Guangdong Entry Exit Inspection and Quarantine Bureau

Guangzhou, China

Guangdong Entry Exit Inspection and Quarantine Bureau

Guangzhou, China
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Ye X.,Shandong Entry Exit Inspection and Quarantine Bureau | Peng Y.,Ocean University of China | Niu Z.,Shandong Entry Exit Inspection and Quarantine Bureau | Gao Y.,Shandong Entry Exit Inspection and Quarantine Bureau | And 3 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2014

A method of ultra-performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry (UPLC-LTQ/Orbitrap MS) was used to screen and confirm 24 banned aromatic amines and their 14 isomers at the same time. The main factors influencing the separation including the column, and the nature of make-up solvent were optimized. Under the optimized experimental conditions, the analytes were reduced to banned aromatic amine with sodium dithionite, extracted by methyl text-butyl ether and loaded onto a ZORBAX SB-C18 column (150 mmx2. 1 mm, 5 μm) with a gradient elution of methanol and 0. 1% formic acid aqueous solution, and finally detected by LTQ/Orbitrap MS. The screening and quantitative analysis were carried out by the accurate mass of quasi-molecular ion and the peak in extracted chro-matogram with accurate mass. The correlation coefficients were higher than 0. 99 and the limits of detection were in the range of 0. 5-5 μg/kg. The method could screen and confirm the 24 banned aromatic amines and their 14 isomers at the same time. The results were 1. 56 mg/kg of 4-chloroanihne, 0. 34 mg/kg of o-toluidine, and 0. 81 mg/kg of 2,6-toluylenediamine with the relative standard deviations ranging from 0. 27% to 1. 32% in actual samples. The results indicate that the developed method is simple, efficient and precise, and can be a reliable technique for the separation of the 24 banned aromatic amines and their 14 isomers in textile samples.


Wu Y.,China Agricultural University | Wu Y.,Chinese Academy of Sciences | Mcpheron B.A.,Pennsylvania State University | Wu J.-J.,Guangdong Entry Exit Inspection and Quarantine Bureau | Li Z.-H.,China Agricultural University
Insect Science | Year: 2012

The melon fruit fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae), has been the subject of worldwide quarantine and management efforts due to its widespread agricultural impact and potential for rapid range expansion. From its presumed native distribution in India, this species has spread throughout the hot-humid regions of the world. We provide information that reveals population structure, invasion history and population connectivity from 23 locations covering nine countries based on DNA sequences of the mitochondrial cytochrome oxidase I (COI) gene. Forty-two polymorphic sites were described among 38 haplotypes. The most common haplotype, H1, was observed in 73% of the samples distributed among all populations. Highest genetic diversity was seen within populations, and no isolation-by-distance was detected. The western regions (Nepal, Bangladesh, Thailand, Burma and China-west) showed higher haplotype diversity than eastern regions (China-east). China-Yunnan showed highest levels of genetic diversity in China. Haplotype diversity decreased with longitude from west to east. Together, these analyses suggest that B. cucurbitae has expanded from west to east within a limited geographic scale and recently invaded China through Yunnan Province. © 2012 The Authors Journal compilation © Institute of Zoology, Chinese Academy of Sciences.


Wu Y.,China Agricultural University | Li Y.,China Agricultural University | Ruiz-Arce R.,U.S. Department of Agriculture | Mcpheron B.A.,Pennsylvania State University | And 2 more authors.
Journal of Economic Entomology | Year: 2011

The melon fruit fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae), is widespread agricultural pest, and it is known to have the potential to establish invasive populations in various tropical and subtropical areas. Despite the economic risk associated with a putative stable presence of this fly, the population genetics of this pest have remained relatively unexplored in Asia, the main area for distribution of this pest. The goals for this study were to employ nuclear markers to examine geographic collections for population genetic structure and quantify the extent of gene flow within these Southeast Asian and Chinese populations. To achieve these goals, we used 12 polymorphic microsatellite markers. A low level of genetic diversity was found among collections from China and higher levels were seen in Southeast Asia collections. Three genetically distinct groups, Southeast Asia, southwest China, and southeast China, were recovered by Bayesian model-based clustering methods, the phylogenetic reconstruction and the principal coordinate analysis. The Mantel test clearly shows geographical distance contributed in the genetic structuring of B. cucurbitae's populations. No recent bottlenecks for any of the populations examined. The results of clustering, migration analyses, and Mantel test, strongly suggest that the regional structure observed may be due to geographical factors such as mountains, rivers, and islands. We found a high rate of migration in some sites from the southwest China region (cluster 1) and the southeast China region (cluster 2), suggesting that China-Guangdong- Guangzhou (GZ) may be the center of melon fruit fly in the southeast China region. © 2011 Entomological Society of America.


Li Y.,China Agricultural University | Wu Y.,China Agricultural University | Chen H.,University of Nebraska - Lincoln | Wu J.,Guangdong Entry Exit Inspection and Quarantine Bureau | Li Z.,China Agricultural University
Journal of Applied Entomology | Year: 2012

The oriental fruit fly, Bactrocera dorsalis, is a serious pest of fruits and vegetables in South-east Asia, and, because of quarantine restrictions, impedes international trade and economic development in the region. Revealing genetic variation in oriental fruit fly populations will provide a better understanding of the colonization process and facilitate the quarantine and management of this species. The genetic structure in 15 populations of oriental fruit fly from southern China, Laos and Myanmar in South-east Asia was examined with a 640-bp sequence of the mitochondrial cytochrome oxidase subunit I (COI) gene. The highest levels of genetic diversity were found in Laos and Myanmar. Low to medium levels of genetic differentiation (F ST≤0.134) were observed among populations. Pooled populations from mainland China differed from those in Laos and Myanmar (F ST=0.024). Genetic structure across the region did not follow the isolation-by-distance model. The high genetic diversity observed in Laos and Myanmar supports the South-east Asian origin of B. dorsalis. High genetic diversity and significant differentiation between some populations within mainland China indicate B. dorsalis populations have been established in the region for an extended period of time. High levels of genetic diversity observed among the five populations from Hainan Island and similarity between the Island and Chinese mainland populations indicate that B. dorsalis was introduced to Hainan from the mainland and has been on the island for many years. High genetic diversity in the recently established population in Shanghai (Pudong) suggests multiple introductions or a larger number of founders. © 2011 Blackwell Verlag, GmbH.


Zhou Y.,Guangdong Inspection and Quarantine Technology Center | Zhong B.,Guangdong Entry Exit Inspection and Quarantine Bureau | Guo R.,Guangdong Inspection and Quarantine Technology Center
18th IAPRI World Packaging Conference | Year: 2012

Using a multiple linear regression model, we analyze test results based on a sample of thousands of batches of corrugated boxes to establish the relation between compression strength and a series of variables such as bursting strength, edgewise crush strength, puncture resistance and ply adhesive strength. Based on the non-linear regression model results, we reveal that the relation between compression strength and other independent variables is more akin to that of a power function. We undertake a logarithmic transformation on the best fitting non-linear regression model to establish a formula for predicting compression strength. To test how well our formula predicts compression strength, we compare real test results with values predicted both by our formula and by traditional formulas such as those of Kellicutt, Makee and Wolf. We find the values predicted by our model are closer to those derived in real tests than are those predicted by the other three formulas considered.


Lu X.,PLA Fourth Military Medical University | Li X.,Guangdong Entry Exit Inspection and Quarantine Bureau | Mo Z.,Guangzhou University | Jin F.,PLA Fourth Military Medical University | And 4 more authors.
American Journal of Tropical Medicine and Hygiene | Year: 2012

Both Chikungunya and Dengue virus belong to the acute arthropod-borne viruses. Because of the lack of specific symptoms, it is difficult to distinguish the two infections based on clinical manifestations. To identify and quantitatively detect Chikungunya and Dengue viruses, a real-time accelerated reverse-transcription-loop-mediated isothermal amplification (RT-LAMP) platform was developed, and 26-confirmed RNA samples, 42 suspects, and 18 healthy serum samples were evaluated by the method. The RT-polymerase chain reaction (PCR) and cDNA sequencing were used as references. The results showed that it could identify the Chikungunya and Dengue virus RNA correctly in all antibody-positive samples within 1 hour, without any cross-reactions. The virus load of the positive samples was quantitatively detected with a turbidimeter. The sensitivity was 100% and specificity was 95.25%. The findings indicate that the RT-LAMP is an effective method for rapid quantity detection of Chikungunya virus and Dengue virus in serum samples with convenient operation, high specificity, and high sensitivity. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.


Lu X.,PLA Fourth Military Medical University | Li X.,Guangdong Entry Exit Inspection and Quarantine Bureau | Mo Z.,Guangzhou University | Jin F.,PLA Fourth Military Medical University | And 5 more authors.
Virus Genes | Year: 2014

A small-scale local chikungunya outbreak occurred in a Guangdong village of southern China in October 2010. The five chikungunya viruses (CHIKV) isolated from the epidemic and three other imported cases obtained from the same period were sequenced and analyzed for phylogenesis. The results demonstrated that all of the eight sequences were clustered in the Eastern, Central, Southern, and African group. However, the local strains and imported isolates showed different sequence variations. A226V in E1 gene and V264A in E2 gene were detected in all three imported isolates, the unique substitutions S250P in E1 gene and H313Y in E2 genes could be observed in four of the five local strains. These significant variations might be some of the causes for the outbreak. It would be an important event for CHIKV to have mutated adaption to the local mosquitoes in China, Aedes albopictus and Aedes aegypti. © Springer Science+Business Media 2013.


PubMed | Guangdong Entry Exit Inspection and Quarantine Bureau and Southern Medical University
Type: Journal Article | Journal: PloS one | Year: 2016

Butyrate, a short-chain fatty acid derived from dietary fiber, inhibits proliferation and induces cell death in colorectal cancer cells. However, clinical trials have shown mixed results regarding the anti-tumor activities of butyrate. We have previously shown that sodium butyrate increases endoplasmic reticulum stress by altering intracellular calcium levels, a well-known autophagy trigger. Here, we investigated whether sodium butyrate-induced endoplasmic reticulum stress mediated autophagy, and whether there was crosstalk between autophagy and the sodium butyrate-induced apoptotic response in human colorectal cancer cells.Human colorectal cancer cell lines (HCT-116 and HT-29) were treated with sodium butyrate at concentrations ranging from 0.5-5mM. Cell proliferation was assessed using MTT tetrazolium salt formation. Autophagy induction was confirmed through a combination of Western blotting for associated proteins, acridine orange staining for acidic vesicles, detection of autolysosomes (MDC staining), and electron microscopy. Apoptosis was quantified by flow cytometry using standard annexinV/propidium iodide staining and by assessing PARP-1 cleavage by Western blot.Sodium butyrate suppressed colorectal cancer cell proliferation, induced autophagy, and resulted in apoptotic cell death. The induction of autophagy was supported by the accumulation of acidic vesicular organelles and autolysosomes, and the expression of autophagy-associated proteins, including microtubule-associated protein II light chain 3 (LC3-II), beclin-1, and autophagocytosis-associated protein (Atg)3. The autophagy inhibitors 3-methyladenine (3-MA) and chloroquine inhibited sodium butyrate induced autophagy. Furthermore, sodium butyrate treatment markedly enhanced the expression of endoplasmic reticulum stress-associated proteins, including BIP, CHOP, PDI, and IRE-1a. When endoplasmic reticulum stress was inhibited by pharmacological (cycloheximide and mithramycin) and genetic (siRNA targeting BIP and CHOP) methods, the induction of BIP, PDI, IRE1a, and LC3-II was blocked, but PARP cleavage was markedly enhanced.Taken together, these results suggested that sodium butyrate-induced autophagy was mediated by endoplasmic reticulum stress, and that preventing autophagy by blocking the endoplasmic reticulum stress response enhanced sodium butyrate-induced apoptosis. These results provide novel insights into the anti-tumor mechanisms of butyric acid.


Xu J.,Guangdong Entry Exit Inspection and Quarantine Bureau | Chen C.,Guangdong Entry Exit Inspection and Quarantine Bureau | Shao L.,Guangdong Entry Exit Inspection and Quarantine Bureau | Li X.,Guangdong Entry Exit Inspection and Quarantine Bureau
Chinese Journal of Chromatography (Se Pu) | Year: 2011

A method for the simultaneous determination of metronidazole, tinidazole, ornidazole, ronidazole and dimetridazole in oral hygiene by ultra-performance liquid chromatog-raphy-tandem mass spectrometry (UPLC-MS/MS) has been developed. The Sample was diluted with 0. 1% formic acid/acetonitrile (95: 5, v/v), then centrifuged and filtered with a membrane. The separation was carried out on a Cloversil C18 cohfinn (100 mm ×2. 1 mm, 3. 5 μm) with the gradient elution of 0. 1% formic acid and acetonitrile as the mobile phases. The analytes were determined by UPLC-MS/MS and quantified by external standard method. The calibration curves showed good linearity in the range of 1. 0 -60. 0 μg/L with r≥0. 999 2. The recoveries were 91. 5% - 108% at the three spiked levels of 10. 0, 20. 0 and 100 mg/kg, and the relative standard deviations were l.14%-5. 22%. This method is easy, sensitive and suitable for the determination of nitroimidazoles in oral hygiene.


Zhuo K.,South China Agricultural University | Cui R.,South China Agricultural University | Ye W.,United Road Services | Luo M.,South China Agricultural University | And 4 more authors.
Zootaxa | Year: 2010

Aphelenchoides fujianensis n. sp. is described and illustrated from a dead Pinus massoniana based on morphology and molecular analyses of the near-full-length small subunit rDNA gene and partial cytochrome oxidase subunit I of mitochondrial DNA. This new species belongs to the Group 3 of Aphelenchoides species sensu Shahina with star-shaped tail terminus and is characterised by a relatively long body (653-843 μm in the male and 803-941 μm in the female) and four lateral incisures in the lateral field. The male has relatively large spicules (24-30 μm). The female has elongate postvulval uterine sac (extending ca 32-44% of vulva-anus distance), usually with sperms. Both male and female have star-shaped mucro. It is distinguished from other species by postvulval uterine sac length, a and c ratios, and spicule size and shape. Molecular analysis reveals that this species has unique 18S and mt-DNA sequences, and is closest to Aphelenchoides besseyi in dendrograms inferred using both markers. The identification codes of OEPP/EPPO for A. fujianensis n. sp. are: A1-B2-C1-D1/3-E1-F1/2. © 2010 Magnolia Press.

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