | Year: 2013

Recently, numerous studies have documented the importance of microRNAs (miRNAs) as an essential cornerstone of the genetic system. Once thought as unstable RNA molecules, miRNAs are now known to circulate in the bloodstream and other body fluids in a stable, cell-free form. Importantly, extracellular miRNAs are aberrantly present in plasma, serum and other body fluids during the pathogenesis of many diseases and, thus, are promising noninvasive or minimally invasive biomarkers to assess the pathological status of the body. However, the origin and biological function of extracellular miRNAs remains incompletely understood. In this review, we summarize the recent literature on the biogenesis and working models of extracellular miRNAs, and we highlight the impact of extending these ongoing extracellular miRNA studies to clinical applications. Source


Wang Z.,Guangdong Experimental High School | Hu Z.,Guangdong Experimental High School | Huang L.,Sun Yat Sen University
Conservation Genetics Resources | Year: 2014

Apterosperma oblata is a vulnerable species endemic to south China with highly narrow distribution. We have developed 12 polymorphic microsatellite markers using FIASCO protocol. These loci produced alleles ranging from two to seven, and expected heterozygosity from 0.105 to 0.773. Among them, seven loci were successfully cross-amplified in Euryodendron excelsum, of which three loci exhibited polymorphism. These markers will promote further investigation of adaptive evolution in A. oblata and E. excelsum, and be useful for establishing conservation strategies. © 2014, Springer Science+Business Media Dordrecht. Source


An F.F.,Guangdong Experimental High School | Liu Y.C.,Guangdong Experimental High School | Zhang W.W.,China Institute of Technology | Zhang W.W.,Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery | And 2 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2013

Dictamnine (Dic) has the ability to exert cytotoxicity in human cervix, colon, and oral carcinoma cells and dihydroartemisinin (DHA) also has potent anticancer activity on various tumour cell lines. This report explores the molecular mechanisms by which Dic treatment and combination treatment with DHA and Dic cause apoptosis in human lung adenocarcinoma A549 cells. Dic treatment induced concentration- and time-dependent cell death. FCM analysis showed that Dic induced S phase cell cycle arrest at low concentration and cell apoptosis at high concentration in which loss of mitochondrial membrane potential (Ψm) was not involved. In addition, inhibition of caspase-3 using the specific inhibitor, z-DQMD-fmk, did not attenuate Dic-induced apoptosis, implying that Dic-induced caspase-3-independent apoptosis. Combination treatment with DHA and Dic dramatically increased the apoptotic cell death compared to Dic alone. Interestingly, pretreatment with z-DQMD-fmk significantly attenuated DHA and Dic co-induced apoptosis, implying that caspase-3 plays an important role in Dic and DHA co-induced cell apoptosis. Collectively, we found that Dic induced S phase cell cycle arrest at low concentration and cell apoptosis at high concentration in which mitochondria and caspase were not involved and DHA enhanced Dic induced A549 cell apoptosis via a caspase-dependent pathway. Source

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